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Screening in the Dental Chair: A Multi-Center Study Pilot
OOOO Volume 122, Number 4 INSTITUTE, SEOUL NATIONAL UNIVERSITY, SOUTH KOREA (ROK). Objectives: The purpose of this study was to compare polymorphisms of the IL-1b and MUC7 genes between patients with burning mouth syndrome (BMS) and controls and to investigate relationships between these polymorphisms and clinical characteristics in BMS patients. Methods: Forty female BMS patients (62.2 10.0 years) and 40 gender- and age-matched controls (61.7 10.5 years) were included. Genomic DNA was extracted from saliva samples. Single nucleotide polymorphisms of IL-1b -511 and +3954 and variation in number of tandem repeats (VNTR) polymorphism of MUC7 were analyzed. Relationships between genotypic polymorphism data and clinical characteristics in BMS patients were also analyzed. Results: There were no significant differences in the genotypes of IL-1b -511 and +3954, and of MUC7 between the groups. There were no significant differences in symptom duration and intensity of BMS patients according to their IL-1b and MUC7 genotypes. The T allele of IL-1b -511 showed associations with psychometry results in BMS patients: paranoid ideation (P ¼ .014), global severity index (P ¼ .025), and positive symptom total (P ¼ .008). Conclusions: The genotypic polymorphisms of IL-1b -511 and +3954, and of MUC7 VNTR, had no direct associations with the development of BMS. However, the T allele of IL-1b -511 may increase the risk of BMS by increasing psychological asthenia.
SCREENING IN THE DENTAL CHAIR: A MULTI-CENTER STUDY PILOT SHU-YIN J. LEUNG, HOWARD LAVIGNE, MICHAEL HATTON, RONNIE MYERS, YANFANG REN, BENJAMIN TSOI, DARIA BOCCHER-LATTIMORE, MARA SAN ANTONIOGADDY, LYN STEVENS, LOU SMITH. NEW YORK STATE DEPARTMENT OF HEALTH, USA. Objectives: Since 2010, New York State (NYS) HIV Testing Law mandates the offering of HIV screening tests to patients ages 13-64 receiving hospital or primary care, but not dental care. We investigated the feasibility, acceptability, and outcome of HIV screening in three NYS academic dental centers (NYSADC), and assessed the proportion of patients who (1) accepted HIV testing when offered, and (2) tested HIV reactive. Methods: Between 2012 and 2014, free HIV tests were offered to patients at NYSADC dental clinics in NY City, Rochester, and Buffalo. The HIV OraQuick Advance Rapid TestTM was administered to patients accepting the offer. Patients with reactive results were offered confirmatory testing and linked to medical care. Patient demographics, primary care encounters in the previous year, and previous HIV testing experience were analyzed in a logistic regression model of test acceptance. Results: Of the 8570 patients seen, 54 (0.63%) were selfreported HIV positive, and 7869 presumed negative patients were offered HIV testing (91.8%), with 3982 (50.6%) accepting the test. Of those tested, five patients (0.13%) were confirmed HIV positive and linked to care. Logistic regression indicated the lack of primary care in the past year (AOR¼1.64, 95% CI 1.43-1.87) and no previous HIV test (AOR¼1.40, 95% CI 1.23-1.59) as significant predictors of test acceptance. Conclusions: Half of the patients seen accepted the test, a level consistent with existing literature. Of those, 0.13% were HIV positive. The positivity rate is above the 0.10% rate
ABSTRACTS Abstracts e113 recommended by CDC for routine screening, and supports continued testing in dental care settings. HIV screening in the dental chair is well accepted by patients, easy to implement, and affords an important opportunity for patients who are unaware of their HIV status or who have not seen a primary care provider in the previous year. A study involving community dental service providers is being developed.
DNA MICROARRAY ANALYSIS OF SUBMANDIBULAR GLANDS IN IGG4RELATED DISEASE MIHO OHTA, MASAFUMI MORIYAMA, TAKASHI MAEHARA, AKIHIKO TANAKA, SACHIKO FURUKAWA, KEIGO KUBOTA, MASAKI YAMAUCHI, NORIKO ISHIGURO, YURIE MIKAMI, SEIJI NAKAMURA. KYUSHU UNIVERSITY, JAPAN. Objectives: IgG4-related disease (IgG4-RD) is a novel systemic disease entity characterized by elevated serum IgG4 and tissue infiltration of IgG4-positive plasma cells accompanied by severe fibrosis. Although recent studies demonstrated that innate immune cells including monocytes and macrophages might promote local fibrosis and IgG4 production, the pathological mechanism remains unclear. Therefore, in this study, we sought to identify the disease-associated genes, especially innate immune molecules. Methods: Gene expression was analyzed by DNA microarray in submandibular glands (SMGs) from patients with IgG4-RD (n¼5), chronic sialoadenitis (CS) (n¼3), and controls (n¼3). Differentially expressed genes (DEGs) were validated by real-time PCR and immunohistochemical staining in IgG4-RD (n¼18), CS (n¼4), Sjögren’s syndrome (n¼11), and controls (n¼10). Results: Gene expression patterns in the three groups were quite different from each other by the pvclust method and principal components analysis. In IgG4-RD, 1,028 up-regulated genes and 692 down-regulated genes were identified as DEGs (P < .05). GO term analysis indicated that the up-regulated DEGs in IgG4-RD encoded proteins involved in T/B cell activation and chemotaxis. PCR validated significantly higher expression of macrophage receptor with collagenous structure (MARCO), a pattern-recognition receptor, in IgG4-RD compared with the other groups (P < .01). Immunohistochemical analysis confirmed that the expression pattern of MARCO was similar to that of the M2 macrophage marker CD163. Conclusions: MARCO was identified as a disease-associated molecule in IgG4-RD by DNA microarray. Moreover, M2 macrophages might contribute to the initiation of IgG4-RD via MARCO.
PENTOXIFYLLINE WITH VITAMIN E AND LASER THERAPY FOR MRONJ LEAH BOWERS, CHERRY ESTILO, SAEHEE YOM, NEAL LEHRMAN, JOSEPH HURYN. MEMORIAL SLOAN KETTERING CANCER CENTER, USA. Objectives: The aim of this study was to compare preliminary outcomes of patients with medication-related osteonecrosis of the jaw treated with either an oral regimen of pentoxifylline with vitamin E or low level laser therapy. We report on a case series of 8 patients with medication-related osteonecrosis of the jaw; 5 treated with pentoxifylline and vitamin E and 3 treated with low level laser therapy at Memorial Sloan Kettering Cancer Center in conjunction with Laser Periodontal Associates of New York between March of 2012 and the present day.
SCREENING IN THE DENTAL CHAIR: A MULTI-CENTER STUDY PILOT SHU-YIN J. LEUNG, HOWARD LAVIGNE, MICHAEL HATTON, RONNIE MYERS, YANFANG REN, BENJAMIN TSOI, DARIA BOCCHER-LATTIMORE, MARA SAN ANTONIOGADDY, LYN STEVENS, LOU SMITH. NEW YORK STATE DEPARTMENT OF HEALTH, USA. Objectives: Since 2010, New York State (NYS) HIV Testing Law mandates the offering of HIV screening tests to patients ages 13-64 receiving hospital or primary care, but not dental care. We investigated the feasibility, acceptability, and outcome of HIV screening in three NYS academic dental centers (NYSADC), and assessed the proportion of patients who (1) accepted HIV testing when offered, and (2) tested HIV reactive. Methods: Between 2012 and 2014, free HIV tests were offered to patients at NYSADC dental clinics in NY City, Rochester, and Buffalo. The HIV OraQuick Advance Rapid TestTM was administered to patients accepting the offer. Patients with reactive results were offered confirmatory testing and linked to medical care. Patient demographics, primary care encounters in the previous year, and previous HIV testing experience were analyzed in a logistic regression model of test acceptance. Results: Of the 8570 patients seen, 54 (0.63%) were selfreported HIV positive, and 7869 presumed negative patients were offered HIV testing (91.8%), with 3982 (50.6%) accepting the test. Of those tested, five patients (0.13%) were confirmed HIV positive and linked to care. Logistic regression indicated the lack of primary care in the past year (AOR¼1.64, 95% CI 1.43-1.87) and no previous HIV test (AOR¼1.40, 95% CI 1.23-1.59) as significant predictors of test acceptance. Conclusions: Half of the patients seen accepted the test, a level consistent with existing literature. Of those, 0.13% were HIV positive. The positivity rate is above the 0.10% rate
ABSTRACTS Abstracts e113 recommended by CDC for routine screening, and supports continued testing in dental care settings. HIV screening in the dental chair is well accepted by patients, easy to implement, and affords an important opportunity for patients who are unaware of their HIV status or who have not seen a primary care provider in the previous year. A study involving community dental service providers is being developed.
DNA MICROARRAY ANALYSIS OF SUBMANDIBULAR GLANDS IN IGG4RELATED DISEASE MIHO OHTA, MASAFUMI MORIYAMA, TAKASHI MAEHARA, AKIHIKO TANAKA, SACHIKO FURUKAWA, KEIGO KUBOTA, MASAKI YAMAUCHI, NORIKO ISHIGURO, YURIE MIKAMI, SEIJI NAKAMURA. KYUSHU UNIVERSITY, JAPAN. Objectives: IgG4-related disease (IgG4-RD) is a novel systemic disease entity characterized by elevated serum IgG4 and tissue infiltration of IgG4-positive plasma cells accompanied by severe fibrosis. Although recent studies demonstrated that innate immune cells including monocytes and macrophages might promote local fibrosis and IgG4 production, the pathological mechanism remains unclear. Therefore, in this study, we sought to identify the disease-associated genes, especially innate immune molecules. Methods: Gene expression was analyzed by DNA microarray in submandibular glands (SMGs) from patients with IgG4-RD (n¼5), chronic sialoadenitis (CS) (n¼3), and controls (n¼3). Differentially expressed genes (DEGs) were validated by real-time PCR and immunohistochemical staining in IgG4-RD (n¼18), CS (n¼4), Sjögren’s syndrome (n¼11), and controls (n¼10). Results: Gene expression patterns in the three groups were quite different from each other by the pvclust method and principal components analysis. In IgG4-RD, 1,028 up-regulated genes and 692 down-regulated genes were identified as DEGs (P < .05). GO term analysis indicated that the up-regulated DEGs in IgG4-RD encoded proteins involved in T/B cell activation and chemotaxis. PCR validated significantly higher expression of macrophage receptor with collagenous structure (MARCO), a pattern-recognition receptor, in IgG4-RD compared with the other groups (P < .01). Immunohistochemical analysis confirmed that the expression pattern of MARCO was similar to that of the M2 macrophage marker CD163. Conclusions: MARCO was identified as a disease-associated molecule in IgG4-RD by DNA microarray. Moreover, M2 macrophages might contribute to the initiation of IgG4-RD via MARCO.
PENTOXIFYLLINE WITH VITAMIN E AND LASER THERAPY FOR MRONJ LEAH BOWERS, CHERRY ESTILO, SAEHEE YOM, NEAL LEHRMAN, JOSEPH HURYN. MEMORIAL SLOAN KETTERING CANCER CENTER, USA. Objectives: The aim of this study was to compare preliminary outcomes of patients with medication-related osteonecrosis of the jaw treated with either an oral regimen of pentoxifylline with vitamin E or low level laser therapy. We report on a case series of 8 patients with medication-related osteonecrosis of the jaw; 5 treated with pentoxifylline and vitamin E and 3 treated with low level laser therapy at Memorial Sloan Kettering Cancer Center in conjunction with Laser Periodontal Associates of New York between March of 2012 and the present day.