Scientific Program

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

1

Scientific Program Saturday, July 6, 2002 Welcome

Program / Saturday, July 6

08:00 - 08:15

Nicholas Dainiak, Meeting Chair - Bridgeport, U.S.A.

08:15 - 09:00

1 Metcalf Lecture Chairs: Esmail D. Zanjani (Reno, NV, U.S.A.), Peter J. Quesenberry (Providence, RI, U.S.A.)

1

09:00 - 12:00

Hematopoietic Stem Cell Transplantation: Yesterday, Today and Tomorrow * R. Storb - Seattle, U.S.A. PLENARY SESSION

2 Stem Cell Differentiation and Apoptosis Chairs: Paul J. Simmons (Melbourne, Victoria, Australia), Hideaki Mizoguchi (Tokyo, Japan) 2

Transcriptional Programming in Haematopoietic Stem Cells * T. Enver, J. Brown, L. Bruno, K. Gale, R. Gupta, C. Heyworth, R. Hoffmann, C. Joshi, G. May, F. McBlane, S. Pearson, S. Sarno - London, United Kingdom

3

PU.1, A Transcriptional Regulator of Myelo- and Lymphopoiesis * H. Singh - Chicago, U.S.A.

4

Ikaros, Chromatin Regulation, and Hemopoietic Lineage Decisions * K. Georgopoulos - Charlestown, U.S.A.

5

DNA fragmentation during apoptosis and erythropoiesis * S. Nagata - Suita, Japan

6

Differential Gene Expression in Homogeneously Pure Stem Cells and Downstream Progeny * N. Iscove, P. Benveniste, M. Barbara, D. Hyam, M. Gu, N. Winegarden - Toronto, Canada

13:30 - 15:00

SIMULTANEOUS SESSION

MARQUETTE

3 Stem Cell Biology I Chairs: Gerald J. Spangrude (Salt Lake City, UT, U.S.A.), Norman N. Iscove (Toronto, ON, Canada) 7

Developmental potential of single Lin(-)Thy1.1(lo)c-kit(+)Sca-1(+) hematopoietic stem cells * A. Wagers, J. Christensen, R. Sherwood, I. Weissman - Stanford, U.S.A.

8

Trp53 null allele selectively expands hematopoietic progenitor and stem cells * J. Chen, M. Tekippe, C. Astle, D. Harrison - Bethesda, U.S.A.

9

Unexpected Cytokine Interactions Involved in the Stimulation of Hematopoietic Stem Cell and Progenitor Expansion in vitro * J. Piret, J. Audet, C. Eaves - Vancouver, Canada

10

Cobblestone area forming cells (CAFC) in cord blood do not represent LTC-IC * P. Denning-Kendall, S. Singha, J. Hows, Bristol - United Kingdom

11

Critical roles for the transcriptional repressors HES1 and HES5 during hematopoietic development. * S. Fraser, J. Hatakeyama, S. Mak Siu-Shan, M. Osawa, M. Ogawa, S.-I. Nishikawa, R. Kageyama - Kyoto, Japan

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Program / Saturday, July 6

Saturday, July 6, 2002 12

Hematopoietic Stem Cell homing Provides Clues to Epithelial Tissue Residence In Syngeneic and Allogeneic Hosts * M. Collector, S. Tyszko, S. Sharkis - Baltimore, U.S.A.

13

Age-related differences of retrovirally infected hematopoietic stem cells fate in mice reconstituted by fetal liver and bone marrow N. Drize, *M. Ershler, I. Nifontova, J. Chertkov - Moscow, Russian Federation

14

Primitive function of individual human hematopoietic progenitors is distributed assymetrically during the initial cell division T. Zhang, V. Eckstein, A. Ho, *M. Punzel - Heidelberg, Germany

13:30 - 15:00

SIMULTANEOUS SESSION

JOLLIET

4 Cytokines and their Receptors I Chairs: Nicholas Dainiak (Bridgeport, CT, U.S.A.), Grover C. Bagby (Portland, OR, U.S.A.) 15

Mice double deficient in Flt3 ligand and IL-7 receptor expression fail to develop B cells and lymphoid tissues * E. Sitnicka, C. Brakebusch, J. Peschon, S. Jacobsen - Lund, Sweden

16

Graded levels of IL-7R expression in hematopoietic stem cells influence myeloid versus B cell developmental potential in vivo * S. Purohit, C. Klug, B. Herrin, W. Leonard - Birmingham, U.S.A.

17

Dynamic of CAFC frequency in long-term bone marrow culture of TNF deficient mice * I. Nifontova, M. Ershler, N. Drize - Moscow, Russian Federation

18

Normal endogenous hematopoiesis in mice with a complete block in TGF-beta signalling. * J. Larsson, U. Blank, X. Fan, P. Leveen, S. Karlsson - Lund, Sweden

19

High correlation between clonality and thrombopoietin (TPO) hypersensitivity of megakaryocytic progenitor cells reveals a homogeneous population among patients diagnosed with essential thrombocythemia (ET) * A. Axelrad, D. Eskinazi, P. Correa, D. Amato, E. Liu, J. Prchal - Toronto, Canada

21

The Pharmacokinetics of Erythropoietin in the Cerebrospinal Fluid after Intravenous Administration of Recombinant Human Erythropoietin. * A. Xenocostas, W. Cheung, C. Zakszewski, M. Kelly, A. Lutynski, M. Crump, J. Lipton, T. Kiss, H. Messner - Toronto, Canada

22

Update on Mutations of the G-CSF Receptor Gene in Patients with Severe Congenital Neutropenia (CN) * C. Zeidler, K. Welte, M. Germeshausen - Hannover, Germany

213

Suppression of c-kit and the Epo-receptor in leukemic cell lines undergoing apoptosis through the activity of the COX-2 inhibitor celecoxib * Y. Ben-David, A. Koki, A. Truong, J. Lee, N. Sukai - Toronto, Canada

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

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Scientific Program Saturday, July 6, 2002 SIMULTANEOUS SESSION

DULUTH

5 Erythropoiesis Chairs: Kenneth S. Zuckerman (Tampa, FL, U.S.A.), Maurice C Bondurant (Nashville, TN, U.S.A.) 23

Ex Vivo and In Vivo Primitive and Definitive Hematopoiesis from a Non-Hematopoietic Stem Cell * M. Reyes, C. Verfaillie, L. Koodie, T. Lund, T. Lenvik, B. Jahagirdar - Minneapolis, U.S.A.

24

SCL rescues erythroid cell fate in the absence of EpoR signaling * R. Lahlil, E. Lecuyer, R. Martin, G. Krosl, T. Hoang, Montreal - Canada

25

FKHRL1 inhibits the cell cycle progression of erythroid cells viaup-regulation of p27/Kip1 * M. Uchida, T. Watanabe, K. Kirito, A. Iwama, M. Osawa, K. Ozawa, N. Komatsu - Kawachi-gun, Japan

26

Studies on Erythropoietin-dependent and Constitutive Tumor Necrosis Factor-alpha Expression in Murine and Human Erythroid Cells S. Jacobs-Helber, J.-C. Chen,, J. Ryan, E. Dessypris, A. Wickrema, M. Bondurant, * S. Sawyer - Richmond, U.S.A.

27

Transferrin Receptor 2 Inhibits Heme Synthesis in Erythroid Cells * S. Kim, H.-S. Yoon, P. Ponka - Montreal, Canada

28

Oxygen Sensing And Chuvash Polycythemia * S. Ang, D. Stockton, K. Hirota, V. Gordeuk, J. Jelinek, A. Sergueeva, P. Maxwell, G. Semenza, J. Prchal - Houston, U.S.A.

98

Human Prostate Epithelial Cells and Prostate Cancer Cells Express Functional Erythropoietin Receptors and Respond to Erythropoietin In Vitro * L. Feldman, Y. Wang, J. Rhim, A. Sytkowski - Boston, U.S.A.

30

Upregulation of HIF-1 pathway in congenital polycythemia with elevated Epo. * Y. Pastore, J. Jelinek, S. Ang, Y. Guan, E. Liu, K. Jedlickova, J. Prchal - Houston, U.S.A.

13:30 - 15:00

SIMULTANEOUS SESSION

MACKENZIE

6 Progenitor Mobilization and Expansion Chairs: Joao L. Ascensao (Reno, NV, U.S.A.), Ian K. McNiece (Denver, CO, U.S.A.) 31

Reversibility of CD34 Expression on Human Hematopoietic Stem Cells that Retain the Capacity for Secondary Reconstitution M. Dao, J. Arevalo, * J. Nolta - St. Louis, U.S.A.

32

Ex Vivo Expansion of Cord Blood CD34+ Cells on Human Cord Vein Endothelial Cells Derived from a Single Umbilical Cord R. J. Su, X. B. Zhang, * K. Li, Shatin - China

33

Membrane-separated cocultivation of hematopoietic stem and progenitor cells with stromal cell lines * T. Fischbach, T. Noll, Juelich - Germany

34

AMD-3100, an Antagonist of CXCR4, is a Potent Myeloid Progenitor Cell Mobilizing Agent in Mouse Strains Differing in Response to G-CSF-Induced Mobilization, and Synergizes with G-CSF in these Mice to Enhance Mobilization * H. Broxmeyer, S. Cooper, G. Hangoc, G. Bridger, Indianapolis -, U.S.A.

35

Selective elimination of immunoreactive T cells using a unique strategy to target P-glycoprotein * D. C. Roy, M. Guimond, A. Balassy, M. Barrette, S. Brochu, C. Perreault - Montreal, Canada

Program / Saturday, July 6

13:30 - 15:00

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Program / Saturday, July 6

Saturday, July 6, 2002 36

Analysis of mice congenic for loci influencing hematopoietic progenitor cell mobilization * H. Geiger, S. Szilvassy, P. Ragland, J. True, G. Van Zant - Lexington, U.S.A.

37

Levels of MMP-9 but not SDF-1 after G-CSF Mobilization are Correlated with Mobilizing Capacity J. Domenech, A. Carion, L. Benboubker, O. Herault, F. Roingeard, M. Degenne, G. Cartron, I. Desbois, P. Colombat, C. Binet - Tours, France

38

Ex vivo expansion of NKT cells from human cord blood CD34+ cells Y.-J. Jung, K.-H. Ryu, J.-H. Kie, W.-S. Chung, * J.-Y. Seoh - Yangchon-Ku, Korea

15:30 - 16:30

SIMULTANEOUS SESSION

MARQUETTE

7 Stem Cell Biology II Chairs: Connie J. Eaves (Vancouver, BC, Canada), Thalia Papayannopoulou (Seattle, WA, U.S.A.) 39

Hematopoietic Stem Cells Regenerate Myocardium in Ischemia-Induced Heart Disease: An Experimental Model * D. Orlic, A. Cline, R. Clevenger, S. Chimenti, R. Hoyt, P. Anversa, D. Bodine - Bethesda, U.S.A.

40

Lineage depleted bone marrow cells bind to cardiac myocyte cell line in vitro * A. Mihaliak, B. Hyett, H. Valinski, P. Becker - Worcester, U.S.A.

41

Comparison of the in vivo differentiation potential of neural and hematopoietic stem cells * F. Harder, N. Kirchhof, S. Petrovic, A. Müller - Wuertzburg, Germany

42

Muscle-derived hematopoietic stem cells are CD45 positive muscle SP that are derived from bone marrow SP * S. McKinney-Freeman, K. Jackson, S. Majka, K. Hirschi, M. Goodell - Houston, U.S.A.

43

Trafficking and Transdifferentiation of Bone Marrow Cells in a Skin Injury Model * M. Abedi, E. Badiavas, J. Lambert, G. Colvin, V. Falanga, P. Quesenberry - Providence, U.S.A.

15:30 - 16:30

SIMULTANEOUS SESSION

JOLLIET

8 Cytokines and their Receptors II Chairs: Camille N Abboud (Rochester, NY, U.S.A.), Thomas J. MacVittie (Baltimore, MD, U.S.A.) 44

Long-term repopulating ability of hematopoietic stem cells requires functional expression of Fibroblast Growth Factor-receptors * G. de Haan, E. Weersing, B. Dontje, L. Bystrykh, E. Vellenga, G. Miller, - Groningen, The Netherlands

45

The role of VEGF in hypoxic induction of hematopoietic progenitors from differentiating embryonic stem cells * S. Dang, P. Zandstra - Toronto, Canada

46

Vascular endothelial growth factor and thrombopoietin in myeloid disorders * R. Cacciola, E. Di Francesco, F. Stagno, R. Giustolisi, E. Cacciola - Catania, Italy

47

Endothelial cell transplantation rescues host hematopoiesis in lethally irradiated recipients B. Li, B. Liu, A. Bailey, *W. Fleming - Portland, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

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Scientific Program Saturday, July 6, 2002 SIMULTANEOUS SESSION

DULUTH

9 Apoptosis Chairs: Shigekazu Nagata (Suita, Osaka, Japan), Joseph Albanese (Kalamazoo, MI, U.S.A.) 48

Identification of two new FLIP-independent mechanisms for resistance to CD95-mediated apoptosis in lymphoma B cells * A. Lajmanovich, M. Irisarri, J. Molens, D. Leroux, J. Plumas - La Tronche, France

49

Role of N-terminal Cleavage of Bax in Regulating Apoptosis * X. Cao, X. Deng, W. May, Gainesville - U.S.A.

50

Survivin expression in granuloblastic cells from chronic myeloid leukemia * F. Stagno, E. Conte, P. Guglielmo, A. Santonocito, G. Giustolisi, U. Consoli, A. Messina - Catania, Italy

51

Stat5B unlike Stat5A plays a pivotal role in effecting hematopoietic recovery induced by Mpl-Ligand in lethally myelosuppressed mice * T. Pestina, C. Jackson - Memphis, U.S.A.

52

Altertions of C-myc Expression by Antisense Oligodeoxynucleotides Inhibite the Apoptosis Induced by Homoharringtonine In K562 Cells * W. Mai, M. Lin - Hangzhou, China

15:30 - 16:30

SIMULTANEOUS SESSION

MACKENZIE

10 Cell Cycle Control Chairs: G. David Roodman (Pittsburgh, PA, U.S.A.), Gerald B. Price (Montreal, QC, Canada) 53

Disruption of telomerase activity in human T lymphocytes leads to telomere dysfunction * A. Roeth, M. Schertzer, P. Lansdorp - Vancouver, Canada

54

Fanconi Anemia Type C and p53 Cooperate in Cell Cycle Control, Apoptosis and Tumorigenesis * B. Freie, S. Ciccone, X. Li, P. Plett, C. Orschell-Traycoff, H. Hannenberg, H. Broxmeyer, S.-H. Lee, D. Clapp - Indianapolis, U.S.A.

55 57 78

16:30 - 18:00

Withdrawn Expression of the hematopoietic stem cell marker CD34 is related to the cell cycle status S. Dougherty, *D. Pluznik - Bethesda, U.S.A. Expression of the Inhibitor of Apoptosis Protein, Survivin, is Up Regulated in Quiescent Cord Blood CD34+ Cells Before Entry into Cell Cycle * S. Fukuda, H.-m. Bian, L. Pelus - Indianapolis, U.S.A. POSTER SESSION

11 Apoptosis 58

Evaluation of GM-CSF Effect on Rate of Apoptosis in HL60 Cell line Undergoing Treatment by Cytotoxic Drugs and ATRA * R. Saadatian, A. Pourfatholah, M. Soleimani, F. Zaker - Tehran, Iran

59

The Benzene Metabolite Hydroquinone Sensitizes Bone Marrow Progenitor Cells to Tumor Necrosis Factor-alphainduced Apoptosis by Inhibiting NF-kB Activation * P. Kerzic, D. Pyatt, A. Le, S. Gross, R. Irons - Denver, U.S.A.

Program / Saturday, July 6

15:30 - 16:30

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Program / Saturday, July 6

Saturday, July 6, 2002 60

61 62 63

Induction of high surface CD95 Ligand (CD95L) expression on longterm stimulated human peripheral blood lymphocytes (PBL) * E.-M. Jacobsen, M. Ege, K.-M. Debatin, W. Friedrich, S. Mueller - Ulm, Germany Role of NF-kB in Regulation of Apoptosis of Erythroid Progenitor Cells N. Sae-ung, *K. Muta, Y. Abe, H. Nawata, S. Fucharoen - Fukuoka, Japan EDR, a pro- and anti-apoptotic molecule inducing erythroid differentiation * P. Dormer, E. Glas, E. Spitzer - Gauting, Germany The importance of mitchondrial localization for the prevention of irradiation-induced apoptosis by manganese superoxide dismutase J. Greenberger, J. Gretton, M. Jefferson, M. Bernarding, *M. Epperly - Pittsburgh, U.S.A.

64

Flow cytometry analysis of blocked proliferation in GM-progenitors * H. Sovalat, E. Wunder, C. Zanetti, P. Henon - Mulhouse, France

65

IL-12 plus IL-18 control peripheral histamine production by splenic basophil precursors through Fas-dependent apoptosis * E. Schneider, M.-B. Tonanny, M. Leite-De Moraies, M. Dy - Paris, France

66

16:30 - 18:00

Thrombopoietin-induced apoptosis during ex vivo expansion of human cord blood CD34+ cells G. Jang, H. Jin, J. Seoh, S. Im, K. Ryu, *H.-Y. Park - Seoul, Korea POSTER SESSION

12 Bone Marrow Stroma and Marrow Failure 67

Simultaneous Regeneration of Bone Marrow Stroma and Hematopoiesis: Role of Vascular Endothelial Growth Factor and Flt-3 V. Barak, *Z. Ben-Ishay - Jerusalem, Israel

68

Hematopoietic supportive stromal cell line was established from suspension fraction of long-term bone marrow culture * I. Nifontova, M. Ershler, Y. Olshanskaya, J. Chertkov, N. Drize - Moscow, Russian Federation

69

Long term co-culture of bone marrow cells with tumor cell line P388D1 M. Salimian, Z. Soheili, *B. Goliaei - Tehran, Iran

70

Influence of Bone Marrow Mesenchymal Cells on Stem/Progenitor Cell Expansion and its Modulation by Temperature E. Duthoit, *P. Bourin, J. Lataillade, L. Caillot - Clamart, France

71

72

73 74

The expression of vascular endothelial growth factor (VEGF) in aplastic anemia: relationship with microvessel density and bone marrow cellularity * J.-Y. Kwak, M. Chung*, H. Lim, S. Choi, C. Yim - Chonju, Korea The SDF-1/CXCR4 pathway is involved in the homing of chronic lymphocytic leukemia B cells and in their survival after migration into bone marrow stromal layers: inhibition by the CXCR4 antagonist (AMD3100) L. Lagneaux, A. Delforge, M. Dejeneffe, D. Schols, *D. Bron - Brussels, Belgium In vivo amplification of mouse mesenchymal precursor cells * N. Brouard, R. Driessen, B. Short, P. Simmons - Melbourne, Australia In vitro proliferation and expansion of hematopoietic progenitors from patients with bone marrow failure E. Flores-Figueroa, G. Martinez-Jaramillo, *H. Mayani - Mexico City, Mexico

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

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Scientific Program Saturday, July 6, 2002 POSTER SESSION

13 Cellular Interactions and Cell Cycle 75

In vitro production and analysis of dendritic cells which stimulate B lymphocyte immunity via production of exosomes * H. O'Neill, B. Quah, H. Wilson, K. Ni - Canberra, Australia

76

Determination of Platelet Activation Markers in post and pre-menopausal women by Flow cytometer * T. Roshan, J. Normah - Kota Bharu, Malaysia

77

Targeting Pancreatic Cancer with Armed Activated T cells directed at Her2/neu receptors * L. Lum, P. Davol, R. Grabert, N. Kouttab, A. Frackelton, R. Rathore - Providence, U.S.A.

79

Accessory cell interference in CFU-GM formation * E. Wunder, H. Sovalat, C. Zanetti, P. Henon - Mulhouse, France

80

Depletion of blood dendritic cells in patients with chronic myeloid leukemia correlates with CD34+/CD38primitive hematopoietic progenitors loss * M. Mohty, D. Blaise, N. Vey, D. Isnardon, D. Olive, B. Gaugler - Marseille, France

56

Hematopoietic surprises from the analysis of congenic mouse strains * Y. Liang, H. Geiger, G. Van Zant, Lexington - U.S.A.

16:30 - 18:00

POSTER SESSION

14 Cord Blood - Experimental and Clinical 81

The kinetics of immune reconstitution after cord blood transplantation in children; comparison with bone marrow transplantation and selected CD34+ stem cell transplantation * S. Kato, Y. Yasuda, Y. Inoue, H. Yabe, M. Sako - Isehara, Japan

82

Cryopreservation of umbilical cord blood without a rate-controlled program freezer supplies sufficient quality of stem cells for cord blood banking: a result of validation * S. Tsuchiya, M. Minegishi, T. Itoh, J. Fushimi, N. Miyagi - Sendai, Japan

83

Characterisation of Umbilical Cord Blood Lineage Negative Stem Cells producing Hemopoietic and Neuroglial Progenitors in vitro * N. Forraz, R. Pettengell, C. McGuckin, London - United Kingdom

84

Significant downregulation of inflammatory/immunoregulatory genes from LPS-activated cord blood (CB) versus adult peripheral blood (APB) monocytes (AMo) by oligonucleotide microarray gene expression profiling: implications for HLA disparate unrelated cord blood transplantation (UCBT) * H. Jiang, M. Jiang, L. Baxi, C. van de Ven, M. Cairo - New York, U.S.A.

85

Expression of myogenic markers in human umbilical cord blood * L. Lazzari, T. Montemurro, A. Giorgetti, P. Bianchi, E. Fermo, M. Belicchi, N. Bresolin, A. Zanella, P. Rebulla - Milan, Italy

86

Analysis of the kinetic output of hematopoietic stem cells in the collection of umbilical cord blood V. Adami, L. Dorotea, E. Falasca, L. Marini, P. Sala, F. Biffoni, G. Del Frate, C. Pipan, *A. Degrassi - Udine, Italy

87

Optimization of UCB ex-vivo expansion conditions G. Astori, W. Malangone, A. Risso, L. Bigi, P. Sala, F. Biffoni, G. Del Frate, C. Pipan, *A. Degrassi - Udine, Italy

88

Ang-2 mRNA in human CD34+ cord blood mononuclear cells J. Pomyje, J. Zivny, M. Plasilova, *E. Necas - Prague, Czech Republic

Program / Saturday, July 6

16:30 - 18:00

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Program / Saturday, July 6

Saturday, July 6, 2002 89

CD45 tyrosine phosphatase inhibits erythroid differentiation of umbilical cord blood CD34+ cells * A. Harashima, M. Suzuki, Y. Matsuo, T. Yoshioka, K. Orita - Okayama, Japan

90

Functional maturation of myeloid cells during ex vivo expansion of human cord blood CD34+ cells * J.-H. Kie, Y.-J. Jung, K.-H. Ryu, J.-Y. Seoh - Yangchon-Gu, Korea

16:30 - 18:00

POSTER SESSION

15 Cytokines/Growth Factors/Receptors 91 92

Regulation of Human Erythropoiesis by Activin A, BMP2 and BMP4, Members of the TGFβ Family * V. Maguer-Satta, L. Bartholin, M. Ffrench, R. Rimokh - Lyon, France A new unexpected role of the third complement component (C3a)-C3a receptor (C3aR) axis in regulating human hematopoiesis R. Reca, M. Majka, A. Janowska-Wieczorek, J. Lambris, *M. Ratajczak - Louisville, U.S.A.

93

Modulation of adhesion receptor and CXCR4 expression during cytokine culture of cord blood CD34+ cells * P. Denning-Kendall, S. Singha, J. Hows - Bristol, United Kingdom

94

Differential production and contribution to in vitro erythropoiesis of interleukin (IL)-11 and IL-8 in primary and secondary polyglobuly * S. Hermouet, A. Godard, D. Pineau, I. Corre, E. Lippert - Nantes, France

95

Angiogenic growth factors (VEGF, TGF-b1, bFGF) and thalidomide effect in myelofibrosis with myeloid metaplasia M. Biscardi, S. Gavazzi, F. Balestri, *A. Grossi - Florence, Italy

96

Development of lymphokine activated killer-like cells from cord blood cytotoxic T lymphocytes by 4-1BB co-stimulation * Y.-J. Kim, R. Brutkiewicz, H. Broxmeyer - Indianapolis, U.S.A.

97

Dominant Roles for Epo Receptor Y343 and STAT5 in proliferation and Kit Synergy * K. Li, C. Miller, S. Hegde, D. Barber, D. Wojchowski - State College, U.S.A.

29

Association Analysis of Individual Variability in the Erythropoietic Response to Hypoxia * K. Jedlickova, S. Witkowski, H. Chen, J. Stray-Gundersen, D. Stockton, B. Levine, J. Prchal - Houston, U.S.A.

99

Activation of STAT3 induces development of hematopoietic cells in the AGM region * K. Sekine, K. Minehata, M. Takeuchi, T. Sekiguchi, T. Yokota, A. Miyajima - Bunkyo-ku, Japan

100

Role of OncostatinM for the expansion of erythroid progenitors in bone marrow and spleen during the embryonic and neonatal stages * K.-I. Minehata, M. Takeuchi, K. Sekine, T. Hara, P. Donovan, Y. Hirabayashi, T. Inoue, A. Miyajima - Tokyo, Japan

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

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Scientific Program Saturday, July 6, 2002 POSTER SESSION

16 Erythropoiesis 101 102

Hypoxia-Stimulated Erythropoietin Production in Mice Under Treatments to Keep the Red Cell Mass from Rising * C. Bozzini, A. Barcelo, M. Martinez, M. Conti - Buenos Aires, Argentina Nitric Oxide Production by Bone Marrow in Response to Gamma Interferon: Association with the Anemia of Chronic Disease G. Dallalio, A. Van Laer, A. Gelasco, *R. Means Jr., - Charleston, U.S.A.

103

Human Erythroid Cell Stimulating Factor : Characterization and functional role in aging * S. Ponnappan, U. Ponnappan, K. Udupa - Little Rock, U.S.A.

104

Physiologic regulation of erythropoiesis determined by automated hematology analyzer * F.-s. Wang, K. Hirai, Y. Hamaguchi - Long Grove, U.S.A.

105

Role of Erythropoietin-Induced Erythropoiesis in the Course and Outcome of Malaria * K.-H. Chang, M. Stevenson - Montreal, Canada

106

In vitro Effects of p38 MAP Kinase Inhibitors on Proliferation and Differentiation of Murine Bone Marrow Stem Cells and Erythroid Progenitors * C. Zhang, D. McFarland, L. Hahn, D. Dalmas, T. Sellers, L. Tierney, H. Thomas, L. Schwartz, P. Narayanan - King Of Prussia, U.S.A.

107

Erythropoietin as modulator of cytokine-synthesizing activity of erythroid cells * V. Kozlov, T. Injelevskaya, S. Krysov, S. Sennikov - Novosibirsk, Russia

108

Effects of p38 MAP kinase inhibitors on the maturation and differentiation of murine erythroid progenitors cells * D. Dalmas, M. Scicchitano, C. Zhang, D. McFarland, L. Tierney, P. Naraynan, L. Schwartz - Norristown, U.S.A.

109

Large scale ex vivo production of immature human erythroid cells for transfusional purposes. T. Neildez-Nguyen, H. Wajcman, M. Marden, M. Bensidhoum, V. Moncollin, M. C. Giarratana, L. Kobari, D. Thierry, *L. Douay - Paris, France

110

Thrombopoietin Acts Synergistically with Leukemia Inhibitory Factor to Maintain an Undifferentiated State of Embryonic Stem Cells Homozygous for a Shp2 Deletion Mutation X. Xie, *R. Chan, M. Yoder, Indianapolis - U.S.A.

111

Reduction of Cell Cycle Progression in Human Erythroid Progenitor Cells Treated with Tumor Necrosis Factor Alpha (TNF) Occurs with Reduced CDK6 and is Reversed by CDK6 Transduction * C. Dai, I. Chung, S. Jiang, S. Krantz - Nashville, U.S.A.

112 113

Nuclear localization of porphobilinogen deaminase in erythroleukemic differentiation Y. Gozlan, D. Schwartz, D. Katcoff, *Z. Malik - Ramat Gan, Israel Macrophage-erythroblasts interactions within bone marrow erythroblastic islands in cyclophosphamide-treated rats after hypobaric hypoxia * V. Kalaydjieva, R. Mileva - Sofia, Bulgaria

Program / Saturday, July 6

16:30 - 18:00

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Program / Saturday, July 6

Saturday, July 6, 2002 16:30 - 18:00

POSTER SESSION

17 Gene Function and Therapy 114

A New Protocol Using an Engineered Lentiviral Vector Allows Maximal Transduction and Expression in Human Hematopoietic Primitive Cells and their Progeny S. Amsellem, E. Ravet, S. Fichelson, *F. Pflumio, A. Dubart-Kupperschmitt - Paris, France

115

Role of Dendritic Cells in TNF-alpha Gene Therapy of Leukemia With Hematopoietic Progenitor Cells * S. Gautam, Y. Xu, X. Gao, N. Janakiraman, R. Chapman - Detroit, U.S.A.

116

A fast and efficient gene transfer protocol for umbilical cord blood CD34+ cells with different pseudotyped retroviral vectors * H. Rozemuller, G. Kaptijn, A. Hagenbeek, A. Martens - Utrecht, The Netherlands

117

The “editorial” repair of the mutation sites in the DNA and the normal hemopoiesis correction * R. Lokhov - Vladikavkaz, Russian Federation

118

Effects of retroviral mediated gene transfer on homing of transduced human hematopoietic progenitor cells in NOD/SCID mice * K. Hall, K. Cornetta, R. Abonour, E. Srour - Indianapolis, U.S.A.

119

Differential patterns of transgene expression in human hematopoietic cells transduced with oncoretroviral and lentiviral vectors * G. Guenechea, J. C. Segovia, M. Ramírez, O. Gan, C. Dorrell, A. Limón, J. Barquinero, J. E. Dick, J. A. Bueren - Madrid, Spain

120

Engraftment into NOD/SCID mice of transduced hematopoietic stem cells from cord blood * S. Lucchi, L. Lazzari, S. Bruno, L. Porretti, W. Piacibello, P. Rebulla - Milan, Italy

121

Chemoprotection of marrow-repopulating human peripheral blood progenitor cells transduced with the human multidrug resistance-1 gene * S. Laufs, B. Schiedlmeier, A. Schilz, J. Topaly, K. Kühlcke, C. Baum, W. Zeller, H.-G. Eckert, S. Fruehauf - Heidelberg, Germany

122

Intramarrow human IL-1 alpha gene transfer into non-human primate after total body gamma irradiation * N. Becard, T. De Revel, J. Lefaix, T. Sorg, D. Dormont, R. Le Grand - Fontenay aux Roses, France

123

Feasibility of lentiviral vector based gene therapy strategies of AIDS : evaluation of transduction efficacy in bone marrow CD34+ cells purified from SIV infected cynomolgus macaques * W. Gay, D. Negre, F. Cosset, H. Thiebot, S. Derdouch Kielbowicz, E. Lauret, D. Dormont, R. Le Grand - Fontenay aux Roses, France

124

Hematopoietic Phenotype of Fanconi Anemia A Knockout Mice and Correction of the In Vitro Bone Marrow Growth Defects with Human FANCA-encoding Retroviral Vectors * P. Rio, J. Segovia, H. Hanenberg, J. Casado, J. Martinez, S. Lobitz, N. Cheng, H. Van de Vrugt, F. Arwert, H. Joenje, J. Bueren - Madrid, Spain

125

Lentiviral gene transfer and ex-vivo expansion of human primitive stem cells capable of multilineage repopulations (secondary and tertiary) in NOD/SCID mice * W. Piacibello, S. Bruno, F. Sanavio, L. Naldini, M. Aglietta - Candiolo, Italy

126

Hodgkin’s Reed-Sternberg Cell Lines Express a Intergenically Spliced mRNA Containing DEC-205 and a Novel C-Type Lectin DCL-1 * M. Kato, N. Gonzalez, B. Cooper, S. Khan, N. Angel, D. Hart - South Brisbane, Australia

127

MnSOD Gene Transfer To CD34+Thy-1+lin- Human Cord Blood Cells Using Herpes Simplex Virus Type 1 Vectors * J. Goff, D. Shields, J. Wechuck, D. Wolfe, S. Huang, K. Hanley, W. Goins, M. Epperly, J. Glorioso, J. Greenberger, Pittsburgh, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

11

Scientific Program Saturday, July 6, 2002 Optimization of naked DNA gene delivery protocols using dimeric form of erythropoeitin as a reporter * A. Vilalta, R. Mahajan, K. Tonsky, P. Hobart - San Diego, U.S.A.

129

Global Gene Expression Analysis of Human Hematopoietic Stem Cells * K. Takenaka, J. Dick - Toronto, Canada

130

Potentiation of Erythropoeitin by cytokine co-administration using naked DNA. * A. Vilalta, P. Hobart - San Diego, U.S.A.

131

Positive regulation of plasmid based gene expression in vivo using the tetracycline repressor (TetR) protein * M. Margalith, A. Vilalta, P. Hobart - San Diego, U.S.A.

132

Analysis of Radiation-Associated Changes in Gene Expression Using Microarray Technology * S. Schreyer, L. Karkanitsa, J. Albanese, N. Dainiak - Bridgeport and Kalamazoo, U.S.A.

Program / Saturday, July 6

128

12

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Sunday, July 7, 2002 08:00 - 09:30

PLENARY SESSION

18 Malignant Transformation and Targeted Therapy

Program / Sunday, July 7

Chairs: Grover C. Bagby (Portland, OR, U.S.A.), Ronald Hoffman (Chicago, IL, U.S.A.) 133

CML: A Targeted Therapy: Lessons Learned * B. Druker - Portland, U.S.A.

134

Deletions of the Derivative Chromosome 9 in CML – Double Jeopardy from a Single Translocation * A. Green - Cambridge, United Kingdom

135

Molecular Dissection of Hox-induced Leukemia * G. Sauvageau, J. Yao, E. Kroon - Montreal, Canada

136

Role of macrophage inflammatory protein-1-alpha in bone destruction and tumor burden in an in vivo model of human myeloma bone disease * G. D. Roodman - Pittsburgh, U.S.A.

10:00 - 12:00

SIMULTANEOUS SESSION

MARQUETTE

19 Leukemia - Experimental Chairs: Pierre Laneuville (Montreal, QC, Canada), John E. Dick (Toronto, ON, Canada) 137

Analysis of human leukemic cells following injection into pre-implantation murine blastocysts * M. Dürr, F. Harder, R. Henschler, A. Müller - Wuertzburg, Germany

138

Development of human leukemia in murine models is associated with de novo angiogenesis: Relevance for the design of novel anti-leukemia therapies * P. Veiga, S. Sallan, L. Nadler, A. Cardoso - Boston, U.S.A.

139

PML/RARalpha and FLT3/ITD induce an APL-like disease in a mouse model * L. Kelly, J. Kutok, I. Williams, S. Amaral, C. Boulton, D. Curley, T. Ley, D. Gilliand - Boston, U.S.A.

140

Expression of BCR/ABL in primary human CD34+ progenitors reproduces abnormalities in proliferation and adhesion seen in primary chronic myelogenous leukemia progenitors, which are reversed by a kinase inactivating mutation * R. Bhatia, P. Ramaraj, N. Niu, S. Chu, J. Yee - Duarte, U.S.A.

141

Effect of FKHRL1 on STI571-induced cell cycle arrest in chronic myelogenous leukemia * M. Mori, T. Watanebe, M. Uchida, K. Kirito, T. Nagai, T. Tauchi, K. Miyazawa, K. Ozawa, N. Komatsu - Minamikawachi-machi, Japan

142

In Vivo Efficacy of Diphtheria Toxin-Growth Factor Fusion Proteins Against Primitive Acute Myeloid Leukemia Progenitors is Enhanced by Co-administration of Cytosine Arabinoside * D. Hogge, M. Feuring-Buske, B. Gerhard, A. Frankel - Vancouver, Canada

143

Imatinib in combination with 17-allylamino-17-demethoxy-geldanamycin (17-AAG) for treatment of chronic myelogenous leukemia * J. Topaly, M. Schad, W. Zeller, S. Fruehauf - Heidelberg, Germany

144

The Nucleoporin NUP98-Topoisomerase I (NUP98-TOP1) fusion gene induces marked myelomonocytic proliferative abnormalities in murine hematopoietic cells in vitro and in vivo * R. Gurevich, N. Pineault, J. Antonchuk, P. Aplan, R. Humphries - Vancouver, Canada

145

Will drug combinations effectively eradicate quiescent leukaemic stem cells in Chronic Myeloid Leukaemia (CML)? * H. Jorgensen, E. Allan, S. Graham, L. Richmond, J. Godden, T. Holyoake - Glasgow, United Kingdom

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

13

Scientific Program Sunday, July 7, 2002 10:00 - 12:00

SIMULTANEOUS SESSION

JOLLIET

20 Cord Blood Transplantation Chairs: Philip B. McGlave (Minneapolis, MN, U.S.A.), Norbert-Claude Gorin (Paris, France) 146

147

Differentiation of cord blood CD34+ stem cell-derived dendritic cells in context of phentotype, structure and functions as a tool for immunotherapy S. Bykovskaia, W. Storkus, *J. Shogan - Pittsburgh, U.S.A.

148

In utero transplantation of human cord blood stem cells: a new approach by the intracelomic route in the fetal ovine model G. Noia, *G. Monego, L. Pierelli, A. Perillo, S. Mancuso - Rome, Italy

149

Ex vivo myeloid differentiation of cord blood CD34+ cells: comparison of 4 serum-free media containing bovine or human albumin C. De Bruyn, *A. Delforge, D. Bron - Bruxelles, Belgium

150

Flt-3 Ligand Enchanced Ex Vivo Expansion of Progenitor and NOD/SCID Repopulating Cells in a Preclinical System X. Zhang, R. J. Su, *K. Li - Shatin, China

151

Improved survival rates after transplantation of combined, low-dose placental cord blood (PCB) grafts in a mouse model * A. Scaradavou, D. Sung, P. Rubinstein - New York, U.S.A.

152

Reduced intensity (RI) allogeneic stem cell transplantation (AlloSCT) from related and unrelated donors in children and adolescents with malignant and non-malignant disease * M. Cairo, L. Harrison, K. Wolownik, C. Wischhover, G. Del Toro, D. George, B. Bradley - New York, U.S.A.

153

Comparative Outcome of Unrelated Cord Blood (CB) VS Bone Marrow (BM) Hematopoietic Stem Cell Transplantation (HSCT) in Children: Hôpital Sainte-Justine’s Experience * J.-H. Dalle, M. Duval, A. Moghrabi, E. Wagner, S. Barrette, J. Champagne, J. Demers, M. Vachon, M. Champagne Montreal, Canada

154

Detection of Minimal Residual Disease after Allogeneic Hematopoietic Transplantation Using the BioView (DuetTM) Combined Morphological and Cytogenetical analysis * A. Shimoni, A. Nagler, M. Reichart, C. Kaplinski, L. Trakhtenbrot - Tel Hashomen, Israel

10:00 - 12:00

SIMULTANEOUS SESSION

DULUTH

21 Cellular Interactions and Bone Marrow Stroma Chairs: Willem E. Fibbe (Leiden, The Netherlands), Kateri A. Moore (Princeton, NJ, U.S.A.) 155

Platelet- and tumor-cell-derived microparticles as new-identified regulators of tumor angiogenesis * M. Majka, J. Kijowski, A. Janowska-Wieczorek, M. Ratajczak - Louisville, U.S.A.

156

Alterations in integrin functional state modulate the response to adhesion-mediated growth inhibitory signals in ex vivo generated long-term culture-initiating cells * S. Huygen, R. Greimers, I. Kato, Y. Beguin, A. Gothot - Liege, Belgium

157

TNF-alpha modulates Jagged2 and Notch expression in the bone marrow microenvironment * N. Carlesso, A. Chora, E. Cruz, F. Cristina, B. Goumnerov, L. Rahme, J. Aster, A. Cardoso, D. Scadden - Charlestown, U.S.A.

Program / Sunday, July 7

In Vitro and In Vivo Differentiation of Megakaryocyte Progenitor Cells Derived from Cord Blood using a TPO Peptide Agonist or Thrombopoietin and Flt-3L, SCF, and VEGF * S. Smith, J. Reems, R. Xu, R. Moldwin, J. Kiss - Arlington Heights, U.S.A.

14

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program

Program / Sunday, July 7

Sunday, July 7, 2002 159

Anti-CD3 x Anti-HER2/neu Armed T cells Inhibit Tumor Development or Induced Remissions in Prostrate Cancer Bearing Beige/SCID Mice * J. Smith, P. Davol, N. Kouttab, R. Rathore, G. Elfenbein, L. Lum - Providence, U.S.A.

160

Molecular profiling of mouse mesenchymal precursor cells * N. Brouard, T. Occhiodoro, B. Short, P. Simmons - Melbourne, Australia

161

Effect of p38 Inhibitors on Cytokine Production in Human Bone Marrow Stromal Cells: A Comparison With Inhibitors of the NF-kB, Protein Kinase C and MEK1 Pathways * M. Scicchitano, S. Kokan, S. Byers, L. Tierney, L. Schwartz, D. McFarland - King of Prussia, U.S.A.

162

Factors in Granulopoiesis Produced by Bone Marrow Stromal Cells * M. Hiramoto, Y. Kawakami, D. Shima, H. Handa, S. Aizawa - Itabashi-Ku, Japan

163

Mesenchymal Stem Cells Promote Leukemia Engraftment in Murine Model of Primary Human Leukemia * J. Yu, L.-E. Shao, T. Tanaka, R. Gribi - La Jolla, U.S.A.

164

Pediatric Bone Marrow Mesenchymal Stem/Progenitor Cells Favour the Short Term Expansion of Primitive Overcommitted Progenitors * A. Tocci, L. Luchetti, D. Arduini, G. Isacchi - Rome, Italy

10:00 - 12:00

SIMULTANEOUS SESSION

MACKENZIE

22 Signal Transduction Chairs: Gerald Krystal (Vancouver, BC, Canada), Mervin C. Yoder (Indianapolis, IN, U.S.A.) 165

VEGF-A dosage determines the hematopoietic output during development * R. Martin, R. Lahlil, A. Damert, L. Miquerol, G. Keller, A. Nagy - Montreal, Canada

166

NF-KB negatively regulates erythropoietin expression in inflammation * K. La Ferla, T. Hellwig-Buergel, W. Jelkmann - Luebeck, Germany

167

Requirement of a Src family kinase activity for chemokine signaling in normal and malignant hematopoietic cells * A. Ptasznik, E. Urbanowska, S. Chinta, M. Stanislaus, A. Gewirtz - Philadelphia, U.S.A.

168

The SOCS box of suppressor of cytokine signalling 1 (SOCS1) is essential for full inhibition of cytokine signalling in vivo * W. Alexander, J. Zhang, D. Metcalf, N. Nicola, D. Hilton - Parkville, Australia

169

The Inositol Polyphosphate 4-Phosphatase B Is Down-Regulated In The Osteopetrotic Grey Lethal Mutant Mouse * M. Ferron, J. Vacher - Montréal, Canada

170

Investigation of the localization of the second messenger phosphatidylinositol-(3,4,5)-trisphosphate in chronic myeloid leukemia * D. Xenaki, A. Gray, C. Downes, P. Owen-Lynch - Lancaster, United Kingdom

171 172

Regulation of stroma cell-derived factor 1 (SDF-1) signaling in human hematopoietic progenitor cells A. Vichalkovski, K. Kirschner, K. Baltensperger, *H. Porzig - Bern, Switzerland MAP Kinase Is Activated Differentially Among Hematopoietic Cells * C. Chen, A. J. Sytkowski - Boston, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

15

Scientific Program Sunday, July 7, 2002 13:30 - 15:00

PLENARY SESSION

23 Signalling in Normal and Malignant Cells Chairs: James D. Griffin (Boston, MA, U.S.A.), R. Keith Humphries (Vancouver, BC, U.S.A.) Memory stem cells * A. Lanzavecchia - Bellinzona, Switzerland

174

Adoptive Immunotherapy with Th1, Th2 and T Regulatory T Cells * C. June - Philadelphia, U.S.A.

175

The Role of SHIP in Hemopoietic Cell Signalling * G. Krystal - Vancouver, Canada

176

Genetic interaction between SCL and E2A in lymphocyte development and in acute lymphoblastic leukemias * T. Hoang, S. Herblot, P. Aplan - Montreal, Canada

15:30 - 16:30

SIMULTANEOUS SESSION

MARQUETTE

24 Leukemia - Clinical Chairs: Peter J. Quesenberry (Providence, RI, U.S.A.), Denis-Claude Roy (Montreal, QC, Canada) 177

Rapid decline of BCR-ABL+ progenitors(CFC, LTC-IC) in the bone marrow of CML patients in chronic and accelerated phase on treatment with STI571 after failure or intolerance to interferon alpha * A. Petzer, E. Gunsilius, H. Duba, F. Elahi, E. Hoflehner, M. Haun, D. Wolf, J. Thaler, K. Geissler, K. Gruenewald, E. Woell, G. Gastl, F. Keil - Innsbruck, Austria

178

Telomere length in peripheral blood granulocytes reflects response to treatment with STI571 * T. Brummendorf, I. Ersoz, U. Hartmann, A. Wahl, S. Balabanov, S. Kreil, T. Lahaye, U. Berger, H. Gschaidmeier, C. Bokemeyer, P. Lansdorp, R. Hehlmann, L. Kanz, A. Hochhaus - Tübingen, Germany

179

Expression of Selectin Ligands on Blasts from Human Acute Leukemias * R. Sackstein, C. Dimitroff, J. Lee - Boston, U.S.A.

180

Global down regulation of HOX gene expression in Acute Promyelocytic Leukemia patients * A. Thompson, M. Quinn, D. Grimwade, T. Lappin - Belfast, United Kingdom

181

Doubling Time of Soluble CD23 is a Biological Marker for Disease Progression of Stage a B-CLL. * N. Meuleman, L. Lagneaux, M. Dejeneffe, J. Benani, T. Tondreau, M. Sarfati, D. Bron - Brussels, Belgium

15:30 - 16:30

SIMULTANEOUS SESSION

JOLLIET

25 Granulopoiesis and Thrombopoiesis Chairs: Fred R. Sullivan (Houston, TX, U.S.A), Edward F. Srour (Indianapolis, IN, U.S.A.) 182

The polybasic motif of the RhoGTPase Rac2, located in the C-terminus domain, is responsible for the specific function of Rac2 in superoxide production but not Chemotaxis * M. Filippi, A. Yamauchi, Y. Gu, A. Ayele, M. Dinauer, D. Williams - Cincinnati, U.S.A.

183

Fps/Fes and Fer protein tyrosine-kinases play redundant roles in regulating myelopoiesis * A. Craig, Y. Senis, P. Greer - Kingston, Canada

Program / Sunday, July 7

173

16

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program

Program / Sunday, July 7

Sunday, July 7, 2002 184

Idiopathic Hypereosinophilic Syndrome: in vivo response to Imatinib Mesylate, and inhibition of eosinophil progenitors in vitro * C. Abboud, A. Jankov, R. Felgar, A. Arbini, K. Rosell, J. Liesveld - Rochester, U.S.A.

185

Thrombopoietin, but not cytokines binding to gp130 protein-coupled receptors, activates MAPKp42/44, AKT and STAT proteins in normal human CD34+ cells, megakaryocytes and platelets * M. Majka, J. Ratajczak, A. Janowska-Wieczorek, M. Ratajczak - Louisville, U.S.A.

15:30 - 16:30

SIMULTANEOUS SESSION

DULUTH

26 Chemokines Chairs: Hal E Broxmeyer (Indianapolis, IN, U.S.A.), Suzanne Watt (Oxford, United Kingdom) 186

Direct Evidence that Stromal Derived Factor-1 Inhibits the Proliferation of Human Hemopoietic Stem Cells in NOD/SCID Mice * J. Cashman, I. Clark-Lewis, A. Eaves, C. Eaves - Vancouver, Canada

187

CD26/DPPIV Regulation of CXCL12/SDF-1α Mediated Hematopoietic Stem Cell Migration * K. Christopherson II, H. Broxmeyer - Indianapolis, U.S.A.

188

SDF-1 regulates primitive hematopoiesis by promoting G0/G1transition in CD34+ cells J. Lataillade, *P. Bourin, F. Herodin, M. Le Bousse-kerdiles - Clamart, France

189

CXCR4SDF-1 signaling is active in rhabdomyosarcoma cells and regulates locomotion, chemotaxis and adhesion. The role of PAX3-FKHR in expression of CXCR4 J. Libura, J. Drukala, A. Janowska-Wieczorek, F. Barr, *M. Ratajczak - Louisville, U.S.A.

190

15:30 - 16:30

Differential Expression Patterns of SDF-1 and MMP-9 by Human Bone Marrow Stromal and Endothelial Cells Before and After Stimulation by G- or GM-CSF * A. Carion, S. Iochman, P. Charbord, N. Clement, O. Herault, P. Reverdian, B. Weksler, C. Binet, J. Domenech - Tours, France SIMULTANEOUS SESSION

MACKENZIE

27 Radiation Effects Chairs: Gerard Wagemaker (Rotterdam, The Netherlands), Shirley Lehnert (Montreal, QC, Canada) 192

Plasma Flt3-ligand (FL) concentration as a bio-indicator of radiation-induced bone marrow aplasia * J. M. Bertho, J. Frick, C. Demarquay, A. Chapel, D. Thierry, J. Aigueperse - Fontenay aux Roses, France

193

Protection of pulmonary endothelial cells from irradiation by overexpression of manganese superoxide dismutase-plasmid/liposome complexes * J. Greenberger, H. Guo, J. Gretton, M. Jefferson - Pittsburgh, U.S.A.

194

The short term injection of antiapoptotic cytokine combinations to mice early after lethal gamma irradiation promotes survival * F. Herodin, P. Bourin, J. Lataillade, M. Drouet - La Tronche, France

195

Effect of Deep Space Radiation on Human Hematopoietic Stem Cell Function * M. Stanislaus, P. Bennett, P. Guida, G. Danet, J. Luongo, B. Sutherland, A. Gewirtz - Philadelphia, U.S.A.

196

Role of ionizing radiation in cell survival: A model for dissecting common pathways in the bone marrow microenvironment * J. Albanese, N. Dainiak - Kalamazoo, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

17

Scientific Program Sunday, July 7, 2002 16:30 - 18:00

POSTER SESSION

28 Graft vs Host Disease and Minimal Residual Disease 197

Detection of aneuploid CD38+ plasmacells and aneuploid CD19+ B-lymphocytes in bone marrow, peripheral blood and PBSC harvest of myeloma patients A. Santonocito, U. Consoli, *G. Milone, F. Indelicato, S. Leotta, G. Moschetti, P. Guglielmo, R. Giustolisi - Catania, Italy

199

T-cell receptor beta gene locus as a target for minimal residual disease detection in acute lymphoblastic leukemia * X. Chen, G. Neale - Memphis, U.S.A.

200

Ex-vivo exposure to fludarabine reduces allo-proliferation and preserves homeostatic proliferation of donor T-cells in MHC mismatched transplants * C. Giver, R. O. Montes, S. Mittelstaedt, S. Lonial, J. M. Li, M. W. Boyer, E. K. Waller - Atlanta, U.S.A.

201

Immuno-regulatory role of donor CD11b+ cells in allogeneic BMT: effects on immune reconstitution, GVL and GVHD * J. Li, C. R. Giver, E. K. Waller - Atlanta, U.S.A.

202

PCR Negativity after therapy correlates with favourable prognosis in patients with B-Cell lymphoproliferative disorder * J. Cerny, M. Trneny, A. Slavickova, V. Valkova, R. Pytlik, J. Salkova, R. Ivanek, P. Klener - Prague 2, Czech Republic

203

Developing a methodology to detect human T cell receptor variable family gene expression in graft-vs-host disease patients * J. Brewer, S. Ericson - Morgantown, U.S.A.

16:30 - 18:00

POSTER SESSION

29 Signal Transduction and Granulopoiesis 204

Age-related change in myelopoietic response to lipopolysaccharide in senescence-accelerated mice(SAM) * T. Tsuboi, U. Sawada, T. Horie, M. Hiramoto, S. Aizawa - Itabashi-ku, Japan

205

Alterations in a GM-CSF Signaling Pathway by the Benzene Metabolite Hydroquinone J. Zheng, *S. Gross, D. Pyatt, P. Kerzic, R. Irons - Denver, U.S.A.

206

Eosinophilopoiesis in Livers of Mice with Transgenic Overexpression of Interleukin-5 * M. McGarry, D. Dimina, E. Hines, N. Lee, J. Lee - Scottsdale, U.S.A.

207

Macrophages determine neutrophil granulocyte kinetics and ecotaxis (“homing”)in rats with a sterile peritonitis E. Knudsen, P. O. Iversen, T. Seierstad, N. van Rooijen, *H. Benestad - Blindern, Norway

208

Selection and cloning of gene transfected cells in one single culture * P. Orchansky, J. Yu, G. Trigo-Gonzalez, C. Miller, A. Eaves - Vancouver, Canada

209

The Causal Role of Mutations in the Neutrophil Elastase Gene in Cyclic and Congenital Neutropenia * A. Aprikyan, S. Stein, T. Kutyavin, E. Rodger, A. Bolyard, D. Dale - Seattle, U.S.A.

Program / Sunday, July 7

198

High doses of allogeneic peripheral blood CD34+ stem cells (PBSC) correlate with better engraftment but induce a higher rate of extensive chronic GVHD * M. Mohty, K. Bilger, E. Jourdan, M. Kuentz, M. Michallet, J. Bourhis, N. Milpied, L. Sutton, J. Jouet, D. Maraninchi, N. Fegueux, D. Blaise - Marseille, France

18

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Sunday, July 7, 2002 16:30 - 18:00

POSTER SESSION

Program / Sunday, July 7

30 Leukemia - Experimental and Clinical 210

Potential for therapy with leukemic dendritic cells derived from patients with acute myeloid leukemia * M. Mohty, D. Blaise, D. Isnardon, D. Olive, B. Gaugler - Marseille, France

211

Novel Inhibitors of Multidrug Resistance (MDR)- Existence of Complex, Multiple Interaction Sites on P-glycoprotein. * H. Galski, A. Nagler - Tel Hashomer, Israel

212

Upregulation of CD20 expression in B-lineage malignant cells by bryostatin 1 * A. Al-Katib, S. Ge, A. Aboukameel, M. Dandashi, R. Mohammad - Detroit, U.S.A.

214

Survival advantage for mice with Friend Virus-induced erythroleukemia, by combination treatment of chronic low-dose chemotherapy and a selective COX-2 inhibitor * D. Cervi, G. Klement, A. Koki, Y. Ben-David - Toronto, Canada

215

Generation of dendritic cells from acute myelogenous leukemia patients is affected by clinical aggressiveness and cellular VEGF level * J.-J. Lee, C.-E. Nam, I.-J. Chung, Y. Takaue, H.-J. Kim - Gwangju, Korea

216

DNA repair capability is important in determining bryostatin-enhanced 2CdA cytotoxicity in chronic lymphocytic leukemia F. Beck, A. Al-Katib, D. Eilender, M. Dandashi, *R. Mohammad - Detroit, U.S.A.

217

Inhibitory effect of bispecific anti-CD33 x anti-CD64 antibody on AML cells growth correlates with the Syk expression * L. Balaian, E. D. Ball, La Jolla - U.S.A.

218

Polymorphisms within glutathione S-transferase genes(GSTM1, GSTT1, GSTP1) and increasing risk of childhood acute lymphoblastic leukemia in Korea * C. J. Lyu, S. Won, C. Yang, S. Lee, B. Kim - Seoul, Korea

219

Primary non-Hodgin's B-cell lymphomas from distinct histological types are equally sensible to rituximabmediated apoptosis, phagocytosis and antibody-dependent cell cytotoxicity, but aredifferentially sensible to rituximab-mediated complement dependent cytotoxicity * O. Manches, G. Lui, L. Chaperot, J. Plumas - La Tronche, France

220

Phenotypic characterisation of ALL progenitor cells in vitro and in vivo * A. Blair, C. Cox, N. Goulden, R. Evely, D. Pamphilon - Bristol, United Kingdom

221

Optimising Culture Conditions for the Growth of Clonogenic Acute Lymphoblastic Leukaemia cells * C. Cox, N. Goulden, D. Pamphilon, A. Blair - Bristol, United Kingdom

222

Type 1 Cytokines Predominate in Whole Blood of Patients with Acute Myelogenous Leukaemia Whereas Type 2 Cytokines Prevail in Healthy Controls * N. Panoskaltsis, S. Knight, C. Reid - London, United Kingdom

223

Dendritic Cells Pulsed With Tumor Antigens Enhanced The Anti-Tumor Activity of The Cytokine Stimulated T Cells * S. Zhang, S. You, W. Ge, S. Ma, R. C. Zhao - Tianjin, China

224

Rationale for Combination Therapy of Chronic Myelogenous Leukemia with Imatinib and Irradiation or Alkylating Agents: Implications for Pretransplant Conditioning S. Fruehauf, *J. Topaly, M. Schad, A. Ho, W. Zeller - Heidelberg, Germany

20

Investigation of the downstream signals of the Erythropoietin Receptor leading to erythroid proliferation and differentiation * A. Truong, B. Zochodne, Y. Ben-David - Toronto, Canada

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

19

Scientific Program Sunday, July 7, 2002 Quantitative evaluation of PML/RARa chimeric transcripts in acute promyelocytic leukemia cells in reference to clinical features * A. Sato, M. Imaizumi, Y. Hoshi, M. Tsurusawa, K. Horibe, I. Tsukimoto, Y. Hayashi, K. Iinuma - Sendai, Japan

226

DAP-kinase and GSTP1 methylation pattern and prognostic relevance in secondary and de novo adult acute myeloid leukemia * M. T. Voso, A. Scardocci, F. D'Alo', R. Putzulu, S. Sica, L. Pagano, F. Lo Coco, S. Hohaus, G. Leone - Rome, Italy

227

Impaired responsiveness to all-trans retinoic acid in cellular or molecular level of UF-1, a human acute promyelocytic leukemia cell line established from a patient who acquired ATRA-resistance * M. Imaizumi, A. Sato, Y. Hoshi, K. Fujii, T. Rikiishi, S. Chikaoka, A. Kakizuka, K. Iinuma, Y. Hayashi - Sendai, Japan

228

Ex vivo expansion of CD3+56+ cytokine induced killer cells (CIK): from fresh or cryopreserved PBMCs samples R. Giancola, P. Olioso, M. Di Riti, *T. Bonfini, P. Accorsi, A. Iacone - Pescara, Italy

229

Fanconi Anemia Type C (FancC) Cooperates with Haploinsufficiency of Neurofibromin Type 1 in Spontaneous and Genotoxin Induced Leukemogenesis * X. Li, Y. Yang, E. Srour, K. Shannon, D. Clapp - Indianapolis, U.S.A.

230

Induction of apoptosis using a proteasome inhibitor/anthracycline combination specifically targets leukemic but not normal human stem cells * M. Guzman, S. Neering, R. Rossi, C. Swiderski, B. Grimes, D. Howard, D. Rizzieri, S. Szilvassy, C. Jordan - Lexington, U.S.A.

231

β) Inhibitor, Induces Apoptosis in Leukemia CT-32228: A Lysophosphatidic Acid Acyltransferase-Beta (LPAAT-β and Lymphoma Cell Lines * P. de Vries, J. Tulinsky, J. Pound, L. Romero, D. Hollenback, M. Coon, J. Singer - Seattle, U.S.A.

232

Identification of the functional domains of the oncogene TLS-Erg in the human leukemogenic process * J. Larking, J. Dick, Toronto, Canada

233

The RARa-PLZF fusion protein inhibits C/EBPa function in myeloid cells * N. Girard, A. Haman, N. Bouchard, J. Labrecque, B. Chen, Z. Chen, S.-J. Chen, T. Hoang - Montreal, Canada

234

PI-3K/Akt(PKB) pathway is critical for interleukin-7-mediated survival, activation and cell cycle progression of T-cell acute lymphoblastic leukemia cells * J. Barata, A. Cardoso, J. Brandao, L. Nadler, V. Boussiotis - Lisbon, Portugal

235

Different oncogenic properties of Hoxa9 and Hoxa10 in myeloid and lymphoid cells * J. Bijl, J. Vacher, G. Sauvageau - Montreal, Canada

236

Ectopic expression of HOX11 is not permissive for hemopoietic reconstitution in mice * M. Bilodeau, U. Thorsteinsdottir, E. Kroon, N. Mayotte, G. Sauvageau - Montreal, Canada

237

The ability of alpha-1-acid glycoprotein to bind to and affect the efficiency of drugs used in the treatment of chronic myeloid leukaemia * S. Paterson, K. Smith, P. Mooney, C. Loveridge, H. Jorgensen, T. Holyoake - Glasgow, United Kingdom

238

Synergistic effects of arsenic in combination with conventional drugs and ATRA in multidrugs resistant cell lines * S. Lehmann, S. Bengtzen, C. Paul - Stockholm, Sweden

239

The use of Glivec in chronic myeloid leukemia: Report of 10 cases in a single institution * M. Vasilica, R. Stoia, O. Georgescu, V. Codreanu - Bucharest, Romania

240 241

TMice with A CML-like disease relapse with ALL under STI571 treatment C. Miething, R. Grundler, J. Hoepfl, C. Mugler, H. Gschaidmeier, C. Peschel, *J. Duyster - Munich, Germany Induction of growth inhibition, apoptosis and accumulation of unprocessed N-RAS by co-treatment of myeloid leukemia cells with farnesyltransferase and geranylgeranyltransferase-I inhibitors * M. Morgan, J. Wegner, A. Ganser, C. Reuter - Hannover, Germany

Program / Sunday, July 7

225

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Sunday, July 7, 2002 16:30 - 18:00

POSTER SESSION

Program / Sunday, July 7

31 Myelodysplastic Syndrome 242

Studies on mitochondrial DNA mutations in chronic myelomonocytic leukemia patients * P. Reddy, S. Suleman, M. Khan, S. Sarkaria, S. Anthwal, V. Shetty, S. Dar, S. Mundle, K. Allampallam, S. Alvi, N. Galili, G. Saberwal, M. Shaikh, S. Kamal, A. Arora, R. Jafri, A. Raza - Chicago, U.S.A.

243

Comparative analysis of hypermethylation of cell cycle control genes in low-density and CD34+ bone marrow cells from patients with myelodysplastic syndrome * W.-K. Hofmann, S. Takeuchi, N. Takeuchi, D. Hoelzer, H. Koeffler - Frankfurt/Main, Germany

244

Tumor necrosis factor-a induced maturation of monocyte-derived dendritic cells is deficient in patients with myelodysplastic syndromes * I. Micheva, M. Karakantza, T. Kakayanni, S. Michalopoulu, A. Galani, M. Tiniakou, A. Kourakli, N. Zoumbos - Athens, Greece

245

Analysis of T cell receptor Vß repertoire in patients with myelodysplastic syndrome * K. Iwabe, N. Shimoyama, M. Ishiyama, H. Kazama, M. Teramura, T. Kato, H. Mizoguchi - Tokyo, Japan

16:30 - 18:00

POSTER SESSION

32 Myeloma 246

Differential regulation of dendritic cell function by the immunomodulatory drug thalidomide: implications for multiple myeloma therapy * M. Mohty, D. Blaise, D. Isnardon, D. Olive, B. Gaugler - Marseille, France

247

Dose-Dense Therapy with L-Phenylalanine Mustard for Multiple Myeloma:Interim Report * G. Elfenbein, L. Lum, R. Rathore, S. Malik, M. Falvey - Providence, U.S.A.

248

A Phase I Study of an Immunomodulatory Thalidomide Analogue (CC4047) in Relapse/Refractory Multiple Myeloma * S. Schey, R. Jones, K. Raj, M. Streetley - London, United Kingdom

249 250

Angiogenetic properties of human myeloma cells: Role of angiopoietin system S. Colla, *N. Giuliani, M. Moroni, R. Sala, S. Bonomini, M. Lazzaretti, G. Sammarelli, V. Rizzoli - Parma, Italy Human myeloma cells stimulate the receptor activator of NF-KB Ligad (RANKL) in T cells: Role of interleukin-7 * N. Giuliani, S. Colla, M. Moroni, S. Bonomini, M. Lazzaretti, G. Sammarelli, S. La Monica, R. Bataille, V. Rizzoli - Parma, Italy

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

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Scientific Program Sunday, July 7, 2002 16:30 - 18:00

POSTER SESSION

33 Oncogenes, Tumor Suppressor Genes, and Negative Regulators SHIP deficient murine peritoneal macrophages are impaired in LPS/IFNgamma-induced NO secretion because of a dramatic overproduction of L-arginase * M. Rauh, C. Pereira, M. Lane, J. Damen, W. Murphy, A. Mui, G. Krystal - Vancouver, Canada

252

Gradient of E2A activity in B cell development * S. Herblot, P. Aplan, T. Hoang - Montreal, Canada

253

Isolation of CD4/CD8 double positive regulatory T cells that are specific for a minor histocompatibility antigen A. Farre, M. Michallet, D. Laurin, J. Bienvenue, D. Rigal, L. Gebuhrer, *A. Eljaafari - Lyon, France

254 255

Withdrawn Abnormal Transcripts of the FHIT Gene In Non-Hodgkin’s Lymphoma * P.-M. Chen, C.-J. Chu, M.-H. Yang, T.-J. Chiou, C.-C. Yen, W.-S. Wang, J.-H. Liu, Y.-C. Lin - Taipei, Taiwan

158

Combined immunodeficiency (CID) due to lack of expression of CD80 by B-cells * M. Ege, S. Müller, W. Friedrich, K. Debatin, K. Schwarz - Ulm, Germany

16:30 - 18:00

POSTER SESSION

34 Radiation Effects 256 257

258 259

191

16:30 - 18:00

Bone-marrow hemopoiesis in the areas exposed to combined chemo- and radiotherapy * I. Isayev, A. D. Ismailov - Baku, Azerbaijan Mesenchymal stem cells sustain expansion of 4 Gy g in vitro irradiated CD34+ cells in presence of SCF + FL + TPO + IL-3 F. Mourcin, N. Grenier, J. Mayol, V. Leroux, P. Bourin, *M. Drouet, F. Hérodin - La Tronche, France Bone marrow cells contribute to irradiation-induced pulmonary fibrosis * M. Epperly, H. Guo, J. Greenberger - Pittsburgh, U.S.A. Mitochondrial transmembrane potential allows to assess radiation-induced apoptosis in vivo in bone marrow CD34+ cells J. Mayol, J. Mathieu, F. Mourcin, *F. Herodin, M. Drouet - La Tronche, France Ionizing radiation promotes the expansion of TH-2-type cells which persist for a protracted time * J. Albanese, L. Karkanitsa, S. Kreczko, N. Dainiak - Kalamazoo, U.S.A. POSTER SESSION

35 Red Blood Cell Disorders 260

Atypical Blood Group Antibidies in Blood Transfusion Practice * A. Pourazar, S. Joshi, R. Nassiri - Isfhan, Iran

261

The characteristics of megalobalstic anemia associated with thalassemia * C.-H. Ho, C. Tzeng, E. Lin - Taipei, Taiwan

262

Nitric Oxide Increases Ferritin Synthesis in Macrophages and Changes Cellular Iron Distribution: Molecular Mechanism of Anemia of Chronic Disorders * S. Kim, P. Ponka - Montreal, Canada

Program / Sunday, July 7

251

22

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Sunday, July 7, 2002 16:30 - 18:00

POSTER SESSION

36 Transcription Factors and Thrombopoiesis

Program / Sunday, July 7

263

Bone Marrow and Peripheral Blood C-Kit Ligand Concentrations in Patients with Thrombocytosis and Thrombocytopenia M. Asik, *I. Haznedaroglu, S. Karakus, S. Aksu, H. Kocoglu, Y. Buyukasik, H. Goker, D. Ertugrul, N. Sayinalp, O. Ozcebe, S. Kirazli, S. Dundar - Ankara, Turkey

264

Human Sphingosine Kinase 1: its Localization and Transcriptional Control * T. Murate, Y. Banno, K. T. Koizumi, N. Mori, A. Wada, Y. Igarashi, A. Takagi, Y. Nozava - Nagoya, Japan

265

Early and late megakaryocyte engraftment and platelet production by fresh and ex-vivo expanded human cord blood cd34+ cells * W. Piacibello, S. Bruno, F. Sanavio, F. Fagioli, M. Aglietta - Candiolo, Italy

266

Angelica Polysaccharide Enhances Megakaryocytopoiesis and Platelet Production In Mice * M. Yang, C. Lau, K. Li, N. Pong, S. Cheng, K. Fung, T. Fok, Y. Sung - Shatin, Hong Kong

267

In vivo treatment with thrombopoietin (TPO) does not correct thrombocytopenia in GATA-1low mice * A. R. Migliaccio, L. Bianchi, F. Paoletti, A. Salati, E. Alfani, M. Sanchez, G. Migliaccio, A. Vannucchi - Rome, Italy

269

High expression of stanniocalcin-1 in megakaryocytes and platelets M. Serlachius, R. Alitalo, H. Olsen, *L. Andersson - Helsinki, Finland

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

23

Scientific Program Monday, July 8, 2002 08:00 - 09:30

PLENARY SESSION

37 Marrow and Blood Stem Cell Transplantation Chair: Norbert-Claude Gorin (Paris, France), Denis-Claude Roy (Montreal, QC, Canada) Non-myeloablative Allogeneic Transplantation for Hematologic Malignancy * S. Forman - Duarte, U.S.A.

271

Autologous Stem Cell Transplantation in Non-Hodgkin's Lymphomas * N. Gorin - Paris, France

272

Extrathymic T Cell Development * C. Perreault, M. Blais, G. Gérard, I. Louis, R. Terra - Montreal, Canada

273

Potentials for tissue repair by hematopoietic stem cell transplant * S. Sharkis - Baltimore, U.S.A.

274

Visualization of tumor and effector cell trafficking in hematologic malignancies * R. Negrin, M. Edinger, M. Verneris, Y.-A. Cao, M. Bachmann, C. Contag - Stanford, U.S.A.

10:30 - 12:30

SIMULTANEOUS SESSION

MARQUETTE

38 Stem Cell Transplantation - Experimental Chairs: Saul Sharkis (Baltimore, MD, U.S.A.), Graca M Almeida-Porada (Reno, NV, U.S.A.) 275

Factors inherent to the cord blood stem cell graft determine telomere dynamics after transplantation to NOD/SCID mice H. Roelofs, W. Noort, A. Zwinderman, R. Willemze, *W. Fibbe - Leiden, The Netherlands

276

Increased time interval between radiation and transplantation negatively impacts homing and engraftment of primitive hematopoietic progenitor cells P. Plett, *C. Orschell - Indianapolis, U.S.A.

277

Hemangioblasts derived from embryonic stem (ES) cells can reconstitute hematopoiesis in vivo in SCID mice * T. Miyagi, M. Takeno, M. Takahashi, N. Suzuki - Kawasaki, Japan

278

Flow cytometric assay for counting of dendritic cells type-1 (DC1) and dendritic cells type-2 (DC2) in whole blood M. Zysk, A. Dzionek, *J. Schmitz - Bergisch Gladbach, Germany

279

Polymorphonuclear Neutrophil (PMN) Dependent and IndependentMobilization of Hematopoietic Stem and Progenitor Cells into Peripheral Blood in Mice * L. Pelus, H.-M. Bian, S. Fukuda - Indianapolis, U.S.A.

280

Expansion And Exhaustion Of Hematopoietic Stem Cells By Serial Transplantation * L. Kamminga, E. Dontje, G. de Haan - Groningen, The Netherlands

281

Successful engraftment of Chronic Myelomonocytic Leukemia cells in NOD/SCID mice requires granulocytemacrophage colony-stimulating factor (GM-CSF) * H. Ramshaw, P. Bardy, A. Lopez - Adelaide, Australia

282

Stroma cell-dependent transdifferentiation of bone marrow stem cells in uterus * S. Okamoto, H. Tamura, Y. Futamata, K. Tamura, T. Hara - Tokyo, Japan

Program / Monday, July 8

270

24

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 10:30 - 12:30

SIMULTANEOUS SESSION

JOLLIET

39 Transcription Factors Chairs: Trang Hoang (Montreal, QC, Canada), Alan M. Gewirtz (Philadelphia, PA, U.S.A.)

Program / Monday, July 8

283

The SCL-complex regulates c-kit expression in hematopoietic cells through functional interaction with Sp1 * E. Lecuyer, S. Herblot, M. St-Denis, R. Martin, C. Begley, C. Porcher, S. Orkin, T. Hoang - Montreal, Canada

284

Notch signalling directly upregulates PU.1 expression and induces multilineage myeloid differentiation T. Schroeder, H. Kohlhof, N. Rieber, *U. Just - Muenchen, Germany

285

Runx1 dependent IGFBP-3 suppression in the cell line derived from the Aorta-Gonad-Mesonephros region of mouse embryo * K. Iwatsuki, S. Takahashi, Y. Futamata, T. Hara - Tokyo, Japan

286

Enforced expression of HoxB4 in human primitive hematopoietic progenitors determines cell fate in a concentration dependent manner * A. Brun, X. Fan, J. Bjornsson, K. Humphries, S. Karlsson - Lund, Sweden

287

Kit-ligand inactivates the Forkhead transcription factors FKHR and FKHRL1 via phosphoinositide 3-kinase/PKB(Akt) in multipotent progenitor cells * M. Engstrom, R. Karlsson, J. I. Jonsson - Malmo, Sweden

288

Regulation of a sub-set of HOX genes by ATRA in the Acute Promyelocytic Leukemia cell line, NB4 * C. O’Neill, M. McMullin, T. Lappin, A. Thompson - Belfast, United Kingdom

289

Acquired mutations in GATA1 in the megakaryoblastic leukemia of Down syndrome J. Wechsler, M. Greene, M. McDevitt, J. Anastasi, J. Karp, M. Le Beau, *J. Crispino - Chicago, U.S.A.

290

Interaction and Cooperation of MITF and MAZR for Transcription of Mouse Mast Cell Protease 6 Gene * E. Morii, Y. Kitamura - Suita, Japan

291

NF-kB transcriptional factor is involved in the regulation of the Wilms’ tumor gene expression * D. Cilloni, F. Messa, A. Morotti, E. Messa, M. Fava, E. Gottardi, G. Saglio - Torino, Italy

292

Molecular Cloning and Characterization of a Novel Hematopoietic Marker Expressed in Early Embryonic Stem Cells Through Mature NK Cells * M. Kirszenbaum, S. Prost, R. Maddad, J. Gluckman, M. Le Discorde, B. Canque - Fontenay aux Roses, France

10:30 - 12:30

SIMULTANEOUS SESSION

DULUTH

40 Stem Cell Transplantation - Clinical Chairs: Axel R. Zander (Hamburg, Germany), Angelo A. Cardoso (Boston, MA, U.S.A.) 293

Autologous stem cell transplantation followed by a dose-reduced allograft induces high complete remission rate in multiple myeloma * N. Kröger, R. Schwerdtfeger, M. Kiehl, H. Sayer, T. Zabelina, B. Fehse, R. Kuse, G. Wittkowsky, A. Zander - Hamburg, Germany

294

Allogeneic stem cell transplantation from unrelated donors after a dose-reduced conditioning in patients with advanced multiple myeloma * N. Kröger, R. Schwerdtfeger, A. Nagler, M. Kiehl, H. Sayer, H. Renges, T. Zabelina, B. Fehse, A. Zander - Hamburg, Germany

295

Lymphocyte recovery and age: determinant factors of survival after autologous peripheral stem cell transplantation in hematologic malignancies * A.-L. Herr, M. Edwardes, S. Lachance, P. Laneuville, J. Routy - Montreal, Canada

296

Universal immunotherapeutic approach for patients with refractory malignancies: HLA-haploidentical transplants in 100 cGy-conditioned hosts * G. Colvin, J.-F. Lambert, L. Lum, R. Rathore, P. Quesenberry, G. Elfenbein - Providence, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

25

Scientific Program Monday, July 8, 2002 Dendritic cells numbers and their subsets during the treatment in multiple myeloma * L. Kovarova, A. Svobodnik, T. Büchler, M. Klabusay, R. Hajek - Brno, Czech Republic

298

GvH-prophylaxis with ATG (Fresenius) in recipients of matched and mismatched unrelated donor transplants for chronic myelogenous leukemia: A retrospective analysis * A. R. Zander, M. Schleuning, N. Kroeger, J. Finke, T. Zabelina, J. Berger, D. Beelen, R. Trenschel, R. Schwerdtfeger, H. Baurmann, M. Bornhaeuser, G. Ehninger, A. Fauser, M. Kiehl, H. Kolb, U. Schaefer - Hamburg, Germany

299

Intramyocardial inoculation of autologous bone marrow cells in patients with refractory myocardial ischemia * A. Porcellini, B. Reimers, G. Azzarello, P. Pascotto, O. Vinante - Noale, Italy

300

Peripheral T Cell Levels Correlate With Outcome In Patients Undergoing Autologous Stem Cell Transplant * S. Rosinski, I. McNiece, E. Shpall, N. Clough, P. Russel, J. McDermott, Y. Nieto - Denver, U.S.A.

301

Allogeneic immunotherapy in 46 patients suffering from advanced solid tumors (ST) * D. Blaise, J. Bay, M. Michallet, J. Boiron, J. Cahn, N. Ifrah, J. Sotto, N. Gratecos, C. Faucher, J. Blay, D. Olive, D. Maraninchi, P. Viens - Marseille, France

302

Comparison of 2 different high-dose protocols in patients with breast cancer (BC) undergoing autologouse stem cell transplantation (ASCT) * A. Uss, V. Zmatchinski, E. Zhavrid, A. Putirski - Minsk, Belarus

10:30 - 12:30

SIMULTANEOUS SESSION

MACKENZIE

41 Functional Genomics, Oncogenes and Clonal Evolution Chairs: Guy Sauvageau (Montreal, QC, Canada), Arthur J Sytkowski (Boston, MA, U.S.A.) 303

Isolation and rapid mapping of recessive mutations affecting murine hemopoiesis * B. Kile, A. Bradley, R. Behringer, M. Justice - Houston, U.S.A.

304

Genetic Regulation of Hematopoietic Stem Cell Telomere Length in Mice * E. Manning, J. True, E. Henckaerts, H. Snoeck, H. Geiger, G. de Haan, L. Lu, R. Williams, G. Van Zant - Lexington, U.S.A.

305

Complex clustered gene expression patterns in hematopoietic stem cells * L. Bystrykh, E. Weersing, B. Dontje, E. Vellenga, G. de Haan - Groningen, The Netherlands

306

HoxB4 and HoxB3 control the proliferative capacity of hematopoietic stem cells * J. Bjornsson, N. Larsson, A. Brun, M. Magnusson, K. Humphries, S. Karlsson - Lund, Sweden

307

The Polycomb-Group (PcG) gene eed is a negative regulator of bone marrow progenitor cell proliferation and suppresses radiation-induced lymphomagenesis * J. Lessard, S. Girard, A. Schumacher, G. Sauvageau - Montreal, Canada

308

Haploinsufficiency of the Nf1 Tumor Suppressor Gene Enhances the Migration of Mast Cells Via Increased Adhesion to VLA-4 and Hyperactivation of the Ras-PI-3K-Rac2 Pathway * D. Ingram, S. Chen, L. Fisher, M. Wenning, H. Huddleston, S. Atkinson, D. Williams, R. Kapur, D. Clapp - Indianapolis, U.S.A.

309

Constitutive c-jun N-terminal kinase activity in acute myeloid leukemia derives from activated Flt3: JNK inhibition unleashes catastrophic oncogene stress response * A. Hartman, G. Burgess, C. Phillips, M. Su, F. Salituro, J. Alam, V. Gelfanov, H. Ramsey, L. Cripe, R. Hromas, H. S. Boswell Indianapolis, U.S.A.

310

Overexpression of EGR-1 and TAXREB107 in clonal cells of PNH patients * J. Schubert, A. Lyakisheva, R. Schmidt, A. Ganser - Hannover, Germany

311

Clonal Adaptation and Evolution in Fanconi Anemia (FA) A. Speckhart, M. Lensch, J. Yates, *G. Bagby, Portland, U.S.A.

268

BclxL Overexpression on megakaryocytes leads to impaired platelet fragmentation Y. Kaluzhny, G. Yu, S. Sun, P. Toselli, B. Nieswamdt, C. Jackson, *K. Ravid - Boston, U.S.A.

Program / Monday, July 8

297

26

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 13:30 - 15:00

42 New Investigator Award Nominee Presentations

LE GRANDE SALON

Program / Monday, July 8

Chairs: Arthur A Axelrad (Toronto, ON, Canada), Nicholas Dainiak (Bridgeport, CT, U.S.A) 312

Highly efficient lentiviral transduction of NOD/SCID repopulating cells but not long-term repopulating cells in the baboon in a direct comparison of the NOD/SCID assay versus autologous transplantation * P. Horn, B. Thomasson, J. Morris, H.-P. Kiem - Seattle, U.S.A.

313

Extensive heterogeneity of AML stem cells revealed by clonal tracking * K. Alexander, L. Jin, J. Dick - Toronto, Canada

314

hTERT gene expression is increased by alternative splicing of mRNA during the course of CML, and telomere loss at diagnosis is associated with early progression * M. Drummond, S. Hoare, M. Alcorn, S. Graham, W. Keith, T. Holyoake - Glasgow, United Kingdom

315

Interleukin-10 inhibits macrophage activation through induction of SOCS3 P. Qasimi, A. Ghanipour, A. Yoshimura, J. Ihle, *A. Mui - Vancouver, Canada

316

A New Adult Mouse Model of Lethal b0-Thalassemia and Its Rescue by Lentivirus-Mediated Globin Gene Transfer * S. Rivella, C. May, A. Chadburn, I. Riviere, M. Sadelain - New York, U.S.A.

317

Role of mitogen-activated protein kinase family in serum-induced leukemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells * T. Nakao, S. Kim, K. Ohta, H. Kawano, T. Asai, M. Hino, K. Miura, N. Tatsumi, H. Iwao - Abeno-ku, Japan

15:30 - 16:30

SIMULTANEOUS SESSION

MARQUETTE

43 Gene Therapy Chairs: W. French Anderson (Bethesda, MD, U.S.A.), David M. Bodine (Bethesda, MD, U.S.A.) 318

Complete chimerism with persistent multilineage transgene expression after immunoselection of retrovirally engineered hematopoietic stem cells * B. Fehse, Z. Li, B. Schiedlmeier, J. Düllmann, O. Frank, A. Zander, W. Ostertag, C. Baum - Hamburg, Germany

319

In vivo selection of human clonogenic cells and lymphomyeloid lineages derived from SCID-repopulating cells by expression of a mutant O6-methylguanine DNA methyltransferase (MGMTP140K) * K. Pollok, J. Hartwell, A. Braber, M. Jansen, E. Kreklau, L. Erickson, D. Williams - Indianapolis, U.S.A.

320

Retroviral-mediated gene transfer in human primary T lymphocytes: impact of the type of stimulation on TCRB repertoire of gene-modified cells * S. Coito, D. Sauce, A. Duperrier, J.-M. Certoux, F. Kuttler, A. Collette, E. Robinet, P. Tiberghien, C. Ferrand - Besancon, France

321

Tight regulation of plasmid based gene expression in vivo following intramuscular injection * M. Margalith, A. Vilalta, P. Hobart - San Diego, U.S.A.

322

Enhanced Repopulating Ability and Protection from Aberrant Proliferation in Fancc -/- Hematopoietic Stem Cells Transduced with Recombinant Fancc * L. Haneline, X. Li, P. Hong, A. Orazi, E. Srour, D. Clapp - Indianapolis, U.S.A.

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

27

Scientific Program Monday, July 8, 2002 15:30 - 16:30

SIMULTANEOUS SESSION

JOLLIET

44 Gene Expression Chairs: Jan A. Nolta (St. Louis, MO, U.S.A.), Gerard Wagemaker (Rotterdam, The Netherlands) A genetic and genomic analysis identifies a cluster of genes associated with hematopoietic cell turnover * L. Bystrykh, E. Weersing, B. Dontje, H. Geiger, N. Ivanova, I. Lemischka, E. Vellenga, G. Van Zant, G. de Haan - Groningen, The Netherlands

324

Comparison of Gene Expression Patterns of Erythroid and Myeloid Differentiation from Human Multipotential Progenitor Cells * L. Chen, J. Zhang, D. Tang, E. Fibach, Y. Shi, G. Rodgers - Bethesda, U.S.A.

325

Purified Hematopoietic Stem Cells Show a Major Shift of Gene Expression Through Cell Cycle that Coincides with an Engraftment Nadir * J.-F. Lambert, M. Liu, N. V. Baskaran, G. Colvin, C. I. Mc Auliffe, B. G. Forget, S. M. Weissman, P. J. Quesenberry Providence, U.S.A.

326

Identification of leukemia inhibitory factor (LIF)-responsive genes in embryonic stem (ES) cells bearing a Shp-2 deletion mutation using microarray analysis * R. Chan, W. Shelley, J. McClintick, H. Edenberg, M. Yoder - Indianapolis, U.S.A.

327

Microarray Analysis Reveals that Upregulated Anti-apoptositic andProliferative Molecules, and Downregulated Pro-apoptotic Gene Expression are Present in Human Leukemic Cells Stimulated by TNF-alpha * R. Liu, F. Fan, X. Li, B. Whitehouse, K. Zuckerman - Tampa, U.S.A.

15:30 - 16:30

SIMULTANEOUS SESSION

DULUTH

45 Graft vs. Host Disease Chairs: Alan W. Flake (Philadelphia, PA, U.S.A.), Catherine M. Verfaillie (Minneapolis, MN, U.S.A.) 328

Long-term chimerism from T-cell depleted allogeneic bone marrow transplantation with low-dose busulfan and CD40-CD154 blockade * J. Li, J. Gorechald, C. Larsen, E. Waller - Atlanta, U.S.A.

329

Mixed Hematopoietic Chimerism – A New Strategy for Preventing GVHD in Nonmyeloablative Allogeneic Stem Cell Transplantation * H. Ai, R. C. Zhao, M. Guo, C. Yu, D. Wang, J. Qiao, Q. Sun, W. Sun, S. Zhang - Beijing, China

330

Characterization of alloreactive T-cell clones by CDR3-size spectratyping in a murine GVHD model after transplantation over minor Histocompatibility antigen mismatches * K. Schilbach, J. Pippir, H. Fluhr, K. Marquordt, B. Schütt, D. Niethammer, M. Eyrich - Tuebingen, Germany

331

Specific elimination of alloreactive T lymphocytes by TH9402 based photodynamic therapy * R. Terra, G. Krosl, A. Balassy, M. Barrette, J. Rooney, D. Roy - Montréal, Canada

332

Tacrolimus and Mycophenolate Mofetil for GVHD prevention in Adult Hematopoietic Allograft Recipients * P. McSweeney, S. Abhyankar, B. Blunk, A. Baron, J. Foran, J. McGuirk, P. Hardiman, S. Picken, S. Pavletic - Denver, U.S.A.

Program / Monday, July 8

323

28

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 15:30 - 16:30

SIMULTANEOUS SESSION

MACKENZIE

46 Late Breaking Abstracts

Program / Monday, July 8

Chairs: Ihor R. Lemischka (Princeton, NJ, U.S.A.), Peter J. Quesenberry (Providence, RI, U.S.A.) 333

The Role of G-Protein Signaling in Hemopoietic Stem/Progenitor Cell Mobilisation * T. Papayannopoulou, G. Priestley, B. Nakamoto - Seattle, U.S.A.

334

Potential role of Grb2 in sequestering P27Kip1 in the nuclear compartment of BCR/ABL-cells * M. Dao, C. Verfaillie - Minneapolis, U.S.A.

408

Increased expression of Gibbon Ape Leukemia Viral receptor on K562 cells and human CD34+ cells reverses inhibition of gene transfer seen in the presence of high concentrations of GALV pseudotyped vector * T. Relander, A. Brun, L. Pedersen, K. Olsson, J. Richter - Lund, Sweden

336

Mechanism(s) of Matrix Metalloproteinase-2 Activation in Acute Myelogenous Leukemia L. Marquez, L. Ross, N. Shrivaikar, L. Larratt, A. Turner, *A. Janowska-Wieczorek - Edmonton, Canada

337

Cleavage of FLIPlong and Caspase-8 Are Associated With CD44-Mediated Induction of Apoptosis in CD4 Memory Cells J. Panse, U. Platzbecker, T. Gooley, E. Santos, H. Deeg, *B. Sandmaier - Seattle, U.S.A.

16:30 - 18:00

POSTER SESSION

47 Stem Cell Biology 338

GATA-transcription in a small Rho123 low, CD34+ subpopulation of peripheral blood derived CD34- CD105+ adult stem cell lines * C. Conrad, B. Gottgens, S. Kinston, R. Huss - Munich, Germany

339

The Role of flt3 and EPO Receptor Expressions on CD34+ Hematopoietic Stem Cell Differentiation and Proliferation. * J. Xu, H.-N. Hao - Jinan, China

340

Isolation of a Mast Cell Progenitor Dependent Upon SCF and IL-3: A Model of Stem Cell Signalling * I. McNiece, J. Brunetti, Z. Dai - Denver, U.S.A.

341

One third of mice transplanted with single rhodamine123-negative SP cells isolated from adult mouse bone marrow produce multilineage populations of blood cells for >6 months * N. Uchida, F. Leung, C. Eaves - Vancouver, Canada

342

Phenotypic and genetic analysis of stem cell number and mobilization potential in genetically distinct mouse strains E. J. Noach, A. Ausema, I. Akkerman, S. Koopal, E. Weersing, E. Dontje, *P. Wierenga, E. Vellenga, G. de Haan - Groningen, The Netherlands

343

Rhodamine123 exclusion identifies immature hematopoietic progenitors after short-term culture M. von Planta, D. Suva, M. Duchosal, B. Chapuis, *V. Kindler - Geneva, Switzerland

344 345

CD34 is a Marker of Mature Murine Mast Cells * K. McNagny, E. Drew, H. Merkens, S. Chelliah, R. Doyonnas - Vancouver, Canada Mobilized Peripheral Blood (MPB) as well as Bone Marrow (BM) can be used to generate neuron-like cells in vitro T. Tondreau, L. Lagneaux, A. Delforge, *D. Bron - Brussels, Belgium

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

29

Scientific Program Monday, July 8, 2002 Activation of telomerase in bone marrow cells after thymectomy of adult mice * T. Todria, A. Zander - Moscow, Russian Federation

347

Thioredoxin overexpression mouse is less sensitive to benzene hematotoxicity and leukemogenicity * Y. Hirabayashi, B. Yoon, Y. Kawasaki, J. Yodoi, T. Inoue - Tokyo, Japan

348

Aryl hydrocarbon receptor (AhR) mediates benzene-induced hematotoxicity * B.-I. Yoon, Y. Hirabayashi, J. Kanno, Y. Fujii-Kuriyama, T. Inoue - Setagayaku, Japan

349

Ex vivo amplification of human hematopoietic stem cells by using passive transduction of HOXB4 homeoprotein * S. Amsellem, F. Pflumio, D. Bardinet, P. Charneau, A. Dubart-Kupperschmitt, S. Fichelson - Paris, France

350

Ex Vivo Expansion of CD34+ Stem Cells in HSCEM/Stemline(TM) Medium Leads to Increased Levels of Total Nucleated Cells and CD34+ Cells * F. Swartzwelder, J. D. Tario, Jr., D. W. Allison, S. L. Leugers, H. N. Loke, L. M. Donahue, J. A. Harrington, I. K. McNiece Amherst, U.S.A.

351

Differential expression of alpha2 integrin separates long-term and short-term reconstituting Lin(-)Thy1.1(lo)c-kit(+)Sca-1(+) hematopoietic stem cells * A. Wagers, I. Weissman - Stanford, U.S.A.

352

Constitutive Circulation of HSC in Fetal Blood * J. Christensen, D. Wright, I. Weissman - Stanford, U.S.A.

353

Cytokine deprivation improves the homing of long-term cultured human hematopoietic stem cells after transplantation int NOD/SCID mice * T. Kerre, B. Vandekerckhove, F. Offner, J. Plum - Gent, Belgium

354

Identification and Characterization of Multipotential Stem Cell in the Human Dorsal Root Ganglion F. Zhang, Y. Hu, Y. Tan, G. Hong, F. Wang, L. Li, E. Fan, *R. C. H. Zhao - Tianjin, China

355

Flk1+ CD34- Fetal BoneMarrow-derived Cells Having Characteristics of Hemangioblast H. Guo, Z. Zhao, *J. Liu, H. Chen, R. Zhao - Tianjin, China

356

A Phase Space Model of Hemopoiesis * M. Kirkland, K. Borokov - Geelong, Australia

357

Functional differences between individual hematopoietic stem cell candidates in vitro are correlated with telomere length measured by Flow-FISH * K. Bartolovic, B. Berner, S. Balabanov, A. Wahl, A. Marxer, L. Kanz, T. Brummendorf - Tubingen, Germany

358

Distinct characteristics of hematopoietic stem cells in AGM and fetal liver * M. Takeuchi, T. Sekiguchi, A. Miyajima - Bunkyou-ku, Japan

359

CD133: Internal & External Antigen Distribution by Confocal Laser Scanning Microscope Analysis and 3-Dimensional Imaging * K. Whiting, C. McGuckin, N. Forraz, D. Wertheim, R. Pettengell - London, United Kingdom

360

Highly sensitive ligation-mediated PCR technique demonstrates that multiple clones reconstitute human hematopoiesis in the bone marrow of NOD/SCID mice * S. Laufs, B. Gentner, K. Nagy, K. Kühlcke, J. Topaly, B. Schiedlmeier, W. Zeller, A. Ho, S. Fruehauf - Heidelberg, Germany

361

Ontogenic Fate of Mesenchymal Cell Associated Hepatic and Hematopoietic stem cells in the Mouse Liver (or Niches Save the Queen) * I. Blazsek, J. Chagraoui, E. Oberlin, G. Uzan, B. Péault - Villejuif, France

362

Contributions of spleen, liver, and bone marrow in maintaining hematopoietic homeostasis in the neonatal mouse * F. Wolber, E. Leonard, M. Yoder, E. Srour - Indianapolis, U.S.A.

Program / Monday, July 8

346

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31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 363 364

Program / Monday, July 8

365

Restricted transgene expression in human CD34+ cells mediated by novel lentiviral vectors Z. Ye, *L. Cheng - Baltimore, U.S.A. Functional properties of muscle-derived CD45-Sca-1+c-kit- cells fractionated on the basis of CD34 and Thy-1.2 expression * J. Howell, M. Yoder, E. Srour - Indianapolis, U.S.A. Non-peptide lipid mediators induce proliferation and adhesion of CD34+ hematopoietic progenitor cells A. Boehmler, C. Denzlinger, L. Kanz, *R. Möhle - Tübingen, Germany

366

Stem/Progenitor Cell Inversions:The Chiaroscuro Stem Cell Model of Hematopoiesis * G. Colvin, J.-F. Lambert, C. McAuliffe, P. Quesenberry - Providence, U.S.A.

367

Efficient single hematopoietic cell transplantation * S. Corbel, A. Lee, L. Yi, F. Rossi - Vancouver, Canada

368

Green Fluorescent Protein (GFP) positive marrow trafficking to injured murine liver * M. Abedi, G. Colvin, J. Lambert, C. McAuliffe, M. Dooner, P. Quesenberry - Providence, U.S.A.

369

Computational and functional approaches for use of bone marrow (BM) stem cells: Mesenchymal (MSC) and lymphohematopoietic (LHSC) for neuronal repair and/or replacement and identification of two molecular markers * P. Rameshwar, J. Potian, K. Sancilio, P. Bandari, L. Challenger, E. Homsi, J. McArdle - Newark, U.S.A.

370

Reconstitution of B-lymphocite repertoire after allogeneic hematopoietic stem cell transplantation in children analysis by HCDR3 fingerprinting * D. Di Martino, A. Valetto, M. Terranova, P. Di Michele, L. Scarso, M. Iannacchino, G. Morreale, E. Lanino, G. Dini - Genova, Italy

371

The marrow homing efficiency of murine hematopoietic stem cells remains constant during ontogeny * S. Szilvassy, P. Ragland, C. Miller, C. Eaves - Lexington, U.S.A.

372

Detection of human cell factors in liver tissue of chimeric goats engrafted with human hematopoietic stem cells * F. Zeng, H. Yam, C. Pang, S.-z. Huang - Philadelphia, U.S.A.

373

Characterization of Chromatin-binding protein HMG-4 Function in hematopoietic stem cells * M. Nemeth, D. Curtis, A. Cline, S. Anderson, L. Garett, D. Bodine - Bethesda, U.S.A.

374

The hematopoietic system, DNA integrity and longevity: a genetic connection * H. Geiger, J. True, G. van Zant - Lexington, U.S.A.

375

Comparison Study of Human Fetal and Adult Bone Marrow Derived Mesenchymal Stem Cells * Y. Hu, L. Ma, G. Ma, X. Jiang, R. C. Zhao - Tianjin, China

376

Modulation of the 1 alpha,25 dihydroxyvitamin D3 induced proliferation of a stem cell * D. Ben Alon, I. Nathan, G. Lugassy - Ashkelon, Israel

377

Flt3 expression within the Lin-Sca1+c-kit+ stem cell pool defines a novel progenitor population with lymphomyeloid but lineage-restricted reconstitution potential * J. Adolfsson, O. Borge, D. Bryder, K. Theilgaard-Mönch, I. Åstrand-Grundström, E. Sitnicka, S. Jacobsen, Y. Sasaki - Lund, Sweden

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

31

Scientific Program Monday, July 8, 2002 16:30 - 18:00

POSTER SESSION

48 Stem Cell Transplant - Experimental Human Hematopoietic Stem Cells Can Be Induced To Differentiate Into Astroglial Cells In Vitro * H.-N. Hao, J. Zhao, R. L. Thomas, W. Lyman - Detroit, U.S.A.

379

The Long-Term Engraftment of Cord Blood CD34+ Cells After Ex Vivo Culture * R. Brown, J. Zhang, G. Almeida-Porada, R. Herzig, E. D. Zanjani - Gaithersburg, U.S.A.

380

A Novel Technique for Long-term Cryopreservation of Hematopoietic Progenitor Cells using Intracellular Trehalose * S. Buchanan, S. Gross, J. Acker, M. Toner, J. Carpenter, D. Pyatt - Denver, U.S.A.

381

Biodistribution of Gene-marked Mesenchymal Stem Cells in Immunocompetent Fisher Rats * R. Alur, J. Hendricks, S. Campbell, P. Vanguri - Baltimore, U.S.A.

382

Marked regional stem/progenitor cell distribution differences within different marrow compartments * J.-F. Lambert, G. Colvin, J. Carlson, P. Quesenberry - Providence, U.S.A.

383

Co-culture with Human Brain Endothelial Cells Differentially Maintains Human Bone Marrow SCID-repopulating cells in Vitro in the Absence of Exogenous Cytokines * J. Chute, J. Park, V. Tanavde, D. Harlan, C. Civin - Bethesda, U.S.A.

384

Transplantation of Human hematopoietic stem cells into fetal goats under B-Scan ultrasonographic guidance: An attractive approach to Prenatal therapy * S.-Z. Huang, F. Zeng, Z. Ren, Y. Zeng - Shanghai, China

385

Selective depletion of donor-reactive cells asa nonmyeloablative conditioning for allogeneicbone marrow transplantataion * T. Prigozhina, O. Gurevitch, G. Elkin, S. Morecki, E. Yakovlev, S. Slavin - Jerusalem, Israel

386

Human hematopoietic stem cell transplantation to treat hereditary hepatic related disorders * G. Almeida-Porada, C. Porada, E. Zanjani, Reno, U.S.A.

387

Microchimerism and clinical evaluations in pigs submitted to sequential kidney and bone marrow transplantation as experimental protocol to ameliorate the organ tolerance * M. Bonfichi, E. Ferretti, C. Marseglia, F. Abbiati, M. Alessiani, P. Bernasconi, E. Alessandrino - Pavia, Italy

388

Hepatocyte growth factor increases human albumin expression in the livers of immune deficient mice transplanted with highly purified human hematopoietic stem cells * X. Wang, S. Ge, G. McNamara, Q. Hao, G. Crooks, J. Nolta - Los Angeles, U.S.A.

Program / Monday, July 8

378

32

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 16:30 - 18:00

POSTER SESSION

Program / Monday, July 8

49 Stem Cell Transplant - Clinical 389

Incidence and features of expanded large granular lymphocytes (LGL) following allogeneic stem cell transplantation: a long term analysis * M. Mohty, C. Faucher, N. Vey, C. Chabannon, D. Sainty, C. Arnoulet, B. Gaugler, D. Maraninchi, D. Olive, D. Blaise Marseille, France

390

Donor lymphocyte infusions for prevention of relapse after stem cell transplantation in adults with high risk acute lymphoblastic leukemia * R. Arnold, G. Massenkeil, O. Rosen, B. Doerken, D. Hoelzer - Berlin, Germany

391

High incidence of fatty liver and low serum testosterone level in long term surviving patients who underwent allogeneic and autologous bone marrow transplantation in childhood * Y. Yasuda, T. Shimizu, Y. Inoue, H. Yabe, S. Kato - Kanagawa, Japan

392

Consolidation following autologous stem cell transplant of non-Hodgkins and Hodgkins Lymphomas * J.-F. Lambert, G. Colvin, R. Rathore, L. Lum, M. Falvey, G. Elfenbein - Providence, U.S.A.

393

Recovery of lymphocyte and dendritic cell subsets following reduced intensity allogeneic bone marrow transplantation * M. Mohty, B. Gaugler, C. Faucher, D. Sainty, M. Lafage-Pochitaloff, N. Vey, R. Bouabdallah, F. Viret, D. Blaise, D. Olive Marseille, France

394

Toward allogeneic stem cell transplantation (ASCY) without transfusion V. Ivanov, C. Faucher, P. Ladaique, M. Mohty, K. Bilger, D. Sainty, C. Chabannon, N. Vey, *D. Blaise - Marseille, France

395

Glivec treatment for Ph-positive (Ph+) leukemias who relapsed before or after allogeneic stem cell transplantation (ASCT) with intensive monitoring of minimal residual disease (MRD) * M. Yamada, K. Miyamura, O. Sasaki, J. Kameoka, K. Meguro, T. Sasaki - Sendai, Japan

396

Unrelated hematopoietic stem cell transplantation for acute myeloidleukemia * N. Basara, L. Kraut, S. Guenzelmann, M. Koldehoff, M. Kiehl, A. Fauser - Idar-Oberstein, Germany

397

G-CSF administration after autologous peripheral blood stem cell transplantation (PBSCT). Yes or no? * P. Henon, M. Ojeba-Uribe, Y. Arkam, D. Bourderont, H. Sovalat - Mulhouse, France

398

Transplantation of Peripheral Blood Stem Cells as Compared with Bone Marrow from HLA-identical Siblings in Patients with Acute Myeloid Leukemia and Acute Lymphoblastic Leukemia * O. Ringden, M. Labopin, A. Bacigalupo, F. Mandelli, U. Schaefer, J. Vossen, H. Koc, E. Gluckman, F. Frassoni - Huddinge, Sweden

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

33

Scientific Program Monday, July 8, 2002 16:30 - 18:00

POSTER SESSION

50 Progenitor Cell Mobilization, Processing 399

Predictive Parameters for Mobilized Peripheral Blood CD34+ Progenitor Ccell Collection in Pediatric Patients * C. J. Lyu, T. Ham, S. Won, C. Yang, S. Lee, B. Kim - Seoul, Korea

400

Higher freezing concentrations and its effect on ANC and platelet recovery post infusion * M. Dozier, K. Dicke, J. Gandy, M. Patel - Arlington, U.S.A.

401

Thawed Umbilical Cord Blood (UCB) followed by ex vivo engineering with anti-CD3, IL-2, IL-7 & IL-12 followed by recryopreservation significantly expands and activates UCB cytotoxic lymphocytes (CTLs): Potential for UCB :donor lymphocyte infusion (DLI) post UCB transplantation * M. Cairo, J. Ayello, J. Kurtzberg, C. Van de Ven - New York, U.S.A. POSTER SESSION

51 Late Breaking Abstracts 402

Application of a human multidrug transporter (ABCG2) variant as a potential in vivo selectable marker in gene transfer to haematopoietic progenitor cells G. Varady, O. Ujhelly, J. Cervenak, M. Grez, A. Varadi, B. Sarkadi, *K. Nemet - Budapest, Hungary

403

Reinjection of ex vivo expanded non-human primate bone marrow mononucleated cells (BMMNC) strongly reduces radiation-induced aplasia * J. M. Bertho, J. Frick, C. Demarquay, A. Chapel, N. Dudoignon, F. Trompier, J. Aigueperse, D. Thierry - Fontenay aux Roses, France

404

The role of different stromal support on the maintainance and expansion of haemopoietic progenitor cells M. Berger, *F. Fagioli, L. Gastaldo, C. Niceforo, K. Mareschi, E. Biasin, A. Palmero, R. Pessolano, E. Madon - Torino, Italy

405

Telomerase Activity as Early Detection of Preleukemia * L. R. Marsaman, T. Achmad, A. Maskoen - Bandung, Indonesia

406

BCR/ABL-mediated increased expression of multiple genes implicated in the pathogenesis of chronic myelogenous leukemia * S. Salesse, C. Verfaillie - Minneapolis, U.S.A.

407

The diagnosis of concealed hemoblastosis and minimal residual disease by means of original culture methods * T. Saralidze, T. Shvelidze, L. Mochevishvili, C. Zakareishvili, K. Rusishvili, P. Gabunia, A. Shengelaia - Tbilisi, Georgia

409

Cord Blood Banking and Transplants: Experience of a Single Center in China * Z. C. Han, L. Qiu, J. Han, Q. Li, C. Yang, M. Lu, H. Ying, M. Wang, M. Han - Tianjing, China

410

Abnormal expression of VEGF and its receptors in acute myeloid leukemia Y. Wang, Z. Xiao, R. Yang, *Z. C. Han - Tianjing, China

411

A novel factor capable of stimulating hematopoiesis and angiogenesis * Z. C. Han, Y. Liu, Y. Cai, S. Lu - Tianjing, China

412

Treatment of spinal injury by human umbilical cord blood stem cell transplantation * Z. C. Han, Z. Zhao, H. Li, H. Liu, R. Yang - Tianjing, China

335

The B-Cell Lymphoma Protein (BCL10) forms a large proapoptotic complex in the cytoplasm. * A. Ali-Saleh, C. Vijayasarathy - Al-Ain, United Arab Emirates

437

Definition of spontaneous (culture-induced) apoptosis in leukemic cells and its potential clinical relevance in childhood acute leukemia V. Shman, Valery P. Savitsky, Michael *P. Potapnev - , Minsk, Belarus

Program / Monday, July 8

16:30 - 18:00

34

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Monday, July 8, 2002 16:30 - 18:00

POSTER SESSION

52 Viruses

Program / Monday, July 8

413

Detection of Toxoplasma Gondii and Human Cytomegalovirus DNA in Blood from Transplant Recipients Using Multiplex Nested Polymerase Chain Reaction * N. Khodai Booran, V. Bakayev - Tehran, Iran

414

Dynamics of the cytomegalovirus - dendritic cell interaction:Dendritic cell maturation stage, major histocompatibility complex and viral gene functions control T cell activation S. Mathys, T. Schroeder, M. Messerle, U. Koszinowski, *U. Just - Muenchen, Germany

415

Platelet- and megakaryocyte-derived microparticles transfer CXCR4 receptor to CXCR4-null cells and make them susceptible to infection by X4-HIV T. Rozmyslowicz, M. Majka, J. Kijowski, G. Gaulton, *M. Ratajczak - Louisville, U.S.A.

416

Virus modulation effects on autoimmue idiopathic thrombocytopenia andanemia models in the mouse * A. Musaji, J.-P. Coutelier - Brussels, Belgium

417

Qualitative and quantitative analysis of human herpesviruses in chronic and acute B-cell lymphocytic leukemia and in multiple myeloma * S. Hermouet, C. Sutton, T. Rose, I. Corre, J. Casey - Nantes, France

418

Implication of hepatitis C virus (HCV) and human herpesvirus-8 (HHV-8) in the pathogenesis of a case of aggressive primary plasma cell leukemia (PCL) * S. Hermouet, I. Corre, M. Gassin, E. Bigot-Corbel, J. Casey - Nantes, France

419

Induction of Human Cytomegalovirus (CMV)-specific Cytotoxic T-lymphocytes (CTLs) by Artificial Antigen Presenting Cells (AAPC) * G. Papanicolaou, J. Latouche, M. Sadelain - New York, U.S.A.

420

Associated viral infections in patients with hematologic malignancies after BMT K. Abdulkadirov, *V. Tchebotkevitch, S. Moiseev - St. Petersburg, Russian Federation

421

Withdrawn

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

35

Scientific Program Tuesday, July 9, 2002 08:30 - 10:00

53 Presidential Symposium LE GRANDE SALON Chairs: Esmail D. Zanjani (Reno, NV, U.S.A), Connie J. Eaves (Vancouver, BC, Canada)

422

High gene transfer efficiency and pancellular erythroid expression resulting in correction of Anemia in ß-thalassemic mice with lentiviral-based human ß-globin vector * S. Imren, R. Pawliuk, B. Cavilla, C. Eaves, L. Wadsworth, R. Nagel, M. Fabry, E. Bouhassira, I. London, P. Leboulch, R. K. Humphries - Vancouver, Canada

423

Xenotransplant of Human Adipose-derived Mesenchymal Stem Cells * T. Meyerrose, D. A. DeUgarte, G. McNamara, M. H. Hedrick, J. A. Nolta - St. Louis, U.S.A.

424

Differentiative Potential Of Human Metanephric Mesenchymal Stem Cells * G. Almeida-Porada, Reno, U.S.A.

425

Ex-vivo transient expression system utilizing human erythroblasts for analysis of effects of transcriptional factors A. Di Baldassarre, M. Di Rico, A. Franco Migliaccio, *G. Migliaccio, H. Asano, Q. Li, G. Stamatoyannopoulos - Rome, Italy

426

Evi9 is a Zinc Finger Protein That is Required For Normal B and T Cell Development and May Function as a T-Cell Tumor Suppressor P. Liu, M. Ortiz, L. Tessarollo, T. Nakamura, N. Jenkins, N. Copeland, *J. Keller - Frederick, U.S.A.

10:00 - 12:00

Rac2 Regulates T and B Lymphocyte Chemotaxis, Distribution and Function * A. Roberts, B. Croker, E. Handman, D. Williams, D. Tarlinton - Parkville, Australia PLENARY SESSION

54 Gene Function Discovery and Transfection Chairs: Gerald B. Price (Montreal, QC, Canada), Hans Messner (Toronto, ON, Canada) 428

Hematopoietic Stem Cell Gene Transfer: Prospects for Therapeutic Gene Delivery * J. Tisdale - Bethesda, U.S.A.

429

Control of Hematopoietic Lineage: Molecular Mechanisms and Implications * S. Orkin - Boston, U.S.A.

430

Translational controlled expression of hematopoietic transcription factors * A. Leutz, E. Kowenz-Leutz, C. Müller, C. Calkhoven - Berlin, Germany

431

Leukemia Disease Genes: Prospects for High Throughput Gene Function Discovery in the Post Genome Era T. Suzuki, H. Shen, K. Akagi, P. Liu, T. Hisa, S. Spence, D. Du, H. Morse III, N. Jenkins, *N. Copeland - Frederick, U.S.A.

432

Genetic progression factors for murine acute promyelocytic leukemia M. Walter, J. Pollock, K. Oldfather, P. Westervelt, *T. Ley - St. Louis, U.S.A.

Program / Tuesday, July 9

427

36

31st Annual Meeting: ISEH / Experimental Hematology 30 (2002) 1-36

Scientific Program Tuesday, July 9, 2002 13:30 - 15:30

PLENARY SESSION

55 Cord Blood Transplantation

Program / Tuesday, July 9

Chairs: Peter Wernet (Duesseldorf, Germany), Catherine M. Verfaillie (Minneapolis, MN, U.S.A.) 433

Cord Blood Transplantation: Clinical Outcomes and Factors Affecting Prognosis * P. Rubinstein - New York, U.S.A.

434

Unrestricted Multipotential Stem Cells in Cord Blood: A Universal Donor Cell for Regenerative Medicine? * P. Wernet - Duesseldorf, Germany

435

Evaluation of Human Cord Blood HSC Engraftment In Vivo * E. Zanjani - Reno, U.S.A.

436

The New Stem Cell Biology: Clinical Implications * P. Quesenberry - Providence, U.S.A.