- Email: [email protected]
Recipient heart transplantation: NMR studies
J Mel Cell Cardiol 23 (Supplement
34
CHANGES INFUSION. Cardiology
IV) (1991)
IN SERUM HAGNESIUM AND POTASSIUM B. Brembilla-Perrot, A. Terrier de 54500 VANDOmE FRANCE
la
DURING Chaise
ISOPROTERENOL
Some ventricular arrhythmias have recently been related to decrease in magnesium (Mg) level. Because adrenaline causes decrease in serum (S) potassium (K) and S Mg, the purpose of the study was to look for isoproterenol (Is) for a changes in S K under and S Mg and correlation with induction of tachycardias. Programmed atria1 and ventricular stimulation was performed in 68 pts before to and under infusion of 20-30 Mg Is. 15 pts had inducible tachycardias under Is. S Mg and S K were measured at the end of each stimulation in the control state and under Is. A decrease in S Mg and S K was always noted but only changes in SK were significant : S Mg : 20.47 _+ 2.06 + 19.92 f 1.99 mg/l (NS), S K : 3.8 f 0.4 mEq --t 3.51 + 0.45 (p
35
RECIPIENT HEART TRANSPLANTATION : NMR STUDIES. F. Brunotte, G. Pinelli. J.M. Bscanye. B. Peiffert, J.P. Carteaux, P.M. Mertes, J.P. Villemot. Chigie Cardiaque et Trans&ntations Cardio.Thoraciques. CHU Brabois. 54511 Vandoeuvreles-Nancy - Laboratoire de Biophysique, roe Lionnois, %ooO Nancy. Magnetic resonance spectroscopy (MRS) has proven to be useful for studying the nwabolism of isolated perfused hearts. We have assessed here by MRS donor and recipient hearts in a program of heart lxansplantation. Thirteen hearts have beem studied, in this preliminaryreport 11 recipknu hearts, and 2 donors hearts (one was reimplanted). After cardioplegic arrest, the hearts are placed in sterile plastic bags with a saline solution at 4r. MRS is performed less than 30 min after excision.andcontinuedthereafteafor recipienthearts.Spectraareoblainedat2.4 tesla,inaBruker Biospec horizontalbore magnet Hearts are not removed from the gabs for meawement, and a surface coil 5 cm in dieter is used. Results are expressed as ratio relatively to the beta-Al? (ATP) peak : phosphocreatine (PCr)/ATP. inorganic phosphates (Pi)/ATP, (F'i+ PCr)/ATP. The pH is calcubited from the chemical shift between PCr and Pi. In the hvo donors’hcarts, PCr/ATP has ken found to be in the same range as in open chest pig in viva. After 9 hours excision, PCr was still detectable in one non reimpkmted donor heart. PCr decreases in excised donor human heart much slowly than in hearts from small animals. In failing heart, (PCr+(Pi))/ATP is significantly lower in infarcted hear& but can be nearly normal in dilated cardiomyopathy. The reduced ratio PCr/ATP observed in ischemic heart disease8 could reflect metabolic adapt&on. The= preliminary results provide informations on donor heart and failing heart. These data would conttibute to a be.ttex understanding of in viva spectra. MRS examination of donor heart without delaying the intezvention could be a useful adjunct to evaluate the predictive viability of the graft.
36 INFLUENCE
OF 8-(N,N-DIETHYLAMINO)OCTYL-3,4,5-TRIMETROXYBENZOATE (TMB8) ON CELL CYCLE PROGRESSION AND PROLIFERATION OF CULTURBD ARTERIAL SMOOTH MUSCLE CELLS. PI. Campan, A-P. Gadeau, D. Millet, N. Guerineau, P. Mollard, G. Razaka, and C. Desgranges. Inserm, Unite 8 de Cardiologie, 33600 PESSAC
8-(N,N-diethylamino)octyl-3,4,5triPethoxybenzoate (TMB-8) a putative inhibitor of intracellular calcium mobilization, causes a dosedependent inhibition of serum-induced proliferation of arterial smooth muscle cells in culture. Neither early rise in cytosolic calcium concentration nor induction of early induced cell cycle ornithine decarboxylase) are inhibited after dependent genes (c-fos, serum stimulation in Dresence of 100 uM m-8. In contrast, expression of thymid& kinase, a ge& normally induced in late-G1 phase, is entirely inhibited by TMB-8. Taken together with flow cytometry studies; these resulis indicate that M-8 blocks cell cycle progression in mid- or late-G1 phase by a mechanism not directly related to early responses to serum stimulation since TMB-8 is also effective when introduced several hours after serum stimulation. s.12
34
CHANGES INFUSION. Cardiology
IV) (1991)
IN SERUM HAGNESIUM AND POTASSIUM B. Brembilla-Perrot, A. Terrier de 54500 VANDOmE FRANCE
la
DURING Chaise
ISOPROTERENOL
Some ventricular arrhythmias have recently been related to decrease in magnesium (Mg) level. Because adrenaline causes decrease in serum (S) potassium (K) and S Mg, the purpose of the study was to look for isoproterenol (Is) for a changes in S K under and S Mg and correlation with induction of tachycardias. Programmed atria1 and ventricular stimulation was performed in 68 pts before to and under infusion of 20-30 Mg Is. 15 pts had inducible tachycardias under Is. S Mg and S K were measured at the end of each stimulation in the control state and under Is. A decrease in S Mg and S K was always noted but only changes in SK were significant : S Mg : 20.47 _+ 2.06 + 19.92 f 1.99 mg/l (NS), S K : 3.8 f 0.4 mEq --t 3.51 + 0.45 (p
35
RECIPIENT HEART TRANSPLANTATION : NMR STUDIES. F. Brunotte, G. Pinelli. J.M. Bscanye. B. Peiffert, J.P. Carteaux, P.M. Mertes, J.P. Villemot. Chigie Cardiaque et Trans&ntations Cardio.Thoraciques. CHU Brabois. 54511 Vandoeuvreles-Nancy - Laboratoire de Biophysique, roe Lionnois, %ooO Nancy. Magnetic resonance spectroscopy (MRS) has proven to be useful for studying the nwabolism of isolated perfused hearts. We have assessed here by MRS donor and recipient hearts in a program of heart lxansplantation. Thirteen hearts have beem studied, in this preliminaryreport 11 recipknu hearts, and 2 donors hearts (one was reimplanted). After cardioplegic arrest, the hearts are placed in sterile plastic bags with a saline solution at 4r. MRS is performed less than 30 min after excision.andcontinuedthereafteafor recipienthearts.Spectraareoblainedat2.4 tesla,inaBruker Biospec horizontalbore magnet Hearts are not removed from the gabs for meawement, and a surface coil 5 cm in dieter is used. Results are expressed as ratio relatively to the beta-Al? (ATP) peak : phosphocreatine (PCr)/ATP. inorganic phosphates (Pi)/ATP, (F'i+ PCr)/ATP. The pH is calcubited from the chemical shift between PCr and Pi. In the hvo donors’hcarts, PCr/ATP has ken found to be in the same range as in open chest pig in viva. After 9 hours excision, PCr was still detectable in one non reimpkmted donor heart. PCr decreases in excised donor human heart much slowly than in hearts from small animals. In failing heart, (PCr+(Pi))/ATP is significantly lower in infarcted hear& but can be nearly normal in dilated cardiomyopathy. The reduced ratio PCr/ATP observed in ischemic heart disease8 could reflect metabolic adapt&on. The= preliminary results provide informations on donor heart and failing heart. These data would conttibute to a be.ttex understanding of in viva spectra. MRS examination of donor heart without delaying the intezvention could be a useful adjunct to evaluate the predictive viability of the graft.
36 INFLUENCE
OF 8-(N,N-DIETHYLAMINO)OCTYL-3,4,5-TRIMETROXYBENZOATE (TMB8) ON CELL CYCLE PROGRESSION AND PROLIFERATION OF CULTURBD ARTERIAL SMOOTH MUSCLE CELLS. PI. Campan, A-P. Gadeau, D. Millet, N. Guerineau, P. Mollard, G. Razaka, and C. Desgranges. Inserm, Unite 8 de Cardiologie, 33600 PESSAC
8-(N,N-diethylamino)octyl-3,4,5triPethoxybenzoate (TMB-8) a putative inhibitor of intracellular calcium mobilization, causes a dosedependent inhibition of serum-induced proliferation of arterial smooth muscle cells in culture. Neither early rise in cytosolic calcium concentration nor induction of early induced cell cycle ornithine decarboxylase) are inhibited after dependent genes (c-fos, serum stimulation in Dresence of 100 uM m-8. In contrast, expression of thymid& kinase, a ge& normally induced in late-G1 phase, is entirely inhibited by TMB-8. Taken together with flow cytometry studies; these resulis indicate that M-8 blocks cell cycle progression in mid- or late-G1 phase by a mechanism not directly related to early responses to serum stimulation since TMB-8 is also effective when introduced several hours after serum stimulation. s.12