- Email: [email protected]
ß2-glycoprotein I complexes and leukocyte ABCA1 gene expression
S150
Abstracts
Conclusion: The results showed that reduction of 500 kcal from the daily estimated energy requirement caused a significant weight loss in both genders, especially in women. If this diet was continued for at least 3 months, the results would be quite considerable. Keywords: Diet, Obesity, Weight loss, BMI doi:10.1016/j.clinbiochem.2011.08.369
Poster – [A-10-570-1] Serum levels of oxidized LDL/ß2-glycoprotein I complexes and leukocyte ABCA1 gene expression Maryam Darabia, Mohsen Anib, Ahmad Movahedianb, Mohammad Rabbanib, Elahe Zareanb, Mojtaba Panjepourb a School Of Medicine, Qazvin University Of Medical Sciences, Qazvin, Iran b School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran E-mail addresses: [email protected] (M. Darabi), [email protected] (M. Ani), [email protected] (A. Movahedian), [email protected] (M. Rabbani), [email protected] (E. Zarean), [email protected] (M. Panjepour) Introduction: The ATP binding cassette A1 (ABCA1) is a key participant in the reverse cholesterol transport (RCT) whereby mediates cholesterol efflux directly to HDL particles. Experimental results support the hypothesis of oxidative stress association with RCT. The aim of the present study was to investigate the relationship between human leukocytes ABCA1 gene expression and oxidative stress. Materials and Methods: The population included 60 normolipidemic postmenopausal women. Leukocytes ABCA1 gene expression and total serum antioxidant capacity (TAC) and serum levels of oxLDL/ β2GPI complexes concentrations were measured. Results: Serum triglycerides concentration was significantly higher in subjects with elevated oxLDL/β2GPI levels, compared to those with lower levels (P=0.007). There was no significant relationship between oxLDL/β2GPI complexes and TAC levels and leukocytes ABCA1 gene expression even after adjustment for confounding factors. Conclusion: Despite the association between oxLDL/β2GPI levels and unfavorable lipid profile, our results did not support the hypothesis that the oxidative stress is associated with ABCA1 gene expression. Further studies are needed to address the effect of dysmetabolic abnormalities on the relationship between oxidized lipids and ABCA1 expression and activity. Keywords: ATP binding cassette A1, oxLDL/β2GPI, Oxidative stress doi:10.1016/j.clinbiochem.2011.08.370
Poster – [A-10-575-1] Review and assessment the relationship between age, sex and liver tests in patients with thyroid disorders Barani Baravati Zohreh kerman;bolvar havaniroz 90 mojtamae baran vahede5 E-mail address: [email protected] Introduction: Incidence of thyroid tissue differentiation and evolution of genes regulates general increase of this hormone causes hyperthyroidism and increased metabolic rate, along with impotence and loss of these hormones reduce fat and increase metabolism with obesity is false. Materials and Methods: This study tests the relationship between age, sex and liver in patients with hypothyroidism and hyperthyroid-
ism has been designed. Therefore, 209 clients have been studied by random and they stratified in three groups of hypothyroidism, hyperthyroidism and normal healthy volunteer as control group. Blood samples examined by laboratory testing and thyroid function tests were carried out for all cases. Results and Conclusion: The survey findings showed that the frequency of hyperthyroidism is higher in women and 64.5% of patients with hyperthyroidism were in the age group 20–40 years and 58% of patients with hypothyroidism were under 20 years, Average on test results also showed that the rate of major liver AST (SGOT) hyperthyroidism in individuals and in individuals ALT (SGPT) small work was higher than other groups. The importance of diagnosing and treating thyroid disease according to age and liver disorders that can cause mistakes and unnecessary steps and high costs will be expressed. Keywords: Hypothyroidism, Hyperthyroidism, Liver tests, Age and sex
doi:10.1016/j.clinbiochem.2011.08.371
Poster – [A-10-577-1] Comparative study on the effects of crocin, crocetin and picrocrocin on NMU-induced breast cancer in female rats Ashrafi Mahbobea, S.Z. Bathaiea, S. Abrounb, S.M. Sajjadia a Department Of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modarres University, P.O.Box: 14115–111, Tehran, Iran b Department of Hemtology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran E-mail addresses: [email protected] (A. Mahbobe), [email protected] (S.Z. Bathaie), [email protected] (S. Abroun), [email protected] (S.M. Sajjadi) Introduction: Saffron as a medicinal plant, has traditionally been used in folk remedy against many diseases. Saffron stigma, contain chemical substances such as crocin, crocetin, picrocrocin and safranal that show beneficial effects against some diseases. On the other hand breast cancer is the second leading cause of cancer related death among women and about 70% of these cancers are estrogendependent for growth. Induction of mammary tumors following the administration of NMU in rat is a preferred model of breast cancer induction in the experimental animals for investigating of breast cancer in women. These tumors are estrogen-dependent, aggressive and locally invasive, in a similar degree to that observed in the human disease. Thus, in the present research, the effects of crocin, crocetin or picrocrocin on the NMU-induced breast cancer in female rats are reported. Materials and Methods: At first, purification of the named compounds from saffron was performed. Then, breast cancer was induced by intraperitoneal injection of NMU (50 mg/kg bw) at different ages of rats. The animals were weekly weighed and palpated in order to record the number, location and size of tumors. After appearance of tumors and its suitable size (1.5 cm), treatment was began by i.p. injection of effective dose of each saffron constituents. Results: The results showed the beneficial effects of all components on cancer treatment, in order to decrease the volume of tumors, but in different degrees. The molecular mechanism of their effectiveness on cell cycle parameters is under investigation. Keywords: Breast cancer, Crocin, Crocetin, Picrocrocin, N-nitroso-methyl urea (NMU)
doi:10.1016/j.clinbiochem.2011.08.372
Abstracts
Conclusion: The results showed that reduction of 500 kcal from the daily estimated energy requirement caused a significant weight loss in both genders, especially in women. If this diet was continued for at least 3 months, the results would be quite considerable. Keywords: Diet, Obesity, Weight loss, BMI doi:10.1016/j.clinbiochem.2011.08.369
Poster – [A-10-570-1] Serum levels of oxidized LDL/ß2-glycoprotein I complexes and leukocyte ABCA1 gene expression Maryam Darabia, Mohsen Anib, Ahmad Movahedianb, Mohammad Rabbanib, Elahe Zareanb, Mojtaba Panjepourb a School Of Medicine, Qazvin University Of Medical Sciences, Qazvin, Iran b School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran E-mail addresses: [email protected] (M. Darabi), [email protected] (M. Ani), [email protected] (A. Movahedian), [email protected] (M. Rabbani), [email protected] (E. Zarean), [email protected] (M. Panjepour) Introduction: The ATP binding cassette A1 (ABCA1) is a key participant in the reverse cholesterol transport (RCT) whereby mediates cholesterol efflux directly to HDL particles. Experimental results support the hypothesis of oxidative stress association with RCT. The aim of the present study was to investigate the relationship between human leukocytes ABCA1 gene expression and oxidative stress. Materials and Methods: The population included 60 normolipidemic postmenopausal women. Leukocytes ABCA1 gene expression and total serum antioxidant capacity (TAC) and serum levels of oxLDL/ β2GPI complexes concentrations were measured. Results: Serum triglycerides concentration was significantly higher in subjects with elevated oxLDL/β2GPI levels, compared to those with lower levels (P=0.007). There was no significant relationship between oxLDL/β2GPI complexes and TAC levels and leukocytes ABCA1 gene expression even after adjustment for confounding factors. Conclusion: Despite the association between oxLDL/β2GPI levels and unfavorable lipid profile, our results did not support the hypothesis that the oxidative stress is associated with ABCA1 gene expression. Further studies are needed to address the effect of dysmetabolic abnormalities on the relationship between oxidized lipids and ABCA1 expression and activity. Keywords: ATP binding cassette A1, oxLDL/β2GPI, Oxidative stress doi:10.1016/j.clinbiochem.2011.08.370
Poster – [A-10-575-1] Review and assessment the relationship between age, sex and liver tests in patients with thyroid disorders Barani Baravati Zohreh kerman;bolvar havaniroz 90 mojtamae baran vahede5 E-mail address: [email protected] Introduction: Incidence of thyroid tissue differentiation and evolution of genes regulates general increase of this hormone causes hyperthyroidism and increased metabolic rate, along with impotence and loss of these hormones reduce fat and increase metabolism with obesity is false. Materials and Methods: This study tests the relationship between age, sex and liver in patients with hypothyroidism and hyperthyroid-
ism has been designed. Therefore, 209 clients have been studied by random and they stratified in three groups of hypothyroidism, hyperthyroidism and normal healthy volunteer as control group. Blood samples examined by laboratory testing and thyroid function tests were carried out for all cases. Results and Conclusion: The survey findings showed that the frequency of hyperthyroidism is higher in women and 64.5% of patients with hyperthyroidism were in the age group 20–40 years and 58% of patients with hypothyroidism were under 20 years, Average on test results also showed that the rate of major liver AST (SGOT) hyperthyroidism in individuals and in individuals ALT (SGPT) small work was higher than other groups. The importance of diagnosing and treating thyroid disease according to age and liver disorders that can cause mistakes and unnecessary steps and high costs will be expressed. Keywords: Hypothyroidism, Hyperthyroidism, Liver tests, Age and sex
doi:10.1016/j.clinbiochem.2011.08.371
Poster – [A-10-577-1] Comparative study on the effects of crocin, crocetin and picrocrocin on NMU-induced breast cancer in female rats Ashrafi Mahbobea, S.Z. Bathaiea, S. Abrounb, S.M. Sajjadia a Department Of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modarres University, P.O.Box: 14115–111, Tehran, Iran b Department of Hemtology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran E-mail addresses: [email protected] (A. Mahbobe), [email protected] (S.Z. Bathaie), [email protected] (S. Abroun), [email protected] (S.M. Sajjadi) Introduction: Saffron as a medicinal plant, has traditionally been used in folk remedy against many diseases. Saffron stigma, contain chemical substances such as crocin, crocetin, picrocrocin and safranal that show beneficial effects against some diseases. On the other hand breast cancer is the second leading cause of cancer related death among women and about 70% of these cancers are estrogendependent for growth. Induction of mammary tumors following the administration of NMU in rat is a preferred model of breast cancer induction in the experimental animals for investigating of breast cancer in women. These tumors are estrogen-dependent, aggressive and locally invasive, in a similar degree to that observed in the human disease. Thus, in the present research, the effects of crocin, crocetin or picrocrocin on the NMU-induced breast cancer in female rats are reported. Materials and Methods: At first, purification of the named compounds from saffron was performed. Then, breast cancer was induced by intraperitoneal injection of NMU (50 mg/kg bw) at different ages of rats. The animals were weekly weighed and palpated in order to record the number, location and size of tumors. After appearance of tumors and its suitable size (1.5 cm), treatment was began by i.p. injection of effective dose of each saffron constituents. Results: The results showed the beneficial effects of all components on cancer treatment, in order to decrease the volume of tumors, but in different degrees. The molecular mechanism of their effectiveness on cell cycle parameters is under investigation. Keywords: Breast cancer, Crocin, Crocetin, Picrocrocin, N-nitroso-methyl urea (NMU)
doi:10.1016/j.clinbiochem.2011.08.372