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Receptor signaling and cell proliferation
research
news
Thomas Htibschmann, Hirokazu Kobayashi and Gerhard Link for preprints. Research cited from my lab was supported by the National Science Foundation. Pal hlaliga Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854-8010, USA (tel +l 732 445 5329; fax +l 732 445 5735; e-mail maligaQmbcl.rutgers.edu) References 1 Gray, M.W. (1993) Origin and evolution of organelle genomes, Curr. Opin. Gener. Dev. 3, 88&890 2 Weihe, A., Hedtke. B. and Bomer, T. (1997) Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album. Nucleic Acids Res. 25, 2319-2325 3 Allison. L.A., Simon. L.D. and Ma&a, P. (1996) Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants, EMBO .I. 15,2802-2809 4 Hajdukiewicz, P., Allison, L.A. and Maliga, P. (1997) The two plastid RNA polymerases encoded by the nuclear and plastid compartments transcribe distinct groups of genes in tobacco plastids, EMBO .I. 16, 40414048
5 Hedtke, B., Bomer. T. and Weihe. A. (1997) Mitochondrial and chloroplast phage-type RNA polymerases in Arabidopsis. Science 217, X09-8 11 6 Pfannschmidt, T. and Link, G. The A and B forms of plastid DNA-dependent RNA polymerase from mustard (Sinapis alba L.) transcribe the same genes in a different developmental context, Mol. Gen. Genrr. (in press) 7 Link, G. (1996) Green life: control of chloroplast gene transcription, BioEssays 18,465471 8 Tanaka, K. et al. (I 997) Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arubidopsis rhalianu: evidence for the sigma factor heterogeneity in higher plant plastids, FEBS Len. 4 13. 309-3 I3 9 Isono, K. et al. Leaf-specifically expressed genes for polypeptides destined for chloroplasts with domains of sigma-70 factors of bacterial RNA polymerases in Arabidopsir thulium, Proc. N&l. Acad. Sci. L’. S. A. (in press) 10 Iratni, R. et al. (1997) Organ-specific transcription of the rm operon in spinach plastids, J. Biol. Chern. 272. 13676-13682 11 Baginsky. S., Tiller, K. and Link, G. (1997) Transcription factor phosphorylation by a protein kinase associated with chloroplast RNA polymerase from mustard (Sinapis &ha). Plant Mol. Biol. 34. 181-189
literature
12 Morden, C.W. et al. (1991) Plastid translation and transcription genes in a non-photosynthetic plant: intact. missing and pseudo genes, EMBO .I. IO. 3281-3288 13 Hess, W.R. ef al. (1993) Chloroplast rpsl5 and the rpoB/CI/CZ gene cluster are strongly transcribed in ribosome-deficient plastids: evidence for a functioning non-chloroplastencoded RNA polymerase, EMBO J. 12.563-571 14 Han, CD. er al. (1993) Aberrations in plastid transcripts and deficiency of plastid DNA in striped and albino mutants in maize, Planra 19 I, 552-563 15 Htibschmann. T. and Bomer, T. Characterization of transcription initiation sites in ribosomedeficient barley plastids. Plant Mol. Biol. (in preai) 16 Kapoor, S.. Suzuki, J.Y. and Sugiura. M. ( 1997) Identification and functional significance of a new class of non-consensus-type plastid promoters. Piunr .I. 1 I, 327-337 17 Lerbs-Mache. S. (1993) The I 10.kDa polypeptide of spinach plastid DNA-dependent RNA polymerase: single-subunit enzyme or catalytic core of multimeric enzyme complexes? Proc. Nd. Acad. Sci. U. S. A. 90, 5509-5513 18 DuBell, A.N. and Mullet, J.E. (1995) Differential transcription of pea chloroplast genes during lightinduced leaf development, Plant Phyiol. 109. 105-l 12
focus
Receptor signaling and cell proliferation Williams,
R.W., Wilson, J.M. and Meyerowitz, E.M. (1997) A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATAl signaling pathway, Proc. Nat/. Acad. Sci. U. S. A. 94,10467-10472
Biochemical assaysrevealed that the aphenotypesimilarto thatproducedby weak In Arabidopsis, mutationsin CLAVATAl kinase domainof CLVl autophosphorylatesclvl alleles.Thebasisof this wasexamined (CLVl) give riseto plantswith muchlarger but that autophosphoryl- usingafilter-bindingassay:fusionconstructs apicalandfloral meristems, suggesting that on serineresidues, CLVl actsto represscell proliferation.The ation is greatly reducedby the clvl -I mu- of KAPP andCLVl werefound to interact, sequence of CLVl appears to encodeamem- tation.Whenan inactiveform of the kinase andtheextentof thisinteractionwasdependstatusof CLVI. brane-bound receptorkinase,comprisingan wasincubatedwith theCLV 1kinase,thistoo ent on the phosphorylation revealingthatCLVl can Furthermore,the CLVl kinaseis able to N-terminal signal sequence;a seriesof wasphosphotylated, catalysethe phosphorylationof KAPP, and Theexpression of CLVl leucine-richrepeats;a membrane-spanningtransphosphorylate. CLVl. domain;andacytoplasmickinasedomain.In RNA is primarilyconfinedto the subepider- in turn KAPP can dephosphorylate There Thus,thedataindicatethatKAPP negatively an attempt to dissectthe CLVl signaling malcellsof apicalandfloralmeristems. pathway, Meyerowitz’s grouphave exam- is a similar.but moreextensive,patternof regulatesCLVl signaling,but its expression protein patternandability to interactwith otherrecepined the kinaseactivity of CLVl and its expressionof a kinase-associated suggests that it mayalsoplay ability to interact with other regulatory phosphatase(KAPP). Overexpressionof tor-likekinases a role in other transduction pathways. KAPP cDNA in transgenic plants gave rise to components.
6
January 1998, Vol. 3, No. 1
Copyright
0 1998 Elsevier Science Ltd. All rights reserved. 1360 - 1385/98/$19.00
news
Thomas Htibschmann, Hirokazu Kobayashi and Gerhard Link for preprints. Research cited from my lab was supported by the National Science Foundation. Pal hlaliga Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854-8010, USA (tel +l 732 445 5329; fax +l 732 445 5735; e-mail maligaQmbcl.rutgers.edu) References 1 Gray, M.W. (1993) Origin and evolution of organelle genomes, Curr. Opin. Gener. Dev. 3, 88&890 2 Weihe, A., Hedtke. B. and Bomer, T. (1997) Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album. Nucleic Acids Res. 25, 2319-2325 3 Allison. L.A., Simon. L.D. and Ma&a, P. (1996) Deletion of rpoB reveals a second distinct transcription system in plastids of higher plants, EMBO .I. 15,2802-2809 4 Hajdukiewicz, P., Allison, L.A. and Maliga, P. (1997) The two plastid RNA polymerases encoded by the nuclear and plastid compartments transcribe distinct groups of genes in tobacco plastids, EMBO .I. 16, 40414048
5 Hedtke, B., Bomer. T. and Weihe. A. (1997) Mitochondrial and chloroplast phage-type RNA polymerases in Arabidopsis. Science 217, X09-8 11 6 Pfannschmidt, T. and Link, G. The A and B forms of plastid DNA-dependent RNA polymerase from mustard (Sinapis alba L.) transcribe the same genes in a different developmental context, Mol. Gen. Genrr. (in press) 7 Link, G. (1996) Green life: control of chloroplast gene transcription, BioEssays 18,465471 8 Tanaka, K. et al. (I 997) Characterization of three cDNA species encoding plastid RNA polymerase sigma factors in Arubidopsis rhalianu: evidence for the sigma factor heterogeneity in higher plant plastids, FEBS Len. 4 13. 309-3 I3 9 Isono, K. et al. Leaf-specifically expressed genes for polypeptides destined for chloroplasts with domains of sigma-70 factors of bacterial RNA polymerases in Arabidopsir thulium, Proc. N&l. Acad. Sci. L’. S. A. (in press) 10 Iratni, R. et al. (1997) Organ-specific transcription of the rm operon in spinach plastids, J. Biol. Chern. 272. 13676-13682 11 Baginsky. S., Tiller, K. and Link, G. (1997) Transcription factor phosphorylation by a protein kinase associated with chloroplast RNA polymerase from mustard (Sinapis &ha). Plant Mol. Biol. 34. 181-189
literature
12 Morden, C.W. et al. (1991) Plastid translation and transcription genes in a non-photosynthetic plant: intact. missing and pseudo genes, EMBO .I. IO. 3281-3288 13 Hess, W.R. ef al. (1993) Chloroplast rpsl5 and the rpoB/CI/CZ gene cluster are strongly transcribed in ribosome-deficient plastids: evidence for a functioning non-chloroplastencoded RNA polymerase, EMBO J. 12.563-571 14 Han, CD. er al. (1993) Aberrations in plastid transcripts and deficiency of plastid DNA in striped and albino mutants in maize, Planra 19 I, 552-563 15 Htibschmann. T. and Bomer, T. Characterization of transcription initiation sites in ribosomedeficient barley plastids. Plant Mol. Biol. (in preai) 16 Kapoor, S.. Suzuki, J.Y. and Sugiura. M. ( 1997) Identification and functional significance of a new class of non-consensus-type plastid promoters. Piunr .I. 1 I, 327-337 17 Lerbs-Mache. S. (1993) The I 10.kDa polypeptide of spinach plastid DNA-dependent RNA polymerase: single-subunit enzyme or catalytic core of multimeric enzyme complexes? Proc. Nd. Acad. Sci. U. S. A. 90, 5509-5513 18 DuBell, A.N. and Mullet, J.E. (1995) Differential transcription of pea chloroplast genes during lightinduced leaf development, Plant Phyiol. 109. 105-l 12
focus
Receptor signaling and cell proliferation Williams,
R.W., Wilson, J.M. and Meyerowitz, E.M. (1997) A possible role for kinase-associated protein phosphatase in the Arabidopsis CLAVATAl signaling pathway, Proc. Nat/. Acad. Sci. U. S. A. 94,10467-10472
Biochemical assaysrevealed that the aphenotypesimilarto thatproducedby weak In Arabidopsis, mutationsin CLAVATAl kinase domainof CLVl autophosphorylatesclvl alleles.Thebasisof this wasexamined (CLVl) give riseto plantswith muchlarger but that autophosphoryl- usingafilter-bindingassay:fusionconstructs apicalandfloral meristems, suggesting that on serineresidues, CLVl actsto represscell proliferation.The ation is greatly reducedby the clvl -I mu- of KAPP andCLVl werefound to interact, sequence of CLVl appears to encodeamem- tation.Whenan inactiveform of the kinase andtheextentof thisinteractionwasdependstatusof CLVI. brane-bound receptorkinase,comprisingan wasincubatedwith theCLV 1kinase,thistoo ent on the phosphorylation revealingthatCLVl can Furthermore,the CLVl kinaseis able to N-terminal signal sequence;a seriesof wasphosphotylated, catalysethe phosphorylationof KAPP, and Theexpression of CLVl leucine-richrepeats;a membrane-spanningtransphosphorylate. CLVl. domain;andacytoplasmickinasedomain.In RNA is primarilyconfinedto the subepider- in turn KAPP can dephosphorylate There Thus,thedataindicatethatKAPP negatively an attempt to dissectthe CLVl signaling malcellsof apicalandfloralmeristems. pathway, Meyerowitz’s grouphave exam- is a similar.but moreextensive,patternof regulatesCLVl signaling,but its expression protein patternandability to interactwith otherrecepined the kinaseactivity of CLVl and its expressionof a kinase-associated suggests that it mayalsoplay ability to interact with other regulatory phosphatase(KAPP). Overexpressionof tor-likekinases a role in other transduction pathways. KAPP cDNA in transgenic plants gave rise to components.
6
January 1998, Vol. 3, No. 1
Copyright
0 1998 Elsevier Science Ltd. All rights reserved. 1360 - 1385/98/$19.00