- Email: [email protected]
Protein technologies and commercial enzymes
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Protein technologies and commercial enzymes Web alert Sylvie Miot* and Jean-Louis Boulay† A selection of World Wide Web sites relevant to papers published in this issue of Current Opinion in Biotechnology. Addresses Department of Research, University Hospital, Hebelstrasse 20, CH-4031 Basel, Switzerland *e-mail: [email protected] †e-mail: [email protected] Current Opinion in Biotechnology 2002, 13:287–288
Enzyme Technology http://www.biores-irl.ie/biozone/enzymes.html BioResearch Ireland (BRI), the biotechnology arm of enterprise Ireland, is a partnership between government and Irish Universities to facilitate the commercialisation of opportunities arising from Irish biotechnology research. Topics treated on the educational website Biozone include enzyme biotechnology, classes of enzymes and their applications in industry, as well as novel applications and future uses in the environmental and biomedical fields. Lipase Engineering Database http://www.led.uni-stuttgart.de/ The Lipase Engineering Database compiles structural, biochemical, sequence, evolutionary and bibliographic features of mammalian and microbial lipases to facilitate protein engineering, together with a mutant database of Mucorales and Pseudomonas lipases. This site is maintained by Jürgen Pleiss and Markus Fischer, at the Institute of Technical Biochemistry, University of Stuttgart. Novozymes http://www.novozymes.com/cgi-bin/bvisapi.dll/discover/discover. jsp?cid=-9281&id=13226 The ‘discover enzymes’ section of the Novozymes website recapitulates a brief history of discoveries and innovations in enzyme research and production. Muir Lab http://chembio.rockefeller.edu/labheads/muir/muirlab.html Tom Muir, head of the Laboratory of Synthetic Protein Chemistry at Rockfeller University, has developed this website dedicated to expressed protein ligation (EPL). The hot topics and the watch video sections depict the incorporation of physical probes into proteins by EPL together with examples of applications. SomaLogic: Leading the Proteomics Revolution http://www.somalogic.com/science/science.html The science directory of SomaLogic summarises basic principles of aptamer technology, including their use for microarray screening and proteomic studies. Why Pichia pastoris? http://wwwchem.csustan.edu/chem4400/SJBR/amarjit.htm This web page contains an electronic version of the article written by Amarjit Dosanjh in the Stanislaus Journal of
Biochemical Reviews published by the California State University. This article discusses the use of Pichia pastoris as an expression system for the production of recombinant proteins. Cloning, Mutagenesis, and Structural Analysis of Human Pancreatic α-Amylase Expressed in Pichia pastoris http://www.prosci.uci.edu/Articles/Vol8/issue3/8444/8444.html This article, from Edwin Rydberg and coworkers from the University of British Columbia, Vancouver and originally published in Protein Science, describes the expression of human pancreatic α-amylase in the methylotrophic yeast Pichia pastoris. Fluorescence Resonance Energy Transfer (FRET) http://www.probes.com/handbook/boxes/0422.html This page of the web edition of the Handbook of Fluorescent Probes and Research Products from Molecular Probes comments and illustrates principles and applications of fluorescence resonance energy transfer (FRET). Protein Mutant Database http://pmd.ddbj.nig.ac.jp/~pmd/pmd.html The Protein Mutant Database (PMD) covers over 81,000 natural and artificial mutants, including random and site-directed ones, for all proteins except members of the globin and immunoglobulin families. Sequences can be retrieved by keyword or homology. When available, mutations are displayed over the three-dimensional structure of the proteins. The PMD database is managed by Ken Nishikawa and Motonori Ota at the Center for Information Biology, DNA databank of Japan, and a Japanese version of this site is available. Two-Dimensional Gel Electrophoresis Databases – Lists http://www-lmmb.ncifcrf.gov/index-ips.html#BIPDA The Image Processing Section (IPS) in the Laboratory of Experimental and Computational Biology (LECB), National Cancer Institute in Frederick, Maryland, maintains several bioinformatic databases and resources accessible on-line, including databases on two-dimensional protein gels. Other available information includes WebGel, an Internet-based gel database analysis system, Flicker, a method for comparing images from different internet sources, the 2DWG meta-database of 2D-PAGE protein gel images found on the Web, the GELLAB-II 2D gel analysis software, and access to relevant links. ExPASy Molecular Biology Server http://www.expasy.ch/ The ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics (SIB) is dedicated to the analysis of protein sequences and structures as well as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Among the directories on 2D gel analysis, are the SWISS-2D-PAGE database, and Melanie 3, the software for 2D-PAGE analysis.
288
Web alert
On-line Journals: Enzyme and Microbial Technology http://www.elsevier.com/inca/publications/store/5/2/5/0/0/4 /index.htt Protein Science http://www.proteinscience.org/ Biotechnology and Applied Biochemistry http://chemistry.about.com/gi/dynamic/offsite.htm?site=
http%3A%2F%2Fbabportlandpress.co.uk%2Fbab%2Ftoc. htm BBA: Protein Structure and Molecular Enzymology http://www.elsevier.com/gej-ng//29/50/protein/show/ Yeast http://www.interscience.wiley.com/jpages/0749-503X/ Journal of Chromatographic Science http://www.j-chrom-sci.com/home.htm
Protein technologies and commercial enzymes Web alert Sylvie Miot* and Jean-Louis Boulay† A selection of World Wide Web sites relevant to papers published in this issue of Current Opinion in Biotechnology. Addresses Department of Research, University Hospital, Hebelstrasse 20, CH-4031 Basel, Switzerland *e-mail: [email protected] †e-mail: [email protected] Current Opinion in Biotechnology 2002, 13:287–288
Enzyme Technology http://www.biores-irl.ie/biozone/enzymes.html BioResearch Ireland (BRI), the biotechnology arm of enterprise Ireland, is a partnership between government and Irish Universities to facilitate the commercialisation of opportunities arising from Irish biotechnology research. Topics treated on the educational website Biozone include enzyme biotechnology, classes of enzymes and their applications in industry, as well as novel applications and future uses in the environmental and biomedical fields. Lipase Engineering Database http://www.led.uni-stuttgart.de/ The Lipase Engineering Database compiles structural, biochemical, sequence, evolutionary and bibliographic features of mammalian and microbial lipases to facilitate protein engineering, together with a mutant database of Mucorales and Pseudomonas lipases. This site is maintained by Jürgen Pleiss and Markus Fischer, at the Institute of Technical Biochemistry, University of Stuttgart. Novozymes http://www.novozymes.com/cgi-bin/bvisapi.dll/discover/discover. jsp?cid=-9281&id=13226 The ‘discover enzymes’ section of the Novozymes website recapitulates a brief history of discoveries and innovations in enzyme research and production. Muir Lab http://chembio.rockefeller.edu/labheads/muir/muirlab.html Tom Muir, head of the Laboratory of Synthetic Protein Chemistry at Rockfeller University, has developed this website dedicated to expressed protein ligation (EPL). The hot topics and the watch video sections depict the incorporation of physical probes into proteins by EPL together with examples of applications. SomaLogic: Leading the Proteomics Revolution http://www.somalogic.com/science/science.html The science directory of SomaLogic summarises basic principles of aptamer technology, including their use for microarray screening and proteomic studies. Why Pichia pastoris? http://wwwchem.csustan.edu/chem4400/SJBR/amarjit.htm This web page contains an electronic version of the article written by Amarjit Dosanjh in the Stanislaus Journal of
Biochemical Reviews published by the California State University. This article discusses the use of Pichia pastoris as an expression system for the production of recombinant proteins. Cloning, Mutagenesis, and Structural Analysis of Human Pancreatic α-Amylase Expressed in Pichia pastoris http://www.prosci.uci.edu/Articles/Vol8/issue3/8444/8444.html This article, from Edwin Rydberg and coworkers from the University of British Columbia, Vancouver and originally published in Protein Science, describes the expression of human pancreatic α-amylase in the methylotrophic yeast Pichia pastoris. Fluorescence Resonance Energy Transfer (FRET) http://www.probes.com/handbook/boxes/0422.html This page of the web edition of the Handbook of Fluorescent Probes and Research Products from Molecular Probes comments and illustrates principles and applications of fluorescence resonance energy transfer (FRET). Protein Mutant Database http://pmd.ddbj.nig.ac.jp/~pmd/pmd.html The Protein Mutant Database (PMD) covers over 81,000 natural and artificial mutants, including random and site-directed ones, for all proteins except members of the globin and immunoglobulin families. Sequences can be retrieved by keyword or homology. When available, mutations are displayed over the three-dimensional structure of the proteins. The PMD database is managed by Ken Nishikawa and Motonori Ota at the Center for Information Biology, DNA databank of Japan, and a Japanese version of this site is available. Two-Dimensional Gel Electrophoresis Databases – Lists http://www-lmmb.ncifcrf.gov/index-ips.html#BIPDA The Image Processing Section (IPS) in the Laboratory of Experimental and Computational Biology (LECB), National Cancer Institute in Frederick, Maryland, maintains several bioinformatic databases and resources accessible on-line, including databases on two-dimensional protein gels. Other available information includes WebGel, an Internet-based gel database analysis system, Flicker, a method for comparing images from different internet sources, the 2DWG meta-database of 2D-PAGE protein gel images found on the Web, the GELLAB-II 2D gel analysis software, and access to relevant links. ExPASy Molecular Biology Server http://www.expasy.ch/ The ExPASy (Expert Protein Analysis System) proteomics server of the Swiss Institute of Bioinformatics (SIB) is dedicated to the analysis of protein sequences and structures as well as two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Among the directories on 2D gel analysis, are the SWISS-2D-PAGE database, and Melanie 3, the software for 2D-PAGE analysis.
288
Web alert
On-line Journals: Enzyme and Microbial Technology http://www.elsevier.com/inca/publications/store/5/2/5/0/0/4 /index.htt Protein Science http://www.proteinscience.org/ Biotechnology and Applied Biochemistry http://chemistry.about.com/gi/dynamic/offsite.htm?site=
http%3A%2F%2Fbabportlandpress.co.uk%2Fbab%2Ftoc. htm BBA: Protein Structure and Molecular Enzymology http://www.elsevier.com/gej-ng//29/50/protein/show/ Yeast http://www.interscience.wiley.com/jpages/0749-503X/ Journal of Chromatographic Science http://www.j-chrom-sci.com/home.htm