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Serum adenosine deaminase activity in scleroderma patients
387
BLOODCOAGUUTION EXANINATIONIN PROGRESIVESYSTEHIC SCLEROSIS (PSS)---PLASMA LEVELS OF THROMBIN . ANTITHROMBINLB COMPLEX(TAT) AND ANTITHROMBIN m (ATDI)--MANABU MEDA, YUKIKOSHIKANO,AND SHUNJI NOR1 Department of Dermatology, Gifu University School
of Medicine,
We examined blood coagulation in 45 cases of PSS.especially fibrinogen@bg). prothrombin time(PT). active partial thromboplastin time(APTT), TAT, ATIII. plasninqen@ng). PDP, D-diner, crZ plasmin Inhibitor-plasmin complex(PICj. soluble fibrin monomer complex (SPMC) in order to clarify the disorder of platelet-blood vessel system. As the result. plasma levels of TAT and ATUl were high in 29 of 39 cases(74.4%), 23 of 45 cases(51.1%), respectively. There was no statistical negative correlation between those of TAT and ATlll. Plasma levels of PIC. D-dimer. FDP and SFNC were not always high except for several cases. and lnoreover there was no statistical positive correlation between those of TAT and PIC. These data lead us to recomfirm some disorder vessel system in PSS.
of platelet-
blood
SERUM ADENOSINE DEAMINASE ACTIVITY IN SCLERODERNA PATIENTS
TETSLJOSASAKI AND HIROSHI NAKAJIMA. Department of Dermatology, Yokohama City University of Medicine, Yokohama, Japan
School
PRODUCTION AND CHARACTERIZATION TO TREPONEMA PALLIDUM
OF MONOCLONAL
ANTIBODIES
Min Geol Lee, Kyu Kwang Whang, Wook Lew. Jung Bock Lee Department of Dermatology. Yonsei University College of Medicine, Seoul, Korea Murine monoclonal antibodies against Treponema pallidurn were iwlated by measuring optical denshies or antibody tfters by enzyme-linked immunosorbent assay, indirect immunofluorescence or hemagglutination techmques. The isotypes of the 7 monoclinal antibodies produced were IgGl. lgG2a and IgGPb. Six of the seven monoclonal antibodtes were directed specifically against T. paltidum antigen. Five of the six monoclinal antibodies were raised against a polypeptide of a molecular weight of 47 kDa. One monoclonal antibody was bound to T. pallidurn, T phagedenis, T. denticola and T. vincentb, thus demonstrating a possible specificity for a treponema group antigen. This was raised against a polypeptide of a molecular weight of 64 kDa ELISA-inhibition tests wlth these monoclonal antibodies showed that there was a statistically significant difference in the degree of inhibition between groups of healthy controls and of each stage of syphilis. Therefore, the specific anttbodies reacting with major antigenic polypeptides of a molecular weight of 47 kDa could be adapted in the diagwxis 01 syphilis
;
CO-EXPRESSIONOF FILACCRIN AND TRICHOHYALININ NORMALAND ABNORMAL KERATINIZED EPITHELIUM MOTOMU NANABE”‘, W.MICHAELO’GUIN”. BEVEILY A. DALE,‘, TUNGTIEN SUN’, JUNTENDOUNIVERSITY, TOKYO’, NEWYORK UNIV., NEWYORK”, AND UNIV. OF WASHINGTON,SEATTLE‘.
Adenosine deaminase (ADA) is one of the enzymes of the purine metabolic pathway. In serum, ADA activity is mainly derived from T lymphocytes. The purpose of this study is to clarify the significance of ADA in scleroderma. Serum ADA activity was determined with enzymatic method in 28 patients with systemic scleroderma (PSS), 4 localized scleroderma, 4 MCTD, 6 dermaromyosiris (DM) and 14 with other related diseases, 53 (95 X) of which had antinuclear antibodies. Serum ADA activity was elevated in 89 4,of PSS, all of localized scleroderma and MCTD, 83 % of DM and 71 % of the other related diseases. The mean values in 9 PSS with anti Scl-70 antibodies, 6 PSS with anti RNP antibodies and 4 MCTD, and 8 PSS and I generalized morphea with anticentromere antibodies were 26.7, 25.6 and 22.2 IlJ/l,respectively.These results suggest that serum ADA activity may well reflect T cell activation in scleroderma and related disorders.
Keratohyalin and trlchohyalin granules are classically thouaht to retresent differentiation markers uniaue tu the epidermis and’ inner root sheath/ medulla of hair ‘foil icles, respectively. Using antinbodies specific for the protein subunits of these two types of granules. we discovered important exceptions to this rule. Keratohyalln and trichohyalin proteins co-exist in 1) papilla of the dorsal tongue epithelium and 2) bullous congenital ichthyosiform erythroderma and psoriasis. Furthermore, on the basis of ultrastructural double labellinn. we are now able to show that keratohyalin and trichohyaiin proteins are topological segregated in the same granuies forming hybrid Thus provided evidence that keratohyalin and granules. trichohyalin proteins undergo highly coordinated pathways of synthesis,, during the terminal differentiation of normal tongue eplthelium and some abnormal keratinlzed epithelium.
SERUM SCSB-9 LEVELS AS A SENSITIVE INDICATOR OF HENOCW-SCHONLEINPURPURA ACTIVITY
A NEW MONOCLONAL ANTIBODY AGAINST HEMIDESMOSOMES OF HUMAN EPIDERMAL BASAL CELLS
SEIJI KAWANA, YUSUKE SUZUKI'/,AND TOSHIYA ASAIII. Department of Dermatology, ST. Luke's InternationalHospital, Tokyo, Kitasato Univ. School of Medicine, Sagamiharail
XIAO-MIN ZHANG, YUJI HORIGUCHI, SADAO IMAMURA. Department of Dermatology, Faculty of Medicine, Kyoto University, Kyoto Using monoclonal antibody technology, a new antibasement
The concentration of the terminal complement complex (TCC), SC5b-9, was determined for 48 serum samples from 30 patients with Henoch-Schonleinpurpura. In 25 of the patients, TCC concentrationwas found to increase significantly in the active phase of the disease. Three patients with various manifestations of the disease were followed longitudinally for a period of several years covering in active and inactive phases. TCC elevation in all cases was noted to be correlated with exacerbations of the disease. C3 and C4 or CH50 levels in many instances remained normal or increased and thus were not reliable indicators of disease activity. Measurement of TCC should thus prove quite useful for monitoring the activity of Henoch-Schonleinpurpura in patients in whom complement activation is pathognomonic.
membrane zone (BMZ) antibody, designated as 2B7B has been derived from human prostate gland. Immunohistochemical ass& ravealed rhat this antigen is expressed not only along BMZ of the acini of prosfate gland but also along BMZ of the epidermis, appendages and epithelium of the esophagus, urinary bladder and ureter, as well as endorhelial cells of blood vessel. From the dermatologicalpoint, this antibody is interesting as it reacts similarly to the bullous pemphigoid antibodies to the hemidesmosomesof the epidermal base1 cell, though it does not compete the BP antibodies. This antibody seems much available to dermatoiogical investigation,such as the diagnosis of squamous cell carcinoma, basal cell epirhelioma and skin appendage tumors.
BLOODCOAGUUTION EXANINATIONIN PROGRESIVESYSTEHIC SCLEROSIS (PSS)---PLASMA LEVELS OF THROMBIN . ANTITHROMBINLB COMPLEX(TAT) AND ANTITHROMBIN m (ATDI)--MANABU MEDA, YUKIKOSHIKANO,AND SHUNJI NOR1 Department of Dermatology, Gifu University School
of Medicine,
We examined blood coagulation in 45 cases of PSS.especially fibrinogen@bg). prothrombin time(PT). active partial thromboplastin time(APTT), TAT, ATIII. plasninqen@ng). PDP, D-diner, crZ plasmin Inhibitor-plasmin complex(PICj. soluble fibrin monomer complex (SPMC) in order to clarify the disorder of platelet-blood vessel system. As the result. plasma levels of TAT and ATUl were high in 29 of 39 cases(74.4%), 23 of 45 cases(51.1%), respectively. There was no statistical negative correlation between those of TAT and ATlll. Plasma levels of PIC. D-dimer. FDP and SFNC were not always high except for several cases. and lnoreover there was no statistical positive correlation between those of TAT and PIC. These data lead us to recomfirm some disorder vessel system in PSS.
of platelet-
blood
SERUM ADENOSINE DEAMINASE ACTIVITY IN SCLERODERNA PATIENTS
TETSLJOSASAKI AND HIROSHI NAKAJIMA. Department of Dermatology, Yokohama City University of Medicine, Yokohama, Japan
School
PRODUCTION AND CHARACTERIZATION TO TREPONEMA PALLIDUM
OF MONOCLONAL
ANTIBODIES
Min Geol Lee, Kyu Kwang Whang, Wook Lew. Jung Bock Lee Department of Dermatology. Yonsei University College of Medicine, Seoul, Korea Murine monoclonal antibodies against Treponema pallidurn were iwlated by measuring optical denshies or antibody tfters by enzyme-linked immunosorbent assay, indirect immunofluorescence or hemagglutination techmques. The isotypes of the 7 monoclinal antibodies produced were IgGl. lgG2a and IgGPb. Six of the seven monoclonal antibodtes were directed specifically against T. paltidum antigen. Five of the six monoclinal antibodies were raised against a polypeptide of a molecular weight of 47 kDa. One monoclonal antibody was bound to T. pallidurn, T phagedenis, T. denticola and T. vincentb, thus demonstrating a possible specificity for a treponema group antigen. This was raised against a polypeptide of a molecular weight of 64 kDa ELISA-inhibition tests wlth these monoclonal antibodies showed that there was a statistically significant difference in the degree of inhibition between groups of healthy controls and of each stage of syphilis. Therefore, the specific anttbodies reacting with major antigenic polypeptides of a molecular weight of 47 kDa could be adapted in the diagwxis 01 syphilis
;
CO-EXPRESSIONOF FILACCRIN AND TRICHOHYALININ NORMALAND ABNORMAL KERATINIZED EPITHELIUM MOTOMU NANABE”‘, W.MICHAELO’GUIN”. BEVEILY A. DALE,‘, TUNGTIEN SUN’, JUNTENDOUNIVERSITY, TOKYO’, NEWYORK UNIV., NEWYORK”, AND UNIV. OF WASHINGTON,SEATTLE‘.
Adenosine deaminase (ADA) is one of the enzymes of the purine metabolic pathway. In serum, ADA activity is mainly derived from T lymphocytes. The purpose of this study is to clarify the significance of ADA in scleroderma. Serum ADA activity was determined with enzymatic method in 28 patients with systemic scleroderma (PSS), 4 localized scleroderma, 4 MCTD, 6 dermaromyosiris (DM) and 14 with other related diseases, 53 (95 X) of which had antinuclear antibodies. Serum ADA activity was elevated in 89 4,of PSS, all of localized scleroderma and MCTD, 83 % of DM and 71 % of the other related diseases. The mean values in 9 PSS with anti Scl-70 antibodies, 6 PSS with anti RNP antibodies and 4 MCTD, and 8 PSS and I generalized morphea with anticentromere antibodies were 26.7, 25.6 and 22.2 IlJ/l,respectively.These results suggest that serum ADA activity may well reflect T cell activation in scleroderma and related disorders.
Keratohyalin and trlchohyalin granules are classically thouaht to retresent differentiation markers uniaue tu the epidermis and’ inner root sheath/ medulla of hair ‘foil icles, respectively. Using antinbodies specific for the protein subunits of these two types of granules. we discovered important exceptions to this rule. Keratohyalln and trichohyalin proteins co-exist in 1) papilla of the dorsal tongue epithelium and 2) bullous congenital ichthyosiform erythroderma and psoriasis. Furthermore, on the basis of ultrastructural double labellinn. we are now able to show that keratohyalin and trichohyaiin proteins are topological segregated in the same granuies forming hybrid Thus provided evidence that keratohyalin and granules. trichohyalin proteins undergo highly coordinated pathways of synthesis,, during the terminal differentiation of normal tongue eplthelium and some abnormal keratinlzed epithelium.
SERUM SCSB-9 LEVELS AS A SENSITIVE INDICATOR OF HENOCW-SCHONLEINPURPURA ACTIVITY
A NEW MONOCLONAL ANTIBODY AGAINST HEMIDESMOSOMES OF HUMAN EPIDERMAL BASAL CELLS
SEIJI KAWANA, YUSUKE SUZUKI'/,AND TOSHIYA ASAIII. Department of Dermatology, ST. Luke's InternationalHospital, Tokyo, Kitasato Univ. School of Medicine, Sagamiharail
XIAO-MIN ZHANG, YUJI HORIGUCHI, SADAO IMAMURA. Department of Dermatology, Faculty of Medicine, Kyoto University, Kyoto Using monoclonal antibody technology, a new antibasement
The concentration of the terminal complement complex (TCC), SC5b-9, was determined for 48 serum samples from 30 patients with Henoch-Schonleinpurpura. In 25 of the patients, TCC concentrationwas found to increase significantly in the active phase of the disease. Three patients with various manifestations of the disease were followed longitudinally for a period of several years covering in active and inactive phases. TCC elevation in all cases was noted to be correlated with exacerbations of the disease. C3 and C4 or CH50 levels in many instances remained normal or increased and thus were not reliable indicators of disease activity. Measurement of TCC should thus prove quite useful for monitoring the activity of Henoch-Schonleinpurpura in patients in whom complement activation is pathognomonic.
membrane zone (BMZ) antibody, designated as 2B7B has been derived from human prostate gland. Immunohistochemical ass& ravealed rhat this antigen is expressed not only along BMZ of the acini of prosfate gland but also along BMZ of the epidermis, appendages and epithelium of the esophagus, urinary bladder and ureter, as well as endorhelial cells of blood vessel. From the dermatologicalpoint, this antibody is interesting as it reacts similarly to the bullous pemphigoid antibodies to the hemidesmosomesof the epidermal base1 cell, though it does not compete the BP antibodies. This antibody seems much available to dermatoiogical investigation,such as the diagnosis of squamous cell carcinoma, basal cell epirhelioma and skin appendage tumors.