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Role of nitric oxide in regulating cytokine expression and tissue injury in postischemic liver injury
microvesicularfatty changein zone one,ln(G2}tattychangewas more severe.Theiollammatory infiltrate was scanty and no fibrosis was seen in all groups.Oxidativestress was increased in(G2) as well in(G1) as compared with controls(table below).Biochemicalvariables are displayed in the table below: Conclusion:Oxidetivestress measured by lucigenin-amplified luminescencewas increasedin experimentalhepaticsteatosis,animportant step in the pathogenesis of NASH. Table(Mean±SD) Group
N
AST
ALT
Cholest
Tdglyc.
Lumines C.
Gt
6
108-+31,
40-+1
36_+1
88_+3*
G2
6
158-+6.
27-+8
55±1
146-+3.
G3
6
26-+2
34_+5
40-+7
45~7
1393_+79 O* 7191_+50 O* 513±170
study. In 1996 and 1997these patientsunderwentliver resectiondue to CCC.Tissue specimens were immediately taken after resection and processed. After staining DNA-cytometry was carried out on more than 100 single tumor cells and results were given as diploid, polyploid or aneuploid chromosomal set numbers and tumors respectively. Patientswere followed up for 1 to 4 years. DNA-ploiditywas comparedto establishedprognostic parametersin predicting the survival probability. RESULTS:Tumor staging assigned4% of patientsto the tumor stage pT1, 18% to stage pT2, 56% to stage pT3 and 22% to stage pT4. Tumor grading showed predominantly grade 2 (70%) and less frequent tumor grade 3 (26%) and grade 1 (4%). DNA-analysisclassified tumors either as diploid (22%), polyploid (15%) or aneuploid (63%). All patients which revealedaneuploidtumor cell lines at time of operation died within 3 years whereas most patients afflicted from a diploid tumor (83%) survived more than 4 years. DNA ploidity was better in predicting the survival of these patients than tumor stage, grade, stage of metastasisand completness of tumor resection. CONCLUSION:DNA ploidity was the best prognosis factor to predict survival probability of liver resected patieo1~suffering from COG. Patients with diploid CCC survived for a long time whereasaneuploidtumors indicateda poor prognosis with a rather short survival time. We conclude that DNA-ploidity is a valuable diagnostic tool for identifying subgroups of patients that may be at higher risk for tumor progression and might not benefit from liver resection.
*P
2783 2780
Clinical Utility Of Color Doppler Ultmcanography In Pediatric Liver TrausplantaUon From Living Donors Masazumi None Fujimoto, Shiga Medical Ctr for Adult Diseases, Moriyama-shi, Shiga Japan; Kazuhiko None Mizuta, Shiga Medical Ctr, Moriyama-shi, Shiga Japan; Fuminori None Moriyasu, Tokyo Medical Univ, Shinjuku-ku, Tokyo Japan; Tsutomu None Chiba, Koichi None Tanaka, Kyoto Univ Fac of Med, Kyoto-shi, Kyoto Japan
Oral Administration of Upopolysaccharides Synergically Enhances Liver injury Caused by Chronic Ethanol Feeding in Rats - A New Animal Model of Alcoholic Hepatitis Hironao Tamai, Yoshinori Horie, Shinzo Kato, Eiji Ohki, Hiromasa Ishii, Keio Univ Sch of Medicine, Tokyo Japan Background and aims: Endotoxin has been implicated in the pathogenesisand progression of alcoholic liver disease. However, it is still unclear how chronic ethanol feeding affects absorption of endotoxin from the intestine, and susceptibility of the liver to gut-derived endotoxin. The aim of this study was to determine the effect of chronic ethanol feeding on susceptibility of the liver to orally administeredendotoxin. Methods: Male Wistar rats weighing about 150g were pair fed with ethanol containing liquid diet or control diet for 5 weeks. In another set of animal feeding, 0,10 or 20 mg/kg of tipopolysaccharides(LPS) were added to the liquid diet for 7days beginning on Day 29~. On Day 3P, the animals were sacrificed for blood biochemistry and histological examination of the liver. Plasma endotoxin levels were also determined after 20mg/kg LPS administration using a gastric tube. In another set of experiments, we determined intestinal permeability using FD4 (fluorescein isothiocyanatelabeled dextran with an averagemolecular weight 4000 D). Results: With 10 mg/kg of LPS, serum ALT and ALP levels were significantly increased in ethanol fed rats, which were not noted in the controls. After 20 mg/kg of LPS administration, more substantial increases in serum ALT and ALP levels were observed in ethanol-fed rats compared to pair-fed rats. Neutrophil infiltration, ballooning and spotty necrosis were prominent in rats given LPS in ethanol containing diets. Plasmaendotoxin levels in chronic ethanol-fed rats were higher than those in pair-fed rats. Furthermore, the intestinal permeability to FD4 was increased by chronic ethanol administration. Conclusion:Theseresults suggestthat chronic ethanolfeeding increasesthe intestinal permeabilityand absorption of endotoxin, resulting in an enhancement of the susceptibility of the liver to orally administeredendotoxin.This model servesa subclinical experimental model for the study of alcoholic liver disease. 2781 Change Of Hepatic EnzymesAfter Acute Alcohol intake in Non-Soffish Volunteers Youming Li, Min Yue, Chaohui Yu, Weixing Chen, Gengyun Xu, 1st Affiliated Hosp, Hangzhou People'S Rep Of China Objectives:Changesin variable hepatic functional marks after acute alcohol intake in nonsottish volunteers are discussed to clarify the influence of acute alcohol intake on liver and the importance of variable marks under different blood alcohol level and time points. Methods:Affer 15 volunteers have drunken 182mL white wine which contains 80g alcohol in half an hour, blood samples are taken at 8 intervals while 0.5,1,1.5,2,2.5,3,6,24hours from the very beginningto detect ALT,AST,NAPand ~t-GT.Meanwhilethe blood alcohol level, heart rate, blood pressure and mental state are inspected. Results:The ratio of alt/ast increases after single alcohol intake while the blood alcohol level is higher than 1.2g/L with P value less than 0.05. NAP and ~/-GT also increase while the blood level is less than O.4g/L with p value less than 0.01 and 0.0001. No marks make significant changes on different time points. Conclusions: We may predict that acute alcoholism may induce the changes of liver enzymic system and this supports the multiple organic injury of alchhol.
Using color Doppler US, we examined hepatic blood flow to assess vascular anastomosis intraoperatively and for postoperatve monitoring of graft circulation in 122 pediatric liver transplants from living donors. Our examinationsfocused on (1) peak systolic velocity (Vp) and pulsatility index (PI) of the hepaticartery, (2) blood flow in the portal vein and spontaneous portosystemic collateral pathways, and (3) flow velocity and waveforms in the hepatic vein. (1) Six cases with hepatic artery thrombosis were detected by Doppler US. In 2 cases with dampened waveforms at intraoperative flowmetry (Vp-<23 cm/s and PI-
2782 DNA-ploidlty Predicts Clinical OutcomeAfter Liver Resection Due to Cholangiocarcinoma Hassan Abou-Rebyeh, Dept Internal Medicine, Hepatologyand Gastroenterology,Berlin Germany; Hussain AI-Abadi, Sven Jonas, Inga Rotter, Dept Gen, Visceral and Transplantation Surg, Berlin Germany; Wilfded Voltzke-Schlieker,Andreas Adler, Rainer E. Hintze, Bertram Wiedenmann, Dept Internal Medicine, Hepatologyand Gastroenterology, Berlin Germany; Peter Neuhaus, Dept Gun, Visceral and Transplantation Surg, Berlin Germany Background: Many patients afflicted from cholangiocarcinoma (CCC) undergo partial liver resection. A variety of prognostic factors are used to predict their clinical outcome. So far, tumor stage, grade, stage of metastasis and completenessof tumor resection are used as prognostic factors to predict the outcome of CCC-patients after liver resection. But their predictive value seems to be limited. We investigated DNA-ploidity in terms of chromosomal set nnmber ol CCCtumor cell lines at time of operationwhich might be an alternativeparameter for prediction of survival probability. METHODS:We included 27 patients into a prospective
A-547
N
AST
ALT
Cholest
Tdglyc.
Lumines C.
Gt
6
108-+31,
40-+1
36_+1
88_+3*
G2
6
158-+6.
27-+8
55±1
146-+3.
G3
6
26-+2
34_+5
40-+7
45~7
1393_+79 O* 7191_+50 O* 513±170
study. In 1996 and 1997these patientsunderwentliver resectiondue to CCC.Tissue specimens were immediately taken after resection and processed. After staining DNA-cytometry was carried out on more than 100 single tumor cells and results were given as diploid, polyploid or aneuploid chromosomal set numbers and tumors respectively. Patientswere followed up for 1 to 4 years. DNA-ploiditywas comparedto establishedprognostic parametersin predicting the survival probability. RESULTS:Tumor staging assigned4% of patientsto the tumor stage pT1, 18% to stage pT2, 56% to stage pT3 and 22% to stage pT4. Tumor grading showed predominantly grade 2 (70%) and less frequent tumor grade 3 (26%) and grade 1 (4%). DNA-analysisclassified tumors either as diploid (22%), polyploid (15%) or aneuploid (63%). All patients which revealedaneuploidtumor cell lines at time of operation died within 3 years whereas most patients afflicted from a diploid tumor (83%) survived more than 4 years. DNA ploidity was better in predicting the survival of these patients than tumor stage, grade, stage of metastasisand completness of tumor resection. CONCLUSION:DNA ploidity was the best prognosis factor to predict survival probability of liver resected patieo1~suffering from COG. Patients with diploid CCC survived for a long time whereasaneuploidtumors indicateda poor prognosis with a rather short survival time. We conclude that DNA-ploidity is a valuable diagnostic tool for identifying subgroups of patients that may be at higher risk for tumor progression and might not benefit from liver resection.
*P
2783 2780
Clinical Utility Of Color Doppler Ultmcanography In Pediatric Liver TrausplantaUon From Living Donors Masazumi None Fujimoto, Shiga Medical Ctr for Adult Diseases, Moriyama-shi, Shiga Japan; Kazuhiko None Mizuta, Shiga Medical Ctr, Moriyama-shi, Shiga Japan; Fuminori None Moriyasu, Tokyo Medical Univ, Shinjuku-ku, Tokyo Japan; Tsutomu None Chiba, Koichi None Tanaka, Kyoto Univ Fac of Med, Kyoto-shi, Kyoto Japan
Oral Administration of Upopolysaccharides Synergically Enhances Liver injury Caused by Chronic Ethanol Feeding in Rats - A New Animal Model of Alcoholic Hepatitis Hironao Tamai, Yoshinori Horie, Shinzo Kato, Eiji Ohki, Hiromasa Ishii, Keio Univ Sch of Medicine, Tokyo Japan Background and aims: Endotoxin has been implicated in the pathogenesisand progression of alcoholic liver disease. However, it is still unclear how chronic ethanol feeding affects absorption of endotoxin from the intestine, and susceptibility of the liver to gut-derived endotoxin. The aim of this study was to determine the effect of chronic ethanol feeding on susceptibility of the liver to orally administeredendotoxin. Methods: Male Wistar rats weighing about 150g were pair fed with ethanol containing liquid diet or control diet for 5 weeks. In another set of animal feeding, 0,10 or 20 mg/kg of tipopolysaccharides(LPS) were added to the liquid diet for 7days beginning on Day 29~. On Day 3P, the animals were sacrificed for blood biochemistry and histological examination of the liver. Plasma endotoxin levels were also determined after 20mg/kg LPS administration using a gastric tube. In another set of experiments, we determined intestinal permeability using FD4 (fluorescein isothiocyanatelabeled dextran with an averagemolecular weight 4000 D). Results: With 10 mg/kg of LPS, serum ALT and ALP levels were significantly increased in ethanol fed rats, which were not noted in the controls. After 20 mg/kg of LPS administration, more substantial increases in serum ALT and ALP levels were observed in ethanol-fed rats compared to pair-fed rats. Neutrophil infiltration, ballooning and spotty necrosis were prominent in rats given LPS in ethanol containing diets. Plasmaendotoxin levels in chronic ethanol-fed rats were higher than those in pair-fed rats. Furthermore, the intestinal permeability to FD4 was increased by chronic ethanol administration. Conclusion:Theseresults suggestthat chronic ethanolfeeding increasesthe intestinal permeabilityand absorption of endotoxin, resulting in an enhancement of the susceptibility of the liver to orally administeredendotoxin.This model servesa subclinical experimental model for the study of alcoholic liver disease. 2781 Change Of Hepatic EnzymesAfter Acute Alcohol intake in Non-Soffish Volunteers Youming Li, Min Yue, Chaohui Yu, Weixing Chen, Gengyun Xu, 1st Affiliated Hosp, Hangzhou People'S Rep Of China Objectives:Changesin variable hepatic functional marks after acute alcohol intake in nonsottish volunteers are discussed to clarify the influence of acute alcohol intake on liver and the importance of variable marks under different blood alcohol level and time points. Methods:Affer 15 volunteers have drunken 182mL white wine which contains 80g alcohol in half an hour, blood samples are taken at 8 intervals while 0.5,1,1.5,2,2.5,3,6,24hours from the very beginningto detect ALT,AST,NAPand ~t-GT.Meanwhilethe blood alcohol level, heart rate, blood pressure and mental state are inspected. Results:The ratio of alt/ast increases after single alcohol intake while the blood alcohol level is higher than 1.2g/L with P value less than 0.05. NAP and ~/-GT also increase while the blood level is less than O.4g/L with p value less than 0.01 and 0.0001. No marks make significant changes on different time points. Conclusions: We may predict that acute alcoholism may induce the changes of liver enzymic system and this supports the multiple organic injury of alchhol.
Using color Doppler US, we examined hepatic blood flow to assess vascular anastomosis intraoperatively and for postoperatve monitoring of graft circulation in 122 pediatric liver transplants from living donors. Our examinationsfocused on (1) peak systolic velocity (Vp) and pulsatility index (PI) of the hepaticartery, (2) blood flow in the portal vein and spontaneous portosystemic collateral pathways, and (3) flow velocity and waveforms in the hepatic vein. (1) Six cases with hepatic artery thrombosis were detected by Doppler US. In 2 cases with dampened waveforms at intraoperative flowmetry (Vp-<23 cm/s and PI-
2782 DNA-ploidlty Predicts Clinical OutcomeAfter Liver Resection Due to Cholangiocarcinoma Hassan Abou-Rebyeh, Dept Internal Medicine, Hepatologyand Gastroenterology,Berlin Germany; Hussain AI-Abadi, Sven Jonas, Inga Rotter, Dept Gen, Visceral and Transplantation Surg, Berlin Germany; Wilfded Voltzke-Schlieker,Andreas Adler, Rainer E. Hintze, Bertram Wiedenmann, Dept Internal Medicine, Hepatologyand Gastroenterology, Berlin Germany; Peter Neuhaus, Dept Gun, Visceral and Transplantation Surg, Berlin Germany Background: Many patients afflicted from cholangiocarcinoma (CCC) undergo partial liver resection. A variety of prognostic factors are used to predict their clinical outcome. So far, tumor stage, grade, stage of metastasis and completenessof tumor resection are used as prognostic factors to predict the outcome of CCC-patients after liver resection. But their predictive value seems to be limited. We investigated DNA-ploidity in terms of chromosomal set nnmber ol CCCtumor cell lines at time of operationwhich might be an alternativeparameter for prediction of survival probability. METHODS:We included 27 patients into a prospective
A-547