Retinol serum levels in ulcerative colitis: Correlation with disease activity

S82

Abstracts

Keywords: Sour cherry, Total antioxidant, Testosterone doi:10.1016/j.clinbiochem.2011.08.185

E Poster – [A-10-862-1] Effect of selenium supplementation on sFlt-1 level in pregnant women in high risk of pre-eclampsia Moosavy Mina, Tavallaee Shima, Ghayour Mobarhan Majid Bu-Ali Research Institute Ferdossi Square, Bu-Ali Square Mashhad, Iran E-mail addresses: [email protected] (M. Mina), [email protected] (T. Shima), [email protected] (G.M. Majid) Introduction: Preeclampsia is pregnancy-specific, and is a leading cause of prenatal and maternal morbidity and mortality. Oxidative stress associated with preeclampsia may be consequence of reduced antioxidant defense pathways specifically involving glutathione peroxidases, perhaps linked to reduce selenium availability. The sFlt-1, which contribute to endothelial dysfunction, is an alternative splice variant of VEGF-1 receptor able to act as an antagonist for both VEGF and PlGF. Material and method: This study examined the effect of selenium supplementation on sFlt-1 serum level in pregnant women in high risk of pre-eclampsia. In this pilot study, high risk pregnant women at the first trimester were selected randomly and were divided into two groups. Selenium group intake 100 μg/day selenium for 6 months as a case group and another group takes 100 μg/day placebo for 6 months. At the term, the blood samples were collected and the levels of sFlt-1 were measured. Results: Serum selenium levels were increased in the case group (p<0.05) from first to third trimester, but remained unchanged in the control group (p> 0.05). The results showed the sFlt-1 levels before and after use of supplementation had significantly differ in case and control groups. However, the changes between two groups did not reach statistical difference (p > 0.05). Conclusion: Selenium intake in the second and third trimester of pregnancy did not influence the sFlt-1 level in serum. This may be related to short period of using selenium intake or small sample size of this study. Keywords: Pre-eclampsia, Selenium, sFlt-1 doi:10.1016/j.clinbiochem.2011.08.1080

E Poster – [A-10-891-1] Red blood cell's (RBC's) folate concentration and the risk of colorectal adenoma (CRA) Bahmanpour Salmeh, Mazloom Zohreh, Tabei Seyyed Mohammad Bagher, Tabatabaee Hamidreza, Alizadeh Naeini Mahvash E-mail adrresses: [email protected], [email protected] (B. Salmeh), [email protected] (M. Zohreh), [email protected] (T.S.M. Bagher), [email protected] (T. Hamidreza) Background: Epidemiological, experimental studies, and clinical, mechanistic evidences suggested that higher plasma concentration of folate is associated with lower risk of colorectal carcinogenesis. However, only few studies assessed the role of red blood cell's folate concentration with colorectal adenoma. Objectives: We conducted a case–control study to determine the correlation between RBC's folate concentration and adenomatous polyps, the intermediate marker for colorectal cancer. Methods: This study included 177 cases with an adenomatous polyp (which are determined by pathologic data) in their colon by full colonoscopy

examination and 366 controls with no polyp at their full colonoscopy examination. Fasting blood samples were assayed by RIA Kit [Simul TRAC-Germany] to measured RBC's folate concentration. Other-related risk factors questionnaire were asked from all our study participants. Unconditional logistic regression was used to assess the odds ratio. Results: Data from 540 subjects were analyzed and the result showed that subjects with the RBC's folate levels of 140 ng/ml or more, have lower colorectal polyps compared to those with lower RBC folate level, the odds ratio was 0.96 [95% Confidence Interval (CI) = 0.712– 1.465]; P-value (0.945). Conclusion: RBC's folate concentration has an effect on the development of colorectal polyps. The result of this study showed a protective effect of RBC's folate concentration against the development of colorectal polyps. A lower risk of CRA was seen in subjects with higher RBC's folate concentration. Keywords: Colorectal adenoma, CRA, RBC's folate, RBC, Folate doi:10.1016/j.clinbiochem.2011.08.1081

E Poster – [A-10-987-1] Effect of vitamin A supplementation on inflammatory markers in patients with ulcerative colitis Abedi Manesh Nasim, Ostadrahimi Alireza, Soomi Mohammad Hosein, Alipour Beitollah Tabriz University of Medical Sciences, Tabriz, Iran E-mail address: [email protected] (A.M. Nasim) Introduction: Ulcerative colitis (UC) is a chronic inflammatory disease of the rectal and colonic mucosa and seems to result from a complex series of interactions between susceptibility genes, the environment and the immune system. There is a paucity of data on the positive effect of vitamin A on intestinal mocusal immunity. We aimed to evaluate the effect of vitamin A supplementation on proinflammatory cytokine interleukin 12 (IL12) and acute phase protein , high sensitive-C-reactive protein(hs-CRP) and anti-inflammatory cytokines, IL4 and IL10 in ulcerative colitis. Methods: Immunological assessment was done in 43 out patients with ulcerative colitis participating in a before and after interventional survey in Sheikh al rais, a public clinic of Tabriz. All participants were at clinical remission stages. The severity of disease activity was assessed by Truelove–Witt Index. Vitamin A supplement was injected twice (50,000 IU) with two weeks interval. Study period was considered 45 days. Serum interleukins and hs-CRP levels were measured by ELISA and turbidimetric methods, respectively. Results: Vitamin A supplementation had significant effect on decrease of IL12 (P= 0.003) and clinical symptom severity (P =0.001). IL4 increased considerably (P= 0.001) while hs-CRP and IL10 changes were not significant. Conclusion: Vitamin A supplementation could suppress serum proinflammatory cytokines and be beneficial in reduction of clinical symptom severity of disease in ulcerative colitis in remission and should be explored in a larger study. Keywords: Ulcerative colitis, Cytokine, Inflammation doi:10.1016/j.clinbiochem.2011.08.1082

Poster – [A-10-987-2] Retinol serum levels in ulcerative colitis: Correlation with disease activity Abedi Manesh Nasim, Ostadrahimi Alireza, Soomi Mohammad Hosein, Alipour Beitollah

Abstracts

Tabriz University of Medical Sciences, Tabriz, Iran E-mail address: [email protected] (A.M. Nasim) Introduction: Hypovitaminosis and fat-soluble vitamin deficiency have been reported in adults with ulcerative colitis(UC). A prospective pilot study was undertaken to determine the prevalence of low serum levels of vitamin A in adults with UC. Methods: Clinical information and serum for vitamin levels were gathered prospectively from 46 patients with ulcerative colitis. Disease activity and disease location were determined for UC patients by Truelove–Witt Index. Serum retinol levels were determined by high-performance liquid chromatography. Results: The prevalence of hypovitaminosis A (defined as serum vitamin A <20 μg/dl) was 34% in the ulcerative colitis population studied. Serum retinol levels correlated negatively with disease activity(P<0.05). Conclusions: Adults with active ulcerative colitis frequently have low serum levels of vitamin A. The severity of disease activity is a useful predictor of risk for hypovitaminosis. Keywords: Ulcerative colitis, Retinol

doi:10.1016/j.clinbiochem.2011.08.1083

E Poster – [A-10-1026-1] Flow cytometric analysis of the effect of dimethylsulfone on cell cycle arrest in human cancer cell lines Jafari Naser, Bohlooli Shahab Ardabil University of Medical Sciences, Ardabil, Iran E-mail address: [email protected] (J. Naser) Introduction: Dimethylsulfone (MSM) is a dietary supplement to support healthy body. It has been suggested that MSM has a chemopreventive mechanism that affects the interaction of tumor cells with the host immune response. Considering preventive effects of this substance on tumor onset and non-toxic to healthy body we investigated in vitro effects of dimethylsulfone on cell cycle distribution in cancer cell lines. Materials and methods: Cells were seeded in 25 cm2 flasks at a density of 1 × 106 cells/flask. Cells were treated with MSM for 24 h at a range of concentration. After treatment, the DNA content and cellcycle distribution were determined by flow cytometry. Briefly, cells were trypsinized and harvested, then cells fixed with 70% ice ethanol. Fixed cells were centrifuged and washed with cold PBS, and then stained with DAPI 10 μg/ml. The stained cells were then transferred to flow tubes by passing through a nylon mesh with a pore size of 30 μm. Results: The percentage of cells in G1, S and G2/M phase was calculated using Partec FloMax software. We observed a dosedependent effect of MSM on the cell cycle. After 24 h of MSM treatment at different concentrations, cells in the G2/M population increased compared to controls. The effect of MSM on cancer cells appears to be dose-dependent. The higher the dosage, the greater the G2/M population increases. Conclusion: The results showed that MSM is able to induce a G2/ M cell cycle arrest in gastrointestinal cancer cell lines. Keywords: Dimethylsulfone, Dietary supplement, Cell cycle, Cancer cell lines

doi:10.1016/j.clinbiochem.2011.08.1084

S83

E Poster – [A-10-1152-1] Effect of glycemic control on prooxidant–antioxidant balance in type 2 diabetic patients and its comparison with healthy subjects Heidari Hossein, Rajai Zahra, Khozeime Fahime, Anvari Milad, Hadizadeh yazdi Niloofar, Norouzy Abdolreza, Soukhtanloo Mohammad, Bonakdaran Shokoufeh, Nejatshokouhi Amireh MUMS, Mashhad, Iran E-mail addresses: [email protected] (H. Hossein), [email protected] (R. Zahra), [email protected] (K. Fahime), [email protected] (A. Milad), [email protected] (H. Niloofar), [email protected] (N. Abdolreza), [email protected] (S. Mohammad), [email protected] (B. Shokoufeh), [email protected] (N. Amireh) Introduction: Diabetes is associated with an increased risk of vasculopathy. Hyperglycemia is a major factor in increasing free radicals and oxidative stress. In some studies, it has been hypothesized that vasculopathy may not be affected by glycemic control. In this study, we compared the PAB in well-controlled and poor controlled type 2 diabetic patients and compared it with a sex and age matched healthy control group. Method: 150 patients including 50 well controlled type 2 diabetic patients (HbA1c 6.9 ± 0.7%, age 50 ± 8), 50 poor controlled type 2 diabetic patients (HbA1c11.7 ± 1.7%, age 49 ± 11) and 50 age and sex matched healthy subjects (age 46 ± 8) were selected for this study. PAB was measured with a novel assay which is based on 3,3′,5,5′tetramethylbenzidine and its cation. Result: In poor controlled diabetic patients mean PAB value was significantly higher compared to well controlled diabetic and healthy subjects (90 ± 33, 57 ± 36 and 52 ± 9 HK respectively) (ANOVA, P<0.0001). Conclusion: We concluded the PAB is higher in poor controlled type 2 diabetic patients, showing an elevated oxidative stress. This may show the importance of glycemic control in prevention of cardiovascular events in diabetic patients. Keywords: Type 2 diabetes, Glycemic control, HbA1c, Prooxidant– antioxidant balance, Cardiovascular disease

doi:10.1016/j.clinbiochem.2011.08.1085

E Poster – [A-10-1153-1] Comparison of serum oxidized LDL level in healthy and well glycemic controlled type 2 diabetic subjects Khozeime Fahime, Rajai Zahra, Anvari Milad, Heidari Hossein, Hadizadeh Yazdi Niloofar, Norouzy Abdolreza, Soukhtanloo Mohammad, Bonakdaran Shokoufeh, Nejatshokouhi Amireh Mashhad University of Medical Sciences, Mashhad, Iran E-mail addresses: [email protected] (K. Fahime), [email protected] (R. Zahra), [email protected] (A. Milad), [email protected] (H. Hossein), [email protected] (H.Y. Niloofar), [email protected] (N. Abdolreza), [email protected] (S. Mohammad), [email protected] (B. Shokoufeh), [email protected] (N. Amireh) Introduction: Oxidized LDL is a form of low-density lipoprotein (LDL) which has been attacked by oxygen free radicals when it enters to the endothelium. Oxidized LDL (Ox-LDL) is known to promote atherogenesis which is the major cause of cardiovascular disease. This study was conducted to compare the serum oxidized low density