- Email: [email protected]
Screening the urine of male adolescents for chlamydia trachomatis and neisseria gonorrhoeae with a DNA-probe test
66
ABSTRACT5OFPOSTERSPRESENTED
JOURNALOFADOLESCENTHEALTHVol.13,No.1
Development of a Screening Instrument for Low Calcium and High Fat Diets Among Adolescents. Howard Schubiner, M.D.; Paula Zemel, Ph.D. Wayne State University, Detroit, MI and University of Tennessee, Knoxville, TN. Nutritional histories are infrequently completed by primary care providers, can be time consuming to perform, and may not identify those who can benefit from intervention. The goal of this study was to develop a brief, self-report instrument to identify adolescents with low calcium and/or high fat diets. A convenience sample of 129 adolescents between 14 and 18 years (mean age = 16, 58% female) was recruited from four suburban high schools. They completed a survey of 23 questions developed to screen for calcium and fat related dietary patterns and a semiquantitative food frequency questionnaire. Average nutrient intakes determined by the food frequency questionnaire are as follows: calories = 2417 t/- 927, calcium = 1000 t/- 573 mg, fat - 107 t/- 84 gm, % calories from fat = 37 +/- 7%. The screening questions were correlated with calcium intake and % calories from Four questions concerning calcium-related dietary patterns fat. and five questions concerning fat-related dietary patterns had Pearson r values of at least 0.20 and comprised the screening tool. The cut-points of the screening instruments to detect adolescents with very low calcium (< 600 mg) or very high fat (> 40% calories from fat) intake were determined using receiver operating characteristic curves. Test characteristics were as follows: sensitivity - 74%, 72%; specificity - 79%, 59%; positive predictive value - 56%. 56% and negative predictive value - 89%, 75%; for calcium and % calories from fat, respectively. These nine screening questions can be used by primary care providers to correctly identify two-thirds to threequarters of adolescents with two targeted dietary risk factors.
Screening the Urine of Male Adolescents for Chlamydia Trachomatis and Neisseria Gonorrhoeae with a DNA-Probe Test. Howard Schubiner, M.D.; William LeBar; Claudia Jemal Wayne State University, Detroit, MI and Providence Hospital, Southfield, MI. At present, detection of Chlamydia trachomatis (Ct) and Neisseria gonorrhoeae (GC) in urine samples of males is not highly accurate. In an ongoing study at a public health department sexually transmitted disease clinic, urethral cultures for GC and Ct are being compared to a chemi-luminescent DNA-probe test (GenProbe Pace 2). Of the 106 males who participated ages 15-25 (x = 20.71, 76% had a urethral discharge. The prevalence of Ct was 19% and of GC was 40%. Six patients had both infections, The test characteristics of the DNA probe were : sensitivity - 90%, 95%; specificity - lOO%, 100%; positive predictive value - lOO%, 100%; negative predictive value - 98%, 97%; for Ct and GC, respectively. There were two false negative DNA probes for Ct and for GC while there were no false positives. If confirmed by further studies, this test provides a highly accurate method for the non-invasive diagnosis of chlamydial and gonoccocal urethritis in males.
ABSTRACT5OFPOSTERSPRESENTED
JOURNALOFADOLESCENTHEALTHVol.13,No.1
Development of a Screening Instrument for Low Calcium and High Fat Diets Among Adolescents. Howard Schubiner, M.D.; Paula Zemel, Ph.D. Wayne State University, Detroit, MI and University of Tennessee, Knoxville, TN. Nutritional histories are infrequently completed by primary care providers, can be time consuming to perform, and may not identify those who can benefit from intervention. The goal of this study was to develop a brief, self-report instrument to identify adolescents with low calcium and/or high fat diets. A convenience sample of 129 adolescents between 14 and 18 years (mean age = 16, 58% female) was recruited from four suburban high schools. They completed a survey of 23 questions developed to screen for calcium and fat related dietary patterns and a semiquantitative food frequency questionnaire. Average nutrient intakes determined by the food frequency questionnaire are as follows: calories = 2417 t/- 927, calcium = 1000 t/- 573 mg, fat - 107 t/- 84 gm, % calories from fat = 37 +/- 7%. The screening questions were correlated with calcium intake and % calories from Four questions concerning calcium-related dietary patterns fat. and five questions concerning fat-related dietary patterns had Pearson r values of at least 0.20 and comprised the screening tool. The cut-points of the screening instruments to detect adolescents with very low calcium (< 600 mg) or very high fat (> 40% calories from fat) intake were determined using receiver operating characteristic curves. Test characteristics were as follows: sensitivity - 74%, 72%; specificity - 79%, 59%; positive predictive value - 56%. 56% and negative predictive value - 89%, 75%; for calcium and % calories from fat, respectively. These nine screening questions can be used by primary care providers to correctly identify two-thirds to threequarters of adolescents with two targeted dietary risk factors.
Screening the Urine of Male Adolescents for Chlamydia Trachomatis and Neisseria Gonorrhoeae with a DNA-Probe Test. Howard Schubiner, M.D.; William LeBar; Claudia Jemal Wayne State University, Detroit, MI and Providence Hospital, Southfield, MI. At present, detection of Chlamydia trachomatis (Ct) and Neisseria gonorrhoeae (GC) in urine samples of males is not highly accurate. In an ongoing study at a public health department sexually transmitted disease clinic, urethral cultures for GC and Ct are being compared to a chemi-luminescent DNA-probe test (GenProbe Pace 2). Of the 106 males who participated ages 15-25 (x = 20.71, 76% had a urethral discharge. The prevalence of Ct was 19% and of GC was 40%. Six patients had both infections, The test characteristics of the DNA probe were : sensitivity - 90%, 95%; specificity - lOO%, 100%; positive predictive value - lOO%, 100%; negative predictive value - 98%, 97%; for Ct and GC, respectively. There were two false negative DNA probes for Ct and for GC while there were no false positives. If confirmed by further studies, this test provides a highly accurate method for the non-invasive diagnosis of chlamydial and gonoccocal urethritis in males.