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REDUCED ADIPONECTIN LEVEL IS ASSOCIATED WITH SEVERITY OF CORONARY ARTERY DISEASE
Publication Only small dense LDL and high levels of small dense LDL-cholesterol are very promising risk marker for CHD. 117
REDUCED ADIPONECTIN LEVEL IS ASSOCIATED WITH SEVERITY OF CORONARY ARTERY DISEASE
K. Zengin 1 , M.S. Ulgen 1 , S. Buyukbas 2 . 1 Selcuk University, Meram Faculty of Medicine, Department of Cardiology; 2 Selcuk University, Meram Faculty of Medicine, Department of Biochemistry Background and aims: Adiponektin is a hormone secreted by adipocytes. In humans, low plasma adiponectin levels have been associated with obesity, diabetes, and coronary artery disease. High plasma adiponectin concentrations reportedly have a significantly lower risk of myocardial infarction, independent of traditional cardiovascular risk factors such as hyperlipidemia, diabetes, and hypertension. In this study, the relationship between the plasma adiponectin, leptin levels and the severity of coronary artery disease, as assessed using the Gensini score (GS), an index for the severity of coronary artery stenosis, was investigated in patients with ST-segment elevation myocardial infarction (STEMI) Material and methods: The subjects of the study were 110 patients (20 women;mean age:66±15years and 90 men;mean age:60±11years) with STEMI who underwent coronary angiography. Serum concentrations of adiponectin and leptin were measured by using a enzyme-linked immunosorbent assay. The location and extent of coronary artery occlusions were assessed using Gensini score. SPSS statistical computing program version 12.0 was used for data analysis. P values <0.05 were considered statistically significant. Results: The serum adiponectin levels were lower in patients than the control (2.36±1.97 ng/dl, 4.62±1.44 ng/dl p<0,001). The plasma adiponectin levels were inversely correlated with leptin levels (r=-236,P = 0.005). After adjustment for conventional risk factors for cardiovascular diseases, there was a significant correlation between the serum adiponectin level and the GS (r=-236, p=0.034) but leptin levels showed no association with the severity of coronary atherosclerosis Conclusions: The measurement of plasma adiponectin levels may be useful for predicting the severity of coronary artery stenosis.
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Background and aim: Major differences on IMT among diabetic population have been reported, especially between IGT,T2DM versus normal. The determination of the association of IMT with plasma glucose levels in diabetics and the influence of atheromatic factors on IMT, those existing in our material have been evaluated. Methods: B- mode u/s of common carotid artery (CCA) was performed with a siemens u/s, using a 7,5 MHZ transducer, on 157 individuals with mean age of 63,8±10,17 years. The IMT was assessed in plaque–free portions of the distal 10 mm of CCA. The material was classified in three groups: a) non IGT subjects with mild increase of fasting plasma glucose values: 106,02±1,152 mg/dl, n=52. b) IGT patients: 111,94±1,50mg/dl, n=58 c) T2DM: 130,48± 2,57mg/dl, n=47. Data was analysed with student t test, one way anova and partial correlation and linear multivariate regression. Results: The mean values of IMT was 0.59± 0,03mm for non IGT, 0.58± 0,02 mm for IGT and 0.58± 0,02 mm for T2DM. Values of IMT was increased, but non-significant differences among three groups was found. In the non- IGT group correlation was found between IMT and age (p=0,025), triglycerides (p=0,038), atheromatic index, (p=0,022), hypertension (p=0,075), homocystein (p=0,029) and HDL (p=0,01). Conclusion: Individuals in high risk for T2DM, with borderline but not diagnostic levels of FPG and several other factors for atherosclerosis, have already increased values of IMT, as macrovascular disease starts before the intolerance of glucose.
K. Zengin, M.S. Ulgen. Selcuk University, Meram Faculty of Medicine, Department of Cardiology Background and aims: Osteopontin is an adhesion molecule which was first identified in bone tissue. It has been implicated as a major mediator of the profibrotic changes induced by angiotensin II during heart failure. Osteopontin has been identified in cultured neonatal and adult rat cardiac fibroblasts and is found in macrophages in necrotic areas in rat hearts. The aim of this study was to investigate the association between plasma levels and gensini score (GS) in patients with ST-segment elevation myocardial infarction (STEMI) Material and methods: We measured baseline osteopontin levels in stored serum samples of 110 patients (20 women; mean age: 66±15 years and 90 men; mean age: 60±11 years) with acut STEMI and in 30 controls. Exclusion criteria were: heart, lung, bone, collagen, malignant diseases, impaired liver or kidney function. The plasma osteopontin concentration was measured using a commercially available enzyme immunoassay kit. The location and extent of coronary artery occlusions were assessed using GS. The Pearson’s correlation analysis was performed to estimate correlations between variables Results: Osteopontin levels were significantly higher in the patients than control (acut STEMI: 250.47±62.96 ng/dl, control: 48.53±41.98 ng/dl). The plasma osteopontin concentrations correlated positively with age (r=0.177, p=0.003), smoking (r=0.350, p=0.0001) fibrinogen levels (r=0.22, p<0.002). There was a significant correlation between osteopontin levels and GS (r=0.192, p<0,042) Conclusions: The present study shows that plasma levels of osteopontin are significantly elevated in patients with acut STEM. Moreover our data suggest that elevated osteopontin levels are associated with the extent of coronary atherosclerosis. Future studies in larger patient cohorts will have to show the potential clinical relevance of this novel biomarker.
EFFECTS OF EFFLUX TRANSPORTER BCRP C421A POLYMORPHISM ON PITAVASTATIN PHARMACOKINETICS AND INTERACTION WITH GRAPEFRUIT JUICE
B. Tomlinson 1 , V. Mak 1 , M. Hu 1 , O. Yin 2 , M. Chow 2 . 1 Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR; 2 School of Pharmacy, The Chinese University of Hong Kong, Hong Kong SAR Background and aims: Pitavastatin is scarcely metabolised, but its distribution is influenced by influx and efflux transporters including the breast cancer resistance protein (BCRP, gene ABCG2). We examined the effect of a common functional polymorphism in ABCG2 (C421A) on the pharmacokinetics of pitavastatin and its lactone and the interaction with grapefruit juice. Methods: Twelve healthy male volunteers were genotyped for the ABCG2 C421A polymorphism and took oral pitavastatin 2 mg. Plasma concentrations of pitavastatin acid and the lactone metabolite were measured over 48 hours. The study was repeated with administration of 200 ml double-strength grapefruit juice with the pitavastatin. Results: Pharmacokinetic parameters of Cmax, AUC0-48 and AUC0-inf for the acid and the lactone were analyzed according to genotypes. The subjects included 5 homozygous for 421CC, 5 heterozygous for 421CA and 2 were homozygous for 421AA. Taking the grapefruit juice had no significant effect on the pharmacokinetic parameters of pitavastatin acid and lactone. Both the AUC0-48 and AUC0-inf tended to be higher for pitavastatin acid and lactone in homozygous 421CC subjects than in the other two groups, but this was not statistically significant, with or without grapefruit juice. Conclusion: Pharmacokinetic properties of pitavastatin acid and lactone were not affected by the presence of grapefruit juice. The ABCG2 C421A polymorphism tended to have an effect on the pharmacokinetic parameters but this was not significant. Larger studies, including examination of interactions with other transporters, are needed to clarify these data.
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
PUBLICATION ONLY
THE ASSOCIATION BETWEEN THE CONCENTRATION OF OSTEOPONTIN AND THE EXTENT OF CORONARY ATHEROSCLEROSIS
MEASUREMENT OF CAROTID INTIMAL-MEDIAL THICKNESS (IMT) IN DIABETICS WITH SEVERAL OTHER RISK FACTORS FOR ATHEROSCLEROSIS
P. Voudouri 1 , S. Bakatselos 2 , V. Tsapas 2 , A. Kalyvinos 2 , A. Sakalerou 2 , G. Papadopoulos 2 , A. Tavridou 3 , T. Daskalou 4 , H. Chatzieleftheriou 5 . 1 Department of Radiology, General Hospital of Drama, Drama, Greece; 2 Department of Pathology, General Hospital of Drama, Drama, Greece; 3 Medical School, Democritous University of Thrace, Alexandroupolis, Greece; 4 4Laboratory of Biochemistry, General Hospital of Didimotiho, Didimotiho, Greece; 5 3Department of Cardiology,General Hospital of Drama, Drama, Greece
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REDUCED ADIPONECTIN LEVEL IS ASSOCIATED WITH SEVERITY OF CORONARY ARTERY DISEASE
K. Zengin 1 , M.S. Ulgen 1 , S. Buyukbas 2 . 1 Selcuk University, Meram Faculty of Medicine, Department of Cardiology; 2 Selcuk University, Meram Faculty of Medicine, Department of Biochemistry Background and aims: Adiponektin is a hormone secreted by adipocytes. In humans, low plasma adiponectin levels have been associated with obesity, diabetes, and coronary artery disease. High plasma adiponectin concentrations reportedly have a significantly lower risk of myocardial infarction, independent of traditional cardiovascular risk factors such as hyperlipidemia, diabetes, and hypertension. In this study, the relationship between the plasma adiponectin, leptin levels and the severity of coronary artery disease, as assessed using the Gensini score (GS), an index for the severity of coronary artery stenosis, was investigated in patients with ST-segment elevation myocardial infarction (STEMI) Material and methods: The subjects of the study were 110 patients (20 women;mean age:66±15years and 90 men;mean age:60±11years) with STEMI who underwent coronary angiography. Serum concentrations of adiponectin and leptin were measured by using a enzyme-linked immunosorbent assay. The location and extent of coronary artery occlusions were assessed using Gensini score. SPSS statistical computing program version 12.0 was used for data analysis. P values <0.05 were considered statistically significant. Results: The serum adiponectin levels were lower in patients than the control (2.36±1.97 ng/dl, 4.62±1.44 ng/dl p<0,001). The plasma adiponectin levels were inversely correlated with leptin levels (r=-236,P = 0.005). After adjustment for conventional risk factors for cardiovascular diseases, there was a significant correlation between the serum adiponectin level and the GS (r=-236, p=0.034) but leptin levels showed no association with the severity of coronary atherosclerosis Conclusions: The measurement of plasma adiponectin levels may be useful for predicting the severity of coronary artery stenosis.
119
Background and aim: Major differences on IMT among diabetic population have been reported, especially between IGT,T2DM versus normal. The determination of the association of IMT with plasma glucose levels in diabetics and the influence of atheromatic factors on IMT, those existing in our material have been evaluated. Methods: B- mode u/s of common carotid artery (CCA) was performed with a siemens u/s, using a 7,5 MHZ transducer, on 157 individuals with mean age of 63,8±10,17 years. The IMT was assessed in plaque–free portions of the distal 10 mm of CCA. The material was classified in three groups: a) non IGT subjects with mild increase of fasting plasma glucose values: 106,02±1,152 mg/dl, n=52. b) IGT patients: 111,94±1,50mg/dl, n=58 c) T2DM: 130,48± 2,57mg/dl, n=47. Data was analysed with student t test, one way anova and partial correlation and linear multivariate regression. Results: The mean values of IMT was 0.59± 0,03mm for non IGT, 0.58± 0,02 mm for IGT and 0.58± 0,02 mm for T2DM. Values of IMT was increased, but non-significant differences among three groups was found. In the non- IGT group correlation was found between IMT and age (p=0,025), triglycerides (p=0,038), atheromatic index, (p=0,022), hypertension (p=0,075), homocystein (p=0,029) and HDL (p=0,01). Conclusion: Individuals in high risk for T2DM, with borderline but not diagnostic levels of FPG and several other factors for atherosclerosis, have already increased values of IMT, as macrovascular disease starts before the intolerance of glucose.
K. Zengin, M.S. Ulgen. Selcuk University, Meram Faculty of Medicine, Department of Cardiology Background and aims: Osteopontin is an adhesion molecule which was first identified in bone tissue. It has been implicated as a major mediator of the profibrotic changes induced by angiotensin II during heart failure. Osteopontin has been identified in cultured neonatal and adult rat cardiac fibroblasts and is found in macrophages in necrotic areas in rat hearts. The aim of this study was to investigate the association between plasma levels and gensini score (GS) in patients with ST-segment elevation myocardial infarction (STEMI) Material and methods: We measured baseline osteopontin levels in stored serum samples of 110 patients (20 women; mean age: 66±15 years and 90 men; mean age: 60±11 years) with acut STEMI and in 30 controls. Exclusion criteria were: heart, lung, bone, collagen, malignant diseases, impaired liver or kidney function. The plasma osteopontin concentration was measured using a commercially available enzyme immunoassay kit. The location and extent of coronary artery occlusions were assessed using GS. The Pearson’s correlation analysis was performed to estimate correlations between variables Results: Osteopontin levels were significantly higher in the patients than control (acut STEMI: 250.47±62.96 ng/dl, control: 48.53±41.98 ng/dl). The plasma osteopontin concentrations correlated positively with age (r=0.177, p=0.003), smoking (r=0.350, p=0.0001) fibrinogen levels (r=0.22, p<0.002). There was a significant correlation between osteopontin levels and GS (r=0.192, p<0,042) Conclusions: The present study shows that plasma levels of osteopontin are significantly elevated in patients with acut STEM. Moreover our data suggest that elevated osteopontin levels are associated with the extent of coronary atherosclerosis. Future studies in larger patient cohorts will have to show the potential clinical relevance of this novel biomarker.
EFFECTS OF EFFLUX TRANSPORTER BCRP C421A POLYMORPHISM ON PITAVASTATIN PHARMACOKINETICS AND INTERACTION WITH GRAPEFRUIT JUICE
B. Tomlinson 1 , V. Mak 1 , M. Hu 1 , O. Yin 2 , M. Chow 2 . 1 Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong SAR; 2 School of Pharmacy, The Chinese University of Hong Kong, Hong Kong SAR Background and aims: Pitavastatin is scarcely metabolised, but its distribution is influenced by influx and efflux transporters including the breast cancer resistance protein (BCRP, gene ABCG2). We examined the effect of a common functional polymorphism in ABCG2 (C421A) on the pharmacokinetics of pitavastatin and its lactone and the interaction with grapefruit juice. Methods: Twelve healthy male volunteers were genotyped for the ABCG2 C421A polymorphism and took oral pitavastatin 2 mg. Plasma concentrations of pitavastatin acid and the lactone metabolite were measured over 48 hours. The study was repeated with administration of 200 ml double-strength grapefruit juice with the pitavastatin. Results: Pharmacokinetic parameters of Cmax, AUC0-48 and AUC0-inf for the acid and the lactone were analyzed according to genotypes. The subjects included 5 homozygous for 421CC, 5 heterozygous for 421CA and 2 were homozygous for 421AA. Taking the grapefruit juice had no significant effect on the pharmacokinetic parameters of pitavastatin acid and lactone. Both the AUC0-48 and AUC0-inf tended to be higher for pitavastatin acid and lactone in homozygous 421CC subjects than in the other two groups, but this was not statistically significant, with or without grapefruit juice. Conclusion: Pharmacokinetic properties of pitavastatin acid and lactone were not affected by the presence of grapefruit juice. The ABCG2 C421A polymorphism tended to have an effect on the pharmacokinetic parameters but this was not significant. Larger studies, including examination of interactions with other transporters, are needed to clarify these data.
77th Congress of the European Atherosclerosis Society, April 26–29, 2008, Istanbul, Turkey
PUBLICATION ONLY
THE ASSOCIATION BETWEEN THE CONCENTRATION OF OSTEOPONTIN AND THE EXTENT OF CORONARY ATHEROSCLEROSIS
MEASUREMENT OF CAROTID INTIMAL-MEDIAL THICKNESS (IMT) IN DIABETICS WITH SEVERAL OTHER RISK FACTORS FOR ATHEROSCLEROSIS
P. Voudouri 1 , S. Bakatselos 2 , V. Tsapas 2 , A. Kalyvinos 2 , A. Sakalerou 2 , G. Papadopoulos 2 , A. Tavridou 3 , T. Daskalou 4 , H. Chatzieleftheriou 5 . 1 Department of Radiology, General Hospital of Drama, Drama, Greece; 2 Department of Pathology, General Hospital of Drama, Drama, Greece; 3 Medical School, Democritous University of Thrace, Alexandroupolis, Greece; 4 4Laboratory of Biochemistry, General Hospital of Didimotiho, Didimotiho, Greece; 5 3Department of Cardiology,General Hospital of Drama, Drama, Greece
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