Relationship of high energy phosphate concentrations and oxygen consumption in isolated perfused rat hearts

18 RELATIONSHIP OF HIGH ENERGY PHOSPHATE CONCENTRATIONS AND OXYGEN CONSUMPTION IN ISOLATED PERFUSED RAT HEARTS. J. Giesen, H. Kammermeier, Abt. Physiologie, Med. Fak., RWTH Aachen, W. Germany. In isolated mitochondria (state 4/3) respiration depends linearly on the phosphorylation potential (log (ATP)/(ADP) (Pi)) of the medium. Experiments were performed to look for similar relationship in the intact myocardium. The following corrections for metabolite binding or compartmentation were carried out to obtain sarcoplasmic concentrations of the respective metabolites. I. Correction for constant and proportional values according to literature. II. Correction for ATP and Pi as I., calculation of ADP from CPK equilibrium assuming constant pH. III. Correction as II. assuming respiration dependent changes of pH according to literature. IV. Calculation of Pi distribution correlated to Hf distribution according to literature. For I to IV increasing coefficients of correlation (0.5 to 0.97) were obtained indicating that in the intact heart mitochondrial respiration is regulated in the same way as in mitochondrial suspension by the phosphorylation potential or indirectly because of CPK equilibrium by the ratio (Cr).(Pi)/(CrP).(H+).KcpK. This close relationship enables to charactize disturbances of myocardial energy metabolism more in detail. Supported

by Deutsche

Forschungsgemeinschaft

Ka 33714-5

MPOBIOCHEMISTRY OF PROTEINASE SPECIFICITY PATTERNS IN NORMAL AND INFARCT-DAMAGED HEART MUSCLE TISSUES. M. Gottwik*, J.H. Wissler, H.H. Klein, J. Schaper and W. Schaper, Kerckhoff-Klinik* and Max-Planck-Institut fiir Exp. Kardiologie, D-6350, Bad Nauheim, Germany. For evaluation and differentiation of the role and action sequence of lysosomal functions and proteinase activity regulation within tissue injury, ininfarction, we have deterflammation and tissue repair processes in m ocardial mined and urified some selected aminopepti 4 ase, elastase, trypsin-like, chymctr psin-li 3 e and cathepsin proteinase specificities in normal and infarct-damage 5; heart muscle tissue sections. Infarcts have been produced in the anterior wall of doa heart bv occlusion of the left anterior descending branch of the rocedure. After remoleft coronary arter9 with a transfemoral catheterization non-inval. the hearts were divided into infarcted (anterior wal f ) and several