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‘Sealing’ alters capillary permeability
419
Abstracts
synthetic rates. The same animals showed a 22 per cent decrease in per cent flux oxidized and a 7 per cent decrease in total energy expenditure compared with safflower oil-fed animals. A 15 per cent increase in liver protein fractional synthetic rate was accompanied by a significant elevation in total liver protein with MCT/fish oil administration. This latter lipid mixture reduced net protein catabolism following bums, in part by influencing tissue protein synthetic rates. The reduction in total energy expenditure is a unique feature of the MCT/fish oil mixture. Teo T. C., de Michele S. J., Selleck K. M. et al. (1989) Administration of structured lipid composed of MCT and fish oil reduces net protein catabolism in enterally fed burned rats. Ann. Surg. 210, (l), 100-107.
LABORATORY
STUDIES
‘Sealing’ alters capillary permeability Measurements were made of albumin leakage from standardized scalds inflicted on the rat jejunum with and without variously sized fractions of hydroxethyl starch (HES) admixed with the albumin to act as a sealing agent of the capillary pores. The HES fraction with molecular weights ranging between 100 000 and 300 000 daltons performed significantly better as a sealing agent than HES frac-
tions having molecular weights of 300 000 to 3.4 million daltons and with molecular weights of less than 50 000 daltons. Control groups received either Ringer’s lactate or 5 per cent albumin. AS the capillary sealing was shown to be independent of a colloid osmotic pressure effect it is believed to be related to the size and shape of the non-charged macromolecules. Zikria B. A ., King T. C., Stanford J. et al. (1989) A biophysical approach to capillary permeability. Surgery 105, (5). 625-631. Rapid estimate of biopsy bacterial content A rapid estimate of the extent of bacterial contamination in burn wound biopsy specimens can be obtained within 4 h of biopsy collection using a rapid manual histological technique (which allowed a classification of depth of bacterial involvement) and acridine orange fluorescent staining (for a semi-quantitative evaluation of the degree of infection). The results of these two techniques enabled rapid therapeutic decisions to be made while waiting for quantitative culture and sensitivity test results which arrive 24-48 h later. Mitchell V., Galizia J. P. and Foumier L. (1989) Precise diagnosis of infection in bum would biopsy specimens: combination of histologic technique, acridine orange staining and culture. J’.Bum cure Rehabil. 10,(3), 195-202.
Plastic Surgeon The Department Of Surgery at the University of Alberta is seeking a plastic surgeon who is willing to commit a significant portion of time to the medical and surgical care of patients in the new Firefighter’s Burn Treatment Unit in addition to surgical practice. Clinical or basic research experience is essential. Full university appointment at the assistant professor level available for a suitable candidate. Application deadline: 30 April, 1990. In accordance with Canadian immigration requirements priority will be given to Canadian citizens and permanent residents. Apply in writing with accompanying curriculum vitae to: Dr. C. 1. Lobay, Room 240,11044 82 Avenue, Edmonton, AB, Canada T6G OTZ. The University of Alberta is committed to the principle of equity in employment.
Abstracts
synthetic rates. The same animals showed a 22 per cent decrease in per cent flux oxidized and a 7 per cent decrease in total energy expenditure compared with safflower oil-fed animals. A 15 per cent increase in liver protein fractional synthetic rate was accompanied by a significant elevation in total liver protein with MCT/fish oil administration. This latter lipid mixture reduced net protein catabolism following bums, in part by influencing tissue protein synthetic rates. The reduction in total energy expenditure is a unique feature of the MCT/fish oil mixture. Teo T. C., de Michele S. J., Selleck K. M. et al. (1989) Administration of structured lipid composed of MCT and fish oil reduces net protein catabolism in enterally fed burned rats. Ann. Surg. 210, (l), 100-107.
LABORATORY
STUDIES
‘Sealing’ alters capillary permeability Measurements were made of albumin leakage from standardized scalds inflicted on the rat jejunum with and without variously sized fractions of hydroxethyl starch (HES) admixed with the albumin to act as a sealing agent of the capillary pores. The HES fraction with molecular weights ranging between 100 000 and 300 000 daltons performed significantly better as a sealing agent than HES frac-
tions having molecular weights of 300 000 to 3.4 million daltons and with molecular weights of less than 50 000 daltons. Control groups received either Ringer’s lactate or 5 per cent albumin. AS the capillary sealing was shown to be independent of a colloid osmotic pressure effect it is believed to be related to the size and shape of the non-charged macromolecules. Zikria B. A ., King T. C., Stanford J. et al. (1989) A biophysical approach to capillary permeability. Surgery 105, (5). 625-631. Rapid estimate of biopsy bacterial content A rapid estimate of the extent of bacterial contamination in burn wound biopsy specimens can be obtained within 4 h of biopsy collection using a rapid manual histological technique (which allowed a classification of depth of bacterial involvement) and acridine orange fluorescent staining (for a semi-quantitative evaluation of the degree of infection). The results of these two techniques enabled rapid therapeutic decisions to be made while waiting for quantitative culture and sensitivity test results which arrive 24-48 h later. Mitchell V., Galizia J. P. and Foumier L. (1989) Precise diagnosis of infection in bum would biopsy specimens: combination of histologic technique, acridine orange staining and culture. J’.Bum cure Rehabil. 10,(3), 195-202.
Plastic Surgeon The Department Of Surgery at the University of Alberta is seeking a plastic surgeon who is willing to commit a significant portion of time to the medical and surgical care of patients in the new Firefighter’s Burn Treatment Unit in addition to surgical practice. Clinical or basic research experience is essential. Full university appointment at the assistant professor level available for a suitable candidate. Application deadline: 30 April, 1990. In accordance with Canadian immigration requirements priority will be given to Canadian citizens and permanent residents. Apply in writing with accompanying curriculum vitae to: Dr. C. 1. Lobay, Room 240,11044 82 Avenue, Edmonton, AB, Canada T6G OTZ. The University of Alberta is committed to the principle of equity in employment.