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RADIOGRAPHIC FINDINGS IN PATIENTS WITH MEDICATION-RELATED OSTEONECROSIS OF THE JAW
ORAL AND MAXILLOFACIAL PATHOLOGY e126 Abstracts significant correlations between LVD and MC counts. Our results suggest that intratumoral lymphatic vessels contribute to the progression of OTSCC and PLD may be insufficient to justify differences in biological behavior of the tumor. The present findings do not support a significant role of MCs in lymphangiogenesis.
RP20 - RADIOGRAPHIC FINDINGS IN PATIENTS WITH MEDICATION-RELATED OSTEONECROSIS OF THE JAW. CAMILA LOPES CARDOSO, MARCOS MARTINS CURI, CAROL ARRABAL BARROS, LUCIANA MARIA PAES DA SILVA RAMOS FERNANDES, CLÁUDIA CURRA, SOLANGE DE OLIVEIRA BRAGA FRANZOLIN, CARLOS CÉSAR DE ANTONI. Knowledge of imaging aspects of medication-related osteonecrosis of the jaw (MRONJ) represents valuable importance, since some cases may be asymptomatic. The aim of this study was to retrospectively assess the radiographic findings in patients with MRONJ. The study was conducted with records and panoramic radiographs of 35 patients treated with bisphosphonates and diagnosed with MRONJ. During radiographic analysis, findings such as osteolytic area, erosion of cortical bone, bone sequestration, bone sclerosis, periosteal reaction, abnormalities in the lamina dura, presence of pathologic fracture were surveyed. Medical information and staging were also recorded to correlate with radiographic findings. All panoramic radiographs showed important findings in the jaws. Osteosclerosis was the most frequent alteration, followed by osteolysis and abnormalities of the lamina dura. The mandible was more affected than maxilla. There was no significant difference between genders nor significant correlation between the number of injuries with age, bisphosphonate usage time, and staging of the disease. Panoramic radiograph is a useful screening tool in patients submitted to antiresorptive and antiangiogenic therapies, especially with bisphosphonates. In this study, although all cases had been previously diagnosed as MRONJ, radiographic findings may improve early diagnosis, establishing preventive approach for this complication. FAPESP 2014/13055-0.
RP21 - USE OF PLATELET-RICH PLASMA IN MEDICATION RELATED OSTEONECROSIS OF JAW TREATMENT: EXPERIMENTAL STUDY. CAMILA LOPES CARDOSO, CLÁUDIA CURRA, MARCOS MARTINS CURI, CAMILA DIONÍSIO ARGENTINO, OSNY FERREIRA JÚNIOR, SOLANGE DE OLIVEIRA BRAGA FRANZOLIN, ANDRÉIA APARECIDA DA SILVA. Bone resection associated with platelet-rich plasma (PRP) is a proposal in management of medication related osteonecrosis of jaw (MRONJ), which has demonstrated clinical success in the symptomatic cases. The aim of this study was to evaluate the surgical treatment of MRONJ using PRP. 30 rats received intravenous zoledronic acid (35mg/kg in 0.9% NaCl, for 8 weeks) and the upper right incisor extraction was performed to induce MRONJ. The surgical treatment was performed after the diagnosis of MRONJ. The experimental group (1) was submitted to marginal resection of the affected region, alveolar curettage and filling with PRP. The control group (2) was subjected to the same procedure, except the use of PRP. After 14, 28 and 42 days, the animals were euthanized and it was performed histomorphometric analysis, using Image-ProÒ Plus version 5.1.2 software, quantifying the following structures:
OOOO August 2017 newly bone formation (BO) connective tissue (CT), inflammatory infiltration (IF) and blood vessel (BV). Group 1 showed significantly greater amount of BO, CT and BV compared with group 2. There was no significant difference regarding the IF. From this study, it was concluded that the use of PRP showed better bone repair compared with the group without PRP. Financial support: FAPESP 2014/10693-6.
RP22 IMMUNOHISTOCHEMICAL ANALYSIS OF OCT-4 AND CD44 IN BENIGN EPITHELIAL ODONTOGENIC LESIONS. RAFAELLA BASTOS LEITE, EDUARDO ALONSO CRUZ MONROY, JOABE DOS SANTOS PEREIRA, MARCELO ANDERSON BARBOSA NASCIMENTO, ADALBERTO MOSQUEDA-TAYLOR, LEÃO PEREIRA PINTO, LÉLIA BATISTA DE SOUZA. The aim of this study was to identify epithelial cells expressing stem cell markers by immunoexpression of Oct-4 and CD44 in a series of cases of benign epithelial odontogenic lesions. The sample was comprised of 20 cases of odontogenic keratocyst (OKC), 20 solid/multicystic ameloblastoma and 20 adenomatoid odontogenic tumor (AOT). Expression of Oct-4 and CD44 was evaluated based on the percentage of positive cells and immunostaining intensity. The sum of these scores constituted in total score immunostaining, which varied from 0 to 7. All cases were positive and some demonstrated high expression for both markers. Analysis of Oct-4 expression revealed no statistically significant differences (P ¼ .406) among the studied lesions. Regarding the CD44 expression, there was a statistically significant difference between cases of ameloblastoma and AOT in relation to OKC, with the latter show more cases in score 7 (P ¼ .034). The results of this study identified the presence of cells with stemness characteristics arranged in various sites in the epithelial component of the studied lesions. Taken together, the findings suggested a possible role in histogenesis and differentiation in benign epithelial odontogenic lesions, thus contributing to the development of these lesions.
RP23 - IMMUNE CELL PROFILE OF DENTAL PULP IN RATS TREATED WITH ZOLEDRONIC ACID. PAULO GOBERLÂNIO DE BARROS SILVA, MARIA ELISA QUEZADO LIMA VERDE, ANTONIO ERNANDO CARLOS FERREIRA JUNIOR, CAMILA CARVALHO DE OLIVEIRA, FABRÍCIO BITÚ SOUSA, MÁRIO ROGÉRIO LIMA MOTA, ANA PAULA NEGREIROS NUNES ALVES. The objective of this study was to characterize the pulp immune cell profile in the teeth of rats treated with Zoledronic Acid (ZA). For this Male Wistar rats (n¼6) received 4 intravenous infusions of ZA (0.04, 0.20 or 1.00 mg/kg) or saline. In the 70th day, they were euthanized. The first right molar was microscopically examined (H&E, toluidine blue and immunohistochemistry for CD68, Tumor Necrosis Factor (TNF)-a, Interleukin(IL)-1b, Inducible Nitric Oxide Synthase (iNOS), Nuclear Factor kappa B (NFkB) and IL-18 binding protein (IL-18bp). Were analyzed the ectasic/dilated vessels, the inflammatory cells (mast cells and mononuclear CD68 positive cells) and intensity of immunostaining for inflammatory markers in odontoblasts and nonodontoblasts pulp cells. Were used the Kruskall-Wallis/Dunn’s and the chi-square tests (P<.05). There is no differences in number of animals exhibiting dilated/ectasia blood vessels (P¼.242), inflammatory cells (P¼.489) and in the number of
RP20 - RADIOGRAPHIC FINDINGS IN PATIENTS WITH MEDICATION-RELATED OSTEONECROSIS OF THE JAW. CAMILA LOPES CARDOSO, MARCOS MARTINS CURI, CAROL ARRABAL BARROS, LUCIANA MARIA PAES DA SILVA RAMOS FERNANDES, CLÁUDIA CURRA, SOLANGE DE OLIVEIRA BRAGA FRANZOLIN, CARLOS CÉSAR DE ANTONI. Knowledge of imaging aspects of medication-related osteonecrosis of the jaw (MRONJ) represents valuable importance, since some cases may be asymptomatic. The aim of this study was to retrospectively assess the radiographic findings in patients with MRONJ. The study was conducted with records and panoramic radiographs of 35 patients treated with bisphosphonates and diagnosed with MRONJ. During radiographic analysis, findings such as osteolytic area, erosion of cortical bone, bone sequestration, bone sclerosis, periosteal reaction, abnormalities in the lamina dura, presence of pathologic fracture were surveyed. Medical information and staging were also recorded to correlate with radiographic findings. All panoramic radiographs showed important findings in the jaws. Osteosclerosis was the most frequent alteration, followed by osteolysis and abnormalities of the lamina dura. The mandible was more affected than maxilla. There was no significant difference between genders nor significant correlation between the number of injuries with age, bisphosphonate usage time, and staging of the disease. Panoramic radiograph is a useful screening tool in patients submitted to antiresorptive and antiangiogenic therapies, especially with bisphosphonates. In this study, although all cases had been previously diagnosed as MRONJ, radiographic findings may improve early diagnosis, establishing preventive approach for this complication. FAPESP 2014/13055-0.
RP21 - USE OF PLATELET-RICH PLASMA IN MEDICATION RELATED OSTEONECROSIS OF JAW TREATMENT: EXPERIMENTAL STUDY. CAMILA LOPES CARDOSO, CLÁUDIA CURRA, MARCOS MARTINS CURI, CAMILA DIONÍSIO ARGENTINO, OSNY FERREIRA JÚNIOR, SOLANGE DE OLIVEIRA BRAGA FRANZOLIN, ANDRÉIA APARECIDA DA SILVA. Bone resection associated with platelet-rich plasma (PRP) is a proposal in management of medication related osteonecrosis of jaw (MRONJ), which has demonstrated clinical success in the symptomatic cases. The aim of this study was to evaluate the surgical treatment of MRONJ using PRP. 30 rats received intravenous zoledronic acid (35mg/kg in 0.9% NaCl, for 8 weeks) and the upper right incisor extraction was performed to induce MRONJ. The surgical treatment was performed after the diagnosis of MRONJ. The experimental group (1) was submitted to marginal resection of the affected region, alveolar curettage and filling with PRP. The control group (2) was subjected to the same procedure, except the use of PRP. After 14, 28 and 42 days, the animals were euthanized and it was performed histomorphometric analysis, using Image-ProÒ Plus version 5.1.2 software, quantifying the following structures:
OOOO August 2017 newly bone formation (BO) connective tissue (CT), inflammatory infiltration (IF) and blood vessel (BV). Group 1 showed significantly greater amount of BO, CT and BV compared with group 2. There was no significant difference regarding the IF. From this study, it was concluded that the use of PRP showed better bone repair compared with the group without PRP. Financial support: FAPESP 2014/10693-6.
RP22 IMMUNOHISTOCHEMICAL ANALYSIS OF OCT-4 AND CD44 IN BENIGN EPITHELIAL ODONTOGENIC LESIONS. RAFAELLA BASTOS LEITE, EDUARDO ALONSO CRUZ MONROY, JOABE DOS SANTOS PEREIRA, MARCELO ANDERSON BARBOSA NASCIMENTO, ADALBERTO MOSQUEDA-TAYLOR, LEÃO PEREIRA PINTO, LÉLIA BATISTA DE SOUZA. The aim of this study was to identify epithelial cells expressing stem cell markers by immunoexpression of Oct-4 and CD44 in a series of cases of benign epithelial odontogenic lesions. The sample was comprised of 20 cases of odontogenic keratocyst (OKC), 20 solid/multicystic ameloblastoma and 20 adenomatoid odontogenic tumor (AOT). Expression of Oct-4 and CD44 was evaluated based on the percentage of positive cells and immunostaining intensity. The sum of these scores constituted in total score immunostaining, which varied from 0 to 7. All cases were positive and some demonstrated high expression for both markers. Analysis of Oct-4 expression revealed no statistically significant differences (P ¼ .406) among the studied lesions. Regarding the CD44 expression, there was a statistically significant difference between cases of ameloblastoma and AOT in relation to OKC, with the latter show more cases in score 7 (P ¼ .034). The results of this study identified the presence of cells with stemness characteristics arranged in various sites in the epithelial component of the studied lesions. Taken together, the findings suggested a possible role in histogenesis and differentiation in benign epithelial odontogenic lesions, thus contributing to the development of these lesions.
RP23 - IMMUNE CELL PROFILE OF DENTAL PULP IN RATS TREATED WITH ZOLEDRONIC ACID. PAULO GOBERLÂNIO DE BARROS SILVA, MARIA ELISA QUEZADO LIMA VERDE, ANTONIO ERNANDO CARLOS FERREIRA JUNIOR, CAMILA CARVALHO DE OLIVEIRA, FABRÍCIO BITÚ SOUSA, MÁRIO ROGÉRIO LIMA MOTA, ANA PAULA NEGREIROS NUNES ALVES. The objective of this study was to characterize the pulp immune cell profile in the teeth of rats treated with Zoledronic Acid (ZA). For this Male Wistar rats (n¼6) received 4 intravenous infusions of ZA (0.04, 0.20 or 1.00 mg/kg) or saline. In the 70th day, they were euthanized. The first right molar was microscopically examined (H&E, toluidine blue and immunohistochemistry for CD68, Tumor Necrosis Factor (TNF)-a, Interleukin(IL)-1b, Inducible Nitric Oxide Synthase (iNOS), Nuclear Factor kappa B (NFkB) and IL-18 binding protein (IL-18bp). Were analyzed the ectasic/dilated vessels, the inflammatory cells (mast cells and mononuclear CD68 positive cells) and intensity of immunostaining for inflammatory markers in odontoblasts and nonodontoblasts pulp cells. Were used the Kruskall-Wallis/Dunn’s and the chi-square tests (P<.05). There is no differences in number of animals exhibiting dilated/ectasia blood vessels (P¼.242), inflammatory cells (P¼.489) and in the number of