- Email: [email protected]
Salinity influence on cadmium phytoremediation capacity of the halophyte plants Salicornia ramosissima and Scirpus maritimus from coastal environments (Portugal)
S80
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
equilibrium characterization. The method of state functions constructing procedure basing on local state equations is implemented exploratory for this system. A larger study should be conducted for an extended aquatic system.
side, ethyl glucoside, 1-propanyl glucoside, 2-propanyl glucoside and butyl glucoside, respectively, because it was not detected in the reaction without alcohol. http://dx.doi.org/10.1016/j.copbio.2013.05.230
http://dx.doi.org/10.1016/j.copbio.2013.05.227 Salinity influence on cadmium phytoremediation capacity of the halophyte plants Salicornia ramosissima and Scirpus maritimus from coastal environments (Portugal) Marcia Santos 1 , Carmen Ferreira 3
Pedro 1 , Silvia
Gonc¸alves 2 , Susana
1
GIRM – Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Campus 4, Santuário Na. Sra. dos Remédios, 2520-641 Peniche, Portugal 2 IMAR – Marine and Environmental Research Centre, Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Largo Marquês de Pombal, 3004-517 Coimbra, Portugal 3 CFE – Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Apartado 3046, 3001-401 Coimbra, Portugal E-mail address: [email protected] (S. Ferreira). The halophyte plants Salicornia ramosissima and Scirpus maritimus are important phytoindicators and potential phytoremediators of trace metals, like cadmium. Their cadmium accumulation capacity and the influence of the salinity were tested in a greenhouse experiment, using Cd concentrations of 50 and 100 g l−1 and three salinities (0.0, 5.0 and 10.0). The Cd concentrations in their tissues were analyzed by Atomic Absorption Spectrometry, with Graphite Furnace Plant mortality, length and biomass increment were analyzed. For both species, mortality was accentuated at the highest salinities. Scirpus maritimus presented biomass increment, but S. ramosissima showed the opposite. The highest Cd accumulation was detected in roots and rhizomes, and diminished with the increase of salinity and Cd concentration. Although these plants survived well in brackish ecosystems, they showed limited trace metal phytoremediation at high salinity conditions, but are suitable for freshwater influenced environments. http://dx.doi.org/10.1016/j.copbio.2013.05.228 Enzyme and protein engineering beta-glucosidase from Pichia anomala MDD24 exhibiting transglycosylation property
Milk protein polymorphism in a Holstein–Friesian nucleus exploited in the North-East of Romania Teodor Bugeac 1 , Bâlteanu Valentin Adrian 2 , Creanga S¸teofil 1 , Ciobanu Marius Mihai 1 1
University Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad” din Ias¸i, Faculty of Animal Sciences, Romania 2 University of Agricultural Sciences and Veterinary Medicine, ClujNapoca – 400372, Faculty of Animal Sciences, Romania E-mail address: teodor [email protected] (T. Bugeac). Dairy farmers are nowadays adopting the molecular profiling in order to get some useful information for accelerating the selection process. Knowing the genotypes of dairy cattle and the positive associations between the genetic variants and production traits, helps the farmers to keep in their batch only the valuable animals. The aim of the present study is to identify the genotypes and to calculate genotype and allele frequencies for a nucleus of Holstein–Friesian cows exploited in the North-East of Romania. The milk samples were analyzed by the isoelectric focusing technique (IEF). The ␣S1-CN BB genotype recorded the highest frequency (0.94), while the C allele recorded a low frequency (0.03). For -CN locus A1A2 to A2A2 genotypes recorded the highest frequencies (0.26 for both genotypes), while the -CN A2C and BB had very low frequencies (0.03 each). For the K-CN locus, three genotypes were observed: AA, AB and BB. K-CN AB genotype recorded the highest frequency (0.69). As for the whey proteins, at -LG locus we identified three genotypes (AA, AB and BB), the highest frequency being calculated for -LG AB (0.44). The Holstein–Friesian cows took in study has some important alleles for milk production improvement, but especially for the milk quality: ␣S1-CN C; -CN A2; K-CN B and -LG B. The information can be used for milk improvement, especially of the casein content. For example, the cheese production capacity is associated with a higher content of protein, casein and fats. http://dx.doi.org/10.1016/j.copbio.2013.05.231
Kanit Vichitphan, Sukanda Vichitphan
Screening of novel isolated yeast strains for enhanced lipase production
Department of Biotechnology, Faculty of Technology; Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen 40002, Thailand E-mail address: [email protected] (K. Vichitphan).
Alexandra Ghiorghita 1,2 , Gheorghe Campeanu 2 , Misu Moscovici 2 , Florentina Israel Roming 1 , Angela Casarica 1,2 , Vasilica Manea 1,2 , Calina Petruta Cornea 1 1
Extracellular beta-glucosidase produced from Pichia anomala MDD24 was partially purified to characterize its transglycosylation property. beta-Glucosidase activities on p-nitrophenyl-beta-dglucopyranoside with various concentrations (0, 5, 10, 15, 20, 25 and 30%, V/V) of alcohols which were methanol, ethanol, 1propanol, 2-propanol and butanol were determined. The results showed that in the presence of 10% methanol, 15% ethanol, 10% 1-propanol, 15% 2-propanol and 5% buthanol, the beta-glucosidase activities were increased from 100% (0% alcohols) to the maximum enzyme activities of 174, 175, 174, 155 and 229%, respectively. Five tranglycosylation products (alkyl glucosides) from those reaction mixtures were examined by thin layer chromatography (TLC) analysis. Beside the bands of hydrolysis products and substrate on TLC results, the additional bands should be detected as methyl gluco-
University of Agronomic Sciences and Veterinary Medicine of Bucharest/Center for Applied Biochemistry and Biotechnology, 59 M˘ar˘as¸ti Blvd., 011464, 1st District, Bucharest, Romania 2 National Institute for Chemical Pharmaceutical Research and Development ICCF Bucharest, 112 Calea Vitan str., 031299, 3rd District, Bucharest, Romania E-mail address: alexandra [email protected] (A. Ghiorghita). Lipolytic enzymes production has been receiving more and more scientific interest during the last decades, due to expanding markets and increasing need for such novel biocatalysts. There is a growing demand for enhanced lipase catalysts, due to their wide applications in oil industry, detergents and pharmaceutics. In order to obtain a new microbial enzyme, after the isolation from a specific environment of the desired microorganism, proper selection
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143
equilibrium characterization. The method of state functions constructing procedure basing on local state equations is implemented exploratory for this system. A larger study should be conducted for an extended aquatic system.
side, ethyl glucoside, 1-propanyl glucoside, 2-propanyl glucoside and butyl glucoside, respectively, because it was not detected in the reaction without alcohol. http://dx.doi.org/10.1016/j.copbio.2013.05.230
http://dx.doi.org/10.1016/j.copbio.2013.05.227 Salinity influence on cadmium phytoremediation capacity of the halophyte plants Salicornia ramosissima and Scirpus maritimus from coastal environments (Portugal) Marcia Santos 1 , Carmen Ferreira 3
Pedro 1 , Silvia
Gonc¸alves 2 , Susana
1
GIRM – Marine Resources Research Group, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, Campus 4, Santuário Na. Sra. dos Remédios, 2520-641 Peniche, Portugal 2 IMAR – Marine and Environmental Research Centre, Department of Life Sciences, Faculty of Sciences and Technology, University of Coimbra, Largo Marquês de Pombal, 3004-517 Coimbra, Portugal 3 CFE – Centre for Functional Ecology, Department of Life Sciences, University of Coimbra, Apartado 3046, 3001-401 Coimbra, Portugal E-mail address: [email protected] (S. Ferreira). The halophyte plants Salicornia ramosissima and Scirpus maritimus are important phytoindicators and potential phytoremediators of trace metals, like cadmium. Their cadmium accumulation capacity and the influence of the salinity were tested in a greenhouse experiment, using Cd concentrations of 50 and 100 g l−1 and three salinities (0.0, 5.0 and 10.0). The Cd concentrations in their tissues were analyzed by Atomic Absorption Spectrometry, with Graphite Furnace Plant mortality, length and biomass increment were analyzed. For both species, mortality was accentuated at the highest salinities. Scirpus maritimus presented biomass increment, but S. ramosissima showed the opposite. The highest Cd accumulation was detected in roots and rhizomes, and diminished with the increase of salinity and Cd concentration. Although these plants survived well in brackish ecosystems, they showed limited trace metal phytoremediation at high salinity conditions, but are suitable for freshwater influenced environments. http://dx.doi.org/10.1016/j.copbio.2013.05.228 Enzyme and protein engineering beta-glucosidase from Pichia anomala MDD24 exhibiting transglycosylation property
Milk protein polymorphism in a Holstein–Friesian nucleus exploited in the North-East of Romania Teodor Bugeac 1 , Bâlteanu Valentin Adrian 2 , Creanga S¸teofil 1 , Ciobanu Marius Mihai 1 1
University Agricultural Sciences and Veterinary Medicine “Ion Ionescu de la Brad” din Ias¸i, Faculty of Animal Sciences, Romania 2 University of Agricultural Sciences and Veterinary Medicine, ClujNapoca – 400372, Faculty of Animal Sciences, Romania E-mail address: teodor [email protected] (T. Bugeac). Dairy farmers are nowadays adopting the molecular profiling in order to get some useful information for accelerating the selection process. Knowing the genotypes of dairy cattle and the positive associations between the genetic variants and production traits, helps the farmers to keep in their batch only the valuable animals. The aim of the present study is to identify the genotypes and to calculate genotype and allele frequencies for a nucleus of Holstein–Friesian cows exploited in the North-East of Romania. The milk samples were analyzed by the isoelectric focusing technique (IEF). The ␣S1-CN BB genotype recorded the highest frequency (0.94), while the C allele recorded a low frequency (0.03). For -CN locus A1A2 to A2A2 genotypes recorded the highest frequencies (0.26 for both genotypes), while the -CN A2C and BB had very low frequencies (0.03 each). For the K-CN locus, three genotypes were observed: AA, AB and BB. K-CN AB genotype recorded the highest frequency (0.69). As for the whey proteins, at -LG locus we identified three genotypes (AA, AB and BB), the highest frequency being calculated for -LG AB (0.44). The Holstein–Friesian cows took in study has some important alleles for milk production improvement, but especially for the milk quality: ␣S1-CN C; -CN A2; K-CN B and -LG B. The information can be used for milk improvement, especially of the casein content. For example, the cheese production capacity is associated with a higher content of protein, casein and fats. http://dx.doi.org/10.1016/j.copbio.2013.05.231
Kanit Vichitphan, Sukanda Vichitphan
Screening of novel isolated yeast strains for enhanced lipase production
Department of Biotechnology, Faculty of Technology; Fermentation Research Center for Value Added Agricultural Products (FerVAAP), Khon Kaen University, Khon Kaen 40002, Thailand E-mail address: [email protected] (K. Vichitphan).
Alexandra Ghiorghita 1,2 , Gheorghe Campeanu 2 , Misu Moscovici 2 , Florentina Israel Roming 1 , Angela Casarica 1,2 , Vasilica Manea 1,2 , Calina Petruta Cornea 1 1
Extracellular beta-glucosidase produced from Pichia anomala MDD24 was partially purified to characterize its transglycosylation property. beta-Glucosidase activities on p-nitrophenyl-beta-dglucopyranoside with various concentrations (0, 5, 10, 15, 20, 25 and 30%, V/V) of alcohols which were methanol, ethanol, 1propanol, 2-propanol and butanol were determined. The results showed that in the presence of 10% methanol, 15% ethanol, 10% 1-propanol, 15% 2-propanol and 5% buthanol, the beta-glucosidase activities were increased from 100% (0% alcohols) to the maximum enzyme activities of 174, 175, 174, 155 and 229%, respectively. Five tranglycosylation products (alkyl glucosides) from those reaction mixtures were examined by thin layer chromatography (TLC) analysis. Beside the bands of hydrolysis products and substrate on TLC results, the additional bands should be detected as methyl gluco-
University of Agronomic Sciences and Veterinary Medicine of Bucharest/Center for Applied Biochemistry and Biotechnology, 59 M˘ar˘as¸ti Blvd., 011464, 1st District, Bucharest, Romania 2 National Institute for Chemical Pharmaceutical Research and Development ICCF Bucharest, 112 Calea Vitan str., 031299, 3rd District, Bucharest, Romania E-mail address: alexandra [email protected] (A. Ghiorghita). Lipolytic enzymes production has been receiving more and more scientific interest during the last decades, due to expanding markets and increasing need for such novel biocatalysts. There is a growing demand for enhanced lipase catalysts, due to their wide applications in oil industry, detergents and pharmaceutics. In order to obtain a new microbial enzyme, after the isolation from a specific environment of the desired microorganism, proper selection