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Researches on metabolic correlations and bio-economic impact in sheep populations affected by gestation toxemia
Abstracts / Journal of Biotechnology 185S (2014) S37–S125
in a logistic regression done by MINITAB 17. Results: Three models were found. The first model includes Mp (p = 0.014, OR = 4.9), Mo (p = 0.000, OR = 0.34), Bw (p = 0.014, OR = 5.79), Dr (p = 0.045, OR = 0.6). The second had Mp (p = 0.023, OR = 3.09), Dr (p = 0.036, OR = 0.69), LM (p = 0.033, OR = 0.73). The third model includes Lm (p = 0.077, OR = 0.80), Mp (p = 0.020, OR = 3.21) and Lc (p = 0.006, OR = 0.27). The log-likelihood ratio, the goodness of fit, and the measures of association test indicate that the model has a good predictive ability. Conclusion: Some variables can be considered indicators, but others are real risk factors in dairy farm. The succession of indicators or risk factors can be involved in milking technopathy of CM. http://dx.doi.org/10.1016/j.jbiotec.2014.07.170 Study of kidney of rats in effect of gamma radiation in the experiment
ages between 2 and 4 years old, presenting specific symptoms for gestational toxemia. Blood samples were taken before and after therapy. The clinical state was corroborated with the lab results. Seven metabolic parameters were screened (glycaemia, total protein, insulin, ketone bodies, cholesterol, calcium and magnesium). The results proved that only the insulin had decreased significantly (normal 0.44 ng/l) in all samples taken before the experiment, with tendency towards normal values after therapy. With the ketone bodies (normal 4 mg/dl), all values were significantly increased, 10–15 times higher, both before and after therapy. Gestational toxemia is not a common condition in sheep, however, when present, it can lead to significant economic losses. In the case at hand, the bio-economic impact was negative, the female and both fetuses died. http://dx.doi.org/10.1016/j.jbiotec.2014.07.172 Increased risk of disease in offspring of dogs with so-called “Merle” hair color
Makpal Baikazakova 1,∗ , Zura Yessimsiitova 2 , Zhannat Bazarbayeva 2 , Bekzat Tynybekov 2 , Assel Childibayeva 2 , Chinargul Aldaccugurova 2 , Bakyt Baigutdinova 1
Evelina Turnova 1,∗ , Zuzana Firdova 1 , Marcela Bielikova 2 , Andrej Dudas 2
1
1
Kazakh National Medical University named after S.D. Asfendiyarov, Almaty, Kazakhstan 2 Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: akmaral [email protected] (M. Baikazakova).
A special interest and value as a preventive and improving purpose are specialized products with polyfunctional destination, combining a number of medical and biological properties. The result of continued influence of adverse factors on the human body there is an infringement adaptation and self-regulation mechanisms of the body. At this stage, there are initial manifestations of metabolic disorders; there is an accumulation of toxic metabolic products in the body. Therefore, morphological study in rats with renal action of gamma – of irradiation using the immunnomodelirueschego specialized product was conducted. Experimental study on 30 adult rats experienced – male Wistar three months of age with an initial body weight of 280–300 g was conducted to identify adaptation opportunities rats receiving gamma irradiation at a dose of 6 Gy using a specialized imunnomodeling product. As a result of the complex macro- and microscopic studies on the background of a single total X-ray irradiation partly weakness, malaise, edema focal kidney epithelial cells were observed in rats. In rats treated for a month by immunomodeling specialized product, a decline of degenerative processes in the studied organs in the X-ray exposure, improve the body, increase the resistance of animals to the action of X-ray irradiation were observed. http://dx.doi.org/10.1016/j.jbiotec.2014.07.171 Researches on metabolic correlations and bio-economic impact in sheep populations affected by gestation toxemia Ionita Lucian ∗ , Danacu Valerica, Ionita Carmen, Popescu Liliana, Trambitas Bogdan, Vitalaru Bogdan Alexandru Faculty of Veterinary Medicine Bucharest, Romania E-mail address: [email protected] (I. Lucian). Gestational toxemia is a dysmetabolic syndrome, usually glucidic, involving disturbances of the lipid and protein metabolism. Researches were conducted on blood samples from 100 sheep, with
S51
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovak Republic 2 Slovgen s.r.o., Dúbravská cesta 21, 841 04 Bratislava, Slovak Republic E-mail address: [email protected] (E. Turnova). Irregular patches of diluted pigment that mixes with normal eumelanine characterize the so-called Merle coat color pattern. This discoloration occurs in the Australian Shepherd, Shetland Sheepdog, Collie and Dachshund breeds. Gene PMEL17 (Pre-melanosomal protein17) is associated with the merle phenotype in mice and dogs. PMEL17 gene encodes a membrane glycoprotein specific for melanocytes. The product of this gene produces melanocytes within the fibrous structure, which serve as a substrate for the precipitation and deposition of melanin. Merle retrotransposon SINE insertion has 253 bp and is located at the 10-dog chromosome. Dilution of pigments is generally caused by structural abnormalities in Pmel17, leading to disturbances in the formation of eumelanine. Increase risk of hearing abnormalities, degenerative eye diseases such as high in ocular pressure and microphthalmus, skeletal abnormalities, cardiovascular and reproductive system disorders and high mortality also occur for merle individuals. The genomic DNA was obtained from breeders and then the DNA was isolated. Region mutation was amplified by PCR followed by separation of the products by means of a horizontal agarose gel electrophoresis. This study is important for breeders, for compiling breeding pairs. Identified carriers of the cryptic merle mutation will be subjected to further analysis of PMEL17 gene. http://dx.doi.org/10.1016/j.jbiotec.2014.07.173
in a logistic regression done by MINITAB 17. Results: Three models were found. The first model includes Mp (p = 0.014, OR = 4.9), Mo (p = 0.000, OR = 0.34), Bw (p = 0.014, OR = 5.79), Dr (p = 0.045, OR = 0.6). The second had Mp (p = 0.023, OR = 3.09), Dr (p = 0.036, OR = 0.69), LM (p = 0.033, OR = 0.73). The third model includes Lm (p = 0.077, OR = 0.80), Mp (p = 0.020, OR = 3.21) and Lc (p = 0.006, OR = 0.27). The log-likelihood ratio, the goodness of fit, and the measures of association test indicate that the model has a good predictive ability. Conclusion: Some variables can be considered indicators, but others are real risk factors in dairy farm. The succession of indicators or risk factors can be involved in milking technopathy of CM. http://dx.doi.org/10.1016/j.jbiotec.2014.07.170 Study of kidney of rats in effect of gamma radiation in the experiment
ages between 2 and 4 years old, presenting specific symptoms for gestational toxemia. Blood samples were taken before and after therapy. The clinical state was corroborated with the lab results. Seven metabolic parameters were screened (glycaemia, total protein, insulin, ketone bodies, cholesterol, calcium and magnesium). The results proved that only the insulin had decreased significantly (normal 0.44 ng/l) in all samples taken before the experiment, with tendency towards normal values after therapy. With the ketone bodies (normal 4 mg/dl), all values were significantly increased, 10–15 times higher, both before and after therapy. Gestational toxemia is not a common condition in sheep, however, when present, it can lead to significant economic losses. In the case at hand, the bio-economic impact was negative, the female and both fetuses died. http://dx.doi.org/10.1016/j.jbiotec.2014.07.172 Increased risk of disease in offspring of dogs with so-called “Merle” hair color
Makpal Baikazakova 1,∗ , Zura Yessimsiitova 2 , Zhannat Bazarbayeva 2 , Bekzat Tynybekov 2 , Assel Childibayeva 2 , Chinargul Aldaccugurova 2 , Bakyt Baigutdinova 1
Evelina Turnova 1,∗ , Zuzana Firdova 1 , Marcela Bielikova 2 , Andrej Dudas 2
1
1
Kazakh National Medical University named after S.D. Asfendiyarov, Almaty, Kazakhstan 2 Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan E-mail address: akmaral [email protected] (M. Baikazakova).
A special interest and value as a preventive and improving purpose are specialized products with polyfunctional destination, combining a number of medical and biological properties. The result of continued influence of adverse factors on the human body there is an infringement adaptation and self-regulation mechanisms of the body. At this stage, there are initial manifestations of metabolic disorders; there is an accumulation of toxic metabolic products in the body. Therefore, morphological study in rats with renal action of gamma – of irradiation using the immunnomodelirueschego specialized product was conducted. Experimental study on 30 adult rats experienced – male Wistar three months of age with an initial body weight of 280–300 g was conducted to identify adaptation opportunities rats receiving gamma irradiation at a dose of 6 Gy using a specialized imunnomodeling product. As a result of the complex macro- and microscopic studies on the background of a single total X-ray irradiation partly weakness, malaise, edema focal kidney epithelial cells were observed in rats. In rats treated for a month by immunomodeling specialized product, a decline of degenerative processes in the studied organs in the X-ray exposure, improve the body, increase the resistance of animals to the action of X-ray irradiation were observed. http://dx.doi.org/10.1016/j.jbiotec.2014.07.171 Researches on metabolic correlations and bio-economic impact in sheep populations affected by gestation toxemia Ionita Lucian ∗ , Danacu Valerica, Ionita Carmen, Popescu Liliana, Trambitas Bogdan, Vitalaru Bogdan Alexandru Faculty of Veterinary Medicine Bucharest, Romania E-mail address: [email protected] (I. Lucian). Gestational toxemia is a dysmetabolic syndrome, usually glucidic, involving disturbances of the lipid and protein metabolism. Researches were conducted on blood samples from 100 sheep, with
S51
Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Mlynská dolina, 842 15 Bratislava, Slovak Republic 2 Slovgen s.r.o., Dúbravská cesta 21, 841 04 Bratislava, Slovak Republic E-mail address: [email protected] (E. Turnova). Irregular patches of diluted pigment that mixes with normal eumelanine characterize the so-called Merle coat color pattern. This discoloration occurs in the Australian Shepherd, Shetland Sheepdog, Collie and Dachshund breeds. Gene PMEL17 (Pre-melanosomal protein17) is associated with the merle phenotype in mice and dogs. PMEL17 gene encodes a membrane glycoprotein specific for melanocytes. The product of this gene produces melanocytes within the fibrous structure, which serve as a substrate for the precipitation and deposition of melanin. Merle retrotransposon SINE insertion has 253 bp and is located at the 10-dog chromosome. Dilution of pigments is generally caused by structural abnormalities in Pmel17, leading to disturbances in the formation of eumelanine. Increase risk of hearing abnormalities, degenerative eye diseases such as high in ocular pressure and microphthalmus, skeletal abnormalities, cardiovascular and reproductive system disorders and high mortality also occur for merle individuals. The genomic DNA was obtained from breeders and then the DNA was isolated. Region mutation was amplified by PCR followed by separation of the products by means of a horizontal agarose gel electrophoresis. This study is important for breeders, for compiling breeding pairs. Identified carriers of the cryptic merle mutation will be subjected to further analysis of PMEL17 gene. http://dx.doi.org/10.1016/j.jbiotec.2014.07.173