- Email: [email protected]
Screening of natural herbs as mitigation agents for atopic symptoms
S444
Abstracts / Journal of Biotechnology 136S (2008) S402–S459
V4-P-110 Study of the atopic dermatitis improvement of placenta atopy cream
DMAPODA/SA vesicles and the stabilities and release properties of vesicles depended on the glucose concentration. In conclusion, the cationic vesicles incorporating GOd is thought to be used as drug delivery carriers for diabetes.
Seung-Hye Park 1 , Hong-Seon Lee 1 , In Soo Suh 2 , Ho-Sang Lee 2 , Chan-Hyun Jin 2 , Beom-jun Kim 1,∗
References
1 Nano Laboratory of Interlees, 293-1, Dongwon-Dong, Bundang-Gu, Seongman-Si, Gyeonggi-Do, Republic of Korea 2 Jeju Hi-Tech Industry Development Institute, 4-8 Ara-1-Dong, Jeju-Si, Jeju 690-121, Republic of Korea In general, a placenta is an organ rich in blood vessels that develops in female mammals during pregnancy and it contains full of hormones, so theoretically, they should improve the complexion. To evaluate the effect of placenta as a proper cosmetic ingredient, we focused on the emulsion for atopic dermatitis. Placenta atopy cream used in this study contains active ingredients extracted from the swine placenta originated from Jeju island in Korea. We instructed 12 patients with atopic dermatitis to apply cream to the lesions twice on whole body and often on affected parts for 8 weeks to determine the effectiveness and safety. The efficacy was evaluated by measuring EASI score by dermatology and electrical measurement of water holding capacity (corneometer), Tewameter. pH value was also measure by Skin pH meter. The placenta atopy cream was effective for atopic dermatitis improvement.
Department of Biological Engineering, Inha University, Incheon, Republic of Korea 2 Research Institute of MG Intobio Co., Ltd., Incheon, Republic of Korea
Keywords: Placenta; Atopic dermatitis; Moisturizer
E-mail address: [email protected] (E.-K. Kim).
doi:10.1016/j.jbiotec.2008.07.1030 V4-P-111 Preparation and characterization of glucose-sensitive vesicles incorporating glucose oxidase Xia Yang a,b , Seong-Min Jo a,b , Jae Hyung Choi a,b , Taek Kwan Kwon a,b , Mi Kyung Kang a,b , Jin-Chul Kim a,b,∗ a
School of Biotechnology & Bioengineering, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea b Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea Cationic vesicle incorporating Glucose oxidase (GOd) could generate a glucose-sensitive drug carrier in response to the variation of glucose concentration. This is because that glucose can be catalyzed to gluconic acid which decreased the pH of the solution (Hill et al., 1997). In that case (pH < 4.5), the salt bridging between NH3 + of N-[3-(dimethylamino)propyl]-octadecanamide (DMAPODA) and COO− of stearic acids (SA) was broken off causing the leakage of vesicles and released the drug as a result. In our study, DMAPODA/SA vesicles were prepared by hand-shaking method followed by sonication. The molar ratios of DMAPODA/SA were 1:1, 2:3, 3:2, 1:2, 2:1, 1:3 and 3:1. Calcein, a fluorescence marker for release properties investigation, was also loaded in vesicles during the hydrated process of vesicles preparation (Kim et al., 2002). The glucose-dependent release properties of Calcein-loaded vesicles were investigated on a fluorospectrophotometer at various glucose concentrations such as 0 mg/ml, 50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 300 mg/ml, 400 mg/ml. And the ranges of pH change by GOd catalyzed reaction and enzyme activities of free-enzyme and incorporated enzyme were also measured. On the other hand, the vesicles were characterized by transmission electron microscopy (TEM), zeta potentials and size measurements. According to the results, GOd was successfully incorporated with
Hill, K.J., Kaszuba, M., Creeth, J.E., Jones, M.N., 1997. Reactive liposomes encapsulating a glucose oxidase-peroxidase system with antibacterial activity. Biochim. Biophys. Acta 1326, 37–46. Kim, J.-C, Song, M.-E, Kim, M.-J, Lee, E.-J, Park, S.-K, Rang, M.-J, Ahn, H.-J., 2002. Preparation and characterization of Triclosan-containing vesicles. Colloids Surf. B Biointerf. 26, 235–241.
doi:10.1016/j.jbiotec.2008.07.1031 V4-P-112 Screening of natural herbs as mitigation agents for atopic symptoms Young-Bum Kim 1 , Jung-Hyun Kim 1 , Hyang-Bok Lee 1 , In Su Lee 2 , Kyoung Mo Ku 2 , Dong Joon Kim 2 , Eun-Ki Kim 1,∗ 1
Childhood atopic dermatitis shows considerable heterogeneity in its presentation and duration, as the cases, pruritus, inflammation, infection and immune hypersensitivity reaction may be symptoms of atopic dermatitis. Also genetic, microbial and environmental reasons can lead to atopy, but we focused on the symptoms of atopic dermatitis. Anti-oxidative activity was employed as screening of activated oxygen species scavenging agents. inhibition rate of nitric oxide and prostaglandin synthesis represented anti-pruritus and antiinflammatory activities, respectively, also immune response was associated with increased IgE levels. In order to develop natural herbs as mitigation agents for atopic symptoms, natural herbs that were showed anti-oxidative activities (DPPH assay) and cell viabilities (MTT assay) from Chinese plants were evaluated and selected by low cytotoxicity and high antioxidative activities, after that mitigation agents of atopic symptoms were selected in respect to inhibition rate of nitric oxide synthesis, inhibition rate of prostaglandin synthesis, immune suppressive activities and anti-microbial activities which were represented by sequential efficiency analysis and relative performance index. Five natural herbs were selected and designated as NC18, Miyong, LS-B31, NC15, MG-S and they showed commercial potentials as mitigation agents of atopic symptoms. References Lin, L.Y., Fisher, D.E., 2007. Melanocyte biology and skin pigmentation. Nature 445, 843–850. Emily, C., Nusrat, R., 2008. Functional characterization of the atopy-associated gene PHF11. J. Allergy Clin. Immunol.. Irum, S., Ajit, K.S., 2008. Immune up regulatory response of a non-caloric natural sweetener, stevioside. Chem. Biol. Interact..
doi:10.1016/j.jbiotec.2008.07.1032
Abstracts / Journal of Biotechnology 136S (2008) S402–S459
V4-P-110 Study of the atopic dermatitis improvement of placenta atopy cream
DMAPODA/SA vesicles and the stabilities and release properties of vesicles depended on the glucose concentration. In conclusion, the cationic vesicles incorporating GOd is thought to be used as drug delivery carriers for diabetes.
Seung-Hye Park 1 , Hong-Seon Lee 1 , In Soo Suh 2 , Ho-Sang Lee 2 , Chan-Hyun Jin 2 , Beom-jun Kim 1,∗
References
1 Nano Laboratory of Interlees, 293-1, Dongwon-Dong, Bundang-Gu, Seongman-Si, Gyeonggi-Do, Republic of Korea 2 Jeju Hi-Tech Industry Development Institute, 4-8 Ara-1-Dong, Jeju-Si, Jeju 690-121, Republic of Korea In general, a placenta is an organ rich in blood vessels that develops in female mammals during pregnancy and it contains full of hormones, so theoretically, they should improve the complexion. To evaluate the effect of placenta as a proper cosmetic ingredient, we focused on the emulsion for atopic dermatitis. Placenta atopy cream used in this study contains active ingredients extracted from the swine placenta originated from Jeju island in Korea. We instructed 12 patients with atopic dermatitis to apply cream to the lesions twice on whole body and often on affected parts for 8 weeks to determine the effectiveness and safety. The efficacy was evaluated by measuring EASI score by dermatology and electrical measurement of water holding capacity (corneometer), Tewameter. pH value was also measure by Skin pH meter. The placenta atopy cream was effective for atopic dermatitis improvement.
Department of Biological Engineering, Inha University, Incheon, Republic of Korea 2 Research Institute of MG Intobio Co., Ltd., Incheon, Republic of Korea
Keywords: Placenta; Atopic dermatitis; Moisturizer
E-mail address: [email protected] (E.-K. Kim).
doi:10.1016/j.jbiotec.2008.07.1030 V4-P-111 Preparation and characterization of glucose-sensitive vesicles incorporating glucose oxidase Xia Yang a,b , Seong-Min Jo a,b , Jae Hyung Choi a,b , Taek Kwan Kwon a,b , Mi Kyung Kang a,b , Jin-Chul Kim a,b,∗ a
School of Biotechnology & Bioengineering, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea b Institute of Bioscience and Biotechnology, Kangwon National University, 192-1, Hyoja 2-dong, Chunchon, Kangwon-do 200-701, Republic of Korea Cationic vesicle incorporating Glucose oxidase (GOd) could generate a glucose-sensitive drug carrier in response to the variation of glucose concentration. This is because that glucose can be catalyzed to gluconic acid which decreased the pH of the solution (Hill et al., 1997). In that case (pH < 4.5), the salt bridging between NH3 + of N-[3-(dimethylamino)propyl]-octadecanamide (DMAPODA) and COO− of stearic acids (SA) was broken off causing the leakage of vesicles and released the drug as a result. In our study, DMAPODA/SA vesicles were prepared by hand-shaking method followed by sonication. The molar ratios of DMAPODA/SA were 1:1, 2:3, 3:2, 1:2, 2:1, 1:3 and 3:1. Calcein, a fluorescence marker for release properties investigation, was also loaded in vesicles during the hydrated process of vesicles preparation (Kim et al., 2002). The glucose-dependent release properties of Calcein-loaded vesicles were investigated on a fluorospectrophotometer at various glucose concentrations such as 0 mg/ml, 50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg/ml, 300 mg/ml, 400 mg/ml. And the ranges of pH change by GOd catalyzed reaction and enzyme activities of free-enzyme and incorporated enzyme were also measured. On the other hand, the vesicles were characterized by transmission electron microscopy (TEM), zeta potentials and size measurements. According to the results, GOd was successfully incorporated with
Hill, K.J., Kaszuba, M., Creeth, J.E., Jones, M.N., 1997. Reactive liposomes encapsulating a glucose oxidase-peroxidase system with antibacterial activity. Biochim. Biophys. Acta 1326, 37–46. Kim, J.-C, Song, M.-E, Kim, M.-J, Lee, E.-J, Park, S.-K, Rang, M.-J, Ahn, H.-J., 2002. Preparation and characterization of Triclosan-containing vesicles. Colloids Surf. B Biointerf. 26, 235–241.
doi:10.1016/j.jbiotec.2008.07.1031 V4-P-112 Screening of natural herbs as mitigation agents for atopic symptoms Young-Bum Kim 1 , Jung-Hyun Kim 1 , Hyang-Bok Lee 1 , In Su Lee 2 , Kyoung Mo Ku 2 , Dong Joon Kim 2 , Eun-Ki Kim 1,∗ 1
Childhood atopic dermatitis shows considerable heterogeneity in its presentation and duration, as the cases, pruritus, inflammation, infection and immune hypersensitivity reaction may be symptoms of atopic dermatitis. Also genetic, microbial and environmental reasons can lead to atopy, but we focused on the symptoms of atopic dermatitis. Anti-oxidative activity was employed as screening of activated oxygen species scavenging agents. inhibition rate of nitric oxide and prostaglandin synthesis represented anti-pruritus and antiinflammatory activities, respectively, also immune response was associated with increased IgE levels. In order to develop natural herbs as mitigation agents for atopic symptoms, natural herbs that were showed anti-oxidative activities (DPPH assay) and cell viabilities (MTT assay) from Chinese plants were evaluated and selected by low cytotoxicity and high antioxidative activities, after that mitigation agents of atopic symptoms were selected in respect to inhibition rate of nitric oxide synthesis, inhibition rate of prostaglandin synthesis, immune suppressive activities and anti-microbial activities which were represented by sequential efficiency analysis and relative performance index. Five natural herbs were selected and designated as NC18, Miyong, LS-B31, NC15, MG-S and they showed commercial potentials as mitigation agents of atopic symptoms. References Lin, L.Y., Fisher, D.E., 2007. Melanocyte biology and skin pigmentation. Nature 445, 843–850. Emily, C., Nusrat, R., 2008. Functional characterization of the atopy-associated gene PHF11. J. Allergy Clin. Immunol.. Irum, S., Ajit, K.S., 2008. Immune up regulatory response of a non-caloric natural sweetener, stevioside. Chem. Biol. Interact..
doi:10.1016/j.jbiotec.2008.07.1032