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Rose odor counteract the stress-induced effect of the predator odor
Abstracts
S211
structure of the organized network corresponds to the computational algorithm for odor representation. Thus, the result indicates that the representation algorithm in our model can be implemented into the network in an unsupervised manner.
lar Ca2+ was elicited. The result suggests these TB lines can respond to sour taste stimuli.
Reference
P3-c23 Analysis of electrical oscillation patterns of the olfactory center of land slugs Yu-ta Hamasaki 1 , Yuu Kumai 1 , Katsunori Tanaka 1 , Yoshimasa Komatuzaki 2 , Minoru Saito 1
Makino, et al., 2007. Neurosci. Res. 58, S103. Makino, et al., 2008. Neurosci. Res. 61, S249.
1
doi:10.1016/j.neures.2009.09.1164
2
P3-c20 Histological difference between V1R- and V2R-glomerulus in the mouse accessory olfactory bulb Makoto Yokosuka 1 , Kaoruko Hashimoto 1 , Tomoaki Nakada 1 , Toru Saito 1 , Masumi Ichikawa 2 1
Nippon Vet Life Sci Univ, Tokyo, Japan; Japan
2
Tokyo Met Ins Neurosci, Tokyo,
Vomeronasal receptors (VRs) in mammals are classified in two families, V1R and V2R. Rodent has both receptor families and they receive the different type of chemicals. However, it is unknown how these two receptor families induce different physiological functions. In rodents, axon of the V1R- and V2R cells projects to the anterior half- and posterior half- of accessory olfactory bulb (AOB), respectively. Moreover, each receptor axon terminals form several numbers of glomerulus, independently. For the purpose of understanding the central circuit of vomeronasal system, we demonstrate histological difference of the glomerulus form between V1R and V2R of the mouse AOB. Glomerulus size and the number of Juxtaglomerular cell corresponding to each glomerulus were significantly difference between V1R and V2R. These results suggest the possibility that the different roles between V1R and V2R occurs not only the difference of ligands, but also the difference of the synaptic circuit in the glomerulus of the AOB. doi:10.1016/j.neures.2009.09.1165
Dept Funct Morphol, Nihon Univ Sch Med, Tokyo, Japan; Engineering, AIST, Hyogo, Japan
Graduate School of Integrated Basic Sciences,Nihon University, Japan; College of Science and Technology, Nihon University, Japan
We examined the odor responses of oscillatory activity in the olfactory center (procerebrum; PC) of the land slug Limax by extracellular recording. The PC was isolated together with the tentacle and the carrot or cucumber odor was applied only to the tentacle. The local field potential (LFP) of the PC was recorded through a suction electrode filled with the saline and sampled with 1 kHz. Prior to the odor stimulus, the LFP showed an oscillation of about 0.5–1 Hz. The continuous exposure of each odor to the tentacle slightly increased the frequency. Then we tried to reconstruct the attractor from the time series of each LFP. The result showed the different characters in the attractors. We further examined the odor responses of the slugs subjected to aversive training. In the trained slugs, the bursting oscillations of LFP were induced by the conditioned odor after several minutes from the exposure. The reconstructed attractors and Wavelet analysis showed a characteristic onset to the bursting oscillations. doi:10.1016/j.neures.2009.09.1168
P3-c24 Taste response of rat hippocampal neurons in different places Takashi Kondoh 1 , Anh S. Ho 2,3 , Esturo Hori 2,3 , Phuong H.T. Nguyen 2,3 , Susumu Urakawa 2,3 , Kunio Torii 1 , Taketoshi Ono 2,3 , Hisao Nishijo 2,3 1
P3-c21 Rose odor counteract the stress-induced effect of the predator odor Mutsumi Matsukawa 1 , Masato Imada 1 , Takaaki Sato 2 1
doi:10.1016/j.neures.2009.09.1167
2
Res Inst Cell
For rodents, the predator odor such as TMT, has been known to activate HPAaxis which causes stress-like behavior. Recently, the medial part of the bed nucleus of stria terminalis (mBST) has shown to be involved in this process. However, it remains unclear that there is any odor which counteract to predator odor they have never experienced before. In this study, we tried to examine rose odor, which is novel odor for animals, to have an effect on relax or not. We counted the number of immunohistochemically identified cells using antibody against cFos in the mice brain following presentation of odors during 30 min before perfusion fixation. Results were shown that cFos immunopositive cells in the mBST were significantly reduced by a mixture of TMT and rose odor. It suggest that the rose odor may have an effect against the predator odor-induced stress without any experience. doi:10.1016/j.neures.2009.09.1166
P3-c22 Analysis of taste reception capability of clonal cell lines derived from murine taste buds with imaging system of intracellular calcium Miyako Nishiyama 1 , Hideyuki Sako 2 , Kayoko Hasizume 1 , Takenori Miyamoto 1 , Yasuhiro Tomooka 2 1 Div. Mater. Biol. Sci., Grad. Sch. Sci., Japan Women’s University, Tokyo, Japan; 2 Department Biol. Sci. and Tech., Grad. Sch. Ind. Sci. and Tech., Tokyo University of Sci., Chiba, Japan
Many researchers tried to isolate taste buds and develop in vitro systems. In our laboratory, we collected taste buds from a murine tongue and established clonal cell lines (TB lines). Expression patterns of some taste receptors in four TB lines, TB-a1, TB-a5, TB-a7 and TB-c1 were examined with RT-PCR. All of them expressed T1R3 for sweet and umami taste, and T2R8 for bitter taste. A candidate salty receptor ␣-ENaC was also expressed in the four lines. While, a candidate sour receptor HCN4 was expressed in TB-a1 and TB-a7 lines, and another candidate receptor PKD1L3 was slightly expressed in TB-a1 and TB-c1 lines. Hence, to examine whether TB lines can response to taste stimuli, we performed calcium-imaging analyses. Some of TB lines responded to citric acid, and a transient elevation of intracellu-
Inst Life Sci, Ajinomoto Co, Inc, Kawasaki, Japan; 2 System Emotional Sci, Univ of Toyama, Toyama, Japan; 3 CREST, JST, Japan
Hippocampal formation (HF) receives information from all sensory modalities. We recorded CA1 HF neurons from freely behaving rats during performance of a visually-guided licking task in 2 different places (white or black chambers). When a cue lamp on, rats were required to press a bar to trigger a tube to protrude into the chambers for 3 s. During this period, the rats could lick water or one of 6 sapid solutions [NaCl, sucrose, citric acid, quinine, monosodium L-glutamate (MSG), and a mixture of MSG with 5 -inosinate]. Of a total of 285 pyramidal and theta neurons recorded, 137 neurons responded during licking, and responses of 62 neurons were greater to sapid solutions than to water (taste neurons). Multivariate analyses suggested that taste quality in the HF might be encoded based on hedonic value. Furthermore, the activity of most taste neurons was chamber-specific. These results implicate the HF in guiding appetitive behaviors such as conditioned place preference. doi:10.1016/j.neures.2009.09.1168
P3-c25 Conserved orthologous vomeronasal type 1 receptor genes among goat, sheep and cow Hiromi Ohara 1 , Masato Nikaido 2 , Atsuko Date-Ito 1,3 , Kazutaka Mogi 3,4 , Hiroaki Okamura 4 , Norihiro Okada 2 , Yukari Takeuchi 3 , Yuji Mori 3 , Kimiko Hagino-Yamagishi 1 1
Tokyo Metropolitan Institute of Medical Sci, Tokyo, Japan; 2 Grad School of Biosci and Biotechnol, Tokyo Inst of Technol, Yokohama, Japan; 3 Grad School of Agri Life Sci, Univ of Tokyo, Tokyo; 4 National Institute of Agrobiol Sci, Tukuba, Japan Pheromones have been generally thought to have species-specific functions, and the diverged repertoires of the V1Rs, putative pheromone receptors, suggest that V1Rs contributes to species-specific recognition of pheromones in rodents. However, the goat male pheromone, which can induce out-of-season ovulation in anestrous females, cause the same pheromone response in sheep, and vice versa, suggesting that there may be mechanisms for detecting “inter-species” pheromones among ruminant species. Then, we investigated ruminant V1R genes, and found that the sequence identity of V1R orthologous pairs/sets among these ruminants are quite conserved as compared with that to the mouse-rat orthologous pairs. We predict that these orthologous V1Rs can detect the same or closely related chemicals within each orthologous pair/set. doi:10.1016/j.neures.2009.09.1170
S211
structure of the organized network corresponds to the computational algorithm for odor representation. Thus, the result indicates that the representation algorithm in our model can be implemented into the network in an unsupervised manner.
lar Ca2+ was elicited. The result suggests these TB lines can respond to sour taste stimuli.
Reference
P3-c23 Analysis of electrical oscillation patterns of the olfactory center of land slugs Yu-ta Hamasaki 1 , Yuu Kumai 1 , Katsunori Tanaka 1 , Yoshimasa Komatuzaki 2 , Minoru Saito 1
Makino, et al., 2007. Neurosci. Res. 58, S103. Makino, et al., 2008. Neurosci. Res. 61, S249.
1
doi:10.1016/j.neures.2009.09.1164
2
P3-c20 Histological difference between V1R- and V2R-glomerulus in the mouse accessory olfactory bulb Makoto Yokosuka 1 , Kaoruko Hashimoto 1 , Tomoaki Nakada 1 , Toru Saito 1 , Masumi Ichikawa 2 1
Nippon Vet Life Sci Univ, Tokyo, Japan; Japan
2
Tokyo Met Ins Neurosci, Tokyo,
Vomeronasal receptors (VRs) in mammals are classified in two families, V1R and V2R. Rodent has both receptor families and they receive the different type of chemicals. However, it is unknown how these two receptor families induce different physiological functions. In rodents, axon of the V1R- and V2R cells projects to the anterior half- and posterior half- of accessory olfactory bulb (AOB), respectively. Moreover, each receptor axon terminals form several numbers of glomerulus, independently. For the purpose of understanding the central circuit of vomeronasal system, we demonstrate histological difference of the glomerulus form between V1R and V2R of the mouse AOB. Glomerulus size and the number of Juxtaglomerular cell corresponding to each glomerulus were significantly difference between V1R and V2R. These results suggest the possibility that the different roles between V1R and V2R occurs not only the difference of ligands, but also the difference of the synaptic circuit in the glomerulus of the AOB. doi:10.1016/j.neures.2009.09.1165
Dept Funct Morphol, Nihon Univ Sch Med, Tokyo, Japan; Engineering, AIST, Hyogo, Japan
Graduate School of Integrated Basic Sciences,Nihon University, Japan; College of Science and Technology, Nihon University, Japan
We examined the odor responses of oscillatory activity in the olfactory center (procerebrum; PC) of the land slug Limax by extracellular recording. The PC was isolated together with the tentacle and the carrot or cucumber odor was applied only to the tentacle. The local field potential (LFP) of the PC was recorded through a suction electrode filled with the saline and sampled with 1 kHz. Prior to the odor stimulus, the LFP showed an oscillation of about 0.5–1 Hz. The continuous exposure of each odor to the tentacle slightly increased the frequency. Then we tried to reconstruct the attractor from the time series of each LFP. The result showed the different characters in the attractors. We further examined the odor responses of the slugs subjected to aversive training. In the trained slugs, the bursting oscillations of LFP were induced by the conditioned odor after several minutes from the exposure. The reconstructed attractors and Wavelet analysis showed a characteristic onset to the bursting oscillations. doi:10.1016/j.neures.2009.09.1168
P3-c24 Taste response of rat hippocampal neurons in different places Takashi Kondoh 1 , Anh S. Ho 2,3 , Esturo Hori 2,3 , Phuong H.T. Nguyen 2,3 , Susumu Urakawa 2,3 , Kunio Torii 1 , Taketoshi Ono 2,3 , Hisao Nishijo 2,3 1
P3-c21 Rose odor counteract the stress-induced effect of the predator odor Mutsumi Matsukawa 1 , Masato Imada 1 , Takaaki Sato 2 1
doi:10.1016/j.neures.2009.09.1167
2
Res Inst Cell
For rodents, the predator odor such as TMT, has been known to activate HPAaxis which causes stress-like behavior. Recently, the medial part of the bed nucleus of stria terminalis (mBST) has shown to be involved in this process. However, it remains unclear that there is any odor which counteract to predator odor they have never experienced before. In this study, we tried to examine rose odor, which is novel odor for animals, to have an effect on relax or not. We counted the number of immunohistochemically identified cells using antibody against cFos in the mice brain following presentation of odors during 30 min before perfusion fixation. Results were shown that cFos immunopositive cells in the mBST were significantly reduced by a mixture of TMT and rose odor. It suggest that the rose odor may have an effect against the predator odor-induced stress without any experience. doi:10.1016/j.neures.2009.09.1166
P3-c22 Analysis of taste reception capability of clonal cell lines derived from murine taste buds with imaging system of intracellular calcium Miyako Nishiyama 1 , Hideyuki Sako 2 , Kayoko Hasizume 1 , Takenori Miyamoto 1 , Yasuhiro Tomooka 2 1 Div. Mater. Biol. Sci., Grad. Sch. Sci., Japan Women’s University, Tokyo, Japan; 2 Department Biol. Sci. and Tech., Grad. Sch. Ind. Sci. and Tech., Tokyo University of Sci., Chiba, Japan
Many researchers tried to isolate taste buds and develop in vitro systems. In our laboratory, we collected taste buds from a murine tongue and established clonal cell lines (TB lines). Expression patterns of some taste receptors in four TB lines, TB-a1, TB-a5, TB-a7 and TB-c1 were examined with RT-PCR. All of them expressed T1R3 for sweet and umami taste, and T2R8 for bitter taste. A candidate salty receptor ␣-ENaC was also expressed in the four lines. While, a candidate sour receptor HCN4 was expressed in TB-a1 and TB-a7 lines, and another candidate receptor PKD1L3 was slightly expressed in TB-a1 and TB-c1 lines. Hence, to examine whether TB lines can response to taste stimuli, we performed calcium-imaging analyses. Some of TB lines responded to citric acid, and a transient elevation of intracellu-
Inst Life Sci, Ajinomoto Co, Inc, Kawasaki, Japan; 2 System Emotional Sci, Univ of Toyama, Toyama, Japan; 3 CREST, JST, Japan
Hippocampal formation (HF) receives information from all sensory modalities. We recorded CA1 HF neurons from freely behaving rats during performance of a visually-guided licking task in 2 different places (white or black chambers). When a cue lamp on, rats were required to press a bar to trigger a tube to protrude into the chambers for 3 s. During this period, the rats could lick water or one of 6 sapid solutions [NaCl, sucrose, citric acid, quinine, monosodium L-glutamate (MSG), and a mixture of MSG with 5 -inosinate]. Of a total of 285 pyramidal and theta neurons recorded, 137 neurons responded during licking, and responses of 62 neurons were greater to sapid solutions than to water (taste neurons). Multivariate analyses suggested that taste quality in the HF might be encoded based on hedonic value. Furthermore, the activity of most taste neurons was chamber-specific. These results implicate the HF in guiding appetitive behaviors such as conditioned place preference. doi:10.1016/j.neures.2009.09.1168
P3-c25 Conserved orthologous vomeronasal type 1 receptor genes among goat, sheep and cow Hiromi Ohara 1 , Masato Nikaido 2 , Atsuko Date-Ito 1,3 , Kazutaka Mogi 3,4 , Hiroaki Okamura 4 , Norihiro Okada 2 , Yukari Takeuchi 3 , Yuji Mori 3 , Kimiko Hagino-Yamagishi 1 1
Tokyo Metropolitan Institute of Medical Sci, Tokyo, Japan; 2 Grad School of Biosci and Biotechnol, Tokyo Inst of Technol, Yokohama, Japan; 3 Grad School of Agri Life Sci, Univ of Tokyo, Tokyo; 4 National Institute of Agrobiol Sci, Tukuba, Japan Pheromones have been generally thought to have species-specific functions, and the diverged repertoires of the V1Rs, putative pheromone receptors, suggest that V1Rs contributes to species-specific recognition of pheromones in rodents. However, the goat male pheromone, which can induce out-of-season ovulation in anestrous females, cause the same pheromone response in sheep, and vice versa, suggesting that there may be mechanisms for detecting “inter-species” pheromones among ruminant species. Then, we investigated ruminant V1R genes, and found that the sequence identity of V1R orthologous pairs/sets among these ruminants are quite conserved as compared with that to the mouse-rat orthologous pairs. We predict that these orthologous V1Rs can detect the same or closely related chemicals within each orthologous pair/set. doi:10.1016/j.neures.2009.09.1170