Secretion of bioactive and immunoreactive interleukin-1 alpha (IL-1α) by human seminiferous tubules in vitro

P 180 SECRETICN OF BIOACTIVE AND IMMDNORFACTIi~ ~ H[P~N S~MI~'m~XJS %W3BULES IN VITRO

133 -

I

ALPHA (IL-1~) BY

S.A. KHAN & E. NIESCHIAG, Institute of Reproductive Medicine, University of M~nster, Steinfurter Str. 107, 4400 MUnster, Federal Republic of Germany The presence of an IL-1 like factor in rat and human testes and in htm~n ovarian follicular fluid has recently been reported. The present study was carried out to invest/gate the cellular source of this factor in human testis. Testes obtained frsm ~o~t~t~ cancer patients undergoing o r c h i d e c ~ w~re used to prepare whole tissue cytosols or to isolate various cell types. Interstitial cells (laydig cells and macrophages), peritubulav (myoid) cells a n d semihiferous tubules were either used to prepare fresh cytosols or were cultured for 24-48 h. The cytosols and the culture media were then analyzed for IL-I bioactivity using murine thymocyte proliferation bioassay. Interstitial cells and peritubular cells contained no IL-I activity nor did they secrete IL-I in culture. Cytosols prepared frcm whole testicular tissue or freshly isolated seminiferous tubules ccmta/ned high ~,ounts of IL-I bioactivity. MDreover, tubular pieces secreted significant amKx~ts of IL-I in vitro. IL-I frQm both testes cytosols and tubular culture media was eluted in molecular weight region between 10 kD and 30 kD after gel ~ t o g r a p h y . The IL-I bioactivity in all cases was associated with immunoreactive IL-I~ d e ~ by a specific ~ s a y (Amersham). It is concluded that hunan testes produce IL-I~ which is secreted by seminiferous tubules (Sertoli cells or germ cells) and does not originate in the testicular macrophages which are localized in the interstitial tissue. Keywords:

Interleukin-1 alpha, htm~n testis, s~miniferous tubules

P 181 SPECIFITY

OF

DIFFERENT

NATURE

METHODS

FOR

REVEALED OP SENSITIZATION

ANTIGENS

OF

A.A.KOCHETKOV, A.I.ABILOV, T.A. TAG Labbratory Reproductive Immunology of Animal Husbandry I42023, Strelkovo, Podolsky region, Moscow, USSR Autosensitization secrets

(ASGS)

of males

development.

Specifity

mation

and rosetten

(RBTL)

for revealed E-RFC

has been

Because

of rabbits

nonspeciflc

methods

glands

blasttr,n~for-

ASGS,

(S-RFC) seminal

R B T L has been specifity

antlgeneic

ASGS only

for anti-ASGS specifity,

antigenic

groups

for all types of immunization. for immuni-

(p
for revealed

testicular-epididymal

and nonsensibility Keywords:

against

for any nature

sensibility

only has been specific

against

E-RPC,

test w i t h autospermatozoas

of love sensibility

S-RPC males

sex

sperms was compared.

(p
zation against more

accessory

Institute

to semen quality and e m b r y o n i c

of three methods:

sensitization

plasma or epididymal

and equally

against

is not r e l a t i o n

AGAINST

EJACULATES

nature

immunization antigens

immunization.

sensitization,

seminal

of

(p
antigens.