Sequencing of soy oil and soy lecithin allergens

S152 Abstracts

SUNDAY

507

Sequencing of Soy Oil and Soy Lecithin Allergens

Y. Errahali1, G. Kanny1, M. Morisset1, C. Cren-Olive2, C. Rolando2, M. Metche1, D. A. Moneret-Vautrin1, J. P. Nicolas1, S. Fremont1; 1Faculty of Medicine, University Nancy I, Vandoeuvre les Nancy, FRANCE, 2Laboratoire de Chimie organique et Macromoleculaire, Université des Science et Technologies de Lille, Lille, FRANCE. RATIONALE: It has been shown that soy oil and soy lecithin may contain allergens. The aim of the present study is to characterize, by means of the physico-chemical and immunological methods, the allergenic proteins of soybean seed transferred to soy oil and soy lecithin during industrial refining. METHODS: Extraction of proteins was conducted using a buffer solution. The identification of allergens used specific IgE from a soybean allergic patient. Bi-dimensional electrophoresis and sequencing from N and C terminals were used to characterize the allergenic proteins. RESULTS: Two allergenic proteins present in soybean seed, soy oil and soy lecithin have been isolated and identified: one 56 kDa non glycosylated allergen, whose isoelectric point of 5.4 has been identified as soybean β-Amylase (7S). One 20 kDa non glycosylated allergen, whose isoelectric point of 4.6 has been identified as Kunitz Trypsin inhibitor [Glycine max] (2S). CONCLUSIONS: We have sequenced two allergenic proteins present as hidden allergens in soy oil and soy lecithin. It is to note that these highly refining-resistant proteins are not the main proteins of soybean seed. Funding: Faculty of Medicine

J ALLERGY CLIN IMMUNOL FEBRUARY 2004