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Serum matrix metalloproteinase-9 levels in patients with acute myocardial infarction
Available online at www.sciencedirect.com
Clinical Biochemistry 42 (2009) 342 – 346
Abstracts
Posters: Miscellaneous C1 The effects of asymmetric dimethyl arginine and nitric oxide levels on early prognosis in patients with myocardial infarction Sukran Turkes 1, Fusun Erdenen 1, Cuneyt Muderrisoglu 1, Habibe Genc 2, Hale Aral 3, Hafize Uzun 2 1 Istanbul Training and Research Hospital, Department of Internal Medicine, Istanbul, Turkey 2 Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey 3 Istanbul Training and Research Hospital, Department of Biochemistry, Istanbul, Turkey Objectives: It is unclear whether abnormalities of nitric oxide metabolism are related to hemodynamic dysfunction and mortality in patients with acute myocardial infarction (MI). Decreased nitric oxide (NOx) and high levels of asymmetric dimethyl arginine (ADMA) are proposed independent risk factors in coronary artery disease. We investigated the relationship of NOx and ADMA levels with early prognosis in patients with MI. Design & Methods: 59 patients (9 women and 50 men, aged 32–75) who were hospitalized at Coronary Care Unit and agematched 20 healthy controls were included in the study. All clinical and laboratory parameters were monitored. Left ventricular systolic function (LVSF) was investigated by echocardiography as a prognostic marker of cardiac function. NO and ADMA levels were measured on the first day of infarction. NOx was measured spectrophotometrically at 430 nm using the Griess reaction with a commercial kit. Plasma ADMA concentrations were determined by competitive ELISA. Results: MI patients had higher plasma ADMA (0,78 ± 0.44 μmol/L) levels than controls (0.45 ± 0.2 μmol/L). NOx levels were 35.6 ± 10.4 μmol/L in MI patients and 32.5 ± 3.7 μmol/L in controls. There was not a correlation between total and LDL-cholesterol, triglyceride, hs-CRP levels, smoking history, presence of hypertension or diabetes, localization and extent of infarction and ADMA and NOx levels. Conclusion: MI patients had higher plasma ADMA levels than controls and there was not any relation with other coronary risk factors such as total and LDL-cholesterol, triglyceride, hs-CRP levels, smoking history, presence of hypertension or diabetes, localization and extent of infarction and ADMA and NOx levels. We also could not find a
correlation between ADMA, NOx levels with LVSF and early cardiac events. Keywords: Myocardial infarction; Coronary artery disease; Left ventricular systolic function (LVSF); Nitric oxide (NO); Asymmetric dimethyl arginine (ADMA) doi:10.1016/j.clinbiochem.2008.09.071
C2 Serum matrix metalloproteinase-9 levels in patients with acute myocardial infarction Savas Guzel 1, Ozden Serin 1, Guzin Yilmaz 1, Eda Celik Guzel 2, Guvenc Guvenen 3 1 Taksim Training and Research Hospital, Biochemistry Laboratory, Istanbul, Turkey 2 Taksim Training and Research Hospital, Family Practice, Istanbul, Turkey 3 Istanbul Training and Research Hospital, Biochemistry Laboratory, Istanbul, Turkey Objective: The aim of our study was to investigate the role of MMP-9 in the diagnosis of acute myocardial infarction (AMI) and compare it with the other atherosclerotic risk factors. Design & Methods: Our study was comprised of 23 AMI, 6 unstable angina pectoris (USAP) patients and 10 healthy subjects as controls. MMP-9, CK-MB and AST levels were determined in blood samples obtained at their admission to the emergency department and serially at 12th, 24th and 48th hours. Results: According to our results MMP9 levels at admission were significantly higher in AMI patients (149 ± 51.6 pg/ml) than USAP patients (102.8± 35.6 pg/ml) and the control group (69.1 ± 23.1 pg/ml) (p b 0.001, p b 0.05, respectively). MMP-9 levels at admission were significantly higher than 24th (119.7 ± 48.0 pg/ml) and 48th (111.6 ± 42.4 pg/ml) hours (p b 0.05). Conclusion: According to our results MMP-9 increase pattern might be a potential follow up marker in AMI. Keywords: Matrix metalloproteinase-9; Myocardial infarction; Unstable angina pectoris; Arteriosclerosis; Inflammation doi:10.1016/j.clinbiochem.2008.09.072
Clinical Biochemistry 42 (2009) 342 – 346
Abstracts
Posters: Miscellaneous C1 The effects of asymmetric dimethyl arginine and nitric oxide levels on early prognosis in patients with myocardial infarction Sukran Turkes 1, Fusun Erdenen 1, Cuneyt Muderrisoglu 1, Habibe Genc 2, Hale Aral 3, Hafize Uzun 2 1 Istanbul Training and Research Hospital, Department of Internal Medicine, Istanbul, Turkey 2 Istanbul University, Cerrahpasa Medical Faculty, Department of Biochemistry, Istanbul, Turkey 3 Istanbul Training and Research Hospital, Department of Biochemistry, Istanbul, Turkey Objectives: It is unclear whether abnormalities of nitric oxide metabolism are related to hemodynamic dysfunction and mortality in patients with acute myocardial infarction (MI). Decreased nitric oxide (NOx) and high levels of asymmetric dimethyl arginine (ADMA) are proposed independent risk factors in coronary artery disease. We investigated the relationship of NOx and ADMA levels with early prognosis in patients with MI. Design & Methods: 59 patients (9 women and 50 men, aged 32–75) who were hospitalized at Coronary Care Unit and agematched 20 healthy controls were included in the study. All clinical and laboratory parameters were monitored. Left ventricular systolic function (LVSF) was investigated by echocardiography as a prognostic marker of cardiac function. NO and ADMA levels were measured on the first day of infarction. NOx was measured spectrophotometrically at 430 nm using the Griess reaction with a commercial kit. Plasma ADMA concentrations were determined by competitive ELISA. Results: MI patients had higher plasma ADMA (0,78 ± 0.44 μmol/L) levels than controls (0.45 ± 0.2 μmol/L). NOx levels were 35.6 ± 10.4 μmol/L in MI patients and 32.5 ± 3.7 μmol/L in controls. There was not a correlation between total and LDL-cholesterol, triglyceride, hs-CRP levels, smoking history, presence of hypertension or diabetes, localization and extent of infarction and ADMA and NOx levels. Conclusion: MI patients had higher plasma ADMA levels than controls and there was not any relation with other coronary risk factors such as total and LDL-cholesterol, triglyceride, hs-CRP levels, smoking history, presence of hypertension or diabetes, localization and extent of infarction and ADMA and NOx levels. We also could not find a
correlation between ADMA, NOx levels with LVSF and early cardiac events. Keywords: Myocardial infarction; Coronary artery disease; Left ventricular systolic function (LVSF); Nitric oxide (NO); Asymmetric dimethyl arginine (ADMA) doi:10.1016/j.clinbiochem.2008.09.071
C2 Serum matrix metalloproteinase-9 levels in patients with acute myocardial infarction Savas Guzel 1, Ozden Serin 1, Guzin Yilmaz 1, Eda Celik Guzel 2, Guvenc Guvenen 3 1 Taksim Training and Research Hospital, Biochemistry Laboratory, Istanbul, Turkey 2 Taksim Training and Research Hospital, Family Practice, Istanbul, Turkey 3 Istanbul Training and Research Hospital, Biochemistry Laboratory, Istanbul, Turkey Objective: The aim of our study was to investigate the role of MMP-9 in the diagnosis of acute myocardial infarction (AMI) and compare it with the other atherosclerotic risk factors. Design & Methods: Our study was comprised of 23 AMI, 6 unstable angina pectoris (USAP) patients and 10 healthy subjects as controls. MMP-9, CK-MB and AST levels were determined in blood samples obtained at their admission to the emergency department and serially at 12th, 24th and 48th hours. Results: According to our results MMP9 levels at admission were significantly higher in AMI patients (149 ± 51.6 pg/ml) than USAP patients (102.8± 35.6 pg/ml) and the control group (69.1 ± 23.1 pg/ml) (p b 0.001, p b 0.05, respectively). MMP-9 levels at admission were significantly higher than 24th (119.7 ± 48.0 pg/ml) and 48th (111.6 ± 42.4 pg/ml) hours (p b 0.05). Conclusion: According to our results MMP-9 increase pattern might be a potential follow up marker in AMI. Keywords: Matrix metalloproteinase-9; Myocardial infarction; Unstable angina pectoris; Arteriosclerosis; Inflammation doi:10.1016/j.clinbiochem.2008.09.072