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Rotary evaporator
Rotary evaporator
T r a n s f e c t e d cell s e l e c t i o n Mihenyi
I ira =rn~i
Bioteeh have introduced the
new MACSelect system fi~r last and easy magnetic isolation of stablx and transiently transfcctcd cells. A pMA(:S plasmid encoding a cell surface marker is cotransfccted with the plasmid of interest by any standard transfcction method. After a short incubation time transfected cells can be enriched to high purity using the colloidal XIACSclect MicroBeads. Transfectants can be isolated and anal\zed t'rnm
Table top centrifuge
f o r m e r l y n n t r a n s f e c t a b l e cells, stlch as m o s t p r i m a r y cell lines. T h e s y s t e m is
Safelab Systems Ltd have introduced the TOM-VAP TM and POWERVAP TM rotary evaporators, which have Teflon/ground glass rotating seals to provide cost- and energy-efficient continuous solvent rccoxcrv t'cir mallv \ears. T()XI-\'AprM's rotary seal is guaranteed for 5 Tears and xxhen upgraded to POXVERVAP TM, its computer will run it 24 hours a day, automatically refilling its rotating flasks and emptying its distillate flasks. T h e floating-flask system ensures that a 20litrc POWERVAP TM can recover 1000 litrcs of solvent in 24 hours. Highpurity solvent recovery is possible due to the all glass and P T F E construction. Applications range from HPL(] and PREP L(] to histopathoh~g 3.
compatible with all conllllOnl\ tised transfcction methods and a wide range of cells from different species and tissues. Circle number 8 on a reader response card. Rotary mechanical stage
N e w fluorescence attachment Ensuring superb clarity and contrast, the new fluorescence attachment for the Eclipse
E600/E400 by Nikon Europe excels at
T h e PCR-SSP technique for HLA typing is based on the principle that a perfectly matched primer will be more efficiently used in the PCR reaction than a primer with several mismatches. T h e discrimination betx~ con alleles is part of the amplification step and post-PCR handling time is reduced to 30 minutes. T h e total time required from sample to restllt is less than 2 hours and 30 minutes, all with the same PCR profile. Four new products covering the I)Q groups, I)QBI~()2, ~03, ~04, ~()5 and '*06, are available now.
A n e w rotating stage with ceramic coating has been introduced fl)r the Carl Zeiss Axiolab routine laboratory microscopc. This increases the flexibility of the range, particularly whcrc specimens need to bc rotated for photonficrography or ~idco microscopy applications. Rotation is through 2 1 0 and the stage can bc locked in s#u at any angle. T o allow precise final positioning, there arc coaxial controls for the XY movement of the specimen and vernier scales for basic location. For improved longevity, the surface of the stage has been hardanodised and then ceramic-coated. This reduces scratcl]j~:tg of the 'Ill anodiscd surface anu, as it reduces resistance when the slide is placed into position in the specimen holder, it also improves case of use and user comfort.
Circle number 7 on a reader response card.
Circle number 9 on a reader resDonse card.
Circle number 6 on a reader response card.
High resolution PCR-SSP
Biofuge stratos, the new refrigerated, high speed, table-top centrifuge from Heraeus, incorporates functions normally only available on floor-standing units. A new drive spindle enables the Biofuge stratos to accelerate to a maximum RCF of 45,000 x g in 25 seconds. The spindle accepts an extensive range of rotors, including fixed angle, microlitre and swing-out plus a special rotor for microtitre plates, covering a wide range of temperature critical separation tasks. An imbalance system immediately detects any inbalance caused during a run, e.g. due to tube breakage. Circle number 10 on a reader response card.
handling the most demanding fluorescence tasks. Up to four filter cubes can be accommodated in any combination - for example one multi-bank filter plus three corresponding single-band filters. This quick slider mechanism also enables the selection of as few as two filter blocks by pulling the filter lever out one clock stop. Circle number 11 on a reader response card.
Screen saver To celebrate the launch of their new thermal cycler, PCR Express, Hybaid have produced a screen saver available for Mac and Windows formats. The screen saver features Eddie CoN (E. coh) in a mini cartoon with PCR Express, and can be downloaded free of charge from the Hybaid internet site (http://www.hybaid.co.uk). Circle number 12 on a reader response card.
Eukaryote cell transfection New T f x ~ - 1 0 TfxT~-20 reagents have been teamed with Promega's popular TfxTM--50 reagent to provide a trio of transfection reagents. The Tfx TM that performs best with your cell line can be chosen. The reagents offer high-efficiency transfection of several types of eukaryotic cells; transfection of DNA both in the presence or absence of serum in many cell lines; a simple, single-tube protocol; and short transfection time. Circle number 13 on a reader response card.
T r a n s f e c t e d cell s e l e c t i o n Mihenyi
I ira =rn~i
Bioteeh have introduced the
new MACSelect system fi~r last and easy magnetic isolation of stablx and transiently transfcctcd cells. A pMA(:S plasmid encoding a cell surface marker is cotransfccted with the plasmid of interest by any standard transfcction method. After a short incubation time transfected cells can be enriched to high purity using the colloidal XIACSclect MicroBeads. Transfectants can be isolated and anal\zed t'rnm
Table top centrifuge
f o r m e r l y n n t r a n s f e c t a b l e cells, stlch as m o s t p r i m a r y cell lines. T h e s y s t e m is
Safelab Systems Ltd have introduced the TOM-VAP TM and POWERVAP TM rotary evaporators, which have Teflon/ground glass rotating seals to provide cost- and energy-efficient continuous solvent rccoxcrv t'cir mallv \ears. T()XI-\'AprM's rotary seal is guaranteed for 5 Tears and xxhen upgraded to POXVERVAP TM, its computer will run it 24 hours a day, automatically refilling its rotating flasks and emptying its distillate flasks. T h e floating-flask system ensures that a 20litrc POWERVAP TM can recover 1000 litrcs of solvent in 24 hours. Highpurity solvent recovery is possible due to the all glass and P T F E construction. Applications range from HPL(] and PREP L(] to histopathoh~g 3.
compatible with all conllllOnl\ tised transfcction methods and a wide range of cells from different species and tissues. Circle number 8 on a reader response card. Rotary mechanical stage
N e w fluorescence attachment Ensuring superb clarity and contrast, the new fluorescence attachment for the Eclipse
E600/E400 by Nikon Europe excels at
T h e PCR-SSP technique for HLA typing is based on the principle that a perfectly matched primer will be more efficiently used in the PCR reaction than a primer with several mismatches. T h e discrimination betx~ con alleles is part of the amplification step and post-PCR handling time is reduced to 30 minutes. T h e total time required from sample to restllt is less than 2 hours and 30 minutes, all with the same PCR profile. Four new products covering the I)Q groups, I)QBI~()2, ~03, ~04, ~()5 and '*06, are available now.
A n e w rotating stage with ceramic coating has been introduced fl)r the Carl Zeiss Axiolab routine laboratory microscopc. This increases the flexibility of the range, particularly whcrc specimens need to bc rotated for photonficrography or ~idco microscopy applications. Rotation is through 2 1 0 and the stage can bc locked in s#u at any angle. T o allow precise final positioning, there arc coaxial controls for the XY movement of the specimen and vernier scales for basic location. For improved longevity, the surface of the stage has been hardanodised and then ceramic-coated. This reduces scratcl]j~:tg of the 'Ill anodiscd surface anu, as it reduces resistance when the slide is placed into position in the specimen holder, it also improves case of use and user comfort.
Circle number 7 on a reader response card.
Circle number 9 on a reader resDonse card.
Circle number 6 on a reader response card.
High resolution PCR-SSP
Biofuge stratos, the new refrigerated, high speed, table-top centrifuge from Heraeus, incorporates functions normally only available on floor-standing units. A new drive spindle enables the Biofuge stratos to accelerate to a maximum RCF of 45,000 x g in 25 seconds. The spindle accepts an extensive range of rotors, including fixed angle, microlitre and swing-out plus a special rotor for microtitre plates, covering a wide range of temperature critical separation tasks. An imbalance system immediately detects any inbalance caused during a run, e.g. due to tube breakage. Circle number 10 on a reader response card.
handling the most demanding fluorescence tasks. Up to four filter cubes can be accommodated in any combination - for example one multi-bank filter plus three corresponding single-band filters. This quick slider mechanism also enables the selection of as few as two filter blocks by pulling the filter lever out one clock stop. Circle number 11 on a reader response card.
Screen saver To celebrate the launch of their new thermal cycler, PCR Express, Hybaid have produced a screen saver available for Mac and Windows formats. The screen saver features Eddie CoN (E. coh) in a mini cartoon with PCR Express, and can be downloaded free of charge from the Hybaid internet site (http://www.hybaid.co.uk). Circle number 12 on a reader response card.
Eukaryote cell transfection New T f x ~ - 1 0 TfxT~-20 reagents have been teamed with Promega's popular TfxTM--50 reagent to provide a trio of transfection reagents. The Tfx TM that performs best with your cell line can be chosen. The reagents offer high-efficiency transfection of several types of eukaryotic cells; transfection of DNA both in the presence or absence of serum in many cell lines; a simple, single-tube protocol; and short transfection time. Circle number 13 on a reader response card.