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PT03.3: The Role of Three Different Lycopene Extracts on Human Lung Adenocarcinoma Cell Line
Poster Tour 3: Micronutrients 1 PT03.3 THE ROLE OF THREE DIFFERENT LYCOPENE EXTRACTS ON HUMAN LUNG ADENOCARCINOMA CELL LINE A. Bruno1, E. Pace1, M. Durante2, P. P. Marrese3, G. Mita2, L. Siena1, C. Di Sano1, M. S. Lenucci3. 1Institute of Biomedicine and Molecular Immunology - IBIM -, Consiglio Nazionale Delle Ricerche - CNR -, Palermo, 2Istituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale Delle Ricerche - CNR -, 3 Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (Di.S.Te.B.A.), Università del Salento, Lecce, Italy Rationale: Lycopene is an antioxidant carotenoid pigment found in a wide variety of vegetables and fruits, together with other lipophilic phytochemicals. Antioxidants have different effects on cancer progression. Non-small cell lung cancer (NSCLC) accounts for >80% of all lung cancers. Inflammation is a key event in cancer development. This study aims to evaluate in vitro the effects of administration of three different oleoresins containing lycopene on human NSCLC cell line, A549. Methods: The oleoresins, obtained by the supercritical CO2 green extraction technology from watermelon (Lyc W), GAC (Lyc G) and tomato (Lyc T), were chlatrated in α-cyclodextrins (α-CD) in order to improve their stability and promote the dispersion of the lipophilic compounds in the cell-culture aqueous medium. They were tested at different concentrations (from 0.5 to 10 µM lycopene) on A549 by the Cell Proliferation Assay (MTS) for 24 hrs, Annexin V-FITC/PI apoptosis test and ELISA for Interleukin-8 (IL-8) release, for 48 and 72 hrs. Results: The oleoresins were characterized by a different composition in carotenoids and tocochromanols, with Lyc W showing the highest lycopene/tocochromanol ratio. At 10 µM, the three extracts showed conflicting behaviors: Lyc W increased cell apoptosis ( p = 0.01) and decreased cell proliferation ( p = 0.009) and IL-8 release ( p = 0.03), whereas Lyc G and Lyc T decreased cell apoptosis ( p = 0.01 and p = 0.04, respectively) and increased cell proliferation ( p = 0.01 and p = 0.04, respectively) and IL-8 release (both p = 0.01). Furthermore, at 3 µM, Lyc G and Lyc T decreased cell apoptosis ( p = 0.009 and p = 0.01, respectively). Conclusion: In Lyc W the highest amount of lycopene is able to counteract and revert the survival effect of tocochromanols, supporting the importance to evaluate the real effect of antioxidant supplementation on lung cancer which not only may have no anticancer benefits but even increase cancer aggressivity. Disclosure of Interest: None declared
PT03.4 SERUM 25 HYDROXYVITAMIN D POSITIVELY ASSOCIATED WITH DIETARY VITAMIN D INTAKE AMONG BRAZILIAN ADOLESCENTS K. V. Giudici1, M. A. P. Lopes1, R. M. Fisberg1, L. A. Martini1. 1 School of Public Health/Nutrition Department, University of São Paulo, São Paulo, Brazil Rationale: Vitamin D deficiency is a highly prevalent public health concern. This study aimed to investigate associations between serum 25 hydroxyvitamin D [25(OH)D] and dietary vitamin D (calciferol) intake among Brazilian adolescents and to estimate inadequate dietary intake of this nutrient.
S27 Methods: Cross-sectional study with 198 adolescents 14–18 years old living in São Paulo, Brazil, not in use of nutritional supplements. Serum 25(OH)D was obtained by high performance liquid chromatography. Dietary vitamin D intake was measured by a 24 h food record, repeated in 62.6% of the sample. Intrapersonal variability of intake was corrected using the Multiple Source Method (MSM) software. Estimated inadequate intake was assessed by PC-Side software, based on Estimated Average Requirement (EAR) values. Pearson correlation and multiple linear regression analysis were performed. Results: Participants of the study (mean age 16.3 years, standard deviation − SD = 1.4; 51% male) presented mean calciferol intake of 1.5 μg/1,000 kcal (SD = 0.4), and 100% of the sample presented vitamin D intake below the recommendation. Serum 25(OH)D positively correlated with calciferol intake (r = 0.175, p = 0.014), and this association persisted after adjusting for sex, sun exposure and season of the year in linear regression analysis ( partial r2 = 0.026; p = 0.024). Conclusion: Despite the high prevalence of inadequate vitamin D intake, serum 25(OH)D positively associated with calciferol from diet. This result illustrates the importance of stimulating the intake of vitamin D through natural food sources, or with fortified products, if available. Additionally, the adoption of a healthy lifestyle with safe – but regular – sun exposure should also be encouraged. Disclosure of Interest: K. Giudici Grant/Research Support from: FAPESP 2011/22768-2, M. Lopes: None declared, R. Fisberg: None declared, L. Martini Grant/Research Support from: FAPESP 2012/ 11061-8
PT03.5 CONTRIBUTION OF REDUCED POST-OPERATIVE INTESTINAL CERULOPLASMIN GENE EXPRESSION FOLLOWING ROUX-EN-Y GASTRIC BYPASS SURGERY TO COPPER AND IRON DEFICIENCY P. Sala1, G. Belarmino1, N. M. Machado1, D. C. Fonseca1, R. K. Ishida1, I. F. M. S. Guarda1, E. G. H. Moura1, P. Sakai1, M. A. Santo1, I. D. C. G. Silva2, S. B. Heymsfield3, R. S. M. M. Torrinhas1, D. Giannella-Neto4, D. L. Waitzberg1. 1University of São Paulo - Medical School, 2UNIFESP - Federal University of São Paulo, São Paulo, Brazil; 3Pennington Biomedical Research Center, Baton Rouge, United States; 4University Nove de Julho, São Paulo, Brazil Rationale: Copper and iron deficiencies are the most common complications following Roux-en-Y Gastric Bypass (RYGB). There is a potential link between iron and copper metabolism through the enzyme ceruloplasmin (CP). CP is the main coppercontaining protein in blood and has copper-dependent oxidase activity that appears to influence Fe2+ (ferrous iron) oxidation to Fe3+ (ferric iron) that facilitates iron transport. We examined gene expression related to copper and iron metabolism in subject treated with RYGB; our focus was on the expression of CP-encoding genes. Methods: Intestinal biopsies were acquired through doubleballoon endoscopy in 20 obese women (age, 46.9 ± 6.2 yrs; BMI, 46.5 ± 5.3 kg/m2) before and 3 months after RYGB (BMI, 38.2 ± 4.2 kg/m2). Gut microarray analyses were performed on all samples using a Human GeneChip 1.0 STarray (Affymetrix, Inc., Santa Clara, CA) followed by Taqman® RT-qPCR analysis (Life Technologies, USA), for validation.
PT03.4 SERUM 25 HYDROXYVITAMIN D POSITIVELY ASSOCIATED WITH DIETARY VITAMIN D INTAKE AMONG BRAZILIAN ADOLESCENTS K. V. Giudici1, M. A. P. Lopes1, R. M. Fisberg1, L. A. Martini1. 1 School of Public Health/Nutrition Department, University of São Paulo, São Paulo, Brazil Rationale: Vitamin D deficiency is a highly prevalent public health concern. This study aimed to investigate associations between serum 25 hydroxyvitamin D [25(OH)D] and dietary vitamin D (calciferol) intake among Brazilian adolescents and to estimate inadequate dietary intake of this nutrient.
S27 Methods: Cross-sectional study with 198 adolescents 14–18 years old living in São Paulo, Brazil, not in use of nutritional supplements. Serum 25(OH)D was obtained by high performance liquid chromatography. Dietary vitamin D intake was measured by a 24 h food record, repeated in 62.6% of the sample. Intrapersonal variability of intake was corrected using the Multiple Source Method (MSM) software. Estimated inadequate intake was assessed by PC-Side software, based on Estimated Average Requirement (EAR) values. Pearson correlation and multiple linear regression analysis were performed. Results: Participants of the study (mean age 16.3 years, standard deviation − SD = 1.4; 51% male) presented mean calciferol intake of 1.5 μg/1,000 kcal (SD = 0.4), and 100% of the sample presented vitamin D intake below the recommendation. Serum 25(OH)D positively correlated with calciferol intake (r = 0.175, p = 0.014), and this association persisted after adjusting for sex, sun exposure and season of the year in linear regression analysis ( partial r2 = 0.026; p = 0.024). Conclusion: Despite the high prevalence of inadequate vitamin D intake, serum 25(OH)D positively associated with calciferol from diet. This result illustrates the importance of stimulating the intake of vitamin D through natural food sources, or with fortified products, if available. Additionally, the adoption of a healthy lifestyle with safe – but regular – sun exposure should also be encouraged. Disclosure of Interest: K. Giudici Grant/Research Support from: FAPESP 2011/22768-2, M. Lopes: None declared, R. Fisberg: None declared, L. Martini Grant/Research Support from: FAPESP 2012/ 11061-8
PT03.5 CONTRIBUTION OF REDUCED POST-OPERATIVE INTESTINAL CERULOPLASMIN GENE EXPRESSION FOLLOWING ROUX-EN-Y GASTRIC BYPASS SURGERY TO COPPER AND IRON DEFICIENCY P. Sala1, G. Belarmino1, N. M. Machado1, D. C. Fonseca1, R. K. Ishida1, I. F. M. S. Guarda1, E. G. H. Moura1, P. Sakai1, M. A. Santo1, I. D. C. G. Silva2, S. B. Heymsfield3, R. S. M. M. Torrinhas1, D. Giannella-Neto4, D. L. Waitzberg1. 1University of São Paulo - Medical School, 2UNIFESP - Federal University of São Paulo, São Paulo, Brazil; 3Pennington Biomedical Research Center, Baton Rouge, United States; 4University Nove de Julho, São Paulo, Brazil Rationale: Copper and iron deficiencies are the most common complications following Roux-en-Y Gastric Bypass (RYGB). There is a potential link between iron and copper metabolism through the enzyme ceruloplasmin (CP). CP is the main coppercontaining protein in blood and has copper-dependent oxidase activity that appears to influence Fe2+ (ferrous iron) oxidation to Fe3+ (ferric iron) that facilitates iron transport. We examined gene expression related to copper and iron metabolism in subject treated with RYGB; our focus was on the expression of CP-encoding genes. Methods: Intestinal biopsies were acquired through doubleballoon endoscopy in 20 obese women (age, 46.9 ± 6.2 yrs; BMI, 46.5 ± 5.3 kg/m2) before and 3 months after RYGB (BMI, 38.2 ± 4.2 kg/m2). Gut microarray analyses were performed on all samples using a Human GeneChip 1.0 STarray (Affymetrix, Inc., Santa Clara, CA) followed by Taqman® RT-qPCR analysis (Life Technologies, USA), for validation.