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Segregation of three corynosoma spp. (acanthocephala) in paratenic hosts?
Poster Sessions / Parasitology
304
International 47 (Suppf.) (1998) 283-389
IN VITRO
P-0678
P-0676
CULTIVATION
OF ANGIOSTRON-
GYLUS COSTARICEh’SIS SEGREGATION OF THREE CORYNOSOMA SPP ( ACAh’THOCEPHALA) IN PARAENIC HOSTS?
H&J
tI,
Niimura M,
Department Tasnd E.T Valto”e”. Dep;lNnent of Biolo8icsl and Environmental JyxU&, Jyv8skyla. Finland
Science, University
of
Three Co~osoma species, C. scmerme, C.smrmosvm and C.magdalenr. were found ia young grey seals from the sot&em part of the Golf of Both&, Baltic sea. Suprisingly, the parasite population of 60”~ young seals was domiruted by C. magdolenr, while in others C. smmmsum predomi”ated All con-t i&ctio”s of c. selnerme. Oar int&tedseaIsalsoharboo& question is, how do these dlssimilsr pattans of se8regation develop in different seal specimens. We would expect that C.snvmosum and C
magdolenr arc also ~egngakd in the pmtenic host (a fish), becaw the trasmhion from the amphipodMonoporeiaa#nis ( htmncdhtc host) to seals ham only via a paratenic host. We have smdied this in sculpin from the Both&m Bay, notlheasem Gulf ofBotlmia We also studied the stmctue of Corynosomo populatio”s in the hosts. The remIt wore also compared with a” identical st”dy oarried out 17 years previously i” the ss”K srca. ReaIts: I” the most comma parptenic host for Covnosomu Mvorhcephalus qua&icornrs, C. xmermc ittfectio” in 1996-1997 was * the same love1 as 17 years earlier (82% and 83Y$ respeniwly). In the later period the infection of C. smrmosum-like species (C magdaleni and C.stnrmosum) was 13% whenas17yearscarlierit~been21%lothelattercase,tbctwospcdes were not separated. Amang the C. shrmosum-like specimtns identifcd in 1996-1997, 71% were C. magdaleni. Ihe local segregation behveen C. ~trumowm and Cmagdaieni was indicated by the lack of C. sfnmwsum in sctdpincsaghtfromtbe coastalarea(depthllm).Inthedeqxrarea(de@ 451”) both species were found, but not in the same fish spximeos. C. semerme populations exhibit a ntsIe:femaIe sex ratio of 1:1 in the summa months, wheress in October the munber of males wss higher (1:0.77X Also, the ovaries of C. sernernre females had io most case5 disintegrated into ovarian bsus (80%). co”clulo”: The prese”t resolts give an i”dicati0” of hut do not confirm the segregario” of C. magdalem and C. shwmoswn in their
paratenic hmts.
Inohsna,
Aosai
F,
Uluversity
It is known
that
a culture
defined
medium
will
for various
studies
chemically condition
larvae of A. eosticensis
chemreally
defined
nutitional
requirements
components argimne,
medium
Yano
A
School of Medicine,
L-phenylalanme,
examined
medium.
Ltbmonine,
choline chloride and pyridoxitx
also required
essential
examtned. required
for their development. components
Llysme. and
higher
components. amino
to idenhfy
mammalran
the
parasitic
were also successfully
nematodes.
Opbmum
cultured
Glucosewas
concentranons
of
development
were
and tryptophan
were
for worm
essential
Furthermore,
were
Among the 11
Toour knowledge,
acids
L-
a “ucle~c acid precursor,
concentrations
study
L-valme,
were essential.
for the parasite’s
than the other essential
namely
L-methlonme,
development.
It was shown that chohne chlonde at relatively
cultured
I” Waymouth’s
Ten amino aads.
by the worms, but hypoxanthme,
was not necessary
development this is the tirst
for development A. costarice”&
of eggs
to the first stage larvae I” chemically
defined Ham’s F- 12 medium. Ten days afiercultivation
in this medium,
34% ofthe eggs developed to tirst stage larvae. When these larvae were mfectsd to the fist
mtermedlate
host snails,
Biomphalaria
glabrata,
into third stage larvae.
P-0679 AND PARASITOLOGlCAL HYDROBIOLOGICAL CHARACTERISTICS OF RESERVOIRS OF “KOSEWO GURNE” BIOLOGICAL STATIONS
mM.* and Wits I.**. lI”stihae of Hydmbiology National .&a&my of Sciences. Kyi\,. Ukraine. **Institute of Parssitologv Polish Academy of Sciences. Warsaw, Poland. The “Kosewo-Gume” biological statson 1s located in NORTHEAST of Poland The flowless Lake “Kuc” and “urnher of small water bodies are pIaced on ns territory The basical psrt of water balance of reservoirs nuke aa&@mmd soaxes ard s&ace drain The small-sized water lx&es are ldrared into a zone of habitat of &en $ number more than 100 speciments. 0” “lain hydrobiologicsl parametcn they are referred to poly- and hypertmphical classes (Oxiyuk. Zhulrynski. 1994). The larvae of Diptera, molhzs and amphibian are predominated there The Tetracofvl~doe. Echtnostomatidae, Diplostomatidae. and Gorgoderidae trematodes are dominated in the fauna of parasites.The percent of infected invertebrates ranglcd from 14.0 % (B~va~vra) up to 58.3% (Ga~lropodo). The highen pacerd of infection among the puasites having no intennediste hosts (micmsporidia and mermitid wornxs) was mrde-d in the temporary resu&rs with the high level of orsanjc pollution. More than 5 O/oof Daphnio kmgispina ue infected by microsporidium Perezia Sp Tlu area of water-surface of lake Kuz is more than 10.0 sq. Kms. The depth and transparence were reached 30.0 m, and 4.0 m. consequently. Aquatic rnacrophytes covered about 10% of their surface. Mostly Perca flwioh’lis, Esox lucius and Rut&~ rurrlus are represented among the fishes. The copepOda, chironmnid larvae. snails and DreissPna’s bivalves were composed the most of species cd pIankton and benthos. The Lake Kuc is retkrmd to mesotrophicaI class Connected with fishes, and birds tremstodes (Diptostomatidoe, Microphaltidae. Echmostomat~dae) were fomxl there fWmnns and Acanrhocephdlidae are rewrded Th Triaenophorus w. The panvjtes with the simple life cycles rue represented by &gai//idw and .@xobo/idae and Gregurinrdw species The percent of i&cted invertebrate and vertcbrale hosts ranged from 3.5% up to 36.4 % comapen@
a
In the present study, by delehon of single
L-byptophan,
for the parasite’s
using controlled
We successfully
to young adults
L~~soleucme, L-leucine,
shown to be essential vitamins,
for paxasltes
a reasonably
MB 75111. were
from Waymouth’s
L-hlstldme,
system
provide
on parasites.
third stags
these
M,
Chiba
Chiba City, Japan
they developed
P-0677
Kobayashi
of Parasitology,
MODIFICATED BAERMAN METHOD. For ohtention of third s&x lmvae of-1~ Costsn&o& from slu8s L&moM.*,LazoR* Research Center OfParasite snd Fungus Diseasu.CIDRALAS Guyaquil,Ea&or I” 1983 has heen described in Ecudor the “A”gioslron@s wstarriccnois”(Fiist record of Artgiosuo”gylus cosunicencir in Eeusdor.The American Journal of Tropical Medicine pod Hygicoe Vol. 32) We real&d a research work “mltidisciplii with R. Lam as the investigating managa with the advising of P. h&rera, atxl in IS90 it’s bee” publicated “Abdominal mlus in Ecuador”. We awlv the modificated baumao method to fotmd third staae larvae of tbc family. I” our&sear&&rtcr we add as a rutinuy diagnostic this mahod for the ohtention of Stmngyloides stexc&ris larvae in faecea with a hi&r _. positivism. In a urban tighhorhood in the &west of Guayaquil @c&or) we have found several of this moll~s in the aroud of a child oarkwe wninsted this material with the pourpose to know iFthey wem infect& WeNiscentedtheslu~sndthenweliquated.thenintroducedthcproducr imoaaplasticconlainer,sulwithagauu:.andt~tumovaaodpdinfoa c4mieglass,thstco~pmifyingwstcrat40~C A&r 30 mioutes we pick up with a pipette, oae goutof the &em md we take it to the object port to observed with a Iiile msgnification, and we found seversI motive larvae, we dye with lug01 sod identificate. of 84 molluscs that we attalized 31 were similar tha” the third sta8e larvae of the -odes An8iostrongylus costuricmcis. This method its easy, and have a faster elaboration with a higher positivism
304
International 47 (Suppf.) (1998) 283-389
IN VITRO
P-0678
P-0676
CULTIVATION
OF ANGIOSTRON-
GYLUS COSTARICEh’SIS SEGREGATION OF THREE CORYNOSOMA SPP ( ACAh’THOCEPHALA) IN PARAENIC HOSTS?
H&J
tI,
Niimura M,
Department Tasnd E.T Valto”e”. Dep;lNnent of Biolo8icsl and Environmental JyxU&, Jyv8skyla. Finland
Science, University
of
Three Co~osoma species, C. scmerme, C.smrmosvm and C.magdalenr. were found ia young grey seals from the sot&em part of the Golf of Both&, Baltic sea. Suprisingly, the parasite population of 60”~ young seals was domiruted by C. magdolenr, while in others C. smmmsum predomi”ated All con-t i&ctio”s of c. selnerme. Oar int&tedseaIsalsoharboo& question is, how do these dlssimilsr pattans of se8regation develop in different seal specimens. We would expect that C.snvmosum and C
magdolenr arc also ~egngakd in the pmtenic host (a fish), becaw the trasmhion from the amphipodMonoporeiaa#nis ( htmncdhtc host) to seals ham only via a paratenic host. We have smdied this in sculpin from the Both&m Bay, notlheasem Gulf ofBotlmia We also studied the stmctue of Corynosomo populatio”s in the hosts. The remIt wore also compared with a” identical st”dy oarried out 17 years previously i” the ss”K srca. ReaIts: I” the most comma parptenic host for Covnosomu Mvorhcephalus qua&icornrs, C. xmermc ittfectio” in 1996-1997 was * the same love1 as 17 years earlier (82% and 83Y$ respeniwly). In the later period the infection of C. smrmosum-like species (C magdaleni and C.stnrmosum) was 13% whenas17yearscarlierit~been21%lothelattercase,tbctwospcdes were not separated. Amang the C. shrmosum-like specimtns identifcd in 1996-1997, 71% were C. magdaleni. Ihe local segregation behveen C. ~trumowm and Cmagdaieni was indicated by the lack of C. sfnmwsum in sctdpincsaghtfromtbe coastalarea(depthllm).Inthedeqxrarea(de@ 451”) both species were found, but not in the same fish spximeos. C. semerme populations exhibit a ntsIe:femaIe sex ratio of 1:1 in the summa months, wheress in October the munber of males wss higher (1:0.77X Also, the ovaries of C. sernernre females had io most case5 disintegrated into ovarian bsus (80%). co”clulo”: The prese”t resolts give an i”dicati0” of hut do not confirm the segregario” of C. magdalem and C. shwmoswn in their
paratenic hmts.
Inohsna,
Aosai
F,
Uluversity
It is known
that
a culture
defined
medium
will
for various
studies
chemically condition
larvae of A. eosticensis
chemreally
defined
nutitional
requirements
components argimne,
medium
Yano
A
School of Medicine,
L-phenylalanme,
examined
medium.
Ltbmonine,
choline chloride and pyridoxitx
also required
essential
examtned. required
for their development. components
Llysme. and
higher
components. amino
to idenhfy
mammalran
the
parasitic
were also successfully
nematodes.
Opbmum
cultured
Glucosewas
concentranons
of
development
were
and tryptophan
were
for worm
essential
Furthermore,
were
Among the 11
Toour knowledge,
acids
L-
a “ucle~c acid precursor,
concentrations
study
L-valme,
were essential.
for the parasite’s
than the other essential
namely
L-methlonme,
development.
It was shown that chohne chlonde at relatively
cultured
I” Waymouth’s
Ten amino aads.
by the worms, but hypoxanthme,
was not necessary
development this is the tirst
for development A. costarice”&
of eggs
to the first stage larvae I” chemically
defined Ham’s F- 12 medium. Ten days afiercultivation
in this medium,
34% ofthe eggs developed to tirst stage larvae. When these larvae were mfectsd to the fist
mtermedlate
host snails,
Biomphalaria
glabrata,
into third stage larvae.
P-0679 AND PARASITOLOGlCAL HYDROBIOLOGICAL CHARACTERISTICS OF RESERVOIRS OF “KOSEWO GURNE” BIOLOGICAL STATIONS
mM.* and Wits I.**. lI”stihae of Hydmbiology National .&a&my of Sciences. Kyi\,. Ukraine. **Institute of Parssitologv Polish Academy of Sciences. Warsaw, Poland. The “Kosewo-Gume” biological statson 1s located in NORTHEAST of Poland The flowless Lake “Kuc” and “urnher of small water bodies are pIaced on ns territory The basical psrt of water balance of reservoirs nuke aa&@mmd soaxes ard s&ace drain The small-sized water lx&es are ldrared into a zone of habitat of &en $ number more than 100 speciments. 0” “lain hydrobiologicsl parametcn they are referred to poly- and hypertmphical classes (Oxiyuk. Zhulrynski. 1994). The larvae of Diptera, molhzs and amphibian are predominated there The Tetracofvl~doe. Echtnostomatidae, Diplostomatidae. and Gorgoderidae trematodes are dominated in the fauna of parasites.The percent of infected invertebrates ranglcd from 14.0 % (B~va~vra) up to 58.3% (Ga~lropodo). The highen pacerd of infection among the puasites having no intennediste hosts (micmsporidia and mermitid wornxs) was mrde-d in the temporary resu&rs with the high level of orsanjc pollution. More than 5 O/oof Daphnio kmgispina ue infected by microsporidium Perezia Sp Tlu area of water-surface of lake Kuz is more than 10.0 sq. Kms. The depth and transparence were reached 30.0 m, and 4.0 m. consequently. Aquatic rnacrophytes covered about 10% of their surface. Mostly Perca flwioh’lis, Esox lucius and Rut&~ rurrlus are represented among the fishes. The copepOda, chironmnid larvae. snails and DreissPna’s bivalves were composed the most of species cd pIankton and benthos. The Lake Kuc is retkrmd to mesotrophicaI class Connected with fishes, and birds tremstodes (Diptostomatidoe, Microphaltidae. Echmostomat~dae) were fomxl there fWmnns and Acanrhocephdlidae are rewrded Th Triaenophorus w. The panvjtes with the simple life cycles rue represented by &gai//idw and .@xobo/idae and Gregurinrdw species The percent of i&cted invertebrate and vertcbrale hosts ranged from 3.5% up to 36.4 % comapen@
a
In the present study, by delehon of single
L-byptophan,
for the parasite’s
using controlled
We successfully
to young adults
L~~soleucme, L-leucine,
shown to be essential vitamins,
for paxasltes
a reasonably
MB 75111. were
from Waymouth’s
L-hlstldme,
system
provide
on parasites.
third stags
these
M,
Chiba
Chiba City, Japan
they developed
P-0677
Kobayashi
of Parasitology,
MODIFICATED BAERMAN METHOD. For ohtention of third s&x lmvae of-1~ Costsn&o& from slu8s L&moM.*,LazoR* Research Center OfParasite snd Fungus Diseasu.CIDRALAS Guyaquil,Ea&or I” 1983 has heen described in Ecudor the “A”gioslron@s wstarriccnois”(Fiist record of Artgiosuo”gylus cosunicencir in Eeusdor.The American Journal of Tropical Medicine pod Hygicoe Vol. 32) We real&d a research work “mltidisciplii with R. Lam as the investigating managa with the advising of P. h&rera, atxl in IS90 it’s bee” publicated “Abdominal mlus in Ecuador”. We awlv the modificated baumao method to fotmd third staae larvae of tbc family. I” our&sear&&rtcr we add as a rutinuy diagnostic this mahod for the ohtention of Stmngyloides stexc&ris larvae in faecea with a hi&r _. positivism. In a urban tighhorhood in the &west of Guayaquil @c&or) we have found several of this moll~s in the aroud of a child oarkwe wninsted this material with the pourpose to know iFthey wem infect& WeNiscentedtheslu~sndthenweliquated.thenintroducedthcproducr imoaaplasticconlainer,sulwithagauu:.andt~tumovaaodpdinfoa c4mieglass,thstco~pmifyingwstcrat40~C A&r 30 mioutes we pick up with a pipette, oae goutof the &em md we take it to the object port to observed with a Iiile msgnification, and we found seversI motive larvae, we dye with lug01 sod identificate. of 84 molluscs that we attalized 31 were similar tha” the third sta8e larvae of the -odes An8iostrongylus costuricmcis. This method its easy, and have a faster elaboration with a higher positivism