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Serum apolipoprotein B48 concentration is high in patients with impaired glucose tolerance and increases after oral glucose ingestion in patients with diabetes mellitus
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Abstracts / Atherosclerosis 252 (2016) e1ee196
MELLITUS COMPLICATIONS CORRELATION BETWEEN HOMOCYSTEINE AND TYPE-2 DIABETES MELLITUS COMPLICATIONS M. Emukhvari 1, N. Zabakhidze 1, T. M. Akhvlediani 1, Kvantaliani 2. 1 Reasearch Institute of Clinical Medicine, Laboratory of Clinical Diagnostics, Tbilisi, Georgia; 2 Medical Centre “Healing Home”, Department of Cardiology, Tbilisi, Georgia Objectives: The damage of small blood vessels(microangiopathy), medial and large arteries(macroangiopathy) in diabetic angiopathy is connected with the disorders of carbohydrate metabolism and homocysteine levels. The aim of the study was association of homocysteine , HBA1c and lipid profile levels in patients with type-2diabetes. Methods: 50 diabetic patients (age 56.2 ± 1.3)( group I) and30 healthy adults(age 52.3±1.0)( II group) were recruited. In Group I were 33 men and 17 women. Group II consisted from 16 men and 14 women. The patients with type-2 diabetes were divided into two subgroups: subgroup I e ( N¼28) patients with associated complications , and subgroup II e 22 patients without complications of diabetes. Homocysteine as well HBA1c, lipids( total cholesterol, HDL, LDL and triglicerides) and urine microalbumine parameters were measured. Results: We found that homocysteine levels are significantly higher in complicated diabetes ( 22.0±0,7 mmol/l ) than in patients without complications of diabeted(17.1±0.8 mmol/l). Significantly elevated homocysteine levels were found in patients with CAD, stroke and neuropathy as compared to control group. There was a positive correlation between homocysteine, total cholesterol, LDL andTG levels(r¼ 3.44, r¼3.67, and r¼ 4.0). The correlation between microalbuminuria and homocystein were also positive(r¼ 3.92). Conclusions: In patients who have developed micro/macro angiopathy of vessels we founded hyperhomocysteinemia with lipid profile disorders and high level of HBA1c. ..Taking into consideration the result obtained, we think it is possible to use positive correlation between homocysteine, lipids and HBA1c as indicators of development of poor diabetic control.
EAS16-0414, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. SERUM APOLIPOPROTEIN B48 CONCENTRATION IS HIGH IN PATIENTS WITH IMPAIRED GLUCOSE TOLERANCE AND INCREASES AFTER ORAL GLUCOSE INGESTION IN PATIENTS WITH DIABETES MELLITUS D. Masuda. Osaka University Graduate School of Medicine, Department of Cardiovascular Medicine, Suita- Osaka, Japan Objectives: The postprandial accumulation of chylomicron remnants (CMR) is highly atherogenic and observed in patients with diabetes mellitus (DM). We evaluated whether CMR was accumulated in patients with the impaired glucose tolerance (IGT) by measuring a constituent of CMR, apolipoprotein B-48 (apoB-48) concentration, and was increased by the oral glucose load. Methods: Fasting concentrations of apoB-48 and metabolic markers were compared in normal glucose tolerance (NGT, n¼27) subjects, IGT (n¼19) and DM (n¼39) patients. And these markers were also compared in fasting and four time points after the glucose load in NGT subjects (n¼28) and DM patients (n¼57). Metabolic markers were examined for any correlation with apoB-48 concentration and a significant determinant of a high logapoB-48 level was examined by a multiple regression analysis. Results: Fasting log-apoB-48 level was significantly higher in IGT and DM groups than in NGT groups. The log-apoB-48 level was significantly correlated with apoC-3, the 2-hour insulin levels and area under the curve (AUC)-insulin. A significant determinant of a fasting high log-apoB-48 level was apoC-3 level and AUC-insulin in IGT group. After the glucose load, apoB-48 concentrations significantly increased in both groups and the incremental(i)AUC-apoB48 was significantly higher in DM group than NGT group. The iAUC-apoB-48 significantly correlated with fasting insulin, HOMA-IR and apoC2/apoC3 in DM group, while no correlation was observed in NGT group. A significant determinant of the increased iAUCapoB48 was apoC2/apoC3 in all subjects and DM group.
Conclusions: CMR is accumulated in IGT patients and increased after the glucose load, which may relate to the increased atherogenicity.
EAS16-0551, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. FASTING INSULIN LEVELS PREDICT FATAL CARDIOVASCULAR (CVD) EVENTS MORE ACCURATELY THAN ANY OTHER MARKER OF GLUCOSE METABOLISM. THE 10-YEAR FOLLOW-UP OF THE ATTICA STUDY (2002e2012) E. Georgousopoulou 1, D. Panagiotakos 1, C. Chrysohoou 2, I. Skoumas 2, A. Laskaris 1, C. Stefanadis 2, C. Pitsavos 2. 1 Harokopio University, NutritionDietetics, Athens, Greece; 2 University of Athens, Hippokration Hospital, Athens, Greece Objectives: According to latest guidelines for CVD prevention, diabetic subjects should be treated with secondary prevention guidelines, even if their medical history is free of CVD. Taken into account that the diagnosis of diabetes is given using blood glucose levels, there is still information regarding the role of hyperinsulinemia that is missing. Thus, several glucose metabolism markers might be more useful in order to identify high CVD risk subjects. Methods: from May 2001 to December 2002, 1514 men and 1528 women (>18 y) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-12, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD (coronary heart disease, acute coronary syndromes, stroke, or other CVD) was defined according to WHO-ICD-10 criteria. Results: the fasting insulin levels (mU/mL) were the most important predictor for fatal CVD (Area Under Curve (AUC) 0.762, 95% Confidence Interval (CI): 0.686, 0.838). The second most important predictor was the HOMA-IR index (AUC¼0.741, 95% CI: 0.664, 0.817), followed by fasting blood glucose levels (mg/dL) (AUC¼0.711, 95% CI: 0.631, 0.792). The results were further confirmed with separate analysis for non-diabetic subjects, where insulin levels remained the most important predictor (AUC¼0.716, 95% CI: 0.624, 0.808). Conclusions: The evaluation of fasting serum insulin levels might be useful in primary CVD prevention for all subjects (diabetic and non-diabetic), in order to better identify subjects at high fatal CVD-risk
EAS16-0610, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. ALTERATIONS OF METABOLIC PARAMETERS AND ANTIOXIDANT ENZYMES IN DIABETIC AGING FEMALE RAT BRAIN: NEUROPROTECTIVE ROLE OF METFORMIN P. Kumar 1, N. Baquer 2. 1 Jawaharlal Nehru University, New Delhi, India; 2 Jawaharlal Nehru University, School of Life Sciences, New Delhi, India Objectives: The objective of this study was to investigate beneficial effects of metformin on membrane bound enzymes (monoamine oxidase, Na+ K+ ATPse,) and antioxidant enzymes (sueroxidase dismutase, glutathione Stransferases), lipid peroxidation, neurolipofuscin, DNA degradation in diabetic aging brain of female rats. Methods: Young (3 months) adult (12 months) and aged (24 months) rats will be diabetic by using alloxan monohydrate. Metformin was administered i.p. at a dose of 200 mg/kg/day for 30 days to both control and diabetic aging rats. Learning was tested in a Morris water maze. A detailed study was carried on membrane linked enzymes, membrane fluidity, neurolipofuscin, antioxidant enzymes and DNA degradation to identify the antidiabetic and antiaging role of metformin using biochemical ,molecular and histiochemical study. Results: Present study shows that there was a similar pattern of increased lipid peroxidation, neurolipofuscin, DNA degradation and monoamine oxidase activity and a decrease in membrane fluidity, Na+ K+ ATPse, antixodant enzymes activities in brain of both aging and diabetes. Metformin
Abstracts / Atherosclerosis 252 (2016) e1ee196
MELLITUS COMPLICATIONS CORRELATION BETWEEN HOMOCYSTEINE AND TYPE-2 DIABETES MELLITUS COMPLICATIONS M. Emukhvari 1, N. Zabakhidze 1, T. M. Akhvlediani 1, Kvantaliani 2. 1 Reasearch Institute of Clinical Medicine, Laboratory of Clinical Diagnostics, Tbilisi, Georgia; 2 Medical Centre “Healing Home”, Department of Cardiology, Tbilisi, Georgia Objectives: The damage of small blood vessels(microangiopathy), medial and large arteries(macroangiopathy) in diabetic angiopathy is connected with the disorders of carbohydrate metabolism and homocysteine levels. The aim of the study was association of homocysteine , HBA1c and lipid profile levels in patients with type-2diabetes. Methods: 50 diabetic patients (age 56.2 ± 1.3)( group I) and30 healthy adults(age 52.3±1.0)( II group) were recruited. In Group I were 33 men and 17 women. Group II consisted from 16 men and 14 women. The patients with type-2 diabetes were divided into two subgroups: subgroup I e ( N¼28) patients with associated complications , and subgroup II e 22 patients without complications of diabetes. Homocysteine as well HBA1c, lipids( total cholesterol, HDL, LDL and triglicerides) and urine microalbumine parameters were measured. Results: We found that homocysteine levels are significantly higher in complicated diabetes ( 22.0±0,7 mmol/l ) than in patients without complications of diabeted(17.1±0.8 mmol/l). Significantly elevated homocysteine levels were found in patients with CAD, stroke and neuropathy as compared to control group. There was a positive correlation between homocysteine, total cholesterol, LDL andTG levels(r¼ 3.44, r¼3.67, and r¼ 4.0). The correlation between microalbuminuria and homocystein were also positive(r¼ 3.92). Conclusions: In patients who have developed micro/macro angiopathy of vessels we founded hyperhomocysteinemia with lipid profile disorders and high level of HBA1c. ..Taking into consideration the result obtained, we think it is possible to use positive correlation between homocysteine, lipids and HBA1c as indicators of development of poor diabetic control.
EAS16-0414, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. SERUM APOLIPOPROTEIN B48 CONCENTRATION IS HIGH IN PATIENTS WITH IMPAIRED GLUCOSE TOLERANCE AND INCREASES AFTER ORAL GLUCOSE INGESTION IN PATIENTS WITH DIABETES MELLITUS D. Masuda. Osaka University Graduate School of Medicine, Department of Cardiovascular Medicine, Suita- Osaka, Japan Objectives: The postprandial accumulation of chylomicron remnants (CMR) is highly atherogenic and observed in patients with diabetes mellitus (DM). We evaluated whether CMR was accumulated in patients with the impaired glucose tolerance (IGT) by measuring a constituent of CMR, apolipoprotein B-48 (apoB-48) concentration, and was increased by the oral glucose load. Methods: Fasting concentrations of apoB-48 and metabolic markers were compared in normal glucose tolerance (NGT, n¼27) subjects, IGT (n¼19) and DM (n¼39) patients. And these markers were also compared in fasting and four time points after the glucose load in NGT subjects (n¼28) and DM patients (n¼57). Metabolic markers were examined for any correlation with apoB-48 concentration and a significant determinant of a high logapoB-48 level was examined by a multiple regression analysis. Results: Fasting log-apoB-48 level was significantly higher in IGT and DM groups than in NGT groups. The log-apoB-48 level was significantly correlated with apoC-3, the 2-hour insulin levels and area under the curve (AUC)-insulin. A significant determinant of a fasting high log-apoB-48 level was apoC-3 level and AUC-insulin in IGT group. After the glucose load, apoB-48 concentrations significantly increased in both groups and the incremental(i)AUC-apoB48 was significantly higher in DM group than NGT group. The iAUC-apoB-48 significantly correlated with fasting insulin, HOMA-IR and apoC2/apoC3 in DM group, while no correlation was observed in NGT group. A significant determinant of the increased iAUCapoB48 was apoC2/apoC3 in all subjects and DM group.
Conclusions: CMR is accumulated in IGT patients and increased after the glucose load, which may relate to the increased atherogenicity.
EAS16-0551, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. FASTING INSULIN LEVELS PREDICT FATAL CARDIOVASCULAR (CVD) EVENTS MORE ACCURATELY THAN ANY OTHER MARKER OF GLUCOSE METABOLISM. THE 10-YEAR FOLLOW-UP OF THE ATTICA STUDY (2002e2012) E. Georgousopoulou 1, D. Panagiotakos 1, C. Chrysohoou 2, I. Skoumas 2, A. Laskaris 1, C. Stefanadis 2, C. Pitsavos 2. 1 Harokopio University, NutritionDietetics, Athens, Greece; 2 University of Athens, Hippokration Hospital, Athens, Greece Objectives: According to latest guidelines for CVD prevention, diabetic subjects should be treated with secondary prevention guidelines, even if their medical history is free of CVD. Taken into account that the diagnosis of diabetes is given using blood glucose levels, there is still information regarding the role of hyperinsulinemia that is missing. Thus, several glucose metabolism markers might be more useful in order to identify high CVD risk subjects. Methods: from May 2001 to December 2002, 1514 men and 1528 women (>18 y) without any clinical evidence of CVD or any other chronic disease, at baseline, living in greater Athens area, Greece, were enrolled. In 2011-12, the 10-year follow-up was performed in 2583 participants (15% of the participants were lost to follow-up). Incidence of fatal or non-fatal CVD (coronary heart disease, acute coronary syndromes, stroke, or other CVD) was defined according to WHO-ICD-10 criteria. Results: the fasting insulin levels (mU/mL) were the most important predictor for fatal CVD (Area Under Curve (AUC) 0.762, 95% Confidence Interval (CI): 0.686, 0.838). The second most important predictor was the HOMA-IR index (AUC¼0.741, 95% CI: 0.664, 0.817), followed by fasting blood glucose levels (mg/dL) (AUC¼0.711, 95% CI: 0.631, 0.792). The results were further confirmed with separate analysis for non-diabetic subjects, where insulin levels remained the most important predictor (AUC¼0.716, 95% CI: 0.624, 0.808). Conclusions: The evaluation of fasting serum insulin levels might be useful in primary CVD prevention for all subjects (diabetic and non-diabetic), in order to better identify subjects at high fatal CVD-risk
EAS16-0610, METABOLIC ABNORMALITIES AND ATHEROSCLEROSIS: DIABETES. ALTERATIONS OF METABOLIC PARAMETERS AND ANTIOXIDANT ENZYMES IN DIABETIC AGING FEMALE RAT BRAIN: NEUROPROTECTIVE ROLE OF METFORMIN P. Kumar 1, N. Baquer 2. 1 Jawaharlal Nehru University, New Delhi, India; 2 Jawaharlal Nehru University, School of Life Sciences, New Delhi, India Objectives: The objective of this study was to investigate beneficial effects of metformin on membrane bound enzymes (monoamine oxidase, Na+ K+ ATPse,) and antioxidant enzymes (sueroxidase dismutase, glutathione Stransferases), lipid peroxidation, neurolipofuscin, DNA degradation in diabetic aging brain of female rats. Methods: Young (3 months) adult (12 months) and aged (24 months) rats will be diabetic by using alloxan monohydrate. Metformin was administered i.p. at a dose of 200 mg/kg/day for 30 days to both control and diabetic aging rats. Learning was tested in a Morris water maze. A detailed study was carried on membrane linked enzymes, membrane fluidity, neurolipofuscin, antioxidant enzymes and DNA degradation to identify the antidiabetic and antiaging role of metformin using biochemical ,molecular and histiochemical study. Results: Present study shows that there was a similar pattern of increased lipid peroxidation, neurolipofuscin, DNA degradation and monoamine oxidase activity and a decrease in membrane fluidity, Na+ K+ ATPse, antixodant enzymes activities in brain of both aging and diabetes. Metformin