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Salivary calculi morphology: SEM and mCT correlative observation
E-Poster Presentation was performed by a BIOREVO BZ-9000 fluorescence microscope (Keyence). Results: We previously reported that anti-microtubule agents induced abnormal Fucci fluorescence in mitotic cells, which was also detectable after KPU-300 treatment. Furthermore, the abnormal fraction was confirmed to be positive for phospho-histone H3 (Ser10), a marker of mitosis. When KPU-300 was combined with X-irradiation, a synergistic radiosensitization was observed only when irradiation was given more than 16 h after KPU-300 treatment at the doses ≥30 nM. Radiosensitization was not obtained when X-irradiation was given before KPU-300 treatment. Taken together, it was suggested that KPU-300-induced synchronization of cells in M-phase could be the mechanism of the synergistic radiosensitization. To validate the hypothesis, we collected mitotic cells by a flow cytometric exclusion of red (G1-phase) cells combined with the shake-off method. The results showed that comparable radiosensitization was observed by this study. Conclusion: We conclude that KPU-300 could be a potent radiosensitizer, but the timing should be carefully considered to obtain efficient sensitization. http://dx.doi.org/10.1016/j.ijom.2015.08.266 Extensive squamous cell carcinoma of the ear – reconstruction with supraclavicular artery flap M. Oliveira 1,∗ , I. Pinto 2 , M. Mesquita 3 , R. Cardoso 4 , C. Queirós 3 , J. Carreiro 3 , L. Coelho 2 , A. Ferreira 3 1
Centro Hospitalar Lisboa Central, Maxillofacial Department, Lisbon, Portugal 2 Hospital São João, Maxillofacial Surgery Unit of Department of Plastic, Reconstructive, Aesthetic Surgery, Maxillofacial Surgery and Burn Unit, Porto, Portugal 3 Hospital Universitário de Coimbra, Department of Maxillofacial Surgery, Coimbra, Portugal 4 Hospital Universitário de Coimbra, Department of Stomatology, Coimbra, Portugal Squamous cell carcinoma is a common non-melanoma skin cancer. Frequently elderly people present with extensive carcinomas since they delay seeking medical help. Surgery might be challenging and the prognosis is worse. We present a case of an old woman with an extensive basal cell carcinoma of the ear with parotid involvement. After resection, we reconstructed the defect with a supraclavicular artery flap. The use of this flap in head and neck oncology is discussed. http://dx.doi.org/10.1016/j.ijom.2015.08.267
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Salivary calculi morphology: SEM and mCT correlative observation M. Lopes 1 , P. Nolasco 2 , C. Coelho 1 , P.V. Coelho 1 , M.F.C. Pereira 3 , A.P. Alves de Matos 4 , P.A. Carvalho 2 , M. Oliveira 1,∗ 1
Centro Hospitalar de Lisboa Central, Service of Maxillofacial Surgery, Portugal 2 University of Lisbon, CeFEMA, Department of Chemistry engineering, Instituto Superior Técnico, Portugal 3 University of Lisbon, CEGPIST, Department of Civil engineering, Architecture and Georesources, Instituto Superior Técnico, Portugal 4 Centro Hospitalar de Lisboa Central, Hospital Curry Cabral, Department of Pathological Anatomy, Portugal Several theories have been put forward regarding the aetiology and pathogenesis of salivary calculi, although a comprehensive understanding of the nucleation and growth mechanisms involved is still missing. The mechanism in the genesis of sialoliths formation remains largely unknown, theories defending an initial organic nidus or an initial precipitation of minerals, with subsequent deposition of organic and inorganic layers, can be found in the literature.1,2 Nevertheless, it remains object of discussion the etiologic factors responsible for the formation of the first nidus or the initial precipitation, since infection, inflammation of the gland, viscous nature of the mucous secretions or naturally existing sialomicroliths have all have been implicated.2–4 Aiming at an exhaustive systematization of salivary calculi morphogenesis, their morphology has been studied by computed micro-tomography (mCT) and scanning electron microscopy (SEM). mCT allowed for a precise localization of the core and other morphological features within the calculus volume, while investigation of details at higher resolution could be achieved with the SEM. However, due to the friable nature of the sialoliths, handling during sample preparation resulted often in material loss.
References Siddiqui, S. J. (2002). Brit Dent J, 193, 89. Teymoortash, A., et al. (2003). Arch Oral Biol, 48, 233. Grases, F., Santiago, C., et al. (2003). Clin Chim Acta, 334, 131. Harrison, J. D. (2009). Otolaryng Clin N Am, 42, 927.
http://dx.doi.org/10.1016/j.ijom.2015.08.268 Molecular targeted therapy of oral squamous cell carcinoma targeting HSP90 T. Onda 1,∗ , K. Hayashi 1 , S. Sekikawa 1 , R. Ohta 1 , Y. Watabe 1 , S. Ogane 1 , N. Takano 2 , T. Shibahara 1,2 1
Tokyo Dental College, Department of Oral and Maxillofacial Surgery, Chiba, Japan 2 Tokyo Dental College, Oral Cancer Center, Ichikawa, Japan Background: Effective cancer therapeutics may be established if single protein that regulates numerous signaling pathways showing abnormalities in cancer cells can be inhibited. Common upstream search was conducted using KeyMolnet software for proteins with expression abnormalities identified from proteome analysis of oral squamous cell carcinoma (OSCC) cells. As a result, HSP90 was identified as a target regulating the functional maintenance and stability of many client proteins involved in the proliferation and survival of OSCC cells.
Centro Hospitalar Lisboa Central, Maxillofacial Department, Lisbon, Portugal 2 Hospital São João, Maxillofacial Surgery Unit of Department of Plastic, Reconstructive, Aesthetic Surgery, Maxillofacial Surgery and Burn Unit, Porto, Portugal 3 Hospital Universitário de Coimbra, Department of Maxillofacial Surgery, Coimbra, Portugal 4 Hospital Universitário de Coimbra, Department of Stomatology, Coimbra, Portugal Squamous cell carcinoma is a common non-melanoma skin cancer. Frequently elderly people present with extensive carcinomas since they delay seeking medical help. Surgery might be challenging and the prognosis is worse. We present a case of an old woman with an extensive basal cell carcinoma of the ear with parotid involvement. After resection, we reconstructed the defect with a supraclavicular artery flap. The use of this flap in head and neck oncology is discussed. http://dx.doi.org/10.1016/j.ijom.2015.08.267
e271
Salivary calculi morphology: SEM and mCT correlative observation M. Lopes 1 , P. Nolasco 2 , C. Coelho 1 , P.V. Coelho 1 , M.F.C. Pereira 3 , A.P. Alves de Matos 4 , P.A. Carvalho 2 , M. Oliveira 1,∗ 1
Centro Hospitalar de Lisboa Central, Service of Maxillofacial Surgery, Portugal 2 University of Lisbon, CeFEMA, Department of Chemistry engineering, Instituto Superior Técnico, Portugal 3 University of Lisbon, CEGPIST, Department of Civil engineering, Architecture and Georesources, Instituto Superior Técnico, Portugal 4 Centro Hospitalar de Lisboa Central, Hospital Curry Cabral, Department of Pathological Anatomy, Portugal Several theories have been put forward regarding the aetiology and pathogenesis of salivary calculi, although a comprehensive understanding of the nucleation and growth mechanisms involved is still missing. The mechanism in the genesis of sialoliths formation remains largely unknown, theories defending an initial organic nidus or an initial precipitation of minerals, with subsequent deposition of organic and inorganic layers, can be found in the literature.1,2 Nevertheless, it remains object of discussion the etiologic factors responsible for the formation of the first nidus or the initial precipitation, since infection, inflammation of the gland, viscous nature of the mucous secretions or naturally existing sialomicroliths have all have been implicated.2–4 Aiming at an exhaustive systematization of salivary calculi morphogenesis, their morphology has been studied by computed micro-tomography (mCT) and scanning electron microscopy (SEM). mCT allowed for a precise localization of the core and other morphological features within the calculus volume, while investigation of details at higher resolution could be achieved with the SEM. However, due to the friable nature of the sialoliths, handling during sample preparation resulted often in material loss.
References Siddiqui, S. J. (2002). Brit Dent J, 193, 89. Teymoortash, A., et al. (2003). Arch Oral Biol, 48, 233. Grases, F., Santiago, C., et al. (2003). Clin Chim Acta, 334, 131. Harrison, J. D. (2009). Otolaryng Clin N Am, 42, 927.
http://dx.doi.org/10.1016/j.ijom.2015.08.268 Molecular targeted therapy of oral squamous cell carcinoma targeting HSP90 T. Onda 1,∗ , K. Hayashi 1 , S. Sekikawa 1 , R. Ohta 1 , Y. Watabe 1 , S. Ogane 1 , N. Takano 2 , T. Shibahara 1,2 1
Tokyo Dental College, Department of Oral and Maxillofacial Surgery, Chiba, Japan 2 Tokyo Dental College, Oral Cancer Center, Ichikawa, Japan Background: Effective cancer therapeutics may be established if single protein that regulates numerous signaling pathways showing abnormalities in cancer cells can be inhibited. Common upstream search was conducted using KeyMolnet software for proteins with expression abnormalities identified from proteome analysis of oral squamous cell carcinoma (OSCC) cells. As a result, HSP90 was identified as a target regulating the functional maintenance and stability of many client proteins involved in the proliferation and survival of OSCC cells.