Raman scattering spectra of panax ginseng chloroplasts and living leaves

Journal of Luminescence 40&41 (1988) 223 224 North-Holland, Amsterdam

223

RAMAN SCATIERING SPECTRA OF PANAX GINSENG CHLOROPLASTS AND LIVING LEAVES

shuyan TPNG, Lixia LIU and Jingxiu WAf~ Changchun Institute of Physics, Academia Sinica, China

We first studied the quasi—resonance Raman scattering of Giseng LHA in living leaves. Possible molecular arrangment of LHA was proposed.

1. INTRODUCTION The plant pigments can be classified into

As Raman spectral intensity is indirectly proportional to tetrasquare of scattering fre—

light harvesting antenna pigments(LHA) and reac—

qency,

tion

laser

center

roles

in

pigments(RC)

photosynthesis.

quasi—resonance

Raman

according In

this

scattering

to

their

paper,

the specimen can waves at

457.9nm,

be excited with the 488.Onm and 632.8nm

the

wavelength according to the character absorp—

of

tion bands of Ginseng pigments. The results are

spectra

Ginseng (Panax Ginseng) LHA in living leaves were

given in Fig. 1.

first advanced and comparisions were made with I (a,u)

those of Ginseng chloroplasts. Vibrational modes spectral lines

originated from different

con-

jugated groups, molecular chains and conjugated plants of LHA were researched. With the select stimulating of different laser wavelength, different patterns of molecule linkages of 3 =

—

c

0 groups and 9—keto carbonyl groups in their

632 8~

/

/

molecules were studied. The possible pattern of molecular arrangment of Ginseng LHA was propos— ed. This is an important step in the photosyn—

1500

1000 v ----

500

thetic process in high plant and algae. It determines the ability and nature of the plant in which the energy is absorbed from sunlight and transported to the reaction center.

FIQJRE 1 Raman scattering spectra of Ginseng chioroplasts under different exciting conditions From the Raman spectra of Ginseng chloroplast

2. MATERIALS AND METHODS The Ginseng

chloroplasts were

under different exciting conditions, prepareed

by

Arnon methods. The Resonace Raman Spectra were measured 488.Oiurl

with TY—T800, excited with 457.9nm, and 632.8nm laser wavelength.

3. RESULTS AND DISCUSSION

three ac—

tive vibration regions can be divided: (a)

Active

carbonyl region

of

chlorophyll

(1550 cm’—— 3750 cm’). Figure 1 shows the spec tral bands at 1565cm’, 158Ocm~, 16O5LIII1, 163Oc m’, 1645cm’, 1700cm’, 172Ocm~, etc..

1675c m~,1695cm~, Among them, the bands

3.1 The Raman spectra of Ginseng chloroplast

at 1630cm’, 1645cm’ and 1695cm~ are resulted

under differnt excitation wavelengths

from

0022 2313/88/$03.50 © Elsevier Science Publishers B.V. (North-Holland Physics Publishing Division)

the drawing

and stretching

of 3

—

C

=

0

224

S. Tang et al.

groups

and

belong

chlorophyll

b.

to

But

the

the

bands

others

/

Panax Ginseng chioroplasts and living leaves

of antenna are

resulted

from the 9—keto carbonyl groups and belong to bands of whole antenna molecules. (b) The conjugated plane active region of prophyrin marcroring (400cm_1_1550cm~). Froa denser

this

stretching

spectral

vibration

groups,

bands

formed

the

or C~~LC (1505cm 1, 1530cm’,

1565cm’) bands of C~-~-~-N (1100cm~,l60Ucm~,

500

V

1000

1500

—1 cm

1190cm~, 1220cm~) and bands with some charac ters of hydrate ploymers (1450cm~,1350c’r~,1280 cm’,

1005cm~, 960cm~, 850cm~,770an~720cm’,

665cm~, 615 cm —1

560cm’,

495cm~, 475cm~) living leaves under room tenperature.

etc. can be distinguished. (c) Lower the

streching

FIGURE 2 Raman scattering spectra of Ginseng chioroplasts (1) and living leaves (2)

freqencies

(200cm ‘—400cm 1) from

movement

of

N

4—Mg

bands.

Raman lines of N4—Mg bands at 320cm’,

The

355cm~,

370cm~ were determined

to see that the

plasts can also be seen in Raman spectra of liv— ing leaves.

But in lower frequendes region more

Raman lines

Analying the above Raman spectra in three region and comparing the spectra of different assembled

It is easy

Raman lines of Ginseng chioro—

chloroplasts.

appear

for li~ng leaves

than for

The pollymeric state of Ginseng LHA

may be hydrate polymerized.

states (monomer, di.mer and oligomer) of vitro chlorophyll,

the spectra

of L H A

molecules

composition

and

distribution

REFERENCES

of Ginseng chioroplasts are si-

milar to those of the hydrate plymers. 3.2 The Raman scattering spectra

~ M. Lutz, Biochm.Biophy. 2.

living leaves Figure 2 shows the

Acta. (1977) 460.

of Ginseng

Raman spectra fo Ginseng

N. Lutz and J.S. Brown, Chl. Organization and Energy Transfer in Photosynthesis (1979) pp. 105—121.

3. E.P. Naheev, etc. Biophysics (1986) XXX. 2. 183