- Email: [email protected]
PS227 Silica Nanoparticles Induces Cardiotoxicity Interfering With CA2+ Handling in Adult Rat Cardiomyocytes
without evidence of aortic coarctation or supravalvular aortic stenosis. we ruled out differential diagnosis of fetal cardiomyopathy (diabetes, hypothyroidism) without evidence of these diseases in our patient. At birth without complications.
PS227 Silica Nanoparticles Induces Cardiotoxicity Interfering With CA2+ Handling in Adult Rat Cardiomyocytes C. E. Guerrero-Beltrán*1,2, J. Bernal-Ramírez1, A. García-García3, O. Lozano1, G. Torre-Amione1,4, N. Ornelas-Soto5, G. García-Rivas1,2 1 Cardiología y Medicina Vascular, Tecnológico de Monterrey, Campus Monterrey, Monterrey, 2 Centro de Investigación Básica y Transferencia., Hospital Zambrano-Hellion. Tecnológico de Monterrey., San Pedro Garza García, 3Centro de Investigación en Materiales Avanzados S.C., Apodaca, 4Centro de Investigación Básica y Transferencia., Hospital Zambrano-Hellion. Tecnológico de Monterrey., San Pedro Garza García, 5Escuela de Ingeniería y Ciencias., Tecnológico de Monterrey, Campus Monterrey, Monterrey, Mexico Introduction: Nanomaterials such as Silica (silicon dioxide, SiO2) are used to improve or create new functional properties to regular products, however, recently there has been enormous evidence showing that nanoparticles might exert potential risks to environment, food chain and human health. Several studies have shown that nanoparticles can penetrate to the body by different mechanisms and routes such as the skin, the respiratory system or the gastrointestinal tract, and translocate to different organs through the circulatory system reaching important organs such as the heart, lungs and kidneys. Objectives: In this study, we explored for the first time, SiO2-induced toxicity on isolated rat cardiomyocytes exposed to a 24 h incubation with 7 nm or 670 nm SiO2 particles. Methods: Silica nanoparticles was characterized by scanning electron microscope (SEM), dynamic light scattering and zeta potential. Results: SiO2 particles showed a dose-dependent cytotoxic effect on cardiomyocytes with a half maximal inhibitory concentration for the 7 nm (99.5 12.4 mg/ml) and 670 nm (>1500 mg/ml), showing a size-dependent toxicity. Both particles exerted cellular death by necrosis, in a time-dependence manner, where apoptosis was not evident. We found SiO2 membrane association and cellular internalization in a dose-dependence manner, analyzed by particle-induced X-ray emission (PIXE), SEM and confocal microscopy. Also, an impaired contractility was observed in SiO2-treated cardiomyocytes. Peak sarcomere shortening was significantly reduced 25 and 30 % after SiO2 particles exposure. Intracellular Ca2+transient was reduced after SiO2 treatment and remarkably during b-adrenergic stimulation. Time to 50 % of Ca2+ decay was reduced by SiO2 treatment, however, sarcoplasmic reticulum (SR) Ca2+ content (caffeine-induced Ca2+ transient) were unchanged. In addition, significant glutathione depletion and reactive oxygen species generation was observed in both treatments, associates with mitochondrial damage seen as a reduction on the membrane potential and a subsequence reduction in ATP content. Conclusion: Silica particles exerted cellular damage on the adult rat cardiomyocytes, in a doses dependence manner, caused by a raise in oxidative stress, leading to a mitochondrial dysfunction and a low in the energetic status, interfering directly with the Ca2+ handling due by SR Ca2+-ATPase. Disclosure of Interest: None Declared PS229 Hypertrophic Cardiomyopathy Intrauterine, Firts Case in Mexico K. Y. Juarez*1, F. J. Hernandez Leon1, M. E. Guevara Valdivia1, D. A. Aguilar Pulido1 1 Cardiology, Instituto Mexicano del Seguro Social, Mexico City, Mexico Introduction: The prevalence of hypertrophic cardiomyopathy and pregnancy is less than 0.5 - 1 %, and there are not reports in utero worldwide. Objectives: Female of 36 years old, with diagnosis of hypertrophic cardiomyopathy login to examination for pregnancy on her last month, she had a history record of familial cardiomiopathy, we performed an echocardiogram with evidence of hypertrophic cardiomiopathy without of obstruction of left tract outflow, free of symptoms Methods: We decide to make a fetal echocardiogram with evidence of septal hypertrophy: lenght 8.5 mm (maximum 3.9mm ), with anterior movement of anterior mitral leaflet,
GHEART Vol 11/2S/2016
j
June, 2016
j
POSTER/WCC_2016-POSTERS
Results: The fetal prognosis in most cases does not seem to be affected by mother pathology. Conclusion: There are not isolated reports of fetal hypertrophy heart disease, so it must be intentionally sought in these patients. Disclosure of Interest: None Declared PS230 Interleukin-27 Polymorphisms are Associated With Premature Coronary Artery Disease and Insulin Resistance. The Genetics of Atherosclerotic Disease (GEA) Mexican Study R. Posadas-Sánchez*1, N. Pérez-Hernández2, J. M. Rodríguez-Pérez2, J. Ángeles-Martínez2, C. Posadas-Romero1, G. C. Cardoso-Saldaña1, A. Medina-Urrutia1, M. T. Villarreal-Molina3, G. Vargas-Alarcón2 1 Endocrinología, 2Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, 3 Instituto de Medicina Genómica, México, Mexico Introduction: Coronary artery disease (CAD) is an immune-mediated chronic inflammatory disease. The interleukin 27 (IL-27) has a dual role in immune response with both proand anti-inflammatory properties. Some studies suggest that IL-27 is involved in CAD, however, no previous studies have examined the association of IL-27 gene polymorphisms (IL27p28) with premature CAD. Objectives: To evaluate the role of IL-27p28 gene polymorphisms as susceptibility markers for premature CAD and/or cardiometabolic risk factors. Methods: Using an informatics analysis, five IL-27p28 gene polymorphisms with possible functional effect (rs26528, rs17855750, rs181206, rs40837 and rs153109) were selected. The polymorphisms were genotyped in 1162 patients with premature CAD and 1107 controls (calcium score¼0 assessed by computed tomography). Clinical, anthropometric and biochemical measurements were performed. The association between the IL-27p28 gene polymorphisms with premature CAD and cardiometabolic risk factors was evaluated using logistic regression analyses. Results: When compared to controls, the rs26528 T (OR ¼ 0.794, CI: 0.634-0.996, Pdom¼0.046; OR ¼ 0.701, CI: 0.562-0.875], Phet¼0.002; OR ¼ 0.718, CI: 0.564-0.913, Pco-dom¼0.007) and rs40837A (OR ¼ 0.740, CI: 0.593-0.923, Phet¼0.008; OR ¼ 0.768, CI: 0.604-0.977, Pco-dom¼0.031) alleles were significantly associated with decreased risk of premature CAD. All models were adjusted by age, gender, body mass index (BMI), smoking, total abdominal fat, HOMA-IR, aspartate aminotransferase, adiponectin and uric acid levels. The rs40837A allele was significantly associated with decreased risk to present insulin resistance (IR) in both, CAD patients (OR ¼ 0.702, CI: 0.504-0.978, Phet¼0.037) and control subjects (OR ¼ 0.646, CI: 0.468-0.892, Pad¼0.008; OR ¼ 0.616, CI: 0.3810.994, Pdom¼0.047; OR ¼ 0.488, CI: 0.274-0.867, Prec¼0.014; OR ¼ 0.404, CI: 0.2100.775, Pco-dom2¼0.006). Only in control subjects the rs26528T allele was associated with IR (OR ¼ 0.623, CI: 0.423-0.916, Prec¼0.016; OR ¼ 0.610, CI: 0.401-0.930, Pco-dom2¼0.021). The models were adjusted by age, gender and BMI. Conclusion: For the first time, our study shows that the IL-27p28 gene polymorphisms are associated with premature CAD and IR. Our data suggest that rs26528T and rs40837A alleles may be considered protective markers for developing CAD and IR. Disclosure of Interest: None Declared
e51
POSTER ABSTRACTS
Results: A total of 3,220 PCI procedures (97.4%) were included in this study. The PCI procedures were mainly from National Heart Institute (48.4%), followed by University Malaya Medical Centre (UMMC) which constituted 15.8%, Sultanah Bahiyah Hospital (9.9%) and Sarawak General Hospital Heart Centre (9.3%). Majority of the PCI (60-80%) were elective cases. There is an increasing trend of number of procedures in January 2014 (n¼483) and June 2014 (n¼561). Programme costs varied considerably across all facilities, and consumables alone contribute an average 69.41%, with median RM 8,437.82 (RM 7,566.98 - 13,070.58). The estimated costs of PCI in this study, median RM 13,876.67 (RM11,518.00– 15,025.04). Significantly lower costing is found in hospital which performed more numbers of elective cases in daycare. The overall inhospital all-cause mortality (including emergency cases) was 3.2% (n¼57) from January until June 2014. The discrepancy of mortality rate was mainly contributed by the emergency PCI cases. Conclusion: The six selected public, institution and teaching hospitals showed an increased number of PCI procedures performed in mid-year as compared to earlier part of the year. The overall in-hospital all-cause mortality remained acceptably low but the scarcity of operational cost is non-related factor. Disclosure of Interest: None Declared
PS227 Silica Nanoparticles Induces Cardiotoxicity Interfering With CA2+ Handling in Adult Rat Cardiomyocytes C. E. Guerrero-Beltrán*1,2, J. Bernal-Ramírez1, A. García-García3, O. Lozano1, G. Torre-Amione1,4, N. Ornelas-Soto5, G. García-Rivas1,2 1 Cardiología y Medicina Vascular, Tecnológico de Monterrey, Campus Monterrey, Monterrey, 2 Centro de Investigación Básica y Transferencia., Hospital Zambrano-Hellion. Tecnológico de Monterrey., San Pedro Garza García, 3Centro de Investigación en Materiales Avanzados S.C., Apodaca, 4Centro de Investigación Básica y Transferencia., Hospital Zambrano-Hellion. Tecnológico de Monterrey., San Pedro Garza García, 5Escuela de Ingeniería y Ciencias., Tecnológico de Monterrey, Campus Monterrey, Monterrey, Mexico Introduction: Nanomaterials such as Silica (silicon dioxide, SiO2) are used to improve or create new functional properties to regular products, however, recently there has been enormous evidence showing that nanoparticles might exert potential risks to environment, food chain and human health. Several studies have shown that nanoparticles can penetrate to the body by different mechanisms and routes such as the skin, the respiratory system or the gastrointestinal tract, and translocate to different organs through the circulatory system reaching important organs such as the heart, lungs and kidneys. Objectives: In this study, we explored for the first time, SiO2-induced toxicity on isolated rat cardiomyocytes exposed to a 24 h incubation with 7 nm or 670 nm SiO2 particles. Methods: Silica nanoparticles was characterized by scanning electron microscope (SEM), dynamic light scattering and zeta potential. Results: SiO2 particles showed a dose-dependent cytotoxic effect on cardiomyocytes with a half maximal inhibitory concentration for the 7 nm (99.5 12.4 mg/ml) and 670 nm (>1500 mg/ml), showing a size-dependent toxicity. Both particles exerted cellular death by necrosis, in a time-dependence manner, where apoptosis was not evident. We found SiO2 membrane association and cellular internalization in a dose-dependence manner, analyzed by particle-induced X-ray emission (PIXE), SEM and confocal microscopy. Also, an impaired contractility was observed in SiO2-treated cardiomyocytes. Peak sarcomere shortening was significantly reduced 25 and 30 % after SiO2 particles exposure. Intracellular Ca2+transient was reduced after SiO2 treatment and remarkably during b-adrenergic stimulation. Time to 50 % of Ca2+ decay was reduced by SiO2 treatment, however, sarcoplasmic reticulum (SR) Ca2+ content (caffeine-induced Ca2+ transient) were unchanged. In addition, significant glutathione depletion and reactive oxygen species generation was observed in both treatments, associates with mitochondrial damage seen as a reduction on the membrane potential and a subsequence reduction in ATP content. Conclusion: Silica particles exerted cellular damage on the adult rat cardiomyocytes, in a doses dependence manner, caused by a raise in oxidative stress, leading to a mitochondrial dysfunction and a low in the energetic status, interfering directly with the Ca2+ handling due by SR Ca2+-ATPase. Disclosure of Interest: None Declared PS229 Hypertrophic Cardiomyopathy Intrauterine, Firts Case in Mexico K. Y. Juarez*1, F. J. Hernandez Leon1, M. E. Guevara Valdivia1, D. A. Aguilar Pulido1 1 Cardiology, Instituto Mexicano del Seguro Social, Mexico City, Mexico Introduction: The prevalence of hypertrophic cardiomyopathy and pregnancy is less than 0.5 - 1 %, and there are not reports in utero worldwide. Objectives: Female of 36 years old, with diagnosis of hypertrophic cardiomyopathy login to examination for pregnancy on her last month, she had a history record of familial cardiomiopathy, we performed an echocardiogram with evidence of hypertrophic cardiomiopathy without of obstruction of left tract outflow, free of symptoms Methods: We decide to make a fetal echocardiogram with evidence of septal hypertrophy: lenght 8.5 mm (maximum 3.9mm ), with anterior movement of anterior mitral leaflet,
GHEART Vol 11/2S/2016
j
June, 2016
j
POSTER/WCC_2016-POSTERS
Results: The fetal prognosis in most cases does not seem to be affected by mother pathology. Conclusion: There are not isolated reports of fetal hypertrophy heart disease, so it must be intentionally sought in these patients. Disclosure of Interest: None Declared PS230 Interleukin-27 Polymorphisms are Associated With Premature Coronary Artery Disease and Insulin Resistance. The Genetics of Atherosclerotic Disease (GEA) Mexican Study R. Posadas-Sánchez*1, N. Pérez-Hernández2, J. M. Rodríguez-Pérez2, J. Ángeles-Martínez2, C. Posadas-Romero1, G. C. Cardoso-Saldaña1, A. Medina-Urrutia1, M. T. Villarreal-Molina3, G. Vargas-Alarcón2 1 Endocrinología, 2Biología Molecular, Instituto Nacional de Cardiología Ignacio Chávez, 3 Instituto de Medicina Genómica, México, Mexico Introduction: Coronary artery disease (CAD) is an immune-mediated chronic inflammatory disease. The interleukin 27 (IL-27) has a dual role in immune response with both proand anti-inflammatory properties. Some studies suggest that IL-27 is involved in CAD, however, no previous studies have examined the association of IL-27 gene polymorphisms (IL27p28) with premature CAD. Objectives: To evaluate the role of IL-27p28 gene polymorphisms as susceptibility markers for premature CAD and/or cardiometabolic risk factors. Methods: Using an informatics analysis, five IL-27p28 gene polymorphisms with possible functional effect (rs26528, rs17855750, rs181206, rs40837 and rs153109) were selected. The polymorphisms were genotyped in 1162 patients with premature CAD and 1107 controls (calcium score¼0 assessed by computed tomography). Clinical, anthropometric and biochemical measurements were performed. The association between the IL-27p28 gene polymorphisms with premature CAD and cardiometabolic risk factors was evaluated using logistic regression analyses. Results: When compared to controls, the rs26528 T (OR ¼ 0.794, CI: 0.634-0.996, Pdom¼0.046; OR ¼ 0.701, CI: 0.562-0.875], Phet¼0.002; OR ¼ 0.718, CI: 0.564-0.913, Pco-dom¼0.007) and rs40837A (OR ¼ 0.740, CI: 0.593-0.923, Phet¼0.008; OR ¼ 0.768, CI: 0.604-0.977, Pco-dom¼0.031) alleles were significantly associated with decreased risk of premature CAD. All models were adjusted by age, gender, body mass index (BMI), smoking, total abdominal fat, HOMA-IR, aspartate aminotransferase, adiponectin and uric acid levels. The rs40837A allele was significantly associated with decreased risk to present insulin resistance (IR) in both, CAD patients (OR ¼ 0.702, CI: 0.504-0.978, Phet¼0.037) and control subjects (OR ¼ 0.646, CI: 0.468-0.892, Pad¼0.008; OR ¼ 0.616, CI: 0.3810.994, Pdom¼0.047; OR ¼ 0.488, CI: 0.274-0.867, Prec¼0.014; OR ¼ 0.404, CI: 0.2100.775, Pco-dom2¼0.006). Only in control subjects the rs26528T allele was associated with IR (OR ¼ 0.623, CI: 0.423-0.916, Prec¼0.016; OR ¼ 0.610, CI: 0.401-0.930, Pco-dom2¼0.021). The models were adjusted by age, gender and BMI. Conclusion: For the first time, our study shows that the IL-27p28 gene polymorphisms are associated with premature CAD and IR. Our data suggest that rs26528T and rs40837A alleles may be considered protective markers for developing CAD and IR. Disclosure of Interest: None Declared
e51
POSTER ABSTRACTS
Results: A total of 3,220 PCI procedures (97.4%) were included in this study. The PCI procedures were mainly from National Heart Institute (48.4%), followed by University Malaya Medical Centre (UMMC) which constituted 15.8%, Sultanah Bahiyah Hospital (9.9%) and Sarawak General Hospital Heart Centre (9.3%). Majority of the PCI (60-80%) were elective cases. There is an increasing trend of number of procedures in January 2014 (n¼483) and June 2014 (n¼561). Programme costs varied considerably across all facilities, and consumables alone contribute an average 69.41%, with median RM 8,437.82 (RM 7,566.98 - 13,070.58). The estimated costs of PCI in this study, median RM 13,876.67 (RM11,518.00– 15,025.04). Significantly lower costing is found in hospital which performed more numbers of elective cases in daycare. The overall inhospital all-cause mortality (including emergency cases) was 3.2% (n¼57) from January until June 2014. The discrepancy of mortality rate was mainly contributed by the emergency PCI cases. Conclusion: The six selected public, institution and teaching hospitals showed an increased number of PCI procedures performed in mid-year as compared to earlier part of the year. The overall in-hospital all-cause mortality remained acceptably low but the scarcity of operational cost is non-related factor. Disclosure of Interest: None Declared