- Email: [email protected]
Reactive oxygen species and antioxidant response of catalase in Helianthus annuus L. subjected to Cd+2 stress
Abstracts / Journal of Biotechnology 161S (2012) 19–48
45
analyses for identification of ploidy levels and molecular markers (AFLP, RAPD and chloroplast trnL-trnF region genome-specific markers) to construct an association map at genome-wide level. These multidisciplinary studies at morphological and molecular level will be used for resolving taxonomic confusion of Colchicum L. genus.
levels were observed for the Hsp gene, encoding heat shock protein responsible for the re-establishing of normal protein conformation under stress conditions.
http://dx.doi.org/10.1016/j.jbiotec.2012.07.144
Reactive oxygen species and antioxidant response of catalase in Helianthus annuus L. subjected to Cd+2 stress
Assessing genetic variability in winter wheat cultivars using microsatellite markers Funda
Senturk-Akfirat 1,∗ ,
Ahu Altinkut
Uncuoglu 2
1
Department of Molecular Biology and Genetics, Gebze Institute of Technology, Cayirova Campus, Gebze, Kocaeli 41700, Turkey 2 Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe, Istanbul 34722,Turkey E-mail address: [email protected] (F. Senturk-Akfirat). Genetic diversity is the ultimate basis for genetic improvement. The knowledge with germplasms is critical in the improvement of crops. Genetic diversity of seven winter hexaploid wheat (Triticum aestivum L.) genotypes resistant and susceptible to yellow rust disease was assessed by 223 microsatellite markers. These genotypes were used as sources of DNA for PCR amplifications. A total of 741 allelic variants were detected at 216 microsatellite loci, ranging from one to eighteen per locus with an average of 3.09. A relatively high level (66.45%) of polymorphism was observed with 216 microsatellite primers among the seven wheat genotypes. Genetic similarities calculated from microsatellite data between genotypes by a neighbour joining algorithm. The polymorphic information content (PIC) values of the loci ranged from 0.21 (Xgwm182) to 0.93 (Xgwm642). PI178383 was the most differentiated of the seven genotypes. The highest similarity was 0.633 between Altay2000 and ES14. The lowest similarity was 0.479 between Sonmez2001 and PI178383. http://dx.doi.org/10.1016/j.jbiotec.2012.07.145
http://dx.doi.org/10.1016/j.jbiotec.2012.07.146
I˙ lker Buyuk, Ayla Behnejad Kazancik ∗ , Sumer Aras Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey E-mail address: [email protected] (A.B. Kazancik). Cadmium is a widespread environmental pollutant which can be assimilated by plants in contaminated soils. It is a highly soluble heavy metal thereby it can easily effect physiologic and genetic integrity of plants through with overproduction of reactive oxygen species (ROS). However there is less information about the effects of Cd+2 on sunflowers plant which is an important trade plant. In this regard, we evaluated Cd+2 effects on lipid peroxidation (MDA), total soluble protein level, catalase enzyme activity and catalase mRNA level (CATA3) using quantitative real time PCR technique. We observed increments in MDA levels and catalase enzyme activity at all concentrations of treatments. Especially, 240 mg l−1 of Cd+2 treatment lead to highest MDA level and catalase enzyme activity while all concentrations revealed decreased total soluble protein levels. Differential expression patterns of catalase gene depend on metal concentrations and our results showed the efficiency of the enzyme directly on scavenging ROS. http://dx.doi.org/10.1016/j.jbiotec.2012.07.147
Relationship between hyperxydricidy and oxidative stress in sugarbeet tissue culture Ayse Sen ∗ , Sema Alikamanoglu Department of Biology, Faculty of Science, Istanbul, Istanbul University, 34459, Turkey
Relation between different concentrations of Cd+2 treatment and mRNA levels of 17.7 kDa heat-shock protein gene in Helianthus annuus plants I˙ lker Buyuk, Asli Fazlioglu ∗ , Sumer Aras Department of Biology, Faculty of Science, Ankara University, Ankara,Turkey E-mail address: [email protected] (A. Fazlioglu). Expression analyses of gene sets which are involved in response to stress are important to evaluate meaningful physiological and biochemical effects of different metals. Each heavy metal cause accumulation of reactive oxygen species above allowable levels and cause some characteristic damages that depends on the concentrations of heavy metals and plant species. In this study, we investigated the effects of different concentrations of Cd+2 on root length, dry weight, MDA level and expression levels of 17.7 kDa heat-shock protein gene (Hsp) using quantitative real time PCR technique. MDA levels were increased significantly upon exposure to all concentrations of Cd+2 and these increments reflect the stress effects of Cd+2 treatments on sunflower plants. Root lengths were decreased as expected at all concentrations of Cd+2 treatments while dry weights were increased. Differential expression
E-mail address: [email protected] (A. Sen). Hyperhydricity is a frequent problem in tissue culture, which is limiting the in vitro growth and multiplication. It is commonly defined that it is a morphological, anatomical and physiological abnormalities that they make the plant tissue water-swollen. In this study, we aimed to investigate possible relationship between hyperhydricity and oxidative stress. Upon this purpose, sugarbeet (Beta vulgaris L. cv. Felicita) shoot tip explants were planted into MS medium involved with 1 mg l−1 MS vitamin, 1 mg l−1 BAP, 30 g l−1 sucrose, various concentration of PEG6000 (0–80 g l−1 ) and 10 g l−1 agar. During the 4 weeks, depending on the increasing application concentrations of PEG6000 and time, increasing levels of hyperhydration and darkness were observed in culture. On the 28th day, the antioxidant enzymes activities (SOD, CAT, APX, and POX) and malondialdehyde content were measured. Afterwards, all results were statistically evaluated and compared with each other. In comparison with the control, activities of SOD, CAT, APX, and POX in the hyperhydric shoots increased especially at lower concentrations of PEG6000 and the malondialdehyde content also enhanced under different PEG6000 treatments. http://dx.doi.org/10.1016/j.jbiotec.2012.07.148
45
analyses for identification of ploidy levels and molecular markers (AFLP, RAPD and chloroplast trnL-trnF region genome-specific markers) to construct an association map at genome-wide level. These multidisciplinary studies at morphological and molecular level will be used for resolving taxonomic confusion of Colchicum L. genus.
levels were observed for the Hsp gene, encoding heat shock protein responsible for the re-establishing of normal protein conformation under stress conditions.
http://dx.doi.org/10.1016/j.jbiotec.2012.07.144
Reactive oxygen species and antioxidant response of catalase in Helianthus annuus L. subjected to Cd+2 stress
Assessing genetic variability in winter wheat cultivars using microsatellite markers Funda
Senturk-Akfirat 1,∗ ,
Ahu Altinkut
Uncuoglu 2
1
Department of Molecular Biology and Genetics, Gebze Institute of Technology, Cayirova Campus, Gebze, Kocaeli 41700, Turkey 2 Department of Bioengineering, Faculty of Engineering, Marmara University, Goztepe, Istanbul 34722,Turkey E-mail address: [email protected] (F. Senturk-Akfirat). Genetic diversity is the ultimate basis for genetic improvement. The knowledge with germplasms is critical in the improvement of crops. Genetic diversity of seven winter hexaploid wheat (Triticum aestivum L.) genotypes resistant and susceptible to yellow rust disease was assessed by 223 microsatellite markers. These genotypes were used as sources of DNA for PCR amplifications. A total of 741 allelic variants were detected at 216 microsatellite loci, ranging from one to eighteen per locus with an average of 3.09. A relatively high level (66.45%) of polymorphism was observed with 216 microsatellite primers among the seven wheat genotypes. Genetic similarities calculated from microsatellite data between genotypes by a neighbour joining algorithm. The polymorphic information content (PIC) values of the loci ranged from 0.21 (Xgwm182) to 0.93 (Xgwm642). PI178383 was the most differentiated of the seven genotypes. The highest similarity was 0.633 between Altay2000 and ES14. The lowest similarity was 0.479 between Sonmez2001 and PI178383. http://dx.doi.org/10.1016/j.jbiotec.2012.07.145
http://dx.doi.org/10.1016/j.jbiotec.2012.07.146
I˙ lker Buyuk, Ayla Behnejad Kazancik ∗ , Sumer Aras Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey E-mail address: [email protected] (A.B. Kazancik). Cadmium is a widespread environmental pollutant which can be assimilated by plants in contaminated soils. It is a highly soluble heavy metal thereby it can easily effect physiologic and genetic integrity of plants through with overproduction of reactive oxygen species (ROS). However there is less information about the effects of Cd+2 on sunflowers plant which is an important trade plant. In this regard, we evaluated Cd+2 effects on lipid peroxidation (MDA), total soluble protein level, catalase enzyme activity and catalase mRNA level (CATA3) using quantitative real time PCR technique. We observed increments in MDA levels and catalase enzyme activity at all concentrations of treatments. Especially, 240 mg l−1 of Cd+2 treatment lead to highest MDA level and catalase enzyme activity while all concentrations revealed decreased total soluble protein levels. Differential expression patterns of catalase gene depend on metal concentrations and our results showed the efficiency of the enzyme directly on scavenging ROS. http://dx.doi.org/10.1016/j.jbiotec.2012.07.147
Relationship between hyperxydricidy and oxidative stress in sugarbeet tissue culture Ayse Sen ∗ , Sema Alikamanoglu Department of Biology, Faculty of Science, Istanbul, Istanbul University, 34459, Turkey
Relation between different concentrations of Cd+2 treatment and mRNA levels of 17.7 kDa heat-shock protein gene in Helianthus annuus plants I˙ lker Buyuk, Asli Fazlioglu ∗ , Sumer Aras Department of Biology, Faculty of Science, Ankara University, Ankara,Turkey E-mail address: [email protected] (A. Fazlioglu). Expression analyses of gene sets which are involved in response to stress are important to evaluate meaningful physiological and biochemical effects of different metals. Each heavy metal cause accumulation of reactive oxygen species above allowable levels and cause some characteristic damages that depends on the concentrations of heavy metals and plant species. In this study, we investigated the effects of different concentrations of Cd+2 on root length, dry weight, MDA level and expression levels of 17.7 kDa heat-shock protein gene (Hsp) using quantitative real time PCR technique. MDA levels were increased significantly upon exposure to all concentrations of Cd+2 and these increments reflect the stress effects of Cd+2 treatments on sunflower plants. Root lengths were decreased as expected at all concentrations of Cd+2 treatments while dry weights were increased. Differential expression
E-mail address: [email protected] (A. Sen). Hyperhydricity is a frequent problem in tissue culture, which is limiting the in vitro growth and multiplication. It is commonly defined that it is a morphological, anatomical and physiological abnormalities that they make the plant tissue water-swollen. In this study, we aimed to investigate possible relationship between hyperhydricity and oxidative stress. Upon this purpose, sugarbeet (Beta vulgaris L. cv. Felicita) shoot tip explants were planted into MS medium involved with 1 mg l−1 MS vitamin, 1 mg l−1 BAP, 30 g l−1 sucrose, various concentration of PEG6000 (0–80 g l−1 ) and 10 g l−1 agar. During the 4 weeks, depending on the increasing application concentrations of PEG6000 and time, increasing levels of hyperhydration and darkness were observed in culture. On the 28th day, the antioxidant enzymes activities (SOD, CAT, APX, and POX) and malondialdehyde content were measured. Afterwards, all results were statistically evaluated and compared with each other. In comparison with the control, activities of SOD, CAT, APX, and POX in the hyperhydric shoots increased especially at lower concentrations of PEG6000 and the malondialdehyde content also enhanced under different PEG6000 treatments. http://dx.doi.org/10.1016/j.jbiotec.2012.07.148