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Serum phospholipide analysis by chromatography and infrared spectrophotometry
ABSTRACTS
601
A rapid and sensitive spectrophotometric method for the assay of chymotrypsin, C. J. Martin, J. Golubow, and A. E. Axelrod, J. Biol. Chem., 234, 294-98 (1959). A sensitive method for the determination of chymotrypsin activity with quantities of enzyme as small as 4.5 mpg. is described. The thyroxine-binding protein of bovine synovial fluid, 0. W. Neuhaus and V. Sogoian, J. Riol. Chem., 234, 821-23, (1959). The protein-bound iodine for normal bovine synovial fluid was found to be about twice that of serum, 2.6 pg. of iodine per grain. Biosynthesis of ribose and deoxyribose in Escherichia coli, F. Bagatell, E. Wright, and H. Sable, J. Biol. Chem., 234, 1369-73 (1959). Sodium acetate was the source of carbon for the synthesis of ribose and deoxyribose in E. coli grown in a synthetic medium. Serum phospholipide analysis by chromatography and infrared spectrophotometry, G. Nelson and N. Freeman, J. Biol. Chem., 234, 1375-80, (1959). A semimicro method has been developed for the analysis of serum phospholipides by the use of chromatography and infrared spectrophotometry. Separation of fatty acids from tubercle bacillus by gas chromatography: identi$cotion of oleic acid, J. Coson and P. Tavs, J. Bio2. Chem., 234, 1401-1405 (1959). The position of esters previously termed the &.-CI9 fraction has been examined by use of gas phase chromatography. The fractionation of cholesterol esters by silicic acid chromatography, P. Klein and E. Janssen, J. Biol. Chem., 234, 1417-20, (1959). The method offers a consistent, accurate means of resolving biological mixtures of cholesterol esters into their principal components. Physical and chemical studies of a limited reaction of iodine with proteins, L. Cnnningham and B. Nuenke, J. Biol. Chem., 234, 1447-51 (1959). A spectrophotometric method for the estimation of sulfhydryl groups based on the extinction of triiodide ion at 355 rnF is described. Fluorescence studies of coenzyme-binding to beef heart and lactic dehydrogenase, S. Shifrin, N. Kaplan, and M. Ciotti, J. Biol. Chem., 234, 1555-62 (1959). A comparison of the fluorescence properties of reduced pyridine nucleotides and the 3-acetylpyridine analogue of reduced diphosphopyridine nucleotide (DPNH) is presented. Photochemical determination of the oxidases of bacteria, L. Castor and B. Chance, J. BioZ. Chem., 234, 1587-92, (1959). On the basis of photochemical action spectra of respiration inhibited by carbon monoxide, four bacterial pigments are demonstrated to be terminal respiratory enzymes. Chromatographic procedure for the determination of urinary corticosteroids and Cle steroids, H. Wilson, J. J. Borris, and M. M. Garrison, J. Clin. Endocrinol. and
601
A rapid and sensitive spectrophotometric method for the assay of chymotrypsin, C. J. Martin, J. Golubow, and A. E. Axelrod, J. Biol. Chem., 234, 294-98 (1959). A sensitive method for the determination of chymotrypsin activity with quantities of enzyme as small as 4.5 mpg. is described. The thyroxine-binding protein of bovine synovial fluid, 0. W. Neuhaus and V. Sogoian, J. Riol. Chem., 234, 821-23, (1959). The protein-bound iodine for normal bovine synovial fluid was found to be about twice that of serum, 2.6 pg. of iodine per grain. Biosynthesis of ribose and deoxyribose in Escherichia coli, F. Bagatell, E. Wright, and H. Sable, J. Biol. Chem., 234, 1369-73 (1959). Sodium acetate was the source of carbon for the synthesis of ribose and deoxyribose in E. coli grown in a synthetic medium. Serum phospholipide analysis by chromatography and infrared spectrophotometry, G. Nelson and N. Freeman, J. Biol. Chem., 234, 1375-80, (1959). A semimicro method has been developed for the analysis of serum phospholipides by the use of chromatography and infrared spectrophotometry. Separation of fatty acids from tubercle bacillus by gas chromatography: identi$cotion of oleic acid, J. Coson and P. Tavs, J. Bio2. Chem., 234, 1401-1405 (1959). The position of esters previously termed the &.-CI9 fraction has been examined by use of gas phase chromatography. The fractionation of cholesterol esters by silicic acid chromatography, P. Klein and E. Janssen, J. Biol. Chem., 234, 1417-20, (1959). The method offers a consistent, accurate means of resolving biological mixtures of cholesterol esters into their principal components. Physical and chemical studies of a limited reaction of iodine with proteins, L. Cnnningham and B. Nuenke, J. Biol. Chem., 234, 1447-51 (1959). A spectrophotometric method for the estimation of sulfhydryl groups based on the extinction of triiodide ion at 355 rnF is described. Fluorescence studies of coenzyme-binding to beef heart and lactic dehydrogenase, S. Shifrin, N. Kaplan, and M. Ciotti, J. Biol. Chem., 234, 1555-62 (1959). A comparison of the fluorescence properties of reduced pyridine nucleotides and the 3-acetylpyridine analogue of reduced diphosphopyridine nucleotide (DPNH) is presented. Photochemical determination of the oxidases of bacteria, L. Castor and B. Chance, J. BioZ. Chem., 234, 1587-92, (1959). On the basis of photochemical action spectra of respiration inhibited by carbon monoxide, four bacterial pigments are demonstrated to be terminal respiratory enzymes. Chromatographic procedure for the determination of urinary corticosteroids and Cle steroids, H. Wilson, J. J. Borris, and M. M. Garrison, J. Clin. Endocrinol. and