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Selected abstracts of the 16th annual meeting of the environmental mutagen society of Japan
Mutation Research, 203 (1988) 367-394
367
Elsevier MTR 08698
Environmental Mutagen Society of Japan Selected abstracts of the 16th Annual Meeting of the Environmental Mutagen Society of Japan
27-28 October 1987, Kyoto (Japan) (Received 22 April 1988) • (Accepted 28 April 1988)
Keywords: Japanese Environmental Mutagen Society; Abstracts; Annual Meeting 1987 J
Contents 1 Chen Deqing, N. Hachiya and Y. Takizawa, Department of Public Health, Akita University School of Medicine, Hondo 1 chome, Akita 010 (Japan), Induction of micronuclei by X-ray and tritiated water in the mouse 2 Endo G., K. Kuroda, I. Kiyota and S. Horiguchi, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545 (Japan), Genotoxicity of organoarsenic compounds 3 Fujie, K., S. Maeda 1 and T. Sugiyama 1, Laboratory of Environmental Science, Department of Natural Science, Osaka Women's University, Daisencho, Sakai, Osaka 590, and 1 Department of Pathology, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Kobe 650 (Japan), Mutagenicity of tyramine, (-)-(1S,3S)-l-methyl-l,2,3,4tetrahydro-fl-carboline-3-carboxylic acid (MTCA), NaC1 and soy sauce 4 Furihata, C., Y. Sato and T. Matsushima, Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108 (Japan), Alkaline elution of DNA from stomach pyloric mucosa of rats treated with MNNG and glyoxal 5 Hasegawa, M., Y. Nishi, H. Tsuda, N. Inui and K. Morimoto 1, Biological Research Center, Japan Tobacco Inc., Hatano, Kanagawa 257, and 1 Department of Public Health, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113 (Japan), Effects of diesel exhaust particles on chromosome aberration, sister-chromatid exchange and morphological transformation in cultured mammalian cells 6 Hashizume, T., K. Kosaka, H. Tsujisawa, T. Ozawa a, N. Kinae 1 and I. Tomita 1, Wakayama Prefectural Research Center of Environment and Public Health, Wakayama 640, and 1 Shizuoka College of Pharmaceutical Sciences, Shizuoka 422 (Japan), Detec-
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tion of direct mutagens and promutagens from broiled mutton Hayashi, M., T. Sofuni and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), A statistical procedure for rodent micronucleus tests The Collaborative Study Group for the Micronucleus Test (JEMS.MMS), Chief organizer, M. Hayashi, Division of Mutagenesis, National Institute of Hygienic Sciences, Tokyo 158 (Japan), Administration route difference in the micronucleus test Higo, H., K. Fujikawa 1 and Y. Satow, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima 734, and i Central Research Division, Takeda Chemical Ind., Ltd., Osaka 532 (Japan), DNA-damaging potency in vivo of 4- and 2-aminobiphenyls as measured in the Drosophila DNA repair test Hisaoka, S., K. Fujii, Y. Ohno and N. Muraoka, Sanyo Gakuen Women's Junior College, Okayama 703 (Japan), Mutagenicity in cooked foods. Effect of flavoring method and cooking oils on the formation of mutagenicity in sauteed meats Hochi, S., N. Yajima, T. Kishi and G. Kawanishi, Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Ishibashi, Shimotsuga-gun, Tochigi 329-05 (Japan), Comparative studies on mutagenicity of muscarinic agonists Iwawaki, H., M. Ito, S. Kogiso and A. Yoshitake, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Takatsukasa, Takarazuka, Hyogo 665 (Japan), Effects of L-ascorbic acid on the clastogenicity of catecholamines Kasai, H., T. Shintani and S. Nishimura, Biology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104 (Japan), Formation of 8-hydroxydeoxyguanosine in DNA by auto-oxidized unsatured fatty acids
0165-1161/88/$03.50 © 1988 Elsevier Science Publishers B.V. (Biomedical Division)
368 14 Kato, T., and K. Kikugawa, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 19203 (Japan), Formation of mutagenic diazoquinone by interaction of phenol with nitrite 15 Kawasaki, Y., H. Tamaki, T. Nunoshiba 1 and H. Nishioka l, Department of Home Economics, Doshisha Women's College, and 1 Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Antimutagenic activity of eggplant (Soranum melongena L.) extract 16 Kinae, N., M. Yamashita, N. Daicho, I. Tomita and I. Kimura 1, Shizuoka College of Pharmaceutical Sciences, Shizuoka 422, and 1 Aichi Cancer Center Research Institute, Nagoya 464 (Japan), Mutagenicity of pulp and paper mill effluents, and their tumor-inducing effect on catfish (Plotosus anguilla-
ris ) 17 Kong, Z.-L., M. Mitsuiki, M. Nonaka and H. Omura, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka 812 (Japan), Mutagenic activities of furfurals and the effects of Cu 2+ 18 Kurihara, K., S. Kurashina, Y. Tabuse 1, j. Miwa 1 and T. Kaminuma, Department of Bioinformatics, TIMS, Bunkyo-ku, Tokyo 113, and 1 NEC, Kawasaki City, Kanagawa 213 (Japan), Evaluation of the tumor promoter-screening system with the nematode C. elegans 19 Kuroda, Y., Laboratory of Phenogenetics, National Institute of Genetics, Mishima, Shizuoka 411 (Japan), Mutagen-modifying effects of vitamin A and vitamin E in mammalian cells in culture 20 Matsui, M., K. Matsui, T. Nohmi, H. Mizusawa and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Mutagenicity of steviol: An analytical approach using a Southern blotting system 21 Matsuoka, A., M. Sawada, T. Sofuni and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Chromosome aberration test on 25 chemicals - - Cooperative study with NTP of U.S.A. 22 Matsushita, H., A. Ono, K. Takeda and M. Mochizuki, Kyoritsu College of Pharmacy, Minatoku, Tokyo 105 (Japan), Mutagenicity and activation of alkylhydrazines 23 Meng, Z., Y. Sakail, y. Ose2, T. Sato2, H. Nagase 2, H. Kito 2, H. Matsuda 2, M. Sato 2 and M. Mizuno 2, China Pharmaceutical University, Nanjing (People's Republic of China), I Gifu Prefectural Institute of Public Health, Gifu 500, and 2 Gifu Pharmaceutical University, Gifu 502 (Japan), Antimutagenic activity of medical plants in traditional Chinese medicines 24 Miyamae, Y., O. Hiral, K. Ohara, M. Hashimoto and A. Tensho, Toxicology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Yodogawa, Osaka 532 (Japan), Clastogenic activity of FK973, a new antitumor agent
25 Miyata, M., K. Kabaya, H. Inoue, K. Yoshikawa, K. Nagata 1 and R. Kato 1, Toxicology Lab., Research Center, Mitsubishi Chemical Ind. Ltd., 1000 Kamoshida-cho, Midori-ku, Kanagawa 227, and i Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160 (Japan), Detection of Drosophila P-450s using anti-rat P-450 antibodies 26 Morita, T., Y. Watanabe, K. Takeda and K. Okumura, Tokyo Research Laboratories, Shin-Nihon Jitsugyou Co., Ltd., 3-25-3 Yahara, Nerima-ku, Tokyo 177 (Japan), Effect of pH in the in vitro chromosomal aberration test 27 Murota, T., M. Miyagawa, Y. Inoue, K. Nagaike and K. Yoshikawa, Mitsubishi Chemical Industries Ltd., Research Center, Kamoshida 1000, Midori-ku, Yokohama, Kanagawa 227 (Japan), Development of a mouse hemoglobin mutation assay 28 Nakamura, S., I. Oki and K. Sugimoto, Osaka Prefectural Institute of Public Health, Nakamichi-1, I-Iigashinari-ku, Osaka 537 (Japan), The suppressive effect of urine on the SOS response induced by chemical mutagens 29 Negishi, T., and H. Hayatsu, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700 (Japan), Use of Drosophila wing spot test for detection of mutagenesis by UVA irradiation and by cooperative action of UVA irradiation and 8-methoxypsoralen 30 Nishifuji, K., T. Kinouchi and Y. Ohnishi, Department of Bacteriology, School of Medicine, University of Tokushima, Tokushima 770 (Japan), Species difference in the formation of 1-nitropyrene oxides from 1-nitropyrene by hepatic microsome fractions 31 Nishifuji, K., T. Kinouchi and Y. Ohnishi, Department of Bacteriology, School of Medicine, University of Tokushima, Tokushima 770 (Japan), Stability of nitropyrenes in environmental water and soil 32 Nishikawa, T., H. Kaneta and S. Kusunoki, Life Science Laboratory, Advance Co., Ltd., 1-35, Shimo-ishilaara, Chofu, Tokyo 182 (Japan), A study on the cigarette-filter materials capable of adsorbing mutagens in cigarette smoke 33 Noguchi, T., A. Araki, F. Takahashi and T. Matsushima 1, Japan Industrial Safety and Health Association, Minato-ku, Tokyo 108, and 1 Institute in Medical Science, University of Tokyo, Tokyo 108 (Japan), New method in Ames Salmonella/ microsome assay for gaseous chemicals 34 Nomoto, K., M. Ineyama, M. Murakami, S. Kogiso, A. Yoshitake, J. Miyamoto and T. Kakunaga 1, Takarazuka Research Center, Sumitomo Chemical Co., Takatsukasa, Takarazuka, Hyogo 665, and 1 Research Institute for Microbial Disease, Osaka University, Nakanoshima, Kita-ku, Osaka 530 (Japan), A study on cell transformation assay with Balb/c 3T3 cells 35 Norppa, H., M. Hayashi, T. Sofuni, Y. Kodama and
369 M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Micronucleus test by flow cytometry: application to mouse peripheral blood 36 Nunoshiba, T., and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), A new assay system 'Rec-lac test' for detecting SOS-inducing genotoxic substances 37 Nunoshiba, T., M. Takahagi and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Mutagenicity and SOS-inducing activity of quinoxaline 1,4-dioxide derivatives in
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38 Oda, Y., S. Nakamura and I. Oki, Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka 537 (Japan), A simple test system (umu test) for the detection of environmental mutagens. VII. Inhibitory effects of curcumin on the SOS response induced by UV and chemical mutagens 39 Ohara, Y., T. Hayatsu, Y. Saito, M. Mifune, Y. Tanaka, N. Muraoka 1 and H. Hayatsu, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700, and 1 San-yo Gakuen Women's Junior College, Hirai, Okayama 703 (Japan), Extraction of heterocyclic amines from cooked food by using blue resin 40 Osada, K., and S. Kimura, Laboratory of Nutrition, Faculty of Agriculture, Tohoku University, Sendai 980 (Japan), Characteristics of chemiluminescence from mutagens in $9 mix 41 Oya, Y., and K. Yamamoto 1, Department of Pathology, Kanagawa Prefectural College of Nursing and Medical Technology, Yokohama 241, and 1 Department of Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113 (Japan), The clastogenic actions of a histidineperoxide adduct derived from L-histidine and hydrogen peroxide 42 Saito, K., S. Nakagawa, S. Kogiso, I. Nakatsuka, A. Yoshitake and J. Miyamoto, Takarazuka Research Center, Sumitomo Chemical Co., Takatsukasa, Takarazuka, Hyogo 665 (Japan), A study on analysis of DNA adducts by 32P-postlabeling method 43 Sasagawa, C., M. Muramatsu and T. Matsushima, Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108 (Japan), Formation of direct mutagens from amino-imidazoazaarenes by nitrite treatment 44 Sasaki, Y., K. Ohyama, R. Endo and S. Izumikawa, Tokyo Metropolitan Research Institute for Environmental Protection, Koto-ku, Tokyo 185 (Japan), Mutagen formation in nitration of aza- and oxa-arenes 45 Sato, M., T. Nunoshiba and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Mutation-inhibitory effect of metal compounds. II. Selenium compounds 46 Sato, S., and N. Inui, Biological Research Center, Japan Tobacco Inc., 23 Nakogi, Hatano, Kanagawa
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257 (Japan), Mouse strain difference in the induction of micronuclei Shibuya, T., T. Murota 1, T. Tokiwa, H. Matsumoto, N. Horiya and T. Hara, Hatano Research Institute, FDSC, Hadano, Kanagawa 257, and i Mitsubishi Chemical Industries, Midori-ku, Yokohama 227 (Japan), N-Propyl-N-nitrosourea-induced recessive mutations in mouse spermatogonia Shimoi, K., Y. Okada, Y. Nakamura, T. Noro, I. Tomita, Y. Sasaki 1, H. Imanishi i and Y. Shirasu 1, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422, and 1 Institute of Environmental Toxicology, Kodaira, Tokyo 187 (Japan), Effect of cosmetic UV absorbers on UV- or MMCinduced mutagenesis, sister-chromatid exchanges and chromosome aberrations Shioya, M., K. Wakabayashi, K. Yamashita, M. Nagao and T. Sugimura, National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), Formation of 8-hydroxydeoxyguanosine in DNA treated with fecapentaenes Sofuni, T., M. Sawada, M. Hatanaka and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Induction of chromosomal aberrations in active oxygen-generating systems: Studies on soft X-rays in Chinese hamster cells in culture Suzuki, S., H. Atai and Y. Hatakeyama, Preclinical Research Laboratories, Central Institute for Experimental Animals, Miyamae-ku, Kawasaki 213 (Japan), Effect of fasting on micronuclei frequency during intragastric drug administration in mice Suzuki, Y., K. Hayashi, N. Mashizu and H. Shimizu, Department of Public Health, Jikei University School of Medicine, Minato-ku, Tokyo 105 (Japan), Effects of indomethacin on the micronucleus test in mice Takahashi, K., K. Sakumi 1, y. Nakabeppu 1, M. Sekiguchi 1 and Y. Kawazoe, Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, and 1 Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka 812 (Japan), Positive regulation of adaptive response by direct methylation of Ada protein Takahashi, S., T. Kato and K. Kikugawa, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03 (Japan), Formation of heterocyclic amine-like mutagens in roasted coffee beans Takeda, K., K. Koizumi and M. Mochizuki, Kyoritsu College of Pharmacy, Minato-ku, Tokyo 105 (Japan), Suppressive factors for direct-acting mutagenicity of N-nitroso compounds Tamai, K., H. Tezuka 1, y. Kuroda 1 and T. Kada 1, Health Sciences Research Institute, Yokohama, Kanagawa 240, and i National Institute of Genetics, Mishima, Shizuoka 411 (Japan), Studies on the combined effects of environmental mutagens on mammalian cells in culture, IV Tsuda, H., C. Shimizu, M. Taketomi, M. Hasegawa, A. Hamada, K. Miyanaga and N. Inui, Biological
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Research Center, Japan Tobacco Inc., 23 Nakogi, Hatano, Kanagawa 257 (Japan), Acrylamide: the induction of transformation, chromosomal aberration and DNA damage Ukawa, S., C. Tobita and M. Mochizuki, Kyoritsu College of Pharmacy, Minato-ku, Tokyo 105 (Japan), Mutagenic and alkylating activities of (E)- and (Z)alkanediazohydroxides Wakabayashi, K., M. Shioya, S. Grivas, S. De Flora 1, M. Nagao and T. Sugimura,National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), and 1 University of Genoa, Via Pastore, 1, 1-16132 Genoa (Italy), Direct-acting mutagens formed from aminoimidazoquinolinesby photo-irradiation Watabe, T., H. Okuda, K. Ogura and A. Sugiyama, Department of Hygienic Chemistry, Tokyo College of Pharmacy, Hachioji-shi, Tokyo 192-03 (Japan), Direct-acting mutagenicity toward Salmonella of O-sulfonates of carcinogenic hydroxymethyl-substituted polycyclicarenes and N-hydroxy-2-acetylaminofluorene as reactive metabolites Watanabe, M., T. Nohmi and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Cloning of Salmonella typhimurium genes for nitroreductase and acetyltransferase: construction of Salmonella highly sensitive to mutagenic nitroarenes Yajima, N., S. Hochi, T. Kishi and G. Kawanishi, Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Ishibashi, Shimotsuga-gun, Tochigi 329-05 (Japan), DNA single-strand breaks induced by 3 cross-linkingagents Yamashita, K., A. Umemoto, S., Grivas, S. Kato, S. Sato and T. Sugimura, Biochemistry Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), Reaction of N-hydroxyderivatives of heterocyclicamines with DNA Zhang, X.M., K. Wakabayashi, Z.C. Liu 1, T. Sugimura and M. Nagao, National Cancer Center Research Institute, Chuooku, Tokyo 104 (Japan), and I Harbin Medical University, Harbin (People's Republic of China), Mutagenic and carcinogenic heterocyclic amines in Chinese cooked food
1 Chen Deqing, N. Hachiya and Y. Takizawa, Department of Public Health, Akita University School of Medicine, H o n d o 1 chome, Akita 010 (Japan)
Induction of micronuclei by X-ray and tritiated water in the mouse Micronucleus induction by X-irradiation or by treatment with tritiated water (HTO) was com-
pared in the maternal bone marrow and in the fetal liver of mouse to obtain basic data for an estimation of genetic risk of H T O in the environment. The frequency of polychromatic erythrocytes (PCE) with micronuclei reached a maximum at 24 h after the irradiation in the bone marrow or at 12 h in the fetal liver, when pregnant mice were irradiated with 1 Gy X-ray on the 13th day of gestation. Dose-effect relationships were almost linear up to 1 Gy in the two tissues, and damage of the cell cycle at 1.5 G y lowered the frequency of micronucleus induction below that expected by a linear mode. In the irradiated fetal liver, frequency of micronucleated PCE varied through developmental stages, reflecting a shift in the cell division kinetics. Female mice on the 8th day of gestation were injected intraperitoneally with 5-120 /zCi/g of HTO, and the micronucleus induction was analyzed in the bone marrow and in the fetal liver at the fifth day after the injection. Accumulated doses of fl-ray for 5 days in the mouse were estimated to be 0.034-0.81 Gy. The frequency of micronucleated PCE at 0.81 Gy in the bone marrow was 0.77%, which was compared with 0.13% in the control, and in the fetal liver it was 1.06% and 0.21%, respectively. The frequency was slightly higher in the fetal liver than in the bone marrow. The difference seemed to be attributable to different sensitivity of aberration induction a n d / o r to difference in hematopoietic kinetics in the two tissues.
2 Endo, G., K. Kuroda, I. Kiyota and S. Horiguchi, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545 (Japan)
Genotoxicity of organoarsenic compounds Genotoxicity of 10 organoarsenic compounds was studied using the rec assay, the Salmonella/ microsome mutagenicity test and SCE with C H L cells. All the compounds were negative in the mutagenicity test. Two compounds, tetraphenylarsonium chloride and phenylarsine oxide, were positive in the rec assay. 7 compounds, o- and p-aminophenylarsonic acid, tetraphenylarsonium chloride, asozin, cacodylic acid, o-arsanilic acid,
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Elsevier MTR 08698
Environmental Mutagen Society of Japan Selected abstracts of the 16th Annual Meeting of the Environmental Mutagen Society of Japan
27-28 October 1987, Kyoto (Japan) (Received 22 April 1988) • (Accepted 28 April 1988)
Keywords: Japanese Environmental Mutagen Society; Abstracts; Annual Meeting 1987 J
Contents 1 Chen Deqing, N. Hachiya and Y. Takizawa, Department of Public Health, Akita University School of Medicine, Hondo 1 chome, Akita 010 (Japan), Induction of micronuclei by X-ray and tritiated water in the mouse 2 Endo G., K. Kuroda, I. Kiyota and S. Horiguchi, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545 (Japan), Genotoxicity of organoarsenic compounds 3 Fujie, K., S. Maeda 1 and T. Sugiyama 1, Laboratory of Environmental Science, Department of Natural Science, Osaka Women's University, Daisencho, Sakai, Osaka 590, and 1 Department of Pathology, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, Kobe 650 (Japan), Mutagenicity of tyramine, (-)-(1S,3S)-l-methyl-l,2,3,4tetrahydro-fl-carboline-3-carboxylic acid (MTCA), NaC1 and soy sauce 4 Furihata, C., Y. Sato and T. Matsushima, Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108 (Japan), Alkaline elution of DNA from stomach pyloric mucosa of rats treated with MNNG and glyoxal 5 Hasegawa, M., Y. Nishi, H. Tsuda, N. Inui and K. Morimoto 1, Biological Research Center, Japan Tobacco Inc., Hatano, Kanagawa 257, and 1 Department of Public Health, Faculty of Medicine, University of Tokyo, Bunkyo-ku, Tokyo 113 (Japan), Effects of diesel exhaust particles on chromosome aberration, sister-chromatid exchange and morphological transformation in cultured mammalian cells 6 Hashizume, T., K. Kosaka, H. Tsujisawa, T. Ozawa a, N. Kinae 1 and I. Tomita 1, Wakayama Prefectural Research Center of Environment and Public Health, Wakayama 640, and 1 Shizuoka College of Pharmaceutical Sciences, Shizuoka 422 (Japan), Detec-
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tion of direct mutagens and promutagens from broiled mutton Hayashi, M., T. Sofuni and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), A statistical procedure for rodent micronucleus tests The Collaborative Study Group for the Micronucleus Test (JEMS.MMS), Chief organizer, M. Hayashi, Division of Mutagenesis, National Institute of Hygienic Sciences, Tokyo 158 (Japan), Administration route difference in the micronucleus test Higo, H., K. Fujikawa 1 and Y. Satow, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Hiroshima 734, and i Central Research Division, Takeda Chemical Ind., Ltd., Osaka 532 (Japan), DNA-damaging potency in vivo of 4- and 2-aminobiphenyls as measured in the Drosophila DNA repair test Hisaoka, S., K. Fujii, Y. Ohno and N. Muraoka, Sanyo Gakuen Women's Junior College, Okayama 703 (Japan), Mutagenicity in cooked foods. Effect of flavoring method and cooking oils on the formation of mutagenicity in sauteed meats Hochi, S., N. Yajima, T. Kishi and G. Kawanishi, Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Ishibashi, Shimotsuga-gun, Tochigi 329-05 (Japan), Comparative studies on mutagenicity of muscarinic agonists Iwawaki, H., M. Ito, S. Kogiso and A. Yoshitake, Takarazuka Research Center, Sumitomo Chemical Co., Ltd., Takatsukasa, Takarazuka, Hyogo 665 (Japan), Effects of L-ascorbic acid on the clastogenicity of catecholamines Kasai, H., T. Shintani and S. Nishimura, Biology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104 (Japan), Formation of 8-hydroxydeoxyguanosine in DNA by auto-oxidized unsatured fatty acids
0165-1161/88/$03.50 © 1988 Elsevier Science Publishers B.V. (Biomedical Division)
368 14 Kato, T., and K. Kikugawa, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 19203 (Japan), Formation of mutagenic diazoquinone by interaction of phenol with nitrite 15 Kawasaki, Y., H. Tamaki, T. Nunoshiba 1 and H. Nishioka l, Department of Home Economics, Doshisha Women's College, and 1 Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Antimutagenic activity of eggplant (Soranum melongena L.) extract 16 Kinae, N., M. Yamashita, N. Daicho, I. Tomita and I. Kimura 1, Shizuoka College of Pharmaceutical Sciences, Shizuoka 422, and 1 Aichi Cancer Center Research Institute, Nagoya 464 (Japan), Mutagenicity of pulp and paper mill effluents, and their tumor-inducing effect on catfish (Plotosus anguilla-
ris ) 17 Kong, Z.-L., M. Mitsuiki, M. Nonaka and H. Omura, Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka 812 (Japan), Mutagenic activities of furfurals and the effects of Cu 2+ 18 Kurihara, K., S. Kurashina, Y. Tabuse 1, j. Miwa 1 and T. Kaminuma, Department of Bioinformatics, TIMS, Bunkyo-ku, Tokyo 113, and 1 NEC, Kawasaki City, Kanagawa 213 (Japan), Evaluation of the tumor promoter-screening system with the nematode C. elegans 19 Kuroda, Y., Laboratory of Phenogenetics, National Institute of Genetics, Mishima, Shizuoka 411 (Japan), Mutagen-modifying effects of vitamin A and vitamin E in mammalian cells in culture 20 Matsui, M., K. Matsui, T. Nohmi, H. Mizusawa and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Mutagenicity of steviol: An analytical approach using a Southern blotting system 21 Matsuoka, A., M. Sawada, T. Sofuni and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Chromosome aberration test on 25 chemicals - - Cooperative study with NTP of U.S.A. 22 Matsushita, H., A. Ono, K. Takeda and M. Mochizuki, Kyoritsu College of Pharmacy, Minatoku, Tokyo 105 (Japan), Mutagenicity and activation of alkylhydrazines 23 Meng, Z., Y. Sakail, y. Ose2, T. Sato2, H. Nagase 2, H. Kito 2, H. Matsuda 2, M. Sato 2 and M. Mizuno 2, China Pharmaceutical University, Nanjing (People's Republic of China), I Gifu Prefectural Institute of Public Health, Gifu 500, and 2 Gifu Pharmaceutical University, Gifu 502 (Japan), Antimutagenic activity of medical plants in traditional Chinese medicines 24 Miyamae, Y., O. Hiral, K. Ohara, M. Hashimoto and A. Tensho, Toxicology Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Yodogawa, Osaka 532 (Japan), Clastogenic activity of FK973, a new antitumor agent
25 Miyata, M., K. Kabaya, H. Inoue, K. Yoshikawa, K. Nagata 1 and R. Kato 1, Toxicology Lab., Research Center, Mitsubishi Chemical Ind. Ltd., 1000 Kamoshida-cho, Midori-ku, Kanagawa 227, and i Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160 (Japan), Detection of Drosophila P-450s using anti-rat P-450 antibodies 26 Morita, T., Y. Watanabe, K. Takeda and K. Okumura, Tokyo Research Laboratories, Shin-Nihon Jitsugyou Co., Ltd., 3-25-3 Yahara, Nerima-ku, Tokyo 177 (Japan), Effect of pH in the in vitro chromosomal aberration test 27 Murota, T., M. Miyagawa, Y. Inoue, K. Nagaike and K. Yoshikawa, Mitsubishi Chemical Industries Ltd., Research Center, Kamoshida 1000, Midori-ku, Yokohama, Kanagawa 227 (Japan), Development of a mouse hemoglobin mutation assay 28 Nakamura, S., I. Oki and K. Sugimoto, Osaka Prefectural Institute of Public Health, Nakamichi-1, I-Iigashinari-ku, Osaka 537 (Japan), The suppressive effect of urine on the SOS response induced by chemical mutagens 29 Negishi, T., and H. Hayatsu, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700 (Japan), Use of Drosophila wing spot test for detection of mutagenesis by UVA irradiation and by cooperative action of UVA irradiation and 8-methoxypsoralen 30 Nishifuji, K., T. Kinouchi and Y. Ohnishi, Department of Bacteriology, School of Medicine, University of Tokushima, Tokushima 770 (Japan), Species difference in the formation of 1-nitropyrene oxides from 1-nitropyrene by hepatic microsome fractions 31 Nishifuji, K., T. Kinouchi and Y. Ohnishi, Department of Bacteriology, School of Medicine, University of Tokushima, Tokushima 770 (Japan), Stability of nitropyrenes in environmental water and soil 32 Nishikawa, T., H. Kaneta and S. Kusunoki, Life Science Laboratory, Advance Co., Ltd., 1-35, Shimo-ishilaara, Chofu, Tokyo 182 (Japan), A study on the cigarette-filter materials capable of adsorbing mutagens in cigarette smoke 33 Noguchi, T., A. Araki, F. Takahashi and T. Matsushima 1, Japan Industrial Safety and Health Association, Minato-ku, Tokyo 108, and 1 Institute in Medical Science, University of Tokyo, Tokyo 108 (Japan), New method in Ames Salmonella/ microsome assay for gaseous chemicals 34 Nomoto, K., M. Ineyama, M. Murakami, S. Kogiso, A. Yoshitake, J. Miyamoto and T. Kakunaga 1, Takarazuka Research Center, Sumitomo Chemical Co., Takatsukasa, Takarazuka, Hyogo 665, and 1 Research Institute for Microbial Disease, Osaka University, Nakanoshima, Kita-ku, Osaka 530 (Japan), A study on cell transformation assay with Balb/c 3T3 cells 35 Norppa, H., M. Hayashi, T. Sofuni, Y. Kodama and
369 M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Micronucleus test by flow cytometry: application to mouse peripheral blood 36 Nunoshiba, T., and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), A new assay system 'Rec-lac test' for detecting SOS-inducing genotoxic substances 37 Nunoshiba, T., M. Takahagi and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Mutagenicity and SOS-inducing activity of quinoxaline 1,4-dioxide derivatives in
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38 Oda, Y., S. Nakamura and I. Oki, Osaka Prefectural Institute of Public Health, Higashinari-ku, Osaka 537 (Japan), A simple test system (umu test) for the detection of environmental mutagens. VII. Inhibitory effects of curcumin on the SOS response induced by UV and chemical mutagens 39 Ohara, Y., T. Hayatsu, Y. Saito, M. Mifune, Y. Tanaka, N. Muraoka 1 and H. Hayatsu, Faculty of Pharmaceutical Sciences, Okayama University, Tsushima, Okayama 700, and 1 San-yo Gakuen Women's Junior College, Hirai, Okayama 703 (Japan), Extraction of heterocyclic amines from cooked food by using blue resin 40 Osada, K., and S. Kimura, Laboratory of Nutrition, Faculty of Agriculture, Tohoku University, Sendai 980 (Japan), Characteristics of chemiluminescence from mutagens in $9 mix 41 Oya, Y., and K. Yamamoto 1, Department of Pathology, Kanagawa Prefectural College of Nursing and Medical Technology, Yokohama 241, and 1 Department of Cytogenetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113 (Japan), The clastogenic actions of a histidineperoxide adduct derived from L-histidine and hydrogen peroxide 42 Saito, K., S. Nakagawa, S. Kogiso, I. Nakatsuka, A. Yoshitake and J. Miyamoto, Takarazuka Research Center, Sumitomo Chemical Co., Takatsukasa, Takarazuka, Hyogo 665 (Japan), A study on analysis of DNA adducts by 32P-postlabeling method 43 Sasagawa, C., M. Muramatsu and T. Matsushima, Department of Molecular Oncology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108 (Japan), Formation of direct mutagens from amino-imidazoazaarenes by nitrite treatment 44 Sasaki, Y., K. Ohyama, R. Endo and S. Izumikawa, Tokyo Metropolitan Research Institute for Environmental Protection, Koto-ku, Tokyo 185 (Japan), Mutagen formation in nitration of aza- and oxa-arenes 45 Sato, M., T. Nunoshiba and H. Nishioka, Division of Biochemistry, Doshisha University, Kyoto 602 (Japan), Mutation-inhibitory effect of metal compounds. II. Selenium compounds 46 Sato, S., and N. Inui, Biological Research Center, Japan Tobacco Inc., 23 Nakogi, Hatano, Kanagawa
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257 (Japan), Mouse strain difference in the induction of micronuclei Shibuya, T., T. Murota 1, T. Tokiwa, H. Matsumoto, N. Horiya and T. Hara, Hatano Research Institute, FDSC, Hadano, Kanagawa 257, and i Mitsubishi Chemical Industries, Midori-ku, Yokohama 227 (Japan), N-Propyl-N-nitrosourea-induced recessive mutations in mouse spermatogonia Shimoi, K., Y. Okada, Y. Nakamura, T. Noro, I. Tomita, Y. Sasaki 1, H. Imanishi i and Y. Shirasu 1, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422, and 1 Institute of Environmental Toxicology, Kodaira, Tokyo 187 (Japan), Effect of cosmetic UV absorbers on UV- or MMCinduced mutagenesis, sister-chromatid exchanges and chromosome aberrations Shioya, M., K. Wakabayashi, K. Yamashita, M. Nagao and T. Sugimura, National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), Formation of 8-hydroxydeoxyguanosine in DNA treated with fecapentaenes Sofuni, T., M. Sawada, M. Hatanaka and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Induction of chromosomal aberrations in active oxygen-generating systems: Studies on soft X-rays in Chinese hamster cells in culture Suzuki, S., H. Atai and Y. Hatakeyama, Preclinical Research Laboratories, Central Institute for Experimental Animals, Miyamae-ku, Kawasaki 213 (Japan), Effect of fasting on micronuclei frequency during intragastric drug administration in mice Suzuki, Y., K. Hayashi, N. Mashizu and H. Shimizu, Department of Public Health, Jikei University School of Medicine, Minato-ku, Tokyo 105 (Japan), Effects of indomethacin on the micronucleus test in mice Takahashi, K., K. Sakumi 1, y. Nakabeppu 1, M. Sekiguchi 1 and Y. Kawazoe, Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, and 1 Department of Biochemistry, Faculty of Medicine, Kyushu University, Fukuoka 812 (Japan), Positive regulation of adaptive response by direct methylation of Ada protein Takahashi, S., T. Kato and K. Kikugawa, Tokyo College of Pharmacy, 1432-1 Horinouchi, Hachioji, Tokyo 192-03 (Japan), Formation of heterocyclic amine-like mutagens in roasted coffee beans Takeda, K., K. Koizumi and M. Mochizuki, Kyoritsu College of Pharmacy, Minato-ku, Tokyo 105 (Japan), Suppressive factors for direct-acting mutagenicity of N-nitroso compounds Tamai, K., H. Tezuka 1, y. Kuroda 1 and T. Kada 1, Health Sciences Research Institute, Yokohama, Kanagawa 240, and i National Institute of Genetics, Mishima, Shizuoka 411 (Japan), Studies on the combined effects of environmental mutagens on mammalian cells in culture, IV Tsuda, H., C. Shimizu, M. Taketomi, M. Hasegawa, A. Hamada, K. Miyanaga and N. Inui, Biological
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Research Center, Japan Tobacco Inc., 23 Nakogi, Hatano, Kanagawa 257 (Japan), Acrylamide: the induction of transformation, chromosomal aberration and DNA damage Ukawa, S., C. Tobita and M. Mochizuki, Kyoritsu College of Pharmacy, Minato-ku, Tokyo 105 (Japan), Mutagenic and alkylating activities of (E)- and (Z)alkanediazohydroxides Wakabayashi, K., M. Shioya, S. Grivas, S. De Flora 1, M. Nagao and T. Sugimura,National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), and 1 University of Genoa, Via Pastore, 1, 1-16132 Genoa (Italy), Direct-acting mutagens formed from aminoimidazoquinolinesby photo-irradiation Watabe, T., H. Okuda, K. Ogura and A. Sugiyama, Department of Hygienic Chemistry, Tokyo College of Pharmacy, Hachioji-shi, Tokyo 192-03 (Japan), Direct-acting mutagenicity toward Salmonella of O-sulfonates of carcinogenic hydroxymethyl-substituted polycyclicarenes and N-hydroxy-2-acetylaminofluorene as reactive metabolites Watanabe, M., T. Nohmi and M. Ishidate Jr., Division of Mutagenesis, National Institute of Hygienic Sciences, Setagaya-ku, Tokyo 158 (Japan), Cloning of Salmonella typhimurium genes for nitroreductase and acetyltransferase: construction of Salmonella highly sensitive to mutagenic nitroarenes Yajima, N., S. Hochi, T. Kishi and G. Kawanishi, Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Ishibashi, Shimotsuga-gun, Tochigi 329-05 (Japan), DNA single-strand breaks induced by 3 cross-linkingagents Yamashita, K., A. Umemoto, S., Grivas, S. Kato, S. Sato and T. Sugimura, Biochemistry Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104 (Japan), Reaction of N-hydroxyderivatives of heterocyclicamines with DNA Zhang, X.M., K. Wakabayashi, Z.C. Liu 1, T. Sugimura and M. Nagao, National Cancer Center Research Institute, Chuooku, Tokyo 104 (Japan), and I Harbin Medical University, Harbin (People's Republic of China), Mutagenic and carcinogenic heterocyclic amines in Chinese cooked food
1 Chen Deqing, N. Hachiya and Y. Takizawa, Department of Public Health, Akita University School of Medicine, H o n d o 1 chome, Akita 010 (Japan)
Induction of micronuclei by X-ray and tritiated water in the mouse Micronucleus induction by X-irradiation or by treatment with tritiated water (HTO) was com-
pared in the maternal bone marrow and in the fetal liver of mouse to obtain basic data for an estimation of genetic risk of H T O in the environment. The frequency of polychromatic erythrocytes (PCE) with micronuclei reached a maximum at 24 h after the irradiation in the bone marrow or at 12 h in the fetal liver, when pregnant mice were irradiated with 1 Gy X-ray on the 13th day of gestation. Dose-effect relationships were almost linear up to 1 Gy in the two tissues, and damage of the cell cycle at 1.5 G y lowered the frequency of micronucleus induction below that expected by a linear mode. In the irradiated fetal liver, frequency of micronucleated PCE varied through developmental stages, reflecting a shift in the cell division kinetics. Female mice on the 8th day of gestation were injected intraperitoneally with 5-120 /zCi/g of HTO, and the micronucleus induction was analyzed in the bone marrow and in the fetal liver at the fifth day after the injection. Accumulated doses of fl-ray for 5 days in the mouse were estimated to be 0.034-0.81 Gy. The frequency of micronucleated PCE at 0.81 Gy in the bone marrow was 0.77%, which was compared with 0.13% in the control, and in the fetal liver it was 1.06% and 0.21%, respectively. The frequency was slightly higher in the fetal liver than in the bone marrow. The difference seemed to be attributable to different sensitivity of aberration induction a n d / o r to difference in hematopoietic kinetics in the two tissues.
2 Endo, G., K. Kuroda, I. Kiyota and S. Horiguchi, Osaka City University Medical School, Asahimachi, Abeno-ku, Osaka 545 (Japan)
Genotoxicity of organoarsenic compounds Genotoxicity of 10 organoarsenic compounds was studied using the rec assay, the Salmonella/ microsome mutagenicity test and SCE with C H L cells. All the compounds were negative in the mutagenicity test. Two compounds, tetraphenylarsonium chloride and phenylarsine oxide, were positive in the rec assay. 7 compounds, o- and p-aminophenylarsonic acid, tetraphenylarsonium chloride, asozin, cacodylic acid, o-arsanilic acid,