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Role of plasminogen activator inhibitor-1 (PAI-1) in DIC
Vol. 63, No. 2
ABSTRACTS;
CHINESE-JAPANESE
SYMP. 1990
269
THROMBOLYSIS AND HEALING PROCESS OF OF T-PA ON EFFECT MICROVASCULAR ANASTOMOSIS IN RAT B.Y.sa. Y.D.Gu. J-G.YWn. X.L.Wu. Shanghai Medical University, Shanghai, China Keeping the vascular patency after anastomosis is very important for microsurgery. We try to use t-PA for prevention and treatment of thrombosis to elevate the rate of tissue survival in microsurgery. Femoral artery of rat was exposed and the vessel Patency test was positive and thrombus could be wall was damaged. visible by naked eye in 20-30 min after anastomosis. Rats were divided into four groups. Each group was intravenously infused with t-PA (5mg/Kg), t-PA plus heparin, heparin (3OOu/Kg) and normal saline respectively after thrombosis. The healing process of endothelium was observed by scanning electromicroscope. The treatment with t-PA combined with continuous heparin infusion was Complete thrombolysis was observed in all very successful. thrombi (n=80) in lo-20 min with no significant decrease of plasma The repair of fibrinogen, but two of the vessels were reoccluded. In lo-14 days, the surface of endothelium started in 24 hrs. vessel wall was lined by endothelial cells. Infusion of t-PA alone, all the vessels were reoccluded. Infusion of heparin or normal saline alone, there was no thrombolysis at all. Plasma t-PA antigen or activity got highest level in 6 min after infusion of t-PA. The baseline of plasma fibrinogen was 199+38mg/dl, and decreased to 78f19mg/dl, after 1 hr of t-PA infusion and was increased to 260-300mg/dl in 24-48 hrs after infusion, in the meantime the extraneous t-PA was almost clearanced. ROLE OF PLASMINOGEN
ACTIVATOR INHIBITOR-l (PAI-1) IN DIC .I a. M. Salt-o. H. Jokali. C. Uot&.
I, . I I?. ; Department of Internal Medicine (III), Kanazawa University School of Medicine, Kanazawa 920, Japan In cases of disseminated intravascular coagulation (DIG), larger part of multiple thrombi developed in the small vessels in the whole body rapidly disappears by activation of fibrinolytic In cases of DIC with multiple organ failure (MOF), levels system. of free PAIactivity and t-PA-PAIcomplex in plasma were higher than in cases of DIC without MOF. However, in levels of thrombin-antithrombin III complex and plasmin-azantiplasmin between these two groups. complex, there were no differences These results suggest that role of PA1 is more important than formation of thrombin or plasmin, in the development of DIC. It is interesting that in vivo release of t-PA seems to increase in the patients with MOF, because level of t-PA-PAIcomplex elevated. In these cases, increase in PAI- may have overwhelmed increased release of t-PA, and have caused remaining of multiple thrombi which is important in development of disturbance of microcirculation and MOF.
ABSTRACTS;
CHINESE-JAPANESE
SYMP. 1990
269
THROMBOLYSIS AND HEALING PROCESS OF OF T-PA ON EFFECT MICROVASCULAR ANASTOMOSIS IN RAT B.Y.sa. Y.D.Gu. J-G.YWn. X.L.Wu. Shanghai Medical University, Shanghai, China Keeping the vascular patency after anastomosis is very important for microsurgery. We try to use t-PA for prevention and treatment of thrombosis to elevate the rate of tissue survival in microsurgery. Femoral artery of rat was exposed and the vessel Patency test was positive and thrombus could be wall was damaged. visible by naked eye in 20-30 min after anastomosis. Rats were divided into four groups. Each group was intravenously infused with t-PA (5mg/Kg), t-PA plus heparin, heparin (3OOu/Kg) and normal saline respectively after thrombosis. The healing process of endothelium was observed by scanning electromicroscope. The treatment with t-PA combined with continuous heparin infusion was Complete thrombolysis was observed in all very successful. thrombi (n=80) in lo-20 min with no significant decrease of plasma The repair of fibrinogen, but two of the vessels were reoccluded. In lo-14 days, the surface of endothelium started in 24 hrs. vessel wall was lined by endothelial cells. Infusion of t-PA alone, all the vessels were reoccluded. Infusion of heparin or normal saline alone, there was no thrombolysis at all. Plasma t-PA antigen or activity got highest level in 6 min after infusion of t-PA. The baseline of plasma fibrinogen was 199+38mg/dl, and decreased to 78f19mg/dl, after 1 hr of t-PA infusion and was increased to 260-300mg/dl in 24-48 hrs after infusion, in the meantime the extraneous t-PA was almost clearanced. ROLE OF PLASMINOGEN
ACTIVATOR INHIBITOR-l (PAI-1) IN DIC .I a. M. Salt-o. H. Jokali. C. Uot&.
I, . I I?. ; Department of Internal Medicine (III), Kanazawa University School of Medicine, Kanazawa 920, Japan In cases of disseminated intravascular coagulation (DIG), larger part of multiple thrombi developed in the small vessels in the whole body rapidly disappears by activation of fibrinolytic In cases of DIC with multiple organ failure (MOF), levels system. of free PAIactivity and t-PA-PAIcomplex in plasma were higher than in cases of DIC without MOF. However, in levels of thrombin-antithrombin III complex and plasmin-azantiplasmin between these two groups. complex, there were no differences These results suggest that role of PA1 is more important than formation of thrombin or plasmin, in the development of DIC. It is interesting that in vivo release of t-PA seems to increase in the patients with MOF, because level of t-PA-PAIcomplex elevated. In these cases, increase in PAI- may have overwhelmed increased release of t-PA, and have caused remaining of multiple thrombi which is important in development of disturbance of microcirculation and MOF.