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Rhesus disease: intravascular fetal blood transfusion by cordocentesis
186 Y = 0.47 +0.08X, a variance of 0.889 and r = 0.92, P < 0.001. It was concluded that the computed flow parameter from laser Doppler spectroscopy varied linearly with CBF and offers the unique advantage of continuous measurement even during nonsteady state flow. Financial support was granted by the National fund for Research into Crippling Diseases: Action Research. 1 M.A. Heymann, B.D. Payne, J.I.E. Hoffman and A.M. Rudolph (1977): Prog. Cardiovasc. Dis., XX, 55-79.
Rhesus diserse: intravascular fetal blood transfusion by cordocentesis. Kypros Nicolaides, Peter Soothill and Katia Bilardo, Harris Birthright Trust, Department of Obstetrics, King’s College Hospital, London, U.K. A total of 63 intravascular fetal blood transfusions were given by cordocentesis in 15 pregnancies complicated by red cell iso-immunisation. Transfusions were commenced at 19-34 weeks gestation and were repeated several times (l-7) at 2-4 weekly intervals. Final transfusions were given at 31-36 weeks and the infants delivered at 31-39 weeks. One baby was stillborn and 14 are alive and well. The volumes and rates of transfusions and their effect on the fetal acid base status and blood flow velocity in the fetal aorta are presented.
Fluorimetry
Aberdeen.
in the study of infant gut flora. S.J. Rose, Department
of Child Health, University of
U.K.
There is still controversy as to the normal infant gut flora and whether breast fed infants have a different flora from artificially fed infants. This may be due to the inherent problems of quantitative stool culture which can introduce an error of a factor of lo4 or that the modifications of cows’milk have now minimised biochemical differences, compared to breast milk. Escherichiu coli is still a major cause of gastroenteritis, and is inhibited by the environment favourable to lactobacilli. reportedly in excess in breast fed infants. Thus. a reproducible biochemical analysis which correlated with bacterial populations would allow bacterial changes to be followed early in communities and also interlaboratory comparison would improve. Escherichin coli produces mainly @ glucurosidase whilst lactobacilli and bifido-bacteria produces mainly /3 galactosidase. The infant stool flora becomes established during the first week of life and is relatively stable thereafter. these changes may be reflected in enzyme activity. 130 samples were studied. 45 from breast fed infants. 85 from artificially fed infants over the first six weeks of life. Stools were cultured quantitatively for Eschen’chia co/i. lactobaciili and bifidobacteria. enzyme activity was determined fluorimetrically using methylumbelliferone derivative substrates. Correlation was found neither between individual culture and enzyme activity results nor between collated results. The possible reasons for this lack of correlation will be discussed.
Dynamic lung inflation during high frequency oscillation in respiratory distress syndrome. E.W. Hoskyns.
A.D. Milner and I.E. Hopkin. Nottingham, U.K.
Department
of Neonatal
Medicine
and Surgery,
City Hospital.
High frequency oscillation (HFO) has been used successfully in infants with R.D.S. Animal and human data indicate that this is associated with lung inflation due to alveolar (inadvertent) positive pressure. This pressure is not measurable at the airway opening and. if large. could affect cardiac output. This study was mounted to investigate this effect in neonates.
Rhesus diserse: intravascular fetal blood transfusion by cordocentesis. Kypros Nicolaides, Peter Soothill and Katia Bilardo, Harris Birthright Trust, Department of Obstetrics, King’s College Hospital, London, U.K. A total of 63 intravascular fetal blood transfusions were given by cordocentesis in 15 pregnancies complicated by red cell iso-immunisation. Transfusions were commenced at 19-34 weeks gestation and were repeated several times (l-7) at 2-4 weekly intervals. Final transfusions were given at 31-36 weeks and the infants delivered at 31-39 weeks. One baby was stillborn and 14 are alive and well. The volumes and rates of transfusions and their effect on the fetal acid base status and blood flow velocity in the fetal aorta are presented.
Fluorimetry
Aberdeen.
in the study of infant gut flora. S.J. Rose, Department
of Child Health, University of
U.K.
There is still controversy as to the normal infant gut flora and whether breast fed infants have a different flora from artificially fed infants. This may be due to the inherent problems of quantitative stool culture which can introduce an error of a factor of lo4 or that the modifications of cows’milk have now minimised biochemical differences, compared to breast milk. Escherichiu coli is still a major cause of gastroenteritis, and is inhibited by the environment favourable to lactobacilli. reportedly in excess in breast fed infants. Thus. a reproducible biochemical analysis which correlated with bacterial populations would allow bacterial changes to be followed early in communities and also interlaboratory comparison would improve. Escherichin coli produces mainly @ glucurosidase whilst lactobacilli and bifido-bacteria produces mainly /3 galactosidase. The infant stool flora becomes established during the first week of life and is relatively stable thereafter. these changes may be reflected in enzyme activity. 130 samples were studied. 45 from breast fed infants. 85 from artificially fed infants over the first six weeks of life. Stools were cultured quantitatively for Eschen’chia co/i. lactobaciili and bifidobacteria. enzyme activity was determined fluorimetrically using methylumbelliferone derivative substrates. Correlation was found neither between individual culture and enzyme activity results nor between collated results. The possible reasons for this lack of correlation will be discussed.
Dynamic lung inflation during high frequency oscillation in respiratory distress syndrome. E.W. Hoskyns.
A.D. Milner and I.E. Hopkin. Nottingham, U.K.
Department
of Neonatal
Medicine
and Surgery,
City Hospital.
High frequency oscillation (HFO) has been used successfully in infants with R.D.S. Animal and human data indicate that this is associated with lung inflation due to alveolar (inadvertent) positive pressure. This pressure is not measurable at the airway opening and. if large. could affect cardiac output. This study was mounted to investigate this effect in neonates.