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Significance and association of human papilloma virus infection with notch signaling pathway in ovarian cancer
Abstracts / Journal of the Anatomical Society of India 65S (2016) S1–S97
279 Significance and association of human papilloma virus infection with notch signaling pathway in ovarian cancer G. Rath 1 , Poonam Jawanjal 1,∗ , A. Batra 2 , U. Sharma 3 1 Department of Anatomy, VMMC & Safdarjung Hospital, New Delhi, India 2 Department of Obstetrics and Gynecology, VMMC & Safdarjung Hospital, New Delhi, India 3 Department of Pathology, VMMC & Safdarjung Hospital, New Delhi, India
Aims and objectives: Human papilloma virus (HPV) is the precursor for activation of Notch signaling in various epithelial cancers but its involvement in ovarian tumors is still barely understood. Hence, this study was aimed to determine the prevalence of HPV16/18 infection and its role in regulation of Notch pathway in ovarian epithelial carcinoma. Material and methods: Surgically resected tissue samples collected from non-neoplastic controls (NNC; n = 65), borderline ovarian tumors (BOT; n = 29) and invasive epithelial carcinoma (EOC; n = 88) were processed for HPV-16/18 genotyping and expression of Notch pathway proteins (Jagged-1, DLL-1, Hes-1 and Cyclin D1) by multiplex PCR as well as immunohistochemical analysis. Results: The PCR analysis revealed, HPV-16 positivity in 13.7% of BOTs and 18.1% of EOCs. HPV-18 infection was present only in 3.4% (3/88) of EOCs. On the immunohistochemical study, cytoplasmic overexpression of Jagged-1 in 41.3% of BOTs and 35.2% of EOCs was noticed, while its nuclear positivity was significantly increased in 51.7% of BOTs and 44.3% of EOCs as compared to NNC (p = 0.0001). Similarly, 51.7% (15/29) and 48.2% (14/29) of BOTs as well as 31.8% (28/88) and 47.7% (42/88) of EOCs also demonstrated up-regulation of DLL-1 in cytoplasm and nucleus respectively. (p = 0.0001; p = 0.0001). Furthermore, nuclear immunostaining of Hes-1 and Cyclin D1 proteins was up-regulated both in BOTs and EOCs. Significant positive association of HPV-16 with Notch proteins in BOTs and EOCs. Conclusion: The significant positivity of HPV and its association with notch effector proteins demonstrate the role of HPV mediated deregulation of notch signaling in the pathogenesis of ovarian cancer. Conflicts of interest The authors have none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.287 280 Development of the frontal cerebral cortex in human foetuses: A histological study Prerna Jagdish ∗ , S.L. Jethani, Singh Deepa Himalayan Institute of Medical Sciences, Jollygrant, Dehradun, Uttarakhand, India Aims and objectives: The aim of the present research is to study the development of the frontal cortex of human fetuses histologically with reference to its laminar architecture at varying weeks of gestation. Material and methods: Skulls of 50 fetuses of known gestational age were dissected to obtain cerebral hemispheres. An H&E
S91
and a Cresyl violet stained slide was prepared from frontal lobe (precentral gyrus) for better appreciation of the cellular zones. Laminar architecture was identified and thickness of each layer was determined along with the total average cortical thickness. Results: The average cortical thickness of frontal lobe increased with the increasing weeks of gestation. Subcortical plate appeared by the 12th week of gestation and was well appreciated in the midgestational period. The intermediate and the subventricular zones merged towards the later weeks of gestation. Conclusion: All the layers showed an increase in thickness with increasing weeks of gestation and a histologically distinct laminar architecture was observed by the full term. Conflicts of interest The authors have none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.288 281 Histological changes in human placentae at different stages of maturity Sudhakarbabu Chiruthoti Sri Venkateswara Medical College, Tirupati 517507, India Aims and objectives: The placental examination provides significant information related to intrauterine foetal death, intrauterine growth retardation, malformations, infections and effects of maternal diseases on fetal growth. The magnitude of the clinical problems related to the development and the functions of the placenta is so vast that it worth to undertake histological aspect of human placenta. A thorough examination of placenta is neglected often underestimated by Physician, Gynecologist, Pediatrician and Pathologist in spite of its invaluable role in the fetal development. A sound knowledge of normal structure and development is highly essential to appreciate the pathological changes. Material and methods: Here I am presenting histological findings of 25 placentas at different gestational ages present study was conducted in the Department of Anatomy Narayana Medical College Nellore in collaboration with the Department of Obstetrics and Gynecology. Small placental bits of fresh tissue of various gestational ages were collected from the labour room subjected for processing for histological studies. Results and conclusion: It was observed that in 10 cases of 6–12 weeks of gestation the histological observation of double layered trophoblast, large cytotrophoblastic cells with large round nucleus, marginally thick syncytiotrophoblast cells with small round nucleus, numerous Hofbauer cell, centrally placed numerous fetal blood vessels. In 10 cases of gestational period between 13-24 weeks histological findings were increased number of villi, gradually thinned syncytiotrophoblast, Large villous capillaries, Hofbauer cells show vacuolated appearance, in 5 cases between 25 weeks to full term numerous villi, reduced stroma, numerous syncytial knots. Conflicts of interest The author has none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.289
279 Significance and association of human papilloma virus infection with notch signaling pathway in ovarian cancer G. Rath 1 , Poonam Jawanjal 1,∗ , A. Batra 2 , U. Sharma 3 1 Department of Anatomy, VMMC & Safdarjung Hospital, New Delhi, India 2 Department of Obstetrics and Gynecology, VMMC & Safdarjung Hospital, New Delhi, India 3 Department of Pathology, VMMC & Safdarjung Hospital, New Delhi, India
Aims and objectives: Human papilloma virus (HPV) is the precursor for activation of Notch signaling in various epithelial cancers but its involvement in ovarian tumors is still barely understood. Hence, this study was aimed to determine the prevalence of HPV16/18 infection and its role in regulation of Notch pathway in ovarian epithelial carcinoma. Material and methods: Surgically resected tissue samples collected from non-neoplastic controls (NNC; n = 65), borderline ovarian tumors (BOT; n = 29) and invasive epithelial carcinoma (EOC; n = 88) were processed for HPV-16/18 genotyping and expression of Notch pathway proteins (Jagged-1, DLL-1, Hes-1 and Cyclin D1) by multiplex PCR as well as immunohistochemical analysis. Results: The PCR analysis revealed, HPV-16 positivity in 13.7% of BOTs and 18.1% of EOCs. HPV-18 infection was present only in 3.4% (3/88) of EOCs. On the immunohistochemical study, cytoplasmic overexpression of Jagged-1 in 41.3% of BOTs and 35.2% of EOCs was noticed, while its nuclear positivity was significantly increased in 51.7% of BOTs and 44.3% of EOCs as compared to NNC (p = 0.0001). Similarly, 51.7% (15/29) and 48.2% (14/29) of BOTs as well as 31.8% (28/88) and 47.7% (42/88) of EOCs also demonstrated up-regulation of DLL-1 in cytoplasm and nucleus respectively. (p = 0.0001; p = 0.0001). Furthermore, nuclear immunostaining of Hes-1 and Cyclin D1 proteins was up-regulated both in BOTs and EOCs. Significant positive association of HPV-16 with Notch proteins in BOTs and EOCs. Conclusion: The significant positivity of HPV and its association with notch effector proteins demonstrate the role of HPV mediated deregulation of notch signaling in the pathogenesis of ovarian cancer. Conflicts of interest The authors have none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.287 280 Development of the frontal cerebral cortex in human foetuses: A histological study Prerna Jagdish ∗ , S.L. Jethani, Singh Deepa Himalayan Institute of Medical Sciences, Jollygrant, Dehradun, Uttarakhand, India Aims and objectives: The aim of the present research is to study the development of the frontal cortex of human fetuses histologically with reference to its laminar architecture at varying weeks of gestation. Material and methods: Skulls of 50 fetuses of known gestational age were dissected to obtain cerebral hemispheres. An H&E
S91
and a Cresyl violet stained slide was prepared from frontal lobe (precentral gyrus) for better appreciation of the cellular zones. Laminar architecture was identified and thickness of each layer was determined along with the total average cortical thickness. Results: The average cortical thickness of frontal lobe increased with the increasing weeks of gestation. Subcortical plate appeared by the 12th week of gestation and was well appreciated in the midgestational period. The intermediate and the subventricular zones merged towards the later weeks of gestation. Conclusion: All the layers showed an increase in thickness with increasing weeks of gestation and a histologically distinct laminar architecture was observed by the full term. Conflicts of interest The authors have none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.288 281 Histological changes in human placentae at different stages of maturity Sudhakarbabu Chiruthoti Sri Venkateswara Medical College, Tirupati 517507, India Aims and objectives: The placental examination provides significant information related to intrauterine foetal death, intrauterine growth retardation, malformations, infections and effects of maternal diseases on fetal growth. The magnitude of the clinical problems related to the development and the functions of the placenta is so vast that it worth to undertake histological aspect of human placenta. A thorough examination of placenta is neglected often underestimated by Physician, Gynecologist, Pediatrician and Pathologist in spite of its invaluable role in the fetal development. A sound knowledge of normal structure and development is highly essential to appreciate the pathological changes. Material and methods: Here I am presenting histological findings of 25 placentas at different gestational ages present study was conducted in the Department of Anatomy Narayana Medical College Nellore in collaboration with the Department of Obstetrics and Gynecology. Small placental bits of fresh tissue of various gestational ages were collected from the labour room subjected for processing for histological studies. Results and conclusion: It was observed that in 10 cases of 6–12 weeks of gestation the histological observation of double layered trophoblast, large cytotrophoblastic cells with large round nucleus, marginally thick syncytiotrophoblast cells with small round nucleus, numerous Hofbauer cell, centrally placed numerous fetal blood vessels. In 10 cases of gestational period between 13-24 weeks histological findings were increased number of villi, gradually thinned syncytiotrophoblast, Large villous capillaries, Hofbauer cells show vacuolated appearance, in 5 cases between 25 weeks to full term numerous villi, reduced stroma, numerous syncytial knots. Conflicts of interest The author has none to declare. http://dx.doi.org/10.1016/j.jasi.2016.08.289