- Email: [email protected]
Reproducibility and validation of translational pig model of myocardial infarction by the ischemic preconditioning: Effect on intramyocardial hemorrhage
216 dria, especially by increasing IP3-R response to ATP stimulation in Ca2+ hotspots (in the outer mitochondrial membrane). In addition, puromycin pre-treatment increases the mitochondrial ATP content. Finally, TLC modulation during HR sequence show a decrease of cell death. Conclusion Pharmacological modulations of TLC could be an effective cardioprotection strategy, by regulating Ca2+ exchanges between ER and mitochondria, to reduce cell death after a MI. Disclosure of interest The authors declare that they have no competing interest. https://doi.org/10.1016/j.acvdsp.2019.02.074 534
Stable Extracranial ICA Patency after Mechanical Thrombectomy for Ischemic Stroke Patients with Tandem Occlusions: Major determinant of favorable outcome T. Personnic 1 , N. Bricout 1 , H. Henon 2 , M. Bretzner 1 , G. Lemesle 3,∗ , J.P. Pruvo 1 1 Service de neuroradiologie, CHU de Lille 2 Service de neurologie, CHU de Lille 3 Usic et centre hémodynamique, Institut Coeur Poumon, CHRU de Lille, Lille, France ∗ Corresponding author. E-mail address: gilles [email protected] (G. Lemesle) Background Mechanical thrombectomy (MT) for acute ischemic stroke (AIS) with tandem occlusion is efficient, but optimal management of the extracranial occlusive component remains controversial. We investigated the association between extracranial internal carotid artery (ICA) patency on day 1 follow-up MRAngiography and clinical outcome after MT in AIS patients with tandem occlusion. Methods Consecutive AIS patients with tandem occlusion were identified from a hospital-based prospective registry from January 2011 to January 2017. Baseline clinical-imaging characteristics, angiographic outcomes and day 1 follow-up imaging findings (including extracranial ICA patency as assessed by MR-Angiography) were analyzed with regards to their associations with 3-month modified Rankin scale (mRS) scores. Favorable outcome was defined as a mRS score 0—2 at 3 months. Results Out of 594 AIS patients treated with MT during the study period, 83 met inclusion criteria (69.9% male, aged 62.3 ± 12.3). Mean NIHSS was 17.9 ± 4.8 and median ASPECTS was 7. Successful re-canalization (mTICI 2b/3) was achieved in 61.5%. Extracranial ICA was patent in 37/83 patients (44.6%) at day 1, more frequently in those with prior IV thrombolysis (P = 0.035) or with cervical re-vascularization procedure (balloon angioplasty or stenting, P = 0.034). Favorable 3-month functional outcome was more frequent in patients with patent extracranial ICA at day 1 (64.9% vs 21.7%, P < 0.0001) independently of intracranial recanalization success. No difference was found in terms of sICH (13.5% vs 10.9%, P = 0.75) or 3-month mortality rate (8.1% vs 17.4%, P = 0.33). Conclusion This study highlights the importance of stable extracranial ICA patency in determining outcome of AIS patients with tandem occlusions. Randomized studies are needed. Disclosure of interest The authors declare that they have no competing interest. https://doi.org/10.1016/j.acvdsp.2019.02.075
Topic 10—Ischemia-reperfusion, infarction, stroke—C 264
Reproducibility and validation of translational pig model of myocardial infarction by the ischemic preconditioning: Effect on intramyocardial hemorrhage M. Isabelle ∗ , A. Helbert , L. Lesage , M. Lacombe , C. Mauclair , J. Melka , N. Villeneuve URDCV, Institut de recherches Servier, Suresnes, France ∗ Corresponding author. E-mail address: [email protected] (M. Isabelle) Introduction Reproducibility and validation of a preclinical model are two major corner stones to improve translation of preclinical results to the clinic [1]. Here, we propose to study the reproducibility of a pig model of acute myocardial infarction (MI) recommended by the CAESAR Labs [2], its validation using ischemic preconditioning (IPC) and to investigate the effects of IPC on the intramyocardial hemorrhage (IMH) phenomenon which appears during the reperfusion phase. Methods MI was induced in farm pig (25 kg) during a 60 min LAD occlusion (MICAESAR). Either IPCCAESAR (3 cycles of 5 min occlusion followed by 10 min reperfusion) or internal protocol IPCIdRS (2 cycles of 10 min occlusion followed by 10 min reperfusion) was performed prior to MI induction. After three days of reperfusion, area at risk (AAR), infarct size (IS) and IMH size were measured by both gravimetric and planimetric analysis. Data are reported as mean ± SD (n). Results IR induced reproducible infarct size (55% ± 6; n = 12), similar to those reported by CAESAR consortium (58% ± 14; n = 13). Surprisingly, IPCCAESAR protocol did not significantly reduce IS (44% ± 18; n = 8) while significant beneficial effects of internal IPC protocol were demonstrated (38% ± 16; n = 8). Moreover, internal protocol IPCIdRS reduced significantly IMH expressed as a percentage of AAR but not when expressed as a percentage of IS. Conclusions CAESAR protocol induced highly reproducible postischemic infarct size in farm pig. However, the cardio-protective effect of the recommended IPC sequence appeared to be more fluctuant and dependent of the lab. The observed reduction of the IMH size by internal IPC protocol is strongly dependent on the infarct size reduction. Our results do not indicate that IPC could reduce the IMH phenomenon per se. Disclosure of interest The authors declare that they have no competing interest. References [1] Merry L. Lindsey et al. Am J Physiol Heart Circ Physiol 314: H812H838, 2018. [2] Steven P. Jones et al. Circ Res.2015.116. 572-586. https://doi.org/10.1016/j.acvdsp.2019.02.076 516
Serum cholesterol efflux capacity and mortality in patients with acute myocardial infarction M. Guerin 1,∗ , J. Silvain 1,2 , J. Gall 1 , M. Darabi 1 , E. Frisdal 1 , M. Hauguel-Moreau 2 , M. Zeitouni 2 , M. Kerneis 1,2 , B. Lattuca 1,2 , D. Brugier 2 , J.P. Collet 1,2 , P. Lesnik 1 , G. Montalescot 1,2 1 UMRS1166 ICAN, Inserm U1166 ICAN 2 Action Study Group Institut de Cardiologie, Sorbonne Université, Paris, France ∗ Corresponding author. E-mail address: [email protected] (M. Guerin) Introduction Serum cholesterol efflux capacity, a biomarker of the early step of the reverse cholesterol transport, has been associated with atherosclerosis independently of high-density lipoprotein (HDL) cholesterol level.
Stable Extracranial ICA Patency after Mechanical Thrombectomy for Ischemic Stroke Patients with Tandem Occlusions: Major determinant of favorable outcome T. Personnic 1 , N. Bricout 1 , H. Henon 2 , M. Bretzner 1 , G. Lemesle 3,∗ , J.P. Pruvo 1 1 Service de neuroradiologie, CHU de Lille 2 Service de neurologie, CHU de Lille 3 Usic et centre hémodynamique, Institut Coeur Poumon, CHRU de Lille, Lille, France ∗ Corresponding author. E-mail address: gilles [email protected] (G. Lemesle) Background Mechanical thrombectomy (MT) for acute ischemic stroke (AIS) with tandem occlusion is efficient, but optimal management of the extracranial occlusive component remains controversial. We investigated the association between extracranial internal carotid artery (ICA) patency on day 1 follow-up MRAngiography and clinical outcome after MT in AIS patients with tandem occlusion. Methods Consecutive AIS patients with tandem occlusion were identified from a hospital-based prospective registry from January 2011 to January 2017. Baseline clinical-imaging characteristics, angiographic outcomes and day 1 follow-up imaging findings (including extracranial ICA patency as assessed by MR-Angiography) were analyzed with regards to their associations with 3-month modified Rankin scale (mRS) scores. Favorable outcome was defined as a mRS score 0—2 at 3 months. Results Out of 594 AIS patients treated with MT during the study period, 83 met inclusion criteria (69.9% male, aged 62.3 ± 12.3). Mean NIHSS was 17.9 ± 4.8 and median ASPECTS was 7. Successful re-canalization (mTICI 2b/3) was achieved in 61.5%. Extracranial ICA was patent in 37/83 patients (44.6%) at day 1, more frequently in those with prior IV thrombolysis (P = 0.035) or with cervical re-vascularization procedure (balloon angioplasty or stenting, P = 0.034). Favorable 3-month functional outcome was more frequent in patients with patent extracranial ICA at day 1 (64.9% vs 21.7%, P < 0.0001) independently of intracranial recanalization success. No difference was found in terms of sICH (13.5% vs 10.9%, P = 0.75) or 3-month mortality rate (8.1% vs 17.4%, P = 0.33). Conclusion This study highlights the importance of stable extracranial ICA patency in determining outcome of AIS patients with tandem occlusions. Randomized studies are needed. Disclosure of interest The authors declare that they have no competing interest. https://doi.org/10.1016/j.acvdsp.2019.02.075
Topic 10—Ischemia-reperfusion, infarction, stroke—C 264
Reproducibility and validation of translational pig model of myocardial infarction by the ischemic preconditioning: Effect on intramyocardial hemorrhage M. Isabelle ∗ , A. Helbert , L. Lesage , M. Lacombe , C. Mauclair , J. Melka , N. Villeneuve URDCV, Institut de recherches Servier, Suresnes, France ∗ Corresponding author. E-mail address: [email protected] (M. Isabelle) Introduction Reproducibility and validation of a preclinical model are two major corner stones to improve translation of preclinical results to the clinic [1]. Here, we propose to study the reproducibility of a pig model of acute myocardial infarction (MI) recommended by the CAESAR Labs [2], its validation using ischemic preconditioning (IPC) and to investigate the effects of IPC on the intramyocardial hemorrhage (IMH) phenomenon which appears during the reperfusion phase. Methods MI was induced in farm pig (25 kg) during a 60 min LAD occlusion (MICAESAR). Either IPCCAESAR (3 cycles of 5 min occlusion followed by 10 min reperfusion) or internal protocol IPCIdRS (2 cycles of 10 min occlusion followed by 10 min reperfusion) was performed prior to MI induction. After three days of reperfusion, area at risk (AAR), infarct size (IS) and IMH size were measured by both gravimetric and planimetric analysis. Data are reported as mean ± SD (n). Results IR induced reproducible infarct size (55% ± 6; n = 12), similar to those reported by CAESAR consortium (58% ± 14; n = 13). Surprisingly, IPCCAESAR protocol did not significantly reduce IS (44% ± 18; n = 8) while significant beneficial effects of internal IPC protocol were demonstrated (38% ± 16; n = 8). Moreover, internal protocol IPCIdRS reduced significantly IMH expressed as a percentage of AAR but not when expressed as a percentage of IS. Conclusions CAESAR protocol induced highly reproducible postischemic infarct size in farm pig. However, the cardio-protective effect of the recommended IPC sequence appeared to be more fluctuant and dependent of the lab. The observed reduction of the IMH size by internal IPC protocol is strongly dependent on the infarct size reduction. Our results do not indicate that IPC could reduce the IMH phenomenon per se. Disclosure of interest The authors declare that they have no competing interest. References [1] Merry L. Lindsey et al. Am J Physiol Heart Circ Physiol 314: H812H838, 2018. [2] Steven P. Jones et al. Circ Res.2015.116. 572-586. https://doi.org/10.1016/j.acvdsp.2019.02.076 516
Serum cholesterol efflux capacity and mortality in patients with acute myocardial infarction M. Guerin 1,∗ , J. Silvain 1,2 , J. Gall 1 , M. Darabi 1 , E. Frisdal 1 , M. Hauguel-Moreau 2 , M. Zeitouni 2 , M. Kerneis 1,2 , B. Lattuca 1,2 , D. Brugier 2 , J.P. Collet 1,2 , P. Lesnik 1 , G. Montalescot 1,2 1 UMRS1166 ICAN, Inserm U1166 ICAN 2 Action Study Group Institut de Cardiologie, Sorbonne Université, Paris, France ∗ Corresponding author. E-mail address: [email protected] (M. Guerin) Introduction Serum cholesterol efflux capacity, a biomarker of the early step of the reverse cholesterol transport, has been associated with atherosclerosis independently of high-density lipoprotein (HDL) cholesterol level.