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Research progress on chemical composition, biological activity, and quality control of Radix Platycodi
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Abstracts / European Journal of Integrative Medicine 6 (2014) 686–745
Research progress on chemical composition, biological activity, and quality control of Radix Platycodi Ye Yunyun 1,2 , Han Lu 1,2,3 , Zhang Yan 1,2 , Sun Jiayou 1,2 , Zhou Li 1 , Bai Changcai 1,2,3,4,5,∗ 1 Ningxia
Medical University Pharmacy College, Yinchuan 750004, China 2 Ningxia Engineering & Technology Research Center for Modernization of Hui Medicine, Yinchuan, 750004, China 3 Ningxia Collaborative Innovation Center for Hui Medicine, Yinchuan 750004, China 4 Key Laboratory of Hui Medicine Modernization, Ministry of Education, Yinchuan 750004, China 5 Ningxia Medicine Research Institute, Yinchuan 750004, China E-mail address: [email protected] (B. Changcai). Introduction: To provide a basis for further research and utilization of Radix Platycodi, the chemical constituents, biological activity, and quality control were reviewed. Methods: By literature reviewing and compilation of relevant data, literature on its chemical composition, biological activity and quality control studies were collected and analyzed. Results: The present chemical study of Platycodon grandiflorum were more focused on the composition of roots and less on other parts; its pharmacological activity mainly confined to the pharmacological activity of its water extract or total saponins, and reports on pharmacological activity of compounds which separated from P. grandiflorum were less. Conclusions: Future research on Radix Platycodi should focus in-depth on the chemical composition, and multiaspect biological activity and develop practical quality control indicators, which will provide scientific reference for its further study and comprehensive ultilization. http://dx.doi.org/10.1016/j.eujim.2014.09.088 New triterpenic acids and alkaloids from Uncaria rhynchophylla and their CID studies by use of an LTQ-Orbitrap Velos Pro mass spectrometer Wen-zhi Yang, Yi-bei Zhang, Wan-ying Wu, Min Yang, De-an Guo ∗ National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, PR China E-mail address: [email protected] (D.-a. Guo). Introduction: The genus Uncaria (Rubiaceae) is a crucial member of TCM family that has benefited human health
since ancient times. Alkaloids and triterpenes are two important groups of bioactive ingredients responsible for the therapeutic effects of Uncaria-derived traditional medicines. In the Chinese Pharmacopoeia (2010 edition), the stems and hooks of Uncaria rhynchophylla, U. macrophylla, U. hirsute, U. sinensis, and U. sessilifructus are collected as Uncariae Ramulus Cum Uncis (Gou-Teng) and indicated to treat dizziness, hyperpyrexia, and convulsion, etc. Notably, U. rhynchophylla and U. tomentosa are also considered as the promising agents to remedy Alzheimer’s disease and Parkinson disease, respectively. Previous phytochemical studies on U. rhynchophylla have resulted in the isolation of indole alkaloids, triterpenic acids, and phenolic compounds. Methods: With the view of exploring new bioactive chemicals in U. rhynchophylla, we conducted a systematic phytochemical study on the ethanol extracts of U. rhynchophylla. Results: Finally, 26 compounds, involving five new triterpenic acids, two new alkaloids, as well as 19 known compounds, were obtained. Of these, three triterpenic acids exhibited weak inhibitory effects on LPS-induced NO production in RAW264.7 cells. The CID-ESI-MSn behaviors of these pure compounds were comparatively studied by utility of an LTQ-Orbitrap Velos Pro mass spectrometer. 19-OH substituted oleanane triterpenic acids were prone to eliminate CH2 O3 (62 Da), whereas those ursane-type encompassing 19-OH were featured by preferred cleavage of H2 O (18 Da) while performing the negative CIDMS/MS fragmentation. In the case of alkaloids analyzed in the positive mode, diagnostic ions and characteristic cleavages were found that could assist in differentiating tetracyclic monotriterpene indole, tetracyclic monotriterpene oxidation indole, and tetracyclic monotriterpene indole N-oxide. Conclusions: A qualitative analysis strategy for characterizing the chemicals in Uncaria species is proposed, based on which 123 compounds in total were identified or tentatively characterized from four self-collected Uncaria plants. http://dx.doi.org/10.1016/j.eujim.2014.09.089 Antitussive and expectorant constituents of Reineckiacarnea (Andr.) Kunth Jun Yin ∗ , Yichun Wang, Ting Huang, Zhihui Liu, Na Han School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China E-mail address: [email protected] (J. Yin). Introduction: Reineckiacarnea (Andr.) Kunth, a kind of traditional ethnic drug, mainly is distributed in Guizhou and Yunnan provinces of southern China, whose aerial part is used for preventing cough, eliminating phlegm, rheumatism disease treatment and hepatitis. We aim to illustrate the active constituents of the aqueous extract of aerial part of Reineckiacarnea by bioassay guided isolation. Methods: The aqueous extract of Reineckiacarnea Herba was subjected to chromatographic separation on Diaion-101
Abstracts / European Journal of Integrative Medicine 6 (2014) 686–745
Research progress on chemical composition, biological activity, and quality control of Radix Platycodi Ye Yunyun 1,2 , Han Lu 1,2,3 , Zhang Yan 1,2 , Sun Jiayou 1,2 , Zhou Li 1 , Bai Changcai 1,2,3,4,5,∗ 1 Ningxia
Medical University Pharmacy College, Yinchuan 750004, China 2 Ningxia Engineering & Technology Research Center for Modernization of Hui Medicine, Yinchuan, 750004, China 3 Ningxia Collaborative Innovation Center for Hui Medicine, Yinchuan 750004, China 4 Key Laboratory of Hui Medicine Modernization, Ministry of Education, Yinchuan 750004, China 5 Ningxia Medicine Research Institute, Yinchuan 750004, China E-mail address: [email protected] (B. Changcai). Introduction: To provide a basis for further research and utilization of Radix Platycodi, the chemical constituents, biological activity, and quality control were reviewed. Methods: By literature reviewing and compilation of relevant data, literature on its chemical composition, biological activity and quality control studies were collected and analyzed. Results: The present chemical study of Platycodon grandiflorum were more focused on the composition of roots and less on other parts; its pharmacological activity mainly confined to the pharmacological activity of its water extract or total saponins, and reports on pharmacological activity of compounds which separated from P. grandiflorum were less. Conclusions: Future research on Radix Platycodi should focus in-depth on the chemical composition, and multiaspect biological activity and develop practical quality control indicators, which will provide scientific reference for its further study and comprehensive ultilization. http://dx.doi.org/10.1016/j.eujim.2014.09.088 New triterpenic acids and alkaloids from Uncaria rhynchophylla and their CID studies by use of an LTQ-Orbitrap Velos Pro mass spectrometer Wen-zhi Yang, Yi-bei Zhang, Wan-ying Wu, Min Yang, De-an Guo ∗ National Engineering Laboratory for TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Road, Shanghai 201203, PR China E-mail address: [email protected] (D.-a. Guo). Introduction: The genus Uncaria (Rubiaceae) is a crucial member of TCM family that has benefited human health
since ancient times. Alkaloids and triterpenes are two important groups of bioactive ingredients responsible for the therapeutic effects of Uncaria-derived traditional medicines. In the Chinese Pharmacopoeia (2010 edition), the stems and hooks of Uncaria rhynchophylla, U. macrophylla, U. hirsute, U. sinensis, and U. sessilifructus are collected as Uncariae Ramulus Cum Uncis (Gou-Teng) and indicated to treat dizziness, hyperpyrexia, and convulsion, etc. Notably, U. rhynchophylla and U. tomentosa are also considered as the promising agents to remedy Alzheimer’s disease and Parkinson disease, respectively. Previous phytochemical studies on U. rhynchophylla have resulted in the isolation of indole alkaloids, triterpenic acids, and phenolic compounds. Methods: With the view of exploring new bioactive chemicals in U. rhynchophylla, we conducted a systematic phytochemical study on the ethanol extracts of U. rhynchophylla. Results: Finally, 26 compounds, involving five new triterpenic acids, two new alkaloids, as well as 19 known compounds, were obtained. Of these, three triterpenic acids exhibited weak inhibitory effects on LPS-induced NO production in RAW264.7 cells. The CID-ESI-MSn behaviors of these pure compounds were comparatively studied by utility of an LTQ-Orbitrap Velos Pro mass spectrometer. 19-OH substituted oleanane triterpenic acids were prone to eliminate CH2 O3 (62 Da), whereas those ursane-type encompassing 19-OH were featured by preferred cleavage of H2 O (18 Da) while performing the negative CIDMS/MS fragmentation. In the case of alkaloids analyzed in the positive mode, diagnostic ions and characteristic cleavages were found that could assist in differentiating tetracyclic monotriterpene indole, tetracyclic monotriterpene oxidation indole, and tetracyclic monotriterpene indole N-oxide. Conclusions: A qualitative analysis strategy for characterizing the chemicals in Uncaria species is proposed, based on which 123 compounds in total were identified or tentatively characterized from four self-collected Uncaria plants. http://dx.doi.org/10.1016/j.eujim.2014.09.089 Antitussive and expectorant constituents of Reineckiacarnea (Andr.) Kunth Jun Yin ∗ , Yichun Wang, Ting Huang, Zhihui Liu, Na Han School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China E-mail address: [email protected] (J. Yin). Introduction: Reineckiacarnea (Andr.) Kunth, a kind of traditional ethnic drug, mainly is distributed in Guizhou and Yunnan provinces of southern China, whose aerial part is used for preventing cough, eliminating phlegm, rheumatism disease treatment and hepatitis. We aim to illustrate the active constituents of the aqueous extract of aerial part of Reineckiacarnea by bioassay guided isolation. Methods: The aqueous extract of Reineckiacarnea Herba was subjected to chromatographic separation on Diaion-101