- Email: [email protected]
Rapid method for determination of plasma fibrinogen
404
Selected abstracts
*Adamis, D. ~f.: Rapid Method for Dctcnnina· tion of Plasma Fibrinogen, p. J 07. Lostroh: In Vitro Response of Mouse T cstis to Human Chorionic Gonadotropin, p. 25. Studies were undertaken to determine the growth and rc.>ponsc of mott'C' testis in in vitro culture media. Te.,tes from mice :1 days of age ln'n! culmrt'd in an in vitro system fnr 7 tn l4 days on described culture media ..\fter 7 days of culture, tubular size drcrcaSL'd and interstitial cell cytoplasm decreased. These ckgerwrati\'(' changes were amdioratcd with the addition nf IICG to tlw culture media. The addition of HCG enhanced rhe secretion of androg-en for 11 days nml•·r in vitro t'<>nditioll'.
Herrmann, Schindl, and Bondy: Effed of Estrogen on Steroid I.evels in Plasma and Urine, p. l 03. The present study investigated the e!I<~cts of physiologic lewis of naturally occurring estr<)· genic material on the adrenal steroid levels in plasma and urine and abo determined :\CTH re~pomivcltl'>S mHkr these conditinus. The experiment rc,·ealed that le,·el~ of hvdrncortisone remained tmch:uu;:ed J ftn cstrng:•n administration and there w:1s no sir;nilicant difference in n·spou::c to AC:TH ,,·ith ''r \\ith(>\it estrogen. There was a decn~a,,·d urinan· r<'\1· ing Ic,·el of till~ 17-hydroxy st,•roids after .·strngen administration and the rcspons•• to :\CTH plus estrogen wa~ less than t•J ACTH alone. Urinary pregnanctriol excrNion was diminished after estrogen administratiott and levels were lower after ACTH plu~ estrogen than with ACTH alone. It has been shown that while the administration of estrogen did not affect the plasma levels of adrenal steroids nor their response to ACTH infusions, the excretion of adrenal steroids is significantly decreased. The authors feel that although this may represent iutt~rfercnce with the biosynthesis of these pmdw t9 by estrogen, a more attractive theory is that of a de· layed catabolism brought about by increased protein-binding as occurs with hydrocortisone. Lawrence f. Sanders Adamis: Rapid Method for Determination of Plasma Fibrinogen, p. 107. A technique to determine the level of plasma fibrinogen rapidly and without loss of accuracy
:\m.
J.
August. !'ltil! Obst. & r;yn<·C,
is presentt·d. The method outlined involves: '. I ) the preparation of a ,ubstratc reagent of defibrinated plasma which may be stored indefi-
nitely, :::; the preparation of plasma tu b,; tested in lmtfr~red dilutinns. the test utilizing the plasma t< > lw it's ted, substrate reagen1, CaCI~. and rahbit brain thromboplastin "·hi,·h gives clotting t.irne in seconds, the \omparison of the determined dotting time in second! with an accurah· (\raph which correlates dot· ting times with plasma Jibrinogen p(~n·c·ntai.{C lr:vds.
Wiener klinische Wochenschrift rrol. 21, Oct. :!8, 1959. "'IIusslein, H., zmd Hofhaml. W.: The Po.;,;iIJi!itics of Artificial Dcbv in Menstruatiou. p. 3:.!1.
Husslein and Hofhansl: Possibilities of Artificial Delay in Menstruation, p. 821. Artificial. phanltacologically induced delav in menstruation 11 a.; produn~d in a tot:t! of R6 patient,; with 0 rwwly available oral estrogen, cthylnilnortcstostcron acrtate in mmbination with •·thinyl cstradiol ;md hvdroxyprogcsterone caprnnate. TIH· indication> for d<'laying nwnstrua'· inn inc Inded po h m<·norrlwi c .;vnd ronws to,e;et!tr·r with •·kcl i\ l' delays based on the wi-;h of tlw pati•··nts, "'lll<' ,·,f "horn wished to b·~ fn·~~ from nwnstruation during or y;u:ations. The .tutlwrs' data .;lww that when tlw Jll<'HSlrttal cotdd lw :l•Ttiratdv predicted in ach·anc<', delay in nwHstruation can bt~ induced by tlw administr:t1 inn of appropriate combinations of cstrog•·n, a11dror;•·n and progt·st<:roJw \\·ithout any danger to the p::llit'nt. The orally administered !'lhinyl estradiol was a mf'thod which the :lllthors preferred to the injection of '''tr;tdiol lwnznate in cnmhillation with hydroxvprogcstnone capronate, requirinl( a much lower dosage and producing a much more predirtahl<: dTect. Douglas At. Haynes
Zentralblatt fur Gynakologie Vol. 81, July 11, 1959. Fuhrmann, K.: Colorimetric Dctcrmina tion and Histochemical Demonstration of Amino Peptidase in Tissues of the Female Genital Tract, p. 1105. Aldca, A., Luca, V., and Filipescu, L: Question on Pathogenesis and Therapy of Amenorrhea and Genital Tuberculosis, p. 1123.
Selected abstracts
*Adamis, D. ~f.: Rapid Method for Dctcnnina· tion of Plasma Fibrinogen, p. J 07. Lostroh: In Vitro Response of Mouse T cstis to Human Chorionic Gonadotropin, p. 25. Studies were undertaken to determine the growth and rc.>ponsc of mott'C' testis in in vitro culture media. Te.,tes from mice :1 days of age ln'n! culmrt'd in an in vitro system fnr 7 tn l4 days on described culture media ..\fter 7 days of culture, tubular size drcrcaSL'd and interstitial cell cytoplasm decreased. These ckgerwrati\'(' changes were amdioratcd with the addition nf IICG to tlw culture media. The addition of HCG enhanced rhe secretion of androg-en for 11 days nml•·r in vitro t'<>nditioll'.
Herrmann, Schindl, and Bondy: Effed of Estrogen on Steroid I.evels in Plasma and Urine, p. l 03. The present study investigated the e!I<~cts of physiologic lewis of naturally occurring estr<)· genic material on the adrenal steroid levels in plasma and urine and abo determined :\CTH re~pomivcltl'>S mHkr these conditinus. The experiment rc,·ealed that le,·el~ of hvdrncortisone remained tmch:uu;:ed J ftn cstrng:•n administration and there w:1s no sir;nilicant difference in n·spou::c to AC:TH ,,·ith ''r \\ith(>\it estrogen. There was a decn~a,,·d urinan· r<'\1· ing Ic,·el of till~ 17-hydroxy st,•roids after .·strngen administration and the rcspons•• to :\CTH plus estrogen wa~ less than t•J ACTH alone. Urinary pregnanctriol excrNion was diminished after estrogen administratiott and levels were lower after ACTH plu~ estrogen than with ACTH alone. It has been shown that while the administration of estrogen did not affect the plasma levels of adrenal steroids nor their response to ACTH infusions, the excretion of adrenal steroids is significantly decreased. The authors feel that although this may represent iutt~rfercnce with the biosynthesis of these pmdw t9 by estrogen, a more attractive theory is that of a de· layed catabolism brought about by increased protein-binding as occurs with hydrocortisone. Lawrence f. Sanders Adamis: Rapid Method for Determination of Plasma Fibrinogen, p. 107. A technique to determine the level of plasma fibrinogen rapidly and without loss of accuracy
:\m.
J.
August. !'ltil! Obst. & r;yn<·C,
is presentt·d. The method outlined involves: '. I ) the preparation of a ,ubstratc reagent of defibrinated plasma which may be stored indefi-
nitely, :::; the preparation of plasma tu b,; tested in lmtfr~red dilutinns. the test utilizing the plasma t< > lw it's ted, substrate reagen1, CaCI~. and rahbit brain thromboplastin "·hi,·h gives clotting t.irne in seconds, the \omparison of the determined dotting time in second! with an accurah· (\raph which correlates dot· ting times with plasma Jibrinogen p(~n·c·ntai.{C lr:vds.
Wiener klinische Wochenschrift rrol. 21, Oct. :!8, 1959. "'IIusslein, H., zmd Hofhaml. W.: The Po.;,;iIJi!itics of Artificial Dcbv in Menstruatiou. p. 3:.!1.
Husslein and Hofhansl: Possibilities of Artificial Delay in Menstruation, p. 821. Artificial. phanltacologically induced delav in menstruation 11 a.; produn~d in a tot:t! of R6 patient,; with 0 rwwly available oral estrogen, cthylnilnortcstostcron acrtate in mmbination with •·thinyl cstradiol ;md hvdroxyprogcsterone caprnnate. TIH· indication> for d<'laying nwnstrua'· inn inc Inded po h m<·norrlwi c .;vnd ronws to,e;et!tr·r with •·kcl i\ l' delays based on the wi-;h of tlw pati•··nts, "'lll<' ,·,f "horn wished to b·~ fn·~~ from nwnstruation during or y;u:ations. The .tutlwrs' data .;lww that when tlw Jll<'HSlrttal cotdd lw :l•Ttiratdv predicted in ach·anc<', delay in nwHstruation can bt~ induced by tlw administr:t1 inn of appropriate combinations of cstrog•·n, a11dror;•·n and progt·st<:roJw \\·ithout any danger to the p::llit'nt. The orally administered !'lhinyl estradiol was a mf'thod which the :lllthors preferred to the injection of '''tr;tdiol lwnznate in cnmhillation with hydroxvprogcstnone capronate, requirinl( a much lower dosage and producing a much more predirtahl<: dTect. Douglas At. Haynes
Zentralblatt fur Gynakologie Vol. 81, July 11, 1959. Fuhrmann, K.: Colorimetric Dctcrmina tion and Histochemical Demonstration of Amino Peptidase in Tissues of the Female Genital Tract, p. 1105. Aldca, A., Luca, V., and Filipescu, L: Question on Pathogenesis and Therapy of Amenorrhea and Genital Tuberculosis, p. 1123.