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RETROSPECTIVE ANALYSIS OF 598 ORAL CANCER CASES IN DENTISTRY SCHOOL
ABSTRACTS
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FERNANDEZ BARRIALES, MARIA LUISA GAINZACIRAUQUI and, JOSE MANUEL AGUIRRE-URIZAR Objective: To validate oral exfoliative cytology in the analysis of the microRNA expression profile in oral lichenoid disease (OLD). Study Design: The expression of 13 microRNAs was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytologic samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using reverse transcription-quantitative polymerase chain reaction and TaqMan microRNA assays. Results: The aberrant expression was validated for 2 microRNAs (miR-146 a-5 P and miR-7-1-3 P) of those previously recognized in the biopsy study. Conclusion: This is the first time that oral exfoliative cytology is validated in a microRNA study in OLD. The microRNAs validated in this study are associated with both inflammatory and carcinogenic phenomena that are involved in the etiopathogenesis of this potentially malignant oral disorder.
ORAL CARCINOGENESIS BIOMARKERS IN ORAL CHRONIC MECHANICAL IRRITATION-ASSOCIATED LESIONS. GERARDO GILLIGAN, EDUARDO PIEMONTE, RENE PANICO, CECILIA DI TADA and, MABEL BRUNOTTO Previous works have shown clinical data suggesting that chronic mechanical irritation (CMI) could be a risk factor for oral cancer (OC). However, there are no known patterns of biomarkers in CMI indicative of cellular malignancy. Objective: The aim of this study was to identify a biomarker pattern of differentiation (Ck19) and cell proliferation (Ki67) in CMI-associated lesions similar to those observed in OC. Study Design: A cross-sectional study with adult patients (n = 61) of both sexes with lesions associated with CMI and with oral cancer were included. CK19 and Ki67 immunohistochemistry were carried out in specimens of both types of lesions, and the patterns were described according to previous publications. The present study was approved by Ethics Committee of the College of Dentistry, Universidad Nacional de Cordoba. Results: Ki67 overexpression and a CK19 focal pattern with strong intensity in basal and parabasal cells were observed in CMI lesions, similar to that observed in OC. Conclusion: There is no scientific literature on biomarker patterns of oral CMI. Therefore, the described pattern, particularly Ck19, is novel and could be indicative of the relation between CMI and oral carcinogenesis.
RETROSPECTIVE ANALYSIS OF 598 ORAL CANCER CASES IN DENTISTRY SCHOOL. MARIA FERNANDA LOPES FONSECA, ^ GO, SARAH CAMPOS DE SALES, CAIQUE ROCHA PE DANIEL MENEZES SANTOS and, VAGNER RODRIGUES SANTOS The aim of this study is to present occurrence demographic data of patients diagnosed as having squamous cell carcinoma in dentistry school. Study Design: A total of 598 diagnosed clinical charts were analyzed. Histopathologic reports of all patients with diagnosed oral cancer from 1956 to 2010 were analyzed following inclusion
OOOO January 2020 and exclusion criteria. Information about sex, age, skin color, disease time report, and injury site was collected, and statistical analysis was done in the software Microsoft Excel 2013. Results: The sample consisted of 24.75% women and 74.75% men. Regarding skin color, 41.64% were white, 32.94% were African-white, and 17.89% were of African descent. The majority of patients were in the sixth (28.93%) and seventh (20.74%) decades of life. For the injury location, the main sites were the tongue (165), floor of mouth (123), and alveolar mucosa (117) cases. The injury time ranged from less than 1 month to 11 years. Reported time of disease duration was up to 3 months. Oral cancer affected more white women in their 70th decade and more white men in their 60th decade of life. Conclusion: Epidemiologic studies are important to contribute to the database on the Minas Gerais State and Brazil cancer occurrence, which may help prevention strategies. Support: FAPEMIG.
SPATIAL DISTRIBUTION OF ORAL CANCER IN AN ONCOLOGY HOSPITAL IN CURITIBA/BRAZIL: A PILOT STUDY. ROBERTA TARGA STRAMANDINOLI-ZANICOTTI, NATALI LEIDENS, LARISSA BALBO ZAVAREZ, LAURINDO MOACIR SASSI, GYL HENRIQUE ALBRECHT RAMOS and, CASSIUS CARVALHO TORRES-PEREIRA Objective: The aim of this study was to evaluate the correlation between the prevalence of oral cancer cases with their geographic position. Study Design: This pilot, observational, retrospective, and descriptive research was developed in a south Brazilian city (Curitiba/Parana) for 1 year. The inclusion criteria were histologic diagnosis of squamous cell carcinoma affecting oral cavity, lips, and oropharynx. Data were collected from an oncology hospital electronic database and displayed in a spreadsheet containing information such as sex, ethnicity, age, level of education, and patient zip code. The mapping was performed with the support of an electronic map application, using the patients’ zip codes. Results: Of a total of 93 cases, 72 individuals were mapped; 60 men and 12 women with a mean age of 60 years old were distributed by 10 neighborhoods of Curitiba (n = 45) and by 9 cities of metropolitan area (n = 27). The majority of the sample consisted of white people (97%) with primary level education who were clustered around peripheral areas of the city. Conclusion: There was a tendency of marginalization in the spatial distribution of oral cancer cases in Curitiba, and the method was effective for the mapping, enabling, and encouraging new studies in epidemiology of oral cancer.
RISK FACTORS FOR ORAL CANCER IN YOUNG PEOPLE IN CORDOBA, ARGENTINA. LORENA MOINE, GERARDO GILLIGAN, EDUARDO PIEMONTE and, RENE PANICO In the last decades, an increase in the incidence rates of oral squamous cell carcinoma has been reported in patients younger than 45 years. Objective: To describe clinical and demographic characteristics and risk factors in patients younger than 45 years with squamous cell carcinoma of the oral mucosa. Study Design: A total of 12 patients younger than 45 years were included in a retrospective descriptive study from 2009 to 2017. Variables registered were age, sex, tumor location,
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FERNANDEZ BARRIALES, MARIA LUISA GAINZACIRAUQUI and, JOSE MANUEL AGUIRRE-URIZAR Objective: To validate oral exfoliative cytology in the analysis of the microRNA expression profile in oral lichenoid disease (OLD). Study Design: The expression of 13 microRNAs was analyzed in 26 cases, 16 diagnosed as OLD and 10 controls with no oral mucosal pathology. Cytologic samples from the oral mucosa obtained using an Orcellex toothbrush were analyzed using reverse transcription-quantitative polymerase chain reaction and TaqMan microRNA assays. Results: The aberrant expression was validated for 2 microRNAs (miR-146 a-5 P and miR-7-1-3 P) of those previously recognized in the biopsy study. Conclusion: This is the first time that oral exfoliative cytology is validated in a microRNA study in OLD. The microRNAs validated in this study are associated with both inflammatory and carcinogenic phenomena that are involved in the etiopathogenesis of this potentially malignant oral disorder.
ORAL CARCINOGENESIS BIOMARKERS IN ORAL CHRONIC MECHANICAL IRRITATION-ASSOCIATED LESIONS. GERARDO GILLIGAN, EDUARDO PIEMONTE, RENE PANICO, CECILIA DI TADA and, MABEL BRUNOTTO Previous works have shown clinical data suggesting that chronic mechanical irritation (CMI) could be a risk factor for oral cancer (OC). However, there are no known patterns of biomarkers in CMI indicative of cellular malignancy. Objective: The aim of this study was to identify a biomarker pattern of differentiation (Ck19) and cell proliferation (Ki67) in CMI-associated lesions similar to those observed in OC. Study Design: A cross-sectional study with adult patients (n = 61) of both sexes with lesions associated with CMI and with oral cancer were included. CK19 and Ki67 immunohistochemistry were carried out in specimens of both types of lesions, and the patterns were described according to previous publications. The present study was approved by Ethics Committee of the College of Dentistry, Universidad Nacional de Cordoba. Results: Ki67 overexpression and a CK19 focal pattern with strong intensity in basal and parabasal cells were observed in CMI lesions, similar to that observed in OC. Conclusion: There is no scientific literature on biomarker patterns of oral CMI. Therefore, the described pattern, particularly Ck19, is novel and could be indicative of the relation between CMI and oral carcinogenesis.
RETROSPECTIVE ANALYSIS OF 598 ORAL CANCER CASES IN DENTISTRY SCHOOL. MARIA FERNANDA LOPES FONSECA, ^ GO, SARAH CAMPOS DE SALES, CAIQUE ROCHA PE DANIEL MENEZES SANTOS and, VAGNER RODRIGUES SANTOS The aim of this study is to present occurrence demographic data of patients diagnosed as having squamous cell carcinoma in dentistry school. Study Design: A total of 598 diagnosed clinical charts were analyzed. Histopathologic reports of all patients with diagnosed oral cancer from 1956 to 2010 were analyzed following inclusion
OOOO January 2020 and exclusion criteria. Information about sex, age, skin color, disease time report, and injury site was collected, and statistical analysis was done in the software Microsoft Excel 2013. Results: The sample consisted of 24.75% women and 74.75% men. Regarding skin color, 41.64% were white, 32.94% were African-white, and 17.89% were of African descent. The majority of patients were in the sixth (28.93%) and seventh (20.74%) decades of life. For the injury location, the main sites were the tongue (165), floor of mouth (123), and alveolar mucosa (117) cases. The injury time ranged from less than 1 month to 11 years. Reported time of disease duration was up to 3 months. Oral cancer affected more white women in their 70th decade and more white men in their 60th decade of life. Conclusion: Epidemiologic studies are important to contribute to the database on the Minas Gerais State and Brazil cancer occurrence, which may help prevention strategies. Support: FAPEMIG.
SPATIAL DISTRIBUTION OF ORAL CANCER IN AN ONCOLOGY HOSPITAL IN CURITIBA/BRAZIL: A PILOT STUDY. ROBERTA TARGA STRAMANDINOLI-ZANICOTTI, NATALI LEIDENS, LARISSA BALBO ZAVAREZ, LAURINDO MOACIR SASSI, GYL HENRIQUE ALBRECHT RAMOS and, CASSIUS CARVALHO TORRES-PEREIRA Objective: The aim of this study was to evaluate the correlation between the prevalence of oral cancer cases with their geographic position. Study Design: This pilot, observational, retrospective, and descriptive research was developed in a south Brazilian city (Curitiba/Parana) for 1 year. The inclusion criteria were histologic diagnosis of squamous cell carcinoma affecting oral cavity, lips, and oropharynx. Data were collected from an oncology hospital electronic database and displayed in a spreadsheet containing information such as sex, ethnicity, age, level of education, and patient zip code. The mapping was performed with the support of an electronic map application, using the patients’ zip codes. Results: Of a total of 93 cases, 72 individuals were mapped; 60 men and 12 women with a mean age of 60 years old were distributed by 10 neighborhoods of Curitiba (n = 45) and by 9 cities of metropolitan area (n = 27). The majority of the sample consisted of white people (97%) with primary level education who were clustered around peripheral areas of the city. Conclusion: There was a tendency of marginalization in the spatial distribution of oral cancer cases in Curitiba, and the method was effective for the mapping, enabling, and encouraging new studies in epidemiology of oral cancer.
RISK FACTORS FOR ORAL CANCER IN YOUNG PEOPLE IN CORDOBA, ARGENTINA. LORENA MOINE, GERARDO GILLIGAN, EDUARDO PIEMONTE and, RENE PANICO In the last decades, an increase in the incidence rates of oral squamous cell carcinoma has been reported in patients younger than 45 years. Objective: To describe clinical and demographic characteristics and risk factors in patients younger than 45 years with squamous cell carcinoma of the oral mucosa. Study Design: A total of 12 patients younger than 45 years were included in a retrospective descriptive study from 2009 to 2017. Variables registered were age, sex, tumor location,