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Satellite Cells in Denervated Laryngeal Muscles
Research Posters
R585 Surgical Outcome and Patient Benefit Following Septoplasty Sunil H. Vyas, MBchB (presenter); Rajkumar Singh, OBE, FRCS Rhyl United Kingdom; Kilmarnock United Kingdom
Problem: 1. Surgical outcome and patient benefit in terms of not only symptom improvement but also over how long a period. 2. If any medical treatment post - operatively makes any difference to symptology. Methods: This was an analysis of 368 primary septoplasties for clinically diagnosed deviated nasal septums with associated symptomology, Majority (80%) were performed with a standard hemitransfixation technique and the rest (20%) were submucus resection. Patients were discharged with either betnesol or otrivine. Results: 95% had great benefit from surgery, 86% of these having improvement within 3 weeks. There was earlier and greater improvement with both betnesol and otrivine initially. Symptoms were assessed via : (1) A symptomology questionnaire covering a 6 week period. (2) Clinical assessment for review at out - patient clinic after 6 weeks. Conclusion: Grade of operator ( consultant Vs. trainee ) affected outcome, as well as the position and extent of the septal deviation. Overall surgery was of great benefit to patients. There was greater improvement with initial post operative treatment with betnesol or otrivine. However there was no stastically significant difference in symptoms between the 2 groups. Significance: Septoplasty is of great benefit for deviated nasal septums with associated symptoms. Post-operative treatment with betnesol or otrovine makes a greater improvement.
R586 Subglottic Structure and Dimensions and Their Relation to Body Size and Gestational Age Victor S. Campelo (presenter); Leila Freire, MD; Luciana M Nita, MD; Luiz Ubirajara, MD; Rui Imamura, MD; Domingos H Tsuji, MD Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil
Problem: a major frequencie of respiratory injuries is one of the biggest problems in perinatal medical care. With the increased use of intubation, the incidence of laringeal injury leading to stenosis has also increased. The principal constriction point in infant larynx is the midcricoid. This study seeks to provide detailed morfometric data on the anatomy of cricoid cartilage and its relation to growth and body caracteristics of infants range from 5 to 9 months of gestational age. Methods: we studied 19 larynxs of 17 stillborns and 2 newborns (dead in the frist day). The gestational age range from 5 to 9 months. We made cricoid measurements with a mm-graduated ruler.
Results: wheight was the variable most related to cricoid measurements. The cricoid lumen configuration was similar to eliptic form and it didn‘t change with gestational age. The mean cricoid interior area was 19,279,62 mm2 and it was related with weight and height. The cricoid growth was most pronounced at the out portion of the cartilage. Conclusion: The cricoid lumen configuration was eliptic with a mean area smaller than that endotracheal tubes avaiable. This lumen area was most influenced by weight and height. Significance: its a experimental study that indicates diferences in lumen area of the cricoid cartilage and endotracheal tubes available.
R587 Satellite Cells in Denervated Laryngeal Muscles Michael J. Shinners, MD (presenter); Linda K. McLoon, PhD; George S Goding, Jr, MD Minneapolis MN; Minneapolis MN; Minneapolis MN
Problem: A process of continuous myonuclear addition has been demonstrated in the uninjured extraocular muscles and laryngeal muscles of the rabbit that is not present in skeletal limb muscle. These findings support the distinct properties of laryngeal muscles relative to the more widely studied limb muscles. The current study investigates the myonuclear response of rabbit laryngeal muscle to denervation. Methods: The recurrent laryngeal nerve was transected unilaterally in adult white albino rabbits. On day 7 or day 30, the rabbits received intraperitoneal injections of bromodeoxyuridine (brdU) every 2 hours for 12 hours followed by a 24 hour brdU free period. The larynx was harvested and studied with double staining immunohistochemistry for brdU and dystrophin. Results: At one week, there was a significant increase in brdU positive myonuclei in the thyroarytenoid muscle bilaterally. At one month, the contralateral side had returned to near baseline while the surgical side remained elevated. There was also an increase in the expression of the neonatal myosin heavy chain isoform in association with increased brdU positive myonuclei when compared to controls. Conclusion: Surgical denervation appears to upregulate the processes of myofiber remodeling in the rabbit laryngeal musculature in addition to inducing expression of the neonatal myosin fiber type. Significance: Ongoing experiments aim to further characterize these processes. A better understanding of the injury paradigm and the intrinsic differences of the laryngeal muscles compared to limb muscles may aid in treating laryngeal disorders. Support: AAO-HNS Foundation Grant
POSTERS
P158
Otolaryngology– Head and Neck Surgery August 2005
R585 Surgical Outcome and Patient Benefit Following Septoplasty Sunil H. Vyas, MBchB (presenter); Rajkumar Singh, OBE, FRCS Rhyl United Kingdom; Kilmarnock United Kingdom
Problem: 1. Surgical outcome and patient benefit in terms of not only symptom improvement but also over how long a period. 2. If any medical treatment post - operatively makes any difference to symptology. Methods: This was an analysis of 368 primary septoplasties for clinically diagnosed deviated nasal septums with associated symptomology, Majority (80%) were performed with a standard hemitransfixation technique and the rest (20%) were submucus resection. Patients were discharged with either betnesol or otrivine. Results: 95% had great benefit from surgery, 86% of these having improvement within 3 weeks. There was earlier and greater improvement with both betnesol and otrivine initially. Symptoms were assessed via : (1) A symptomology questionnaire covering a 6 week period. (2) Clinical assessment for review at out - patient clinic after 6 weeks. Conclusion: Grade of operator ( consultant Vs. trainee ) affected outcome, as well as the position and extent of the septal deviation. Overall surgery was of great benefit to patients. There was greater improvement with initial post operative treatment with betnesol or otrivine. However there was no stastically significant difference in symptoms between the 2 groups. Significance: Septoplasty is of great benefit for deviated nasal septums with associated symptoms. Post-operative treatment with betnesol or otrovine makes a greater improvement.
R586 Subglottic Structure and Dimensions and Their Relation to Body Size and Gestational Age Victor S. Campelo (presenter); Leila Freire, MD; Luciana M Nita, MD; Luiz Ubirajara, MD; Rui Imamura, MD; Domingos H Tsuji, MD Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil; Sao Paulo Brazil
Problem: a major frequencie of respiratory injuries is one of the biggest problems in perinatal medical care. With the increased use of intubation, the incidence of laringeal injury leading to stenosis has also increased. The principal constriction point in infant larynx is the midcricoid. This study seeks to provide detailed morfometric data on the anatomy of cricoid cartilage and its relation to growth and body caracteristics of infants range from 5 to 9 months of gestational age. Methods: we studied 19 larynxs of 17 stillborns and 2 newborns (dead in the frist day). The gestational age range from 5 to 9 months. We made cricoid measurements with a mm-graduated ruler.
Results: wheight was the variable most related to cricoid measurements. The cricoid lumen configuration was similar to eliptic form and it didn‘t change with gestational age. The mean cricoid interior area was 19,279,62 mm2 and it was related with weight and height. The cricoid growth was most pronounced at the out portion of the cartilage. Conclusion: The cricoid lumen configuration was eliptic with a mean area smaller than that endotracheal tubes avaiable. This lumen area was most influenced by weight and height. Significance: its a experimental study that indicates diferences in lumen area of the cricoid cartilage and endotracheal tubes available.
R587 Satellite Cells in Denervated Laryngeal Muscles Michael J. Shinners, MD (presenter); Linda K. McLoon, PhD; George S Goding, Jr, MD Minneapolis MN; Minneapolis MN; Minneapolis MN
Problem: A process of continuous myonuclear addition has been demonstrated in the uninjured extraocular muscles and laryngeal muscles of the rabbit that is not present in skeletal limb muscle. These findings support the distinct properties of laryngeal muscles relative to the more widely studied limb muscles. The current study investigates the myonuclear response of rabbit laryngeal muscle to denervation. Methods: The recurrent laryngeal nerve was transected unilaterally in adult white albino rabbits. On day 7 or day 30, the rabbits received intraperitoneal injections of bromodeoxyuridine (brdU) every 2 hours for 12 hours followed by a 24 hour brdU free period. The larynx was harvested and studied with double staining immunohistochemistry for brdU and dystrophin. Results: At one week, there was a significant increase in brdU positive myonuclei in the thyroarytenoid muscle bilaterally. At one month, the contralateral side had returned to near baseline while the surgical side remained elevated. There was also an increase in the expression of the neonatal myosin heavy chain isoform in association with increased brdU positive myonuclei when compared to controls. Conclusion: Surgical denervation appears to upregulate the processes of myofiber remodeling in the rabbit laryngeal musculature in addition to inducing expression of the neonatal myosin fiber type. Significance: Ongoing experiments aim to further characterize these processes. A better understanding of the injury paradigm and the intrinsic differences of the laryngeal muscles compared to limb muscles may aid in treating laryngeal disorders. Support: AAO-HNS Foundation Grant
POSTERS
P158
Otolaryngology– Head and Neck Surgery August 2005