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Role of prostaglandins in oleic acid and secretin mediated inhibition of human gastric acid secretion
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ENRICHMENT OF GASTRIC ENDOCRINE CELLS BY FREE FLOW ELECTROPHORESIS. Theurer D, Hoerer W, Weber G, L i e b i c h H*,Teichmann RK, Becker HD. Dept. of Surgery, Univ. of Tuebingen, end I n s t . f o r Animal Embryol. and H i s t o l . , Univ. of Munich*,FRG. Separation of endocrine c e l l s of the g a s t r o i n t e s t i n a l t r a c t i s an issue. The purpose o f the present study was to demonstrate an enrichment of v i t a l endocrine c e l l s of the antrum using ~ free f l o w e l e c t r o p h o r e s i s . Method. A n t r a l c e l l suspension s (10- c e l l s / m l b u f f e r ) were prepared e n z y m e t i c a l l y from pigs (n=12). The suspension was t r a n s f e r r e d on a f r e e f l o w e l e c t r o p h o r e s i s chamber. N i n e t y f r a c t i o n s were c o l l e c t e d and analysed f o r c e l l s by l i g h t - and electronmicroscopy as w e l l as f o r g a s t r i n s t i m u l a t i o n by bombesin. Results. C e l l s were c l a s s i f i e d i n t o v i t a l endocrine, lymphoid, e p i t h e l i a l c e l l s and fragments. Endocrine c e l l s were found i n 5 f r a c t i o n s . The percentages were 2,8 ( f r a c t i o n - 2 ) , 17,3 ( - 1 ) , 37,1 (0), 21,3 (+1) and 10,5 % (+2). In the f r a c t i o n 0 ( h i g h e s t pecentage of endocrine c e l l s ) 2 7 , 1 % lymphoid and 23 % e p i t h e a l c e l l s were found i n a d d i t i o n . I n c u b a t i o n of f r a c t i o n 0 w i t h bombesin caused a s t i m u l a t i o n of g a s t r i n release. Conclusion. Free f l o w e l e c t r o p h o r e s i s seems to be a u s e f u l t o o l f o r s i g n i f i c a n t enrichment of v i t a l endocrine c e l l s of the antrum.
MDRPHOLOGICALCHARACTERISTICS OF ENTERIC NEURONS P R Y I N G FROM THE SMALL INTESTINE ANDCOLON TO THECRANIALMESENTERICGANGLION OF THE PIG. Timmermans J-P, Scheuermann DW, Barbiers M, Adriaensen D, Stach W', De Groodt-Lasseel MHA. Institute of Histology and Microscopic Anatomy, University
Centre Antwerp, B-2020 Antwerp, Belgium. ~Institute of ~natomy, University of Rostock, Germany.
In order to determine the occurrence and distribution of enteric neurons projecting to the cranial mesenteric ganglion and to reveal their neurotransmitter content, in vivo retrograde tracing experiments using Fluorogold or Fast Blue as a neuronal marker were carried out in combination with immunocytochemistry. 3-4 week old domestic pigs were used in this study. 2-4 weeks after injection of the tracer, the animals, being pretreated with colchicine 5 hours before, were killed and immunocytochemistry was performed. The number of retrograaely labellea neurons increase zrom the proximal to the caudal region of the intestinal tract. Nezertheless, the highest density is found at the end of the ascending colon. Traced neurons occur mainly in ganglia of the myenteric and outer submucous plexus found in a narrow rim on either side of the mesenteric attachment. Both small intestinal and colonic retrogradely labelled neurons have a multidendritic appearance. Part of the Fluorogold- or Fast Blue-labelled enteric neurons display immunoreactivity for serotonin. In previous reports on the porcine small intestine we already identified serotonergic neurons as morphological type III neurons Isee Timmermans et al. J. Auton. Nerv. System 33, (]93-194), ]990; Scheuermann et al. Anat. Anz. Suppl. 170, (75-85), 1991). The observation that in the small intestine part of the retrogradely labelled neurons, which analoqously resemble type III neurons, are serotonergic, is in agreement wit~ their proposed interneuronal [ole. These findings indicate that serotonergic neurons participate in the complex mecnanisms regulating the excnange ana integration of information from distant regions of the intestinal tract via intestino-sympathical pathways that converge to the cranial mesenteric ganglion. Comparison of the aata of the current investigation with the avallable literature allows tO concluae tnat tne morpnological ana neurochemical features of the intestinofugally projecting neurons are species-dependent.
ENRICHMENT OF GASTRIC ENDOCRINE CELLS BY FREE FLOW ELECTROPHORESIS. Theurer D, Hoerer W, Weber G, L i e b i c h H*,Teichmann RK, Becker HD. Dept. of Surgery, Univ. of Tuebingen, end I n s t . f o r Animal Embryol. and H i s t o l . , Univ. of Munich*,FRG. Separation of endocrine c e l l s of the g a s t r o i n t e s t i n a l t r a c t i s an issue. The purpose o f the present study was to demonstrate an enrichment of v i t a l endocrine c e l l s of the antrum using ~ free f l o w e l e c t r o p h o r e s i s . Method. A n t r a l c e l l suspension s (10- c e l l s / m l b u f f e r ) were prepared e n z y m e t i c a l l y from pigs (n=12). The suspension was t r a n s f e r r e d on a f r e e f l o w e l e c t r o p h o r e s i s chamber. N i n e t y f r a c t i o n s were c o l l e c t e d and analysed f o r c e l l s by l i g h t - and electronmicroscopy as w e l l as f o r g a s t r i n s t i m u l a t i o n by bombesin. Results. C e l l s were c l a s s i f i e d i n t o v i t a l endocrine, lymphoid, e p i t h e l i a l c e l l s and fragments. Endocrine c e l l s were found i n 5 f r a c t i o n s . The percentages were 2,8 ( f r a c t i o n - 2 ) , 17,3 ( - 1 ) , 37,1 (0), 21,3 (+1) and 10,5 % (+2). In the f r a c t i o n 0 ( h i g h e s t pecentage of endocrine c e l l s ) 2 7 , 1 % lymphoid and 23 % e p i t h e a l c e l l s were found i n a d d i t i o n . I n c u b a t i o n of f r a c t i o n 0 w i t h bombesin caused a s t i m u l a t i o n of g a s t r i n release. Conclusion. Free f l o w e l e c t r o p h o r e s i s seems to be a u s e f u l t o o l f o r s i g n i f i c a n t enrichment of v i t a l endocrine c e l l s of the antrum.
MDRPHOLOGICALCHARACTERISTICS OF ENTERIC NEURONS P R Y I N G FROM THE SMALL INTESTINE ANDCOLON TO THECRANIALMESENTERICGANGLION OF THE PIG. Timmermans J-P, Scheuermann DW, Barbiers M, Adriaensen D, Stach W', De Groodt-Lasseel MHA. Institute of Histology and Microscopic Anatomy, University
Centre Antwerp, B-2020 Antwerp, Belgium. ~Institute of ~natomy, University of Rostock, Germany.
In order to determine the occurrence and distribution of enteric neurons projecting to the cranial mesenteric ganglion and to reveal their neurotransmitter content, in vivo retrograde tracing experiments using Fluorogold or Fast Blue as a neuronal marker were carried out in combination with immunocytochemistry. 3-4 week old domestic pigs were used in this study. 2-4 weeks after injection of the tracer, the animals, being pretreated with colchicine 5 hours before, were killed and immunocytochemistry was performed. The number of retrograaely labellea neurons increase zrom the proximal to the caudal region of the intestinal tract. Nezertheless, the highest density is found at the end of the ascending colon. Traced neurons occur mainly in ganglia of the myenteric and outer submucous plexus found in a narrow rim on either side of the mesenteric attachment. Both small intestinal and colonic retrogradely labelled neurons have a multidendritic appearance. Part of the Fluorogold- or Fast Blue-labelled enteric neurons display immunoreactivity for serotonin. In previous reports on the porcine small intestine we already identified serotonergic neurons as morphological type III neurons Isee Timmermans et al. J. Auton. Nerv. System 33, (]93-194), ]990; Scheuermann et al. Anat. Anz. Suppl. 170, (75-85), 1991). The observation that in the small intestine part of the retrogradely labelled neurons, which analoqously resemble type III neurons, are serotonergic, is in agreement wit~ their proposed interneuronal [ole. These findings indicate that serotonergic neurons participate in the complex mecnanisms regulating the excnange ana integration of information from distant regions of the intestinal tract via intestino-sympathical pathways that converge to the cranial mesenteric ganglion. Comparison of the aata of the current investigation with the avallable literature allows tO concluae tnat tne morpnological ana neurochemical features of the intestinofugally projecting neurons are species-dependent.