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Relative inability of allogeneic endothelia to stimulate human helper T cells as measured by limiting dilution analysis
Abstracts
47
#20O 6.2
#201 7.3
RACTAL DZFFERENCES XN THE ASSOCIATXON OF DR2/DQ~I ~XTH NARCOLEPSY ~ASED ON SEROLOGXCAL A~D DNA TYPXNG. ~ ~atson, C Jones, I" MiP~lail, ML Tseng, RCP Go, T. Woolley, V ~o~:en, L Herman, end ~ _ ~ L ~ , Schools of Medicine and Public Health, University o~ Alabama at B~mingham, Birmingham, AI. Narcolepsy is a sleep d~sorder characterized by excessive daytime sleepiness and abnor~litie~ Of rapid aye m o v ~ m t . Cataplexy and other ancillary symptoms may also be present. Strong association between D R 2 / D ~ I and narcolepsy emon~ Japanese and some Caucasian ~opulations, up t O 100%, ha~ been reported. Serological typing and HLA class XX s e ~ e n c e specific oligonucleotide were used t o study t~e genetic etiology oE narcolepsy in blacks and w h i t e . Patients ~ r ~ stratified based on family h i s t o r y , r a ~ , and cataplexy + / - . Of 71 subjects studied, 2 patients with cataple~y and 23 patients without oataplexy r e p o r t ~ an affected first d ~ r e e relative. 13 white and 8 black narcoleptics without cataple~y were DR2 ( - ) . 8 whites and 1 black patient with cataple~y ~era DQwI(-). 1 black and 3 white patients with cataplexy and 5 white patients without oataple~- were DR2 (-} and D~wl(-} by both serology and DNA testing. ~n narcoleptics were compared to controls no significant difference in the frequency Of DR2(+) and Dc~l(+} was f ~ (odds r a t i o 1. 082, 0.788 ; 1.531, 1.790; with & ~ithout c a t a p l ~ y respectively Eor DR2 end D q ~ . Xdentificatlon o f narcoleptics who were both DR2 and DQwl negative, both ~ith and W i t h o u t cataplexy, using two different typing methodologies suggests that DR2 is not the actual ~ene predisposing to narcolepsy in either blacks or whites.
RELATIVE INABiL|TY OF ALLOGENEIC E@THEUA TO STIMULATE HUMAN HELPER T CELLS AS MEASURED BY LiMiTiNG ~DtLUTION ANALYSIS. P.=.t~D~, D ~ , and C Oro¢~, ~ of S~rg~t, The Oh~ State ~ Hesper, ~ , OH. We ~ us~i LDA to ~ the r ~ ' ~ of h u ~ p ~ i p h ~ ~ T that can produce IL-2 in rt~po~e to rest~g ~ human ~ vein ~ o t h e ~ c~s (HUVEC), to ~ ~FN)~ HUVEC, HUVEC s t i l e
modQstnumbers of ~
T c~%s(
giFN-~ HUVEC ~ ~ (~proxi~t~y 1./7OO), and ~ Me ~
~
~
!/8000),
more T csHs 1/200 T
g | F N - t r ~ HUVEC are C ~ + , wh~ees aN of the HTL ~ to ~ltr~tsd HUV~C are CD~- (CD~+?). C y t o ~ ~ that g|FN- t ~ t e d HUVEC ~ dLspC~y~ MHC C/ass U antigens than Mo. Thesest~es den~strat~ that HUV~C can ~ st~u~e HI=L, ~ after treatment w~th gtFN, b~ never as eff~cttv~ as Mo. Further, they ~ - a t e that diffract T c ~ ~ respond to e n c Y - - , c~penc~ on the de~'ee of ~ ~ activation.
47
#20O 6.2
#201 7.3
RACTAL DZFFERENCES XN THE ASSOCIATXON OF DR2/DQ~I ~XTH NARCOLEPSY ~ASED ON SEROLOGXCAL A~D DNA TYPXNG. ~ ~atson, C Jones, I" MiP~lail, ML Tseng, RCP Go, T. Woolley, V ~o~:en, L Herman, end ~ _ ~ L ~ , Schools of Medicine and Public Health, University o~ Alabama at B~mingham, Birmingham, AI. Narcolepsy is a sleep d~sorder characterized by excessive daytime sleepiness and abnor~litie~ Of rapid aye m o v ~ m t . Cataplexy and other ancillary symptoms may also be present. Strong association between D R 2 / D ~ I and narcolepsy emon~ Japanese and some Caucasian ~opulations, up t O 100%, ha~ been reported. Serological typing and HLA class XX s e ~ e n c e specific oligonucleotide were used t o study t~e genetic etiology oE narcolepsy in blacks and w h i t e . Patients ~ r ~ stratified based on family h i s t o r y , r a ~ , and cataplexy + / - . Of 71 subjects studied, 2 patients with cataple~y and 23 patients without oataplexy r e p o r t ~ an affected first d ~ r e e relative. 13 white and 8 black narcoleptics without cataple~y were DR2 ( - ) . 8 whites and 1 black patient with cataple~y ~era DQwI(-). 1 black and 3 white patients with cataplexy and 5 white patients without oataple~- were DR2 (-} and D~wl(-} by both serology and DNA testing. ~n narcoleptics were compared to controls no significant difference in the frequency Of DR2(+) and Dc~l(+} was f ~ (odds r a t i o 1. 082, 0.788 ; 1.531, 1.790; with & ~ithout c a t a p l ~ y respectively Eor DR2 end D q ~ . Xdentificatlon o f narcoleptics who were both DR2 and DQwl negative, both ~ith and W i t h o u t cataplexy, using two different typing methodologies suggests that DR2 is not the actual ~ene predisposing to narcolepsy in either blacks or whites.
RELATIVE INABiL|TY OF ALLOGENEIC E@THEUA TO STIMULATE HUMAN HELPER T CELLS AS MEASURED BY LiMiTiNG ~DtLUTION ANALYSIS. P.=.t~D~, D ~ , and C Oro¢~, ~ of S~rg~t, The Oh~ State ~ Hesper, ~ , OH. We ~ us~i LDA to ~ the r ~ ' ~ of h u ~ p ~ i p h ~ ~ T that can produce IL-2 in rt~po~e to rest~g ~ human ~ vein ~ o t h e ~ c~s (HUVEC), to ~ ~FN)~ HUVEC, HUVEC s t i l e
modQstnumbers of ~
T c~%s(
giFN-~ HUVEC ~ ~ (~proxi~t~y 1./7OO), and ~ Me ~
~
~
!/8000),
more T csHs 1/200 T
g | F N - t r ~ HUVEC are C ~ + , wh~ees aN of the HTL ~ to ~ltr~tsd HUV~C are CD~- (CD~+?). C y t o ~ ~ that g|FN- t ~ t e d HUVEC ~ dLspC~y~ MHC C/ass U antigens than Mo. Thesest~es den~strat~ that HUV~C can ~ st~u~e HI=L, ~ after treatment w~th gtFN, b~ never as eff~cttv~ as Mo. Further, they ~ - a t e that diffract T c ~ ~ respond to e n c Y - - , c~penc~ on the de~'ee of ~ ~ activation.