- Email: [email protected]
Regression in basal cell carcinoma
66
Poster Presentations
315 FSTABLISHYENT AND CHARACTERIZATION OF A HUMAN CELL LINE RAVING ROTH SURFACE MARKERS OF YONOCYTE-YACROPHACE AND NATURAL KILLFR CI3.L l,INFACE FROM A PATIENT WITH ATOPIC DERYATITIS. Yoshtakl Hamnmoto. Ko,, Naval, Hlroko Furumoto. Yasahtko Yuto and ChIdor m Department of DrrmntoloRV. Yamaguchl lln~vrrs~ty School of Yrdtcine. Ubc. Japan lnrgc amounts of homogcnous crlls arc not always In grnrral, nvn!labl? for ,n vltrostudlrs of Inflammatory skin disorder. such as atoplr drrmatItls. To ohtaln unrful model sy.st~ms. IC trlrd to rstabllsh a cell l!nr from prrlphcral blood of a patlent wtth ntopic drrmntltls having an increased srrwn lewl of total I@ About 2 months aftrr cultlvatlon of mononuclrar cells. thry hrcamr This IIOC. termed YAA. shored a to proiiferatlvr I” s”spr”s1o”. positive rcwtuon for o-naphthyl butyrate estcraw rahlch was row plrt~ly lnhtbltrd by sodium fluorldr. YAA crlls had surfare markrrs reacted w,th CDd. CDIlh and CD33 hut neither rlth CD2. CD3 nor CDR. Interrst~ngly. YAA rrlls had CD56 molecule. one of the markYoreovrr. YAA cells prorrs sprclflr for natural klllcr cells. dwcd consldrrahle amounts of tumor necrosis factor-o rhrn trratcd Thesr ftndings suggest that YAA rells havr with phorbol PS~PT. sow proprrt~cs spcclflr for fh? monocyte-macrophage I~wngr. Thtis nels Itnr might br vnluahlc for studyinfi not only the pathogrnrsrs of atopar dcrmatltls and thr rcpulatlon ot monocyf?-marrophapc dtffcrrnt,atlon hut also the or,$,n of natural klllrr rrlls.
316
319 RLIGRI~SSION IN UASAL C[il.l CARCINOAIA. bLi( hcllc I. Ilunt, Carv hl. Hallida\~ and Rosa SIC. Bdrnetson. Dcpxtment of Dermatology. University of Sydney. Sydney. N.S.W.. AustrAI;I Spontaneous regression of some wtawous turnours is well recognised, and is thought to result from an immutwlogiral response tr) the tumour. Regression ha previously been noted in basal cell carcinomas liX:(:l hut nu prior in\cstigatirm\ defining the role of the immune recpunse in the rcgrc\sion 01 this malignanry have been pcrformcd. We have cuamined 45
primary (&CC) (20 nodular. 2.5 superfic iall .tnd identified the cellular phenotypes end acti\Jtion states of the cell\ infiltrating primar)’ rcgrcssing and non-regreGng KC 1~). immunocytochcmistr).. We h&e found rt signifIcantI), int rused numbers of CD3+ and CD-++ ‘T cells infiltrating regressing compared to non-rcgrcssing turnours, and the wprcssion 01 interleukin-2 reteptor (an early avrivation mirhcr lor T
317
320
ISOLATION AND FROM INFANTILE
PRELIMINARY STUDY ON IMMUNOTHERAPY FOR ADULT T-CELL LEUKEMIA - INDUCTION OF HUMAN T LYMPHOTROPIC VIRUS TYPE ISPECIFIC CYTOTOXIC T LYMPHOCYTES Yoshlhlko Katahwa’. M~tsuru Setoyama’. Tamow Kanzalo’, and Shunro Sonoda’, Department of ‘Dermatology,
CHARACTERIZATION OF ENWTHELIAL CELLS HEMANGIOENDOTHELIOMA. Chun_Pin.Makl.2, Depanmenu of Dermatology, RNCCR. aodz, ‘National Cheng Kung University Hospital.Tainan, Taiwan, ROC and Qanford University School ofMedicine, Stanford, California, USA. The factors that induce either the growth or regressionof hemangioma are not understood. To obtain information on the mechanismsthat control the growth of cndothclial cells in hemangioma. we have developed methods to isolate endothelial cells, maintaintheminculture. and have compared the characteristics ofthese cells with normal demml microvascularendothelial cells. Four cases of infantile hemangioendothelioma (IHE) was used as culture material in this study. Endothelial cells were isolated from split thicknesssections cut with a Castroviejo kcratotome and then releasedfrom the sections by enzyme digestion. Cells isolated from IHE were comprised of two distinct types of morphology. one in cpithelioid and the other in spindle shape. Each of these types of cells showed a different expression of factor VIII and factor XIIIa. Hence, cells isolated from IHE were strickingly diGrent from normal dcrmal microvaxular cndothelial cells in the respect of morphology and expression of biological markers. These unique properties of endothelial cells may provide new insights into the treatment ofhemangioma.
isolated
‘Virology. Faculty of Med~cme. Kagoshlma Unwers~ty. Kagoshlma, Japan Prognosis of adult T-cell leukemra (ATL) IS very poor. and non of therapreshave been successful 10 ,nduce complae re,,,,ss,on. Thus, more spec,f,c and stronger therapies are demanded to treat ATL. Immunotherapy. such as vaccmat!on or lnfuslon of cytotox,c T lymphocyles (CTLa). would be the one. We Lned ,n vitro lnductmn of CTLs from peripheral Mocd lymphocytes (PBLS) t healthy m&v!dual (HI). 4 human T lymphotropx &us type I (HTLC-I) &e&d asymptomattc camen (ACs), and 3 AIL oatvws. PBLs were stimulated with a HLA matched HTLV-I-mfectedT.cell line m kPMI-1640 medmm supplementedwth LOWFCS, rIL-I. rlL-2. rIL-4. rIL-6 and rlL7. HTLV-l-m&ted Tcell bne was macwated wth heat veaunem (56C. 3Omm) before sumulauon. These combmaoons were round to te the best to obtam CTL after various tnals. Four weeks laler. responder cells were harvested, and screened for CylotOXlC acttwty to “anow HTLV-I-mfected T-cell hnes and B-cells expressmg HTLV-I protems. As results 5 HLA-A24 restncted CDS CTLs and 5 HLA-DRl5 restncled CD4’ CTLs were estabhshed from I/t HI. 414 ACs and 113 ATL patlent Each CTL recognrzed one OTtwo HTLV-I specllx epitopes Bes~d-es, one HLA unrestncted CD4’ CTL obtamed from ATL pabent d,d no, rea,Rn,ze KTLV-I gene pdUCtS. but ,t blled ATL cells. These results suggested that the HTLV-I speclfx ~Ls Induced by the method &err&d above mtgh: be useful for specitic unmunolherapy for ATL &went%
Poster Presentations
315 FSTABLISHYENT AND CHARACTERIZATION OF A HUMAN CELL LINE RAVING ROTH SURFACE MARKERS OF YONOCYTE-YACROPHACE AND NATURAL KILLFR CI3.L l,INFACE FROM A PATIENT WITH ATOPIC DERYATITIS. Yoshtakl Hamnmoto. Ko,, Naval, Hlroko Furumoto. Yasahtko Yuto and ChIdor m Department of DrrmntoloRV. Yamaguchl lln~vrrs~ty School of Yrdtcine. Ubc. Japan lnrgc amounts of homogcnous crlls arc not always In grnrral, nvn!labl? for ,n vltrostudlrs of Inflammatory skin disorder. such as atoplr drrmatItls. To ohtaln unrful model sy.st~ms. IC trlrd to rstabllsh a cell l!nr from prrlphcral blood of a patlent wtth ntopic drrmntltls having an increased srrwn lewl of total I@ About 2 months aftrr cultlvatlon of mononuclrar cells. thry hrcamr This IIOC. termed YAA. shored a to proiiferatlvr I” s”spr”s1o”. positive rcwtuon for o-naphthyl butyrate estcraw rahlch was row plrt~ly lnhtbltrd by sodium fluorldr. YAA crlls had surfare markrrs reacted w,th CDd. CDIlh and CD33 hut neither rlth CD2. CD3 nor CDR. Interrst~ngly. YAA rrlls had CD56 molecule. one of the markYoreovrr. YAA cells prorrs sprclflr for natural klllcr cells. dwcd consldrrahle amounts of tumor necrosis factor-o rhrn trratcd Thesr ftndings suggest that YAA rells havr with phorbol PS~PT. sow proprrt~cs spcclflr for fh? monocyte-macrophage I~wngr. Thtis nels Itnr might br vnluahlc for studyinfi not only the pathogrnrsrs of atopar dcrmatltls and thr rcpulatlon ot monocyf?-marrophapc dtffcrrnt,atlon hut also the or,$,n of natural klllrr rrlls.
316
319 RLIGRI~SSION IN UASAL C[il.l CARCINOAIA. bLi( hcllc I. Ilunt, Carv hl. Hallida\~ and Rosa SIC. Bdrnetson. Dcpxtment of Dermatology. University of Sydney. Sydney. N.S.W.. AustrAI;I Spontaneous regression of some wtawous turnours is well recognised, and is thought to result from an immutwlogiral response tr) the tumour. Regression ha previously been noted in basal cell carcinomas liX:(:l hut nu prior in\cstigatirm\ defining the role of the immune recpunse in the rcgrc\sion 01 this malignanry have been pcrformcd. We have cuamined 45
primary (&CC) (20 nodular. 2.5 superfic iall .tnd identified the cellular phenotypes end acti\Jtion states of the cell\ infiltrating primar)’ rcgrcssing and non-regreGng KC 1~). immunocytochcmistr).. We h&e found rt signifIcantI), int rused numbers of CD3+ and CD-++ ‘T cells infiltrating regressing compared to non-rcgrcssing turnours, and the wprcssion 01 interleukin-2 reteptor (an early avrivation mirhcr lor T
317
320
ISOLATION AND FROM INFANTILE
PRELIMINARY STUDY ON IMMUNOTHERAPY FOR ADULT T-CELL LEUKEMIA - INDUCTION OF HUMAN T LYMPHOTROPIC VIRUS TYPE ISPECIFIC CYTOTOXIC T LYMPHOCYTES Yoshlhlko Katahwa’. M~tsuru Setoyama’. Tamow Kanzalo’, and Shunro Sonoda’, Department of ‘Dermatology,
CHARACTERIZATION OF ENWTHELIAL CELLS HEMANGIOENDOTHELIOMA. Chun_Pin.Makl.2, Depanmenu of Dermatology, RNCCR. aodz, ‘National Cheng Kung University Hospital.Tainan, Taiwan, ROC and Qanford University School ofMedicine, Stanford, California, USA. The factors that induce either the growth or regressionof hemangioma are not understood. To obtain information on the mechanismsthat control the growth of cndothclial cells in hemangioma. we have developed methods to isolate endothelial cells, maintaintheminculture. and have compared the characteristics ofthese cells with normal demml microvascularendothelial cells. Four cases of infantile hemangioendothelioma (IHE) was used as culture material in this study. Endothelial cells were isolated from split thicknesssections cut with a Castroviejo kcratotome and then releasedfrom the sections by enzyme digestion. Cells isolated from IHE were comprised of two distinct types of morphology. one in cpithelioid and the other in spindle shape. Each of these types of cells showed a different expression of factor VIII and factor XIIIa. Hence, cells isolated from IHE were strickingly diGrent from normal dcrmal microvaxular cndothelial cells in the respect of morphology and expression of biological markers. These unique properties of endothelial cells may provide new insights into the treatment ofhemangioma.
isolated
‘Virology. Faculty of Med~cme. Kagoshlma Unwers~ty. Kagoshlma, Japan Prognosis of adult T-cell leukemra (ATL) IS very poor. and non of therapreshave been successful 10 ,nduce complae re,,,,ss,on. Thus, more spec,f,c and stronger therapies are demanded to treat ATL. Immunotherapy. such as vaccmat!on or lnfuslon of cytotox,c T lymphocyles (CTLa). would be the one. We Lned ,n vitro lnductmn of CTLs from peripheral Mocd lymphocytes (PBLS) t healthy m&v!dual (HI). 4 human T lymphotropx &us type I (HTLC-I) &e&d asymptomattc camen (ACs), and 3 AIL oatvws. PBLs were stimulated with a HLA matched HTLV-I-mfectedT.cell line m kPMI-1640 medmm supplementedwth LOWFCS, rIL-I. rlL-2. rIL-4. rIL-6 and rlL7. HTLV-l-m&ted Tcell bne was macwated wth heat veaunem (56C. 3Omm) before sumulauon. These combmaoons were round to te the best to obtam CTL after various tnals. Four weeks laler. responder cells were harvested, and screened for CylotOXlC acttwty to “anow HTLV-I-mfected T-cell hnes and B-cells expressmg HTLV-I protems. As results 5 HLA-A24 restncted CDS CTLs and 5 HLA-DRl5 restncled CD4’ CTLs were estabhshed from I/t HI. 414 ACs and 113 ATL patlent Each CTL recognrzed one OTtwo HTLV-I specllx epitopes Bes~d-es, one HLA unrestncted CD4’ CTL obtamed from ATL pabent d,d no, rea,Rn,ze KTLV-I gene pdUCtS. but ,t blled ATL cells. These results suggested that the HTLV-I speclfx ~Ls Induced by the method &err&d above mtgh: be useful for specitic unmunolherapy for ATL &went%