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S274: Evaluation of protection of udenafil in rats with testicular ischemia-reperfusion damage
S274: Evaluation of protection of udenafil in rats with testicular ischemia-reperfusion damage Sertkaya Z.1, Öztürk M.1, Koca O.1, Akyüz M.1, Gümrükçü G.2, Kutluhan M.A.1, Karaman M.1 1
Haydarpaşa Numune Training And Research Hospital, Dept. of Urology, İstanbul, Turkey, Haydarpaşa Numune Training And Research Hospital, Dept. of Pathology, İstanbul, Turkey
2
INTRODUCTION & OBJECTIVES: Torsion of testicle, migration of neutrophiles to ischemic region and formation of free oxygen radicals are critical factors in formation of ischemic reperfusion damage (I/R) in testis. Udenafil is selective, strong and reversible inhibitor of cyclic guanosine monophosphate (cGMP)- specific phosphodiesterase type (PDE-5) enzyme in corpus cavernous. In our study our purpose was evaluation of protection of udenafil in reperfusion damage in testis as a result of I/R. MATERIAL & METHODS: Twenty one male adult Wistar-Albino rats with eighty months randomly divided into three groups; Sham, I/R, I/R + udenafil. While in sham and I/R groups intraperitoneal SF was given in second hour, in I/R+udenafil groups 1mg/kg intraperitoneal udenafil was given in detorsion. Spermatogenic factors was evaluated according to Johnsen criteria and mean scores was calculated for each testicle. While p value of 1,96 was considered statistically significant in multiple group comparison RESULTS: Histopathological and molecular parameters from all groups were compared after predicted torsion-detorsion times. Mean values of inflammatory mediators(TNF-a and IL-b) in venous blood samples were calculated. We observed that TNF-a values was statistically significant increased in I/R group than sham groups and udenafil treatment get these value to same level with sham groups by protecting of increase in TNF-value. We saw that glutathione peroxidase levels was statistically significant decreased in I/R group than sham group and treatment of udenafil avoided these decrease. GPx expression scores evaluated immunhistochemically was statistically significant increased in I/R group than sham groups and udenafil get these values to same levels with sham groups. In evaluation of spermatogenesis by using Johnsen scoring system there was no statistically significant difference in mean scores between groups. CONCLUSIONS: I/R as a result of T/D causes increase in inflammatory mediators in blood, increase in GPx activity, decrease in antioxidant capacity in tissue and no influence in spermatogenesis parameters for four hours. We can say that as a result deterioration of biochemical and histopathological parameters are reversed by isosorbid-5-mononitrate treatment. In other words udenafil decrease damage because of I/R in testicle. Results of these experimental study shows us that availability of udenafil treatment in testis torsion should be studied. Eur Urol Suppl 2014; 13(7) e1588
Haydarpaşa Numune Training And Research Hospital, Dept. of Urology, İstanbul, Turkey, Haydarpaşa Numune Training And Research Hospital, Dept. of Pathology, İstanbul, Turkey
2
INTRODUCTION & OBJECTIVES: Torsion of testicle, migration of neutrophiles to ischemic region and formation of free oxygen radicals are critical factors in formation of ischemic reperfusion damage (I/R) in testis. Udenafil is selective, strong and reversible inhibitor of cyclic guanosine monophosphate (cGMP)- specific phosphodiesterase type (PDE-5) enzyme in corpus cavernous. In our study our purpose was evaluation of protection of udenafil in reperfusion damage in testis as a result of I/R. MATERIAL & METHODS: Twenty one male adult Wistar-Albino rats with eighty months randomly divided into three groups; Sham, I/R, I/R + udenafil. While in sham and I/R groups intraperitoneal SF was given in second hour, in I/R+udenafil groups 1mg/kg intraperitoneal udenafil was given in detorsion. Spermatogenic factors was evaluated according to Johnsen criteria and mean scores was calculated for each testicle. While p value of 1,96 was considered statistically significant in multiple group comparison RESULTS: Histopathological and molecular parameters from all groups were compared after predicted torsion-detorsion times. Mean values of inflammatory mediators(TNF-a and IL-b) in venous blood samples were calculated. We observed that TNF-a values was statistically significant increased in I/R group than sham groups and udenafil treatment get these value to same level with sham groups by protecting of increase in TNF-value. We saw that glutathione peroxidase levels was statistically significant decreased in I/R group than sham group and treatment of udenafil avoided these decrease. GPx expression scores evaluated immunhistochemically was statistically significant increased in I/R group than sham groups and udenafil get these values to same levels with sham groups. In evaluation of spermatogenesis by using Johnsen scoring system there was no statistically significant difference in mean scores between groups. CONCLUSIONS: I/R as a result of T/D causes increase in inflammatory mediators in blood, increase in GPx activity, decrease in antioxidant capacity in tissue and no influence in spermatogenesis parameters for four hours. We can say that as a result deterioration of biochemical and histopathological parameters are reversed by isosorbid-5-mononitrate treatment. In other words udenafil decrease damage because of I/R in testicle. Results of these experimental study shows us that availability of udenafil treatment in testis torsion should be studied. Eur Urol Suppl 2014; 13(7) e1588