- Email: [email protected]
Relationship between LDL subfractions and postprandial lipemia in healthy subjects
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Abstracts / Atherosclerosis 263 (2017) e111ee282
group), DMEM supplemented 100mg/L glucose (Glucose group) or 100mg/ L fructose (Fructose group) for 1hour and protein and RNA was collected. mRNA expression was measured by RT-qPCR and protein expression was measured by western blotting. Results: Compared with control group, MTTP mRNA expression in glucose andreased fructose group was increased. MOGAT2 mRNA expression was increased only in fructose group. In protein expression, MTP and MGAT2 were increased in glucose and fructose group, and chREBP, SREBP-1 were upregulated only in fructose group. Conclusions: In caco-2 cells as enterocyte model, short-term fructose exposure increased lipogenic enzyme gene and protein expression such as MTP and MGAT2 by activating transcriptionfactors as chREBP and SREBP-1. This mechanism may elucidate fructose-induced chylomicron production in vivo.
PO327. THE IMPACT OF HDL CONCENTRATION ON EFFICIENCY OF LIPOPROTEIN LIPASE (LPL)-MEDIATED VLDL LIPOLYSIS IN PATIENTS WITH CHRONIC KIDNEY DISEASE Ewa Wieczorek1, Agnieszka Cwiklinska1, Monika Cackowska2, Agnieszka Kuchta1, Ewa Krol2, Barbara Kortas-Stempak1, Anna Gliwinska1, Kamil Jankowski1. 1 Medical University of Gdansk, Dabkowski1, Maciej Department of Clinical Chemistry, Gdansk, Poland; 2 Medical University of Gdansk, Chair & Clinic of Nephrology, Transplantology and Internal Diseases, Gdansk, Poland Aim: The impaired plasma VLDL lipolysis mediated by lipoprotein lipase (LPL) is one of the main cause of hypertriglyceridemia (HTG) in chronic kidney disease (CKD) patients. In our previous study we have found that the presence of HDL enhanced the efficiency of VLDL lipolysis (Cardiology 2015;132(supl.1):173-174). The aim of this study was to evaluate whether the impact of HDL on VLDL lipolysis efficiency is dependent on the HDL concentration. Methods: The sera were obtained from non-dialysis CKD patients of the Nephrology Clinic of Medical University of Gdansk. VLDL was isolated by ultracentrifugation, HDL by combined precipitation-ultracentrifugation procedure. VLDL was incubated with LPL (1 hour, 37 C, triglyceride (TG)VLDL:LPL 90:0.48 mg/dl, albumin 2%), in the absence or presence of increasing amounts of HDL (cholesterol (CH)-VLDL:CH-HDL 1:0, 1:0.1, 1:0.25, 1:0.5, 1:0.75, 1:1). After incubation, VLDL was separated by immunoprecipitation and the percentage of hydrolysed TG was calculated. Results: In the absence of HDL the percentage of hydrolysed TG-VLDL ranged from 44% to 80% (mean 67%) and was inversely correlated with the concentration of TG-VLDL (r¼-0.956, p¼0.01). The percentage of hydrolysed TG-VLDL increased with increasing amount of HDL (r¼0.983, p¼0.0004); the means were: 72%, 74%, 78%, 83% and 85% for CH-VLDL:CHHDL 1:0.1, 1:0.25, 1:0.5, 1:0.75, 1:1 ratios, respectively. The largest increase in the percentage of hydrolysed TG-VLDL under the influence of HDL was found for the sample with the highest concentration of TG-VLDL. Conclusions: HDL enhances the efficiency of VLDL lipolysis in a concentration-dependent manner. HDL deficiency may have impact on the exacerbation of HTG in CKD patients.
PO328. ANALYSIS OF SELECTIVELY ISOLATED CHYLOMICRON REMNANTS IN VARIOUS CONDITIONS Masuda Daisaku1, Takuya Kobayashi1, Masami Sairyo1, Takeshi Okada1, Masahiro Koseki1,2, Tohru Ohama1, 3, Makoto Nishida1, 2, Yasushi Sakata1, Shizuya Yamashita1, 4, 5. 1 Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Osaka, Japan; 2 Health Care Center, Osaka University, Osaka, Japan; 3 Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Osaka, Japan; 4 Department of Community Medicine, Osaka University Graduate School of Medicine, Osaka, Japan; 5 Rinku General Medical Center, Osaka, Japan Aim: Many studies suspected that postprandial increase in chylomicron remnants (CM-Rs) are known to be atherogenic. However, it is difficult to
examine the characteristics of CM-Rs in sera since they cannot be isolated selectively from other lipoproteins. We established the isolation method of CM-Rs using anti-apolipoprotein(apo) B-48 antibody. We evaluated the lipoprotein and proteomic profile of isolated CM-Rs in various conditions. Methods: Eight healthy volunteers and four diabetic patients were loaded with a high-fat diet and serum was collected after three hours. Lipoprotein profiles of serum samples, which were treated with the ultracentrifugation (d<1.019 g/mL) and immunoaffinity isolation using Sepharose beads containing anti-apoB-48 or anti-apoB-100 mAbs, were analyzed by an HPLC method. The protein profile of isolated lipoproteins were analyzed by shotgun proteomics and their postprandial changes were evaluated by the mouse intestinal lymph after the fat load. Results: Isolated CM-Rs from postprandial human serum contained lipoproteins with the size of large VLDL to small LDL. Apolipoproteins (apoB, A4, A5, M and clusterin), complements (C3, C4 etc) and paraoxonase 1 (PON-1) were contained in CM-Rs more that that in VLDL. Serum TG concentrations positively correlated with apoB-48, apoA5, apoC3 and PON-1. C3 and clusterin within the intestinal lymph were increased after the fat load, suggesting that they are important for the atherogenicity or inflammation during the postprandial period. Conclusions: CM-Rs contains more proteins involved in lipoprotein metabolism, inflammation and atherosclerosis than VLDL-Rs, postprandial increases of these proteins may be related to the enhanced atherogenesis in patients with increased remnant lipoproteins.
PO329. POSTPRANDIAL RESPONSE AFTER INTAKE OF SATURATED FAT COMPARED TO POLYUNSATURATED FAT IN SUBJECTS WITH FAMILIAL HYPERCHOLESTEROLEMIA AND NORMOLIPIDEMIC CONTROLS-A RANDOMIZED CONTROLLED TRIAL Linn Kristin Lie Øyri1, Patrik Hansson2, Martin Prøven Bogsrud1, Ingunn Narverud1, Geir Florholmen2, Lena Leder3, Marte Gjeitung Byfuglien3, Marit Bragelien Veierød4, Magne Thoresen4, Stine Marie Ulven2, Kirsten B. Holven1, 2. 1 Norwegian National Advisory Unit on Familial Hypercholesterolemia, Oslo University Hospital, OSLO, Norway; 2 Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, OSLO, Norway; 3 Mills DA, OSLO, Norway; 4 Department of Biostatistics, Institute for Basic Medical Sciences, University of Oslo, OSLO, Norway Aim: Postprandial hypertriglyceridemia is associated with increased risk of developing cardiovascular disease (CVD). We aimed to investigate the postprandial response in plasma triglycerides and very low-density lipoprotein (VLDL) after consumption of high-fat meals with different fat quality in subjects with familial hypercholesterolemia (FH) compared to healthy normolipidemic controls. Methods: A postprandial randomized controlled double-blinded crossover intervention study with two meals and two groups (FH subjects and controls) was performed. The meals consisted of two muffins, each containing 60 grams (70 E%) of fat, but different fat quality (either saturated fat (SFA, 42.8 E%) or polyunsaturated fat (PUFA, 39.2 E%)). The amount of monounsaturated fat was similar in the two muffins. Blood samples were taken at baseline (fasting) and 2, 4 and 6 hours after intake of the meals. Results: Thirteen subjects with FH (without current lipid-lowering treatment) and 14 controls were included, all aged 18 to 30 years and with BMI 18.5-30 kg/m2. Subjects with FH were characterized by higher plasma total- and LDL cholesterol and apolipoprotein (apo) B levels (P<0.001) than controls. No difference in BMI and body composition was seen. Plasma triglycerides increased in both groups after intake of both meals. Conclusions: Data on the postprandial triglyceride and apo B48 response after a meal enriched with SFA or PUFA will be presented. Moreover, we will specifically investigate the effects induced by meals with different fatty acid composition on lipoprotein subclasses, focusing specifically on VLDL subclasses.
PO330. RELATIONSHIP BETWEEN LDL SUBFRACTIONS AND POSTPRANDIAL LIPEMIA IN HEALTHY SUBJECTS
Abstracts / Atherosclerosis 263 (2017) e111ee282
Asim Orem1, Serap Ozer Yaman1, Buket Altinkaynak1, Birgul Vanizor Kural1, Fulya Balaban Yucesan1, Yahya Altinkaynak1, Cihan Orem2. 1 Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey; 2 Department of Cardiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey Aim: Postprandial lipemia, characterized by a rise in triglyceride-rich lipoproteins after eating. There are several lines of evidence suggesting that postprandial lipemia is associated with increased risk for cardiovascular disease. This study was aimed to determine relationship between postprandial lipemia and LDL subfractions as atherogenic lipoprotein particles in healthy subjects who divided to tertiles according to area under curve (AUC). Methods: Postprandial lipemia was evaluated by using standardized oral fat tolerance test (OFTT) in 96 healthy subjects (45 female and 51 male). AUC values were calculated by using triglyceride (TG) levels at the fasting state, 2nd, 4th and 6th hours after OFTT. Study group was categorized into tertiles according to AUC. LDL subfractions including small dense LDL (sdLDL) were separated by using the Lipoprint System (Quantimetrix). Levels of total cholesterol (TC), TG, LDL-C and HDL-C were determined with AU5800 autoanalyzer (Beckman Coulter) by using its original reagents. Results: When we compare the obtained values between bottom and top tertiles, sdLDL, was found to be significantly increased in the top tertile in both male and female subjects (p¼0.001, p¼0.035, respectively). AUC values showed a positive correlation with sdLDL in both genders (r¼0.58, p¼0.0001 and r¼0.49, p¼0.001, respectively). HDL-C level in the top tertile in both gender was found to be decreased while TG, TC and LDL-C levels were found to be increased (p<0.01). Conclusions: It was suggested that postprandial lipemia may be associated with increased atherogenic tendency with unfavourable changes in lipids and LDL subfractions.
PO331. CODING VARIANTS IDENTIFIED IN LMF1 GENE IN A COHORT OF HYPERCHYLOMICRONEMIC PATIENTS Marine Serveaux-Dancer1, 2, Christophe Marçais1, 2, Mathilde Di Filippo1, 3, re1, 4. 1 INSERM Unit Oriane Marmontel1, 3, Philippe Moulin1, 4, Sybil Charrie 2 1060 CarMen, Lyon 1 University, Lyon, France; Biochimie, Biologie Mol eculaire, Centre de Biologie Sud, Hospices Civils de Lyon, Lyon, France; 3 Biochimie Cardiovasculaire, Diagnostic Mol eculaire des Dyslipid emies, ed eration Centre de Biologie Est, Hospices Civils de Lyon, Lyon, France; 4 F ^le Est. Hospices Civils de Lyon, d'endocrinologie, Diab etologie, Nutrition. Po Lyon, France Aim: LMF1 (Lipase Maturation Factor 1) gene encodes for a protein involved Lipoprotein Lipase and Hepatic lipase maturation. Mutations on LMF1 leading to hyperchylomicronemia are rare in literature. A few polymorphisms have been described with poor functional studies. The aim of our study was to assess the frequency of LMF1 variants in a cohort of 410 hyperchylomicronemic patients with no deleterious mutations identified in LPL, APOA5, APOC2, GPIHBP1 genes. Methods: DHPLC and direct sequencing of LMLF1 gene. Comparison of variants frequencies in our cohort with EXAC population frequencies (European non finish cohort). In silico studies with Sift and Polyphen software to predict the functionality of the variants. Results: Our study identified a young girl as compound heterozygote for the Y464X mutation associated to the R451W variant. The Y464X mutation was previously described to be deleterious at homozygous state. The allele frequency of R451W variant is 0.61 % in our cohort of hyperchylomicronemic subjects vs 0.4 % in EXAC population (NS). We also identified four other variants (R351Q, R354W, R364Q, P562R), with a frequency > 1 % on our cohort. The allelic frequencies of R351Q and P562R were significantly increased compared to EXAC population. All variants were predicted to be deleterious in silico, except for R351Q. Conclusions: Our study confirms the rarity of LMF1 mutation in hyperchylomicronemia patient. Association studies in general population and functional studies are needed in order to decipher the involvement of LMF1 polymorphisms in hypertriglyceridemia.
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PO332. POSTPRANDIAL APOLIPOPROTEIN B-48 LEVEL IS INDEPENDENT OF INSULIN SECRETION IN PATIENTS WITH MODERATE OR SEVERE HYPERTRIGLYCERIDEMIA Rita Daclat1, Randa Bittar2, Joe-Elie Salem3, 6, Sandra Ricaldi4, Dominique Bonnefont-Rousselot3, Philippe Giral5, 6, Jean-Marc Lacorte1, 4, 6. 1 INSERM UMRS-ICAN 1166, Paris, France; 2 Biochimie M etabolique, Piti e-Salp^ etri ere, Paris, France; 3 CIC Paris Est, Piti eeSalp^ etri ere, Paris, France; 4 Biochimie Endocrinienne et Oncologique, Piti etabolisme, Piti e-Salp^ etri ere, Salp^ etri ere, Paris, France; 5 Endocrinologie M Paris, France; 6 UPMC, Paris, France Aim: Insulin-resistance and diabetes are usually associated with fasted hypertriglyceridemia (HTG) and increased secretion of intestinal triglyceride-rich lipoproteins (TRL). It was suggested that overproduction of intestinal TRL was due to insulin-resistance of the intestine. However, most of these results were obtained in subjects with normal or mild elevated triglyceridemia. In the present study we assessed the relation between insulin secretion and postprandial apoB-48 levels in hypertriglyceridemic patients. Methods: 39 patients with HTG were enrolled for postprandial exploration with standardized meal containing 800 kcal (lipids 35%). Fasting and postprandial level of lipid- and glucose-metabolism parameters were quantified before and hourly (6 hours) after the meal. Stratification of the subjects was done after calculation of HOMA-IR and two groups were distinguished: isolated hypertriglyceridemia (HTG, n¼23) and HTG with insulin-resistance (HTG-IR, n¼16). Results: At fasting, BMI, glucose and insulin levels were significantly higher in HTG-IR group than in HTG group but they were comparable with regards of triglycerides, apoB-100 and apoB-48. Ingestion of test-meal resulted in increased over the baseline of TG, apoB-48 but not apoB-100. However, there were no significant difference between HTG-IR and HTG groups concerning incremental TG, apoB-48 and postprandial apoB-100 whatever the postprandial time and the iAUC6h. Nevertheless, postprandial excursion of glucose and insulin are significantly higher in the HTG-IR group than in the HTG group as it is expected when insulin-resistance is involved. Conclusions: Postprandial elevation of TG and apoB-48 were independent of insulin secretion and insulin-resistance in patients with moderate or severe hypertriglyceridemia. PO333. TRIGLICERIDES AND HEART REMODELING Kakauridze, Tea Gamezardashvili, Lia Nana Nikolaishvili, Nona Xosroshvili, Xatuna Berinashvili, Zurab Leladze, Maia Bezhanishvili, Mindia Iobashvili. National Center of Surgery, Tbilisi, Georgia Aim: The aim of this study is to reveal relation between triglicerides and left ventricular remodeling types in patients with CHD . Methods: 120 patients were investigated: 75 with CHD. They were divided in groups Normal Geometry (NG), Concentric Hypertrophy (CH), Eccentric Hypertrophy (EH) and Concentric Remodeling (CR) by Ganau A. classification. Ultrasonografical Investigation: LV mass was calculated by linear measurements derived from 2D targeted M-mode. LVM estimated by the ASE-recommended formula (from LV linear dimensions): LVM¼ 0.8 {1.04[(LVIDd+ PWTd + SWTd)3 -(LVIDd)3]}+ 0.6 g; The indexation of LVM (g/m2) determined in accordance with BSA m2. For calculation of relative wall thickness (RWT) was used Formula: (2PWTd)/LVIDd. Obtained data was statistically analyzed by Microsoft Office SPSS e 11.5 program. Triglycerides (TG) was studied in blood serum using ”Janwey 4500” spectrometry Results: TG in NG e 117 mg/dl, CR -159.7mg/dl, CH -165.8mg/dl, EH 160.2mg/ dl, there was a following significant difference of TG levels between control and CHD (only three) groups: with CH - (p<0.001); EH (p<0.04); CR (p<0.05). We observed TG level statistically significant differences between: NG and CH (p<0.01); NG and EH (p<0.01); NG and CR (p<0.01) groups. Conclusions: The relation of TG in progression of CHD and LV remodeling is very important It let us to suggest that TG participate in LV remodeling
Abstracts / Atherosclerosis 263 (2017) e111ee282
group), DMEM supplemented 100mg/L glucose (Glucose group) or 100mg/ L fructose (Fructose group) for 1hour and protein and RNA was collected. mRNA expression was measured by RT-qPCR and protein expression was measured by western blotting. Results: Compared with control group, MTTP mRNA expression in glucose andreased fructose group was increased. MOGAT2 mRNA expression was increased only in fructose group. In protein expression, MTP and MGAT2 were increased in glucose and fructose group, and chREBP, SREBP-1 were upregulated only in fructose group. Conclusions: In caco-2 cells as enterocyte model, short-term fructose exposure increased lipogenic enzyme gene and protein expression such as MTP and MGAT2 by activating transcriptionfactors as chREBP and SREBP-1. This mechanism may elucidate fructose-induced chylomicron production in vivo.
PO327. THE IMPACT OF HDL CONCENTRATION ON EFFICIENCY OF LIPOPROTEIN LIPASE (LPL)-MEDIATED VLDL LIPOLYSIS IN PATIENTS WITH CHRONIC KIDNEY DISEASE Ewa Wieczorek1, Agnieszka Cwiklinska1, Monika Cackowska2, Agnieszka Kuchta1, Ewa Krol2, Barbara Kortas-Stempak1, Anna Gliwinska1, Kamil Jankowski1. 1 Medical University of Gdansk, Dabkowski1, Maciej Department of Clinical Chemistry, Gdansk, Poland; 2 Medical University of Gdansk, Chair & Clinic of Nephrology, Transplantology and Internal Diseases, Gdansk, Poland Aim: The impaired plasma VLDL lipolysis mediated by lipoprotein lipase (LPL) is one of the main cause of hypertriglyceridemia (HTG) in chronic kidney disease (CKD) patients. In our previous study we have found that the presence of HDL enhanced the efficiency of VLDL lipolysis (Cardiology 2015;132(supl.1):173-174). The aim of this study was to evaluate whether the impact of HDL on VLDL lipolysis efficiency is dependent on the HDL concentration. Methods: The sera were obtained from non-dialysis CKD patients of the Nephrology Clinic of Medical University of Gdansk. VLDL was isolated by ultracentrifugation, HDL by combined precipitation-ultracentrifugation procedure. VLDL was incubated with LPL (1 hour, 37 C, triglyceride (TG)VLDL:LPL 90:0.48 mg/dl, albumin 2%), in the absence or presence of increasing amounts of HDL (cholesterol (CH)-VLDL:CH-HDL 1:0, 1:0.1, 1:0.25, 1:0.5, 1:0.75, 1:1). After incubation, VLDL was separated by immunoprecipitation and the percentage of hydrolysed TG was calculated. Results: In the absence of HDL the percentage of hydrolysed TG-VLDL ranged from 44% to 80% (mean 67%) and was inversely correlated with the concentration of TG-VLDL (r¼-0.956, p¼0.01). The percentage of hydrolysed TG-VLDL increased with increasing amount of HDL (r¼0.983, p¼0.0004); the means were: 72%, 74%, 78%, 83% and 85% for CH-VLDL:CHHDL 1:0.1, 1:0.25, 1:0.5, 1:0.75, 1:1 ratios, respectively. The largest increase in the percentage of hydrolysed TG-VLDL under the influence of HDL was found for the sample with the highest concentration of TG-VLDL. Conclusions: HDL enhances the efficiency of VLDL lipolysis in a concentration-dependent manner. HDL deficiency may have impact on the exacerbation of HTG in CKD patients.
PO328. ANALYSIS OF SELECTIVELY ISOLATED CHYLOMICRON REMNANTS IN VARIOUS CONDITIONS Masuda Daisaku1, Takuya Kobayashi1, Masami Sairyo1, Takeshi Okada1, Masahiro Koseki1,2, Tohru Ohama1, 3, Makoto Nishida1, 2, Yasushi Sakata1, Shizuya Yamashita1, 4, 5. 1 Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine, Osaka, Japan; 2 Health Care Center, Osaka University, Osaka, Japan; 3 Department of Dental Anesthesiology, Osaka University Graduate School of Dentistry, Osaka, Japan; 4 Department of Community Medicine, Osaka University Graduate School of Medicine, Osaka, Japan; 5 Rinku General Medical Center, Osaka, Japan Aim: Many studies suspected that postprandial increase in chylomicron remnants (CM-Rs) are known to be atherogenic. However, it is difficult to
examine the characteristics of CM-Rs in sera since they cannot be isolated selectively from other lipoproteins. We established the isolation method of CM-Rs using anti-apolipoprotein(apo) B-48 antibody. We evaluated the lipoprotein and proteomic profile of isolated CM-Rs in various conditions. Methods: Eight healthy volunteers and four diabetic patients were loaded with a high-fat diet and serum was collected after three hours. Lipoprotein profiles of serum samples, which were treated with the ultracentrifugation (d<1.019 g/mL) and immunoaffinity isolation using Sepharose beads containing anti-apoB-48 or anti-apoB-100 mAbs, were analyzed by an HPLC method. The protein profile of isolated lipoproteins were analyzed by shotgun proteomics and their postprandial changes were evaluated by the mouse intestinal lymph after the fat load. Results: Isolated CM-Rs from postprandial human serum contained lipoproteins with the size of large VLDL to small LDL. Apolipoproteins (apoB, A4, A5, M and clusterin), complements (C3, C4 etc) and paraoxonase 1 (PON-1) were contained in CM-Rs more that that in VLDL. Serum TG concentrations positively correlated with apoB-48, apoA5, apoC3 and PON-1. C3 and clusterin within the intestinal lymph were increased after the fat load, suggesting that they are important for the atherogenicity or inflammation during the postprandial period. Conclusions: CM-Rs contains more proteins involved in lipoprotein metabolism, inflammation and atherosclerosis than VLDL-Rs, postprandial increases of these proteins may be related to the enhanced atherogenesis in patients with increased remnant lipoproteins.
PO329. POSTPRANDIAL RESPONSE AFTER INTAKE OF SATURATED FAT COMPARED TO POLYUNSATURATED FAT IN SUBJECTS WITH FAMILIAL HYPERCHOLESTEROLEMIA AND NORMOLIPIDEMIC CONTROLS-A RANDOMIZED CONTROLLED TRIAL Linn Kristin Lie Øyri1, Patrik Hansson2, Martin Prøven Bogsrud1, Ingunn Narverud1, Geir Florholmen2, Lena Leder3, Marte Gjeitung Byfuglien3, Marit Bragelien Veierød4, Magne Thoresen4, Stine Marie Ulven2, Kirsten B. Holven1, 2. 1 Norwegian National Advisory Unit on Familial Hypercholesterolemia, Oslo University Hospital, OSLO, Norway; 2 Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, OSLO, Norway; 3 Mills DA, OSLO, Norway; 4 Department of Biostatistics, Institute for Basic Medical Sciences, University of Oslo, OSLO, Norway Aim: Postprandial hypertriglyceridemia is associated with increased risk of developing cardiovascular disease (CVD). We aimed to investigate the postprandial response in plasma triglycerides and very low-density lipoprotein (VLDL) after consumption of high-fat meals with different fat quality in subjects with familial hypercholesterolemia (FH) compared to healthy normolipidemic controls. Methods: A postprandial randomized controlled double-blinded crossover intervention study with two meals and two groups (FH subjects and controls) was performed. The meals consisted of two muffins, each containing 60 grams (70 E%) of fat, but different fat quality (either saturated fat (SFA, 42.8 E%) or polyunsaturated fat (PUFA, 39.2 E%)). The amount of monounsaturated fat was similar in the two muffins. Blood samples were taken at baseline (fasting) and 2, 4 and 6 hours after intake of the meals. Results: Thirteen subjects with FH (without current lipid-lowering treatment) and 14 controls were included, all aged 18 to 30 years and with BMI 18.5-30 kg/m2. Subjects with FH were characterized by higher plasma total- and LDL cholesterol and apolipoprotein (apo) B levels (P<0.001) than controls. No difference in BMI and body composition was seen. Plasma triglycerides increased in both groups after intake of both meals. Conclusions: Data on the postprandial triglyceride and apo B48 response after a meal enriched with SFA or PUFA will be presented. Moreover, we will specifically investigate the effects induced by meals with different fatty acid composition on lipoprotein subclasses, focusing specifically on VLDL subclasses.
PO330. RELATIONSHIP BETWEEN LDL SUBFRACTIONS AND POSTPRANDIAL LIPEMIA IN HEALTHY SUBJECTS
Abstracts / Atherosclerosis 263 (2017) e111ee282
Asim Orem1, Serap Ozer Yaman1, Buket Altinkaynak1, Birgul Vanizor Kural1, Fulya Balaban Yucesan1, Yahya Altinkaynak1, Cihan Orem2. 1 Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey; 2 Department of Cardiology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey Aim: Postprandial lipemia, characterized by a rise in triglyceride-rich lipoproteins after eating. There are several lines of evidence suggesting that postprandial lipemia is associated with increased risk for cardiovascular disease. This study was aimed to determine relationship between postprandial lipemia and LDL subfractions as atherogenic lipoprotein particles in healthy subjects who divided to tertiles according to area under curve (AUC). Methods: Postprandial lipemia was evaluated by using standardized oral fat tolerance test (OFTT) in 96 healthy subjects (45 female and 51 male). AUC values were calculated by using triglyceride (TG) levels at the fasting state, 2nd, 4th and 6th hours after OFTT. Study group was categorized into tertiles according to AUC. LDL subfractions including small dense LDL (sdLDL) were separated by using the Lipoprint System (Quantimetrix). Levels of total cholesterol (TC), TG, LDL-C and HDL-C were determined with AU5800 autoanalyzer (Beckman Coulter) by using its original reagents. Results: When we compare the obtained values between bottom and top tertiles, sdLDL, was found to be significantly increased in the top tertile in both male and female subjects (p¼0.001, p¼0.035, respectively). AUC values showed a positive correlation with sdLDL in both genders (r¼0.58, p¼0.0001 and r¼0.49, p¼0.001, respectively). HDL-C level in the top tertile in both gender was found to be decreased while TG, TC and LDL-C levels were found to be increased (p<0.01). Conclusions: It was suggested that postprandial lipemia may be associated with increased atherogenic tendency with unfavourable changes in lipids and LDL subfractions.
PO331. CODING VARIANTS IDENTIFIED IN LMF1 GENE IN A COHORT OF HYPERCHYLOMICRONEMIC PATIENTS Marine Serveaux-Dancer1, 2, Christophe Marçais1, 2, Mathilde Di Filippo1, 3, re1, 4. 1 INSERM Unit Oriane Marmontel1, 3, Philippe Moulin1, 4, Sybil Charrie 2 1060 CarMen, Lyon 1 University, Lyon, France; Biochimie, Biologie Mol eculaire, Centre de Biologie Sud, Hospices Civils de Lyon, Lyon, France; 3 Biochimie Cardiovasculaire, Diagnostic Mol eculaire des Dyslipid emies, ed eration Centre de Biologie Est, Hospices Civils de Lyon, Lyon, France; 4 F ^le Est. Hospices Civils de Lyon, d'endocrinologie, Diab etologie, Nutrition. Po Lyon, France Aim: LMF1 (Lipase Maturation Factor 1) gene encodes for a protein involved Lipoprotein Lipase and Hepatic lipase maturation. Mutations on LMF1 leading to hyperchylomicronemia are rare in literature. A few polymorphisms have been described with poor functional studies. The aim of our study was to assess the frequency of LMF1 variants in a cohort of 410 hyperchylomicronemic patients with no deleterious mutations identified in LPL, APOA5, APOC2, GPIHBP1 genes. Methods: DHPLC and direct sequencing of LMLF1 gene. Comparison of variants frequencies in our cohort with EXAC population frequencies (European non finish cohort). In silico studies with Sift and Polyphen software to predict the functionality of the variants. Results: Our study identified a young girl as compound heterozygote for the Y464X mutation associated to the R451W variant. The Y464X mutation was previously described to be deleterious at homozygous state. The allele frequency of R451W variant is 0.61 % in our cohort of hyperchylomicronemic subjects vs 0.4 % in EXAC population (NS). We also identified four other variants (R351Q, R354W, R364Q, P562R), with a frequency > 1 % on our cohort. The allelic frequencies of R351Q and P562R were significantly increased compared to EXAC population. All variants were predicted to be deleterious in silico, except for R351Q. Conclusions: Our study confirms the rarity of LMF1 mutation in hyperchylomicronemia patient. Association studies in general population and functional studies are needed in order to decipher the involvement of LMF1 polymorphisms in hypertriglyceridemia.
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PO332. POSTPRANDIAL APOLIPOPROTEIN B-48 LEVEL IS INDEPENDENT OF INSULIN SECRETION IN PATIENTS WITH MODERATE OR SEVERE HYPERTRIGLYCERIDEMIA Rita Daclat1, Randa Bittar2, Joe-Elie Salem3, 6, Sandra Ricaldi4, Dominique Bonnefont-Rousselot3, Philippe Giral5, 6, Jean-Marc Lacorte1, 4, 6. 1 INSERM UMRS-ICAN 1166, Paris, France; 2 Biochimie M etabolique, Piti e-Salp^ etri ere, Paris, France; 3 CIC Paris Est, Piti eeSalp^ etri ere, Paris, France; 4 Biochimie Endocrinienne et Oncologique, Piti etabolisme, Piti e-Salp^ etri ere, Salp^ etri ere, Paris, France; 5 Endocrinologie M Paris, France; 6 UPMC, Paris, France Aim: Insulin-resistance and diabetes are usually associated with fasted hypertriglyceridemia (HTG) and increased secretion of intestinal triglyceride-rich lipoproteins (TRL). It was suggested that overproduction of intestinal TRL was due to insulin-resistance of the intestine. However, most of these results were obtained in subjects with normal or mild elevated triglyceridemia. In the present study we assessed the relation between insulin secretion and postprandial apoB-48 levels in hypertriglyceridemic patients. Methods: 39 patients with HTG were enrolled for postprandial exploration with standardized meal containing 800 kcal (lipids 35%). Fasting and postprandial level of lipid- and glucose-metabolism parameters were quantified before and hourly (6 hours) after the meal. Stratification of the subjects was done after calculation of HOMA-IR and two groups were distinguished: isolated hypertriglyceridemia (HTG, n¼23) and HTG with insulin-resistance (HTG-IR, n¼16). Results: At fasting, BMI, glucose and insulin levels were significantly higher in HTG-IR group than in HTG group but they were comparable with regards of triglycerides, apoB-100 and apoB-48. Ingestion of test-meal resulted in increased over the baseline of TG, apoB-48 but not apoB-100. However, there were no significant difference between HTG-IR and HTG groups concerning incremental TG, apoB-48 and postprandial apoB-100 whatever the postprandial time and the iAUC6h. Nevertheless, postprandial excursion of glucose and insulin are significantly higher in the HTG-IR group than in the HTG group as it is expected when insulin-resistance is involved. Conclusions: Postprandial elevation of TG and apoB-48 were independent of insulin secretion and insulin-resistance in patients with moderate or severe hypertriglyceridemia. PO333. TRIGLICERIDES AND HEART REMODELING Kakauridze, Tea Gamezardashvili, Lia Nana Nikolaishvili, Nona Xosroshvili, Xatuna Berinashvili, Zurab Leladze, Maia Bezhanishvili, Mindia Iobashvili. National Center of Surgery, Tbilisi, Georgia Aim: The aim of this study is to reveal relation between triglicerides and left ventricular remodeling types in patients with CHD . Methods: 120 patients were investigated: 75 with CHD. They were divided in groups Normal Geometry (NG), Concentric Hypertrophy (CH), Eccentric Hypertrophy (EH) and Concentric Remodeling (CR) by Ganau A. classification. Ultrasonografical Investigation: LV mass was calculated by linear measurements derived from 2D targeted M-mode. LVM estimated by the ASE-recommended formula (from LV linear dimensions): LVM¼ 0.8 {1.04[(LVIDd+ PWTd + SWTd)3 -(LVIDd)3]}+ 0.6 g; The indexation of LVM (g/m2) determined in accordance with BSA m2. For calculation of relative wall thickness (RWT) was used Formula: (2PWTd)/LVIDd. Obtained data was statistically analyzed by Microsoft Office SPSS e 11.5 program. Triglycerides (TG) was studied in blood serum using ”Janwey 4500” spectrometry Results: TG in NG e 117 mg/dl, CR -159.7mg/dl, CH -165.8mg/dl, EH 160.2mg/ dl, there was a following significant difference of TG levels between control and CHD (only three) groups: with CH - (p<0.001); EH (p<0.04); CR (p<0.05). We observed TG level statistically significant differences between: NG and CH (p<0.01); NG and EH (p<0.01); NG and CR (p<0.01) groups. Conclusions: The relation of TG in progression of CHD and LV remodeling is very important It let us to suggest that TG participate in LV remodeling