Silk fibroin and poly-caprolactone vascular grafts tested in vitro and in vivo mimic natural vessel

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E-Poster Presentation

Pneumomediastinum and subcutaneous emphysema after periodontal treatment using air-flow equipment: a case report K.H. Kim ∗ , J.W. Cho, J.H. Lee, C.H. Kim, J.U. Park Catholic University of Korea, St. Mary’s Hospital, Seoul, Republic of Korea Case report: Subcutaneous emphysema is a rare but serious side effect of dental and oral surgery procedures. It is characterized by air being forced underneath the tissue, leading to swelling, crepitus on palpation, and with potential to spread along the fascial planes to the periorbital, mediastinal, pericardial, and/or thoracic spaces. A wide range of causes has been documented for the origin of subcutaneous emphysema during dental treatment including: crown preparations, endodontic therapy, extractions, as well as oral surgery procedures. The patient was a 58-year-old woman who presented to the Seoul St. Mary’s hospital emergency department with a chief complaint of facial edema, dyspnea and chest discomfort after periodontal treatment using an air-flow equipment in local dental clinic. Oxygen therapy and intravenous injection of steroid and antihistamine were done. It was noted that the patient had pain and swelling on left lower molar region, pus discharging on same site. Severe edema was observed on periorbital region to neck with heatness. An audible crepitus sound was heard during palpation. Antibiotic therapy was done, as symptom suggesting dental abscess is observed. After injection of antibiotics, symptom was relieved, but she felt chest discomfort continuously. CT scan with contrast depicted confluent and extensive soft tissue emphysematous changes involving face and deep neck spaces and pneumomediastinum. The patient was referred to thoracic surgery department, oxygen therapy was decided continuously. After 10 h, patient’s chief complaint was resolved. After 1 week, all symptoms disappeared and follow-up neck CT scan finding was disappearance of edema and pneumomediastinum. http://dx.doi.org/10.1016/j.ijom.2015.08.188 Silk fibroin and poly-caprolactone vascular grafts tested in vitro and in vivo mimic natural vessel S.G. Kim 3,∗ , A.R. Park 1 , Y.H. Park 1 , H.J. Kim 2 , M.K. Kim 3 , H.Y. Kweon 4 , S.C. Kundu 5 1

Seoul National University, Seoul, Republic of Korea Seoul National University Dental Hospital, Seoul, Republic of Korea 3 Gangneung-Wonju National University, Gangneung, Republic of Korea 4 National Academy of Agricultural Science, Jeonju, Republic of Korea 5 Indian Institute of Technology Kharagpur (IIT), West Bengal, India 2

Background: The replacement therapy with autograft for the damaged vessel has been tried. However, the available donor vessel is limited. Objectives: The objective of this study was the development of small diameter artificial vessel. Methods: A tri-layered nano-fiber scaffold of silk fibroin (SF) and poly-␧-caprolactone (PCL) composed of inner, middle, and outer layer is fabricated using sequential electrospinning method. The inner layer is composed of SF with Spirulina extract. The anti-thrombic effect of Spirulina extract is tested first. The structure, mechanical property, and cytocompatibility of scaffold are

evaluated. The tri-layered nano-fiber scaffold is implanted into rat carotid artery and the sample after 3 weeks implantation is evaluated for histological analysis. Findings and conclusions: SF with Spirulina extract show the inhibition of platelet adhesion. The tri-layered scaffold with middle layer of fibroin/PCL blend show the excellent longitudinal and circumferential tensile strength, burst pressure strength, and suture retention strength. Carotid arteries maintain vessel patency until 3 weeks after implantation. The inner lumen of the scaffold show regenerated endothelial cells. The results show that tri-layered tubular type SF/PCL vascular grafts can be used in vascular tissue engineering due to their excellent mechanical property and good tissue regeneration. http://dx.doi.org/10.1016/j.ijom.2015.08.189 Physico-chemical properties of hydroxyapatite synthesized from the shell of abalone, Haliotis sp. J.S. Kim 1,∗ , S.G. Kim 2 1

Pre-Dentistry, Republic of Korea Chosun University, Department of Oral and Maxillofacial Surgery, School of Dentistry, Gwangju, Republic of Korea

2

Objective: To evaluate the physico-chemical properties of hydroxyapatite (HA) synthesized from the shell of abalone, Haliotis sp. to use as a bone grafting bioceramic. Methods: Ceramic materials were synthesized from the shell of abalone, Haliotis sp. collected from regional sea-food market by typically developed synthesis process, which was composed of washing step, sequential synthesis of CaO and CaCO3 , phosphorylation using phosphoric acid, and finally sintering process at 1230 ◦ C. And chemical composition of HA synthesized from abalone shell was verified by Energy Dispersive Spectrometry (EDS), X-ray Diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). To verify the biological safety, MTT and Cell live & Dead assay were performed in MG-63 cells incubated with the effluent of ceramic materials synthesized from abalone shell for 24 h. Furthermore, alkaline phosphatase (ALP) assay and alizrine Red staining were performed in MG-63 cells incubated with HA synthesized from abalone shell. Results: The results of EDS showed that the ceramic material synthesized from abalone shell was composed of O, P and Ca. Furthermore, the picks obtained in the range of 500–4000 on the FT-IR showed that the synthesized ceramic material had an identical position compared with commercial HA used as positive control. Moreover, the crystal structure of the synthesized ceramic material on the XRD had an identical position compared with commercial HA. Both MTT and Cell live & Dead assay to verify the biological safety showed that the cell cytotoxicity did not observe in the MG-63 cells incubated with the effluent of ceramic material synthesized from abalone shell for 24 h. Although the ALP activity of ceramic material synthesized from abalone shell was higher than commercial HA, but there was no significance. The results of Alizarin Red staining showed that the mineralizing property of ceramic material synthesized from abalone shell had a similar with commercial HA. Conclusion: Our results such as EDS, FT-IR and XRD are strongly indicating that ceramic material which is synthesized from the developed novel synthesis process using abalone shell is a HA. Furthermore, both MTT and Cell Live & Dead assay results are showing that the HA synthesized from abalone shell is a ceramic material with biological safety. Taken together, HA synthesized from abalone shell might be had a similar