- Email: [email protected]
Simple bone cyst in the right branch of mandible
OOOO Volume 114, Number 4 SQUAMOUS CELL CARCINOMA Agostini M1,2, Bastos DC1, Seguin F1, Zecchin KG1, Raposo HF3, Coletta RD1, Graner E1 - 1STATE UNIVERSITY OF CAMPINAS (UNICAMP), SCHOOL OF DENTISTRY OF PIRACICABA - DEPARTMENT OF ORAL DIAGNOSIS, 2FEDERAL UNIVERSITY OF RIO DE JANEIRO (UFRJ), SCHOOL OF DENTISTRY - DEPARTMENT OF ORAL DIAGNOSIS AND PATHOLOGY, 3STATE UNIVERSITY OF CAMPINAS (UNICAMP), INSTITUTE OF BIOLOGY - DEPARTMENT OF PHYSIOLOGY AND BIOPHYSICS Background/Objective: This study aimed to investigate whether the inhibition of the lipogenic enzyme fatty acid synthase (FASN) with orlistat could impact oral squamous cell carcinoma (OSCC) metastatic spread. Study design: We developed an orthotropic model of OSCC through the implantation of SCC-9 ZsGreen LN-1 cells into the tongue of BALB/c nude mice, which generate orthotropic tumors that spontaneously metastasize to the cervical lymph nodes. Twenty four hours after implantation the animals started to be treated with orlistat or its vehicle. Results: Inhibition of FASN activity with orlistat or specific siRNAs significantly reduces the proliferation and promotes apoptosis in SCC-9 cells in vitro. Mice treated with orlistat showed a decrease in the volume (p⬍ 0.001) and proliferation indexes (p⬍ 0.05) of the primary tumors and had 43% less metastatic lymph nodes than the controls (p⬍ 0.01). Conclusion: FASN may be a potential molecular target for the chemotherapy treatment of OSCC patients.
A FIBROUS DYSPLASIA CHARACTERIZATION BASED ON LACUNARITY ANALYSIS Cordeiro MS1, Durighetto Jr. AF2, Backes AR3, Gonçalves EG4, Oliveira JX1 - 1UNIVERSIDADE DE SÃO PAULO - DIAGNÓSTICO BUCAL, 2 UNIVERSIDADE DE UBERLÂNDIA - DIAGNÓSTICO BUCAL, 3UNIVERSIDADE DE UBERLÂNDIA - FACULDADE DE COMPUTAÇÃO, 4UNIVERSIDADE DE UBERLÂNDIA FACULDADE DE MEDICINA Background: Fibrous dysplasia (FD) is a developmental anomaly in which the normal medullary space of the affected bone is replaced by fibro-osseous tissue. The lacunarity analysis, have enabled to quantify objectively the visual attributes obtained during the process of image analysis. The aim of this study was to characterize the fibrous dysplasia by analyzing the lacunarity. Study design: We evaluated 10 patients by lacunarity analysis, used an axial and coronal view CT and then were selected the regions of interest in the areas of dysplastic and contralateral normal bone by means of MATLAB® software. Results: The lacunarity was lower in the areas of dysplastic region in relation to normal bone, namely the first presented a more uniform texture than the second. Thus, the cross-validation technique leave-one-out was possible to separate the groups with high accuracy (94.75%). Conclusion: The lacunarity is a contributory method of image analysis to characterize the FD.
SIMPLE BONE CYST IN THE RIGHT BRANCH OF MANDIBLE Alves MGO1, Caris AR, Carvalho BFC, Santos NC, Prado RF, Cavalcante ASR, Almeida JD - 1UNIV. ESTADUAL PAULISTA - BIOCIÊNCIAS E DIAGNÓSTICO BUCAL
ORAL ABSTRACT Abstracts e127 Simple bone cyst is a benign cavity in the bone empty or containing fluid that is free of lining epithelium. The location is exclusively mandibular and the most frequent is the second premolar and symphysis region. An 16-year-old white girl seek for the outpatient clinic with no pain complaints, referred by orthodontists who detected through routine radiography image a radiolucent area in the right branch of the mandible. On clinical examination there was no change; computed tomography showed a lytic lesion with regular contours and homogeneous. Puncture was unproductive and during incisional biopsy it was observed an empty cavity with smooth and bright walls. Based on surgical findings, the differential diagnosis was simple bone cyst. Histopathologic examination revealed a fragment of tissue showing inflammation and adjacent neoformed bone trabeculae, compatible with simple bone cyst. The patient is examined frequently and bone formation is evident.
ORAL INFLAMMATORY MIOFIBROBLASTIC TUMOR: AN UNUSUAL CASE REPORT Barbosa NG1, Oliveira PT2, Medeiros AMC1, Queiroz LMG1, Carvalho MG3, Gurgel AC3 - 1UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE - PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA ORAL, 2UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE, 3UNIVERSIDADE POTIGUAR Inflammatory Myofibroblastic Tumor (IMT) is an uncommon benign lesion, with unknown etiology, composed of myofibroblasts cells and varying numbers of inflammatory cells. We present the case of a 12-year-old boy with pedunculated soft tissue painless nodule located on lingual surface of left alveolar ridge next to teeth numbers 36 and 37 with 3 months evolution. There was no bleeding in the area. The clinical diagnosis was peripheral giant cells. Panoramic radiography showed alveolar bone resorption. An incisional biopsy was performed and the histopathological examination showed fibroblastic or myofibroblastic plump or spindle cells in fibrous vascular background with an infiltrate of chronic inflammatory cells. Diagnosis was IMT and the patient underwent enucleation of the lesion. The case continues under follow-up and has shown no evidence of recurrence. As IMT in oral cavity may be confused with malignant tumors, it is important to recognize the distinction among them to provide adequate treatment.
HYPOHIDROTIC ECTODERMAL DYSPLASIA Dias NNO1, Junqueira MA1, Carli ML1, Pedreira FRO1, De Oliveira DSB1, Hanemann JAC1 - 1ALFENAS FEDERAL UNIVERSITY - CLINIC AND SURGERY/ STOMATOLOGY A 8 year-old male patient, attended to the Stomatology Clinic of UNIFAL-MG, complaining about the absence of many teeth. He was diagnosed with hypohidrotic ectodermal dysplasia. The diagnosis had been made at the age of 10 months. The hair was blond, sparse and thin, there was a reduced density of eyelashes and eyebrows and protuberance of lips and periocular hyperpigmentation. Intraoral examination showed the absence of all lower teeth and of several superiors. The teeth present had microdontia and all the anterior were conical in shape. After the diagnosis, extraction of the 52 and 62 teeth, an orthodontic removable appliance space maintainer and a denture lower were made. After the eruption of the maxillary central incisors conical in shape, they were reconstructed with resin composite. The patient is still being treated, and currently, he is using a remov-
A FIBROUS DYSPLASIA CHARACTERIZATION BASED ON LACUNARITY ANALYSIS Cordeiro MS1, Durighetto Jr. AF2, Backes AR3, Gonçalves EG4, Oliveira JX1 - 1UNIVERSIDADE DE SÃO PAULO - DIAGNÓSTICO BUCAL, 2 UNIVERSIDADE DE UBERLÂNDIA - DIAGNÓSTICO BUCAL, 3UNIVERSIDADE DE UBERLÂNDIA - FACULDADE DE COMPUTAÇÃO, 4UNIVERSIDADE DE UBERLÂNDIA FACULDADE DE MEDICINA Background: Fibrous dysplasia (FD) is a developmental anomaly in which the normal medullary space of the affected bone is replaced by fibro-osseous tissue. The lacunarity analysis, have enabled to quantify objectively the visual attributes obtained during the process of image analysis. The aim of this study was to characterize the fibrous dysplasia by analyzing the lacunarity. Study design: We evaluated 10 patients by lacunarity analysis, used an axial and coronal view CT and then were selected the regions of interest in the areas of dysplastic and contralateral normal bone by means of MATLAB® software. Results: The lacunarity was lower in the areas of dysplastic region in relation to normal bone, namely the first presented a more uniform texture than the second. Thus, the cross-validation technique leave-one-out was possible to separate the groups with high accuracy (94.75%). Conclusion: The lacunarity is a contributory method of image analysis to characterize the FD.
SIMPLE BONE CYST IN THE RIGHT BRANCH OF MANDIBLE Alves MGO1, Caris AR, Carvalho BFC, Santos NC, Prado RF, Cavalcante ASR, Almeida JD - 1UNIV. ESTADUAL PAULISTA - BIOCIÊNCIAS E DIAGNÓSTICO BUCAL
ORAL ABSTRACT Abstracts e127 Simple bone cyst is a benign cavity in the bone empty or containing fluid that is free of lining epithelium. The location is exclusively mandibular and the most frequent is the second premolar and symphysis region. An 16-year-old white girl seek for the outpatient clinic with no pain complaints, referred by orthodontists who detected through routine radiography image a radiolucent area in the right branch of the mandible. On clinical examination there was no change; computed tomography showed a lytic lesion with regular contours and homogeneous. Puncture was unproductive and during incisional biopsy it was observed an empty cavity with smooth and bright walls. Based on surgical findings, the differential diagnosis was simple bone cyst. Histopathologic examination revealed a fragment of tissue showing inflammation and adjacent neoformed bone trabeculae, compatible with simple bone cyst. The patient is examined frequently and bone formation is evident.
ORAL INFLAMMATORY MIOFIBROBLASTIC TUMOR: AN UNUSUAL CASE REPORT Barbosa NG1, Oliveira PT2, Medeiros AMC1, Queiroz LMG1, Carvalho MG3, Gurgel AC3 - 1UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE - PROGRAMA DE PÓS-GRADUAÇÃO EM PATOLOGIA ORAL, 2UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE, 3UNIVERSIDADE POTIGUAR Inflammatory Myofibroblastic Tumor (IMT) is an uncommon benign lesion, with unknown etiology, composed of myofibroblasts cells and varying numbers of inflammatory cells. We present the case of a 12-year-old boy with pedunculated soft tissue painless nodule located on lingual surface of left alveolar ridge next to teeth numbers 36 and 37 with 3 months evolution. There was no bleeding in the area. The clinical diagnosis was peripheral giant cells. Panoramic radiography showed alveolar bone resorption. An incisional biopsy was performed and the histopathological examination showed fibroblastic or myofibroblastic plump or spindle cells in fibrous vascular background with an infiltrate of chronic inflammatory cells. Diagnosis was IMT and the patient underwent enucleation of the lesion. The case continues under follow-up and has shown no evidence of recurrence. As IMT in oral cavity may be confused with malignant tumors, it is important to recognize the distinction among them to provide adequate treatment.
HYPOHIDROTIC ECTODERMAL DYSPLASIA Dias NNO1, Junqueira MA1, Carli ML1, Pedreira FRO1, De Oliveira DSB1, Hanemann JAC1 - 1ALFENAS FEDERAL UNIVERSITY - CLINIC AND SURGERY/ STOMATOLOGY A 8 year-old male patient, attended to the Stomatology Clinic of UNIFAL-MG, complaining about the absence of many teeth. He was diagnosed with hypohidrotic ectodermal dysplasia. The diagnosis had been made at the age of 10 months. The hair was blond, sparse and thin, there was a reduced density of eyelashes and eyebrows and protuberance of lips and periocular hyperpigmentation. Intraoral examination showed the absence of all lower teeth and of several superiors. The teeth present had microdontia and all the anterior were conical in shape. After the diagnosis, extraction of the 52 and 62 teeth, an orthodontic removable appliance space maintainer and a denture lower were made. After the eruption of the maxillary central incisors conical in shape, they were reconstructed with resin composite. The patient is still being treated, and currently, he is using a remov-