- Email: [email protected]
Single and combined effects of exposure concentration and duration on chromium phytoaccumulation
Abstracts / Current Opinion in Biotechnology 22S (2011) S15–S152
S145
P56
P58
Evaluation of puroindoline b as a house keeping gene in wild wheat species
Single and combined effects of exposure concentration and duration on chromium phytoaccumulation
Erdogan Esref Hakki 1 , Songul Uygan 1 , Mehmet Hamurcu 2 , Sait Gezgin 2 , Buket Serife Bozkurt 3 , Seyit Ali Kayis 4
Fatih Duman, Fatih Dogan Koca, Ahmet Aksoy
1
Selcuk University, Konya, Turkey 2 Selcuk University, Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Konya, Turkey 3 Selcuk University, Faculty of Dentistry, Konya, Turkey 4 Selcuk University, Faculty of Agriculture, Department of Animal Science, Konya, Turkey
Department of Biology, Faculty of Sciences, Erciyes University, Kayseri, Turkey E-mail address: [email protected] (A. Aksoy)
Aim was to evaluate puroindoline b as a housekeeping gene (HKG) to be used in the normalization of gene expression in the wild wheat species. Ct values of real-time PCR amplifications were data to evaluate. Samples used were obtained from the plants grown in hydrophonic system under different levels of boric acid treatments (0, 25, 250, and 500 ppm). Plant samples were taken in different time intervals of plant growth. Evaluation was made by using comparison of treatment group means via One-way ANOVA in each time period. Results show that there are no statistically significantly differences between the treatment groups within each time interval. Puroindoline b can be used in the normalization of gene expression in the wild wheat species as a HKG.
Although the metal accumulation characteristics of some aquatic plants have been intensely studied, we have insufficient information about the effects of exposure concentration and duration on Cr accumulation. In this study, Ceratophyllum demersum L. samples were exposed to 0, 1, 2, 5 and 10 mM of CrCl3 (Cr III) and K2 Cr2 O7 (Cr VI) for 1, 2, 4 and 7 days to evaluate the effects of Cr concentration and exposure duration. The accumulation of both Cr species increased with concentration and duration dependently. The highest accumulation of Cr (14.26 mg g−1 dw) was found in plants exposed to 10 mM of Cr (VI) for 7 days. There was a significant interactive effect of concentration and duration on Cr accumulation for Cr(III) exposure. Conversely, it was not seen significant concentration–duration interaction for Cr(VI) exposure. Results also showed that the effect of concentration was higher than exposure duration on the Cr accumulation for both Cr(III) and Cr(VI) applications. Finding of this study may be useful for phytoremediation of water polluted with Cr.
doi:10.1016/j.copbio.2011.05.480
doi:10.1016/j.copbio.2011.05.482
E-mail address: [email protected] (E.E. Hakki)
P57 The effects of toxic level boron treatments on the biochemical features of Puccinellia distans Mehmet Hamurcu 1 , Tijen Demiral 2 , Sait Caglar 1 , Veysi Kizmaz 2 , Erdogan Esref Hakki 1
Gezgin 1 , Hayriye
1
Faculty of Agriculture, Selcuk University, Konya, Turkey Faculty of Science and Literature, Harran University, Sanliurfa, Turkey 2
E-mail address: [email protected] (T. Demiral) Aim was to determine the antioxidant defence system response of Puccinellia distans (Jacq) Parl under boron stress P. distans seedlings were grown for 30 days in half-strength Hoagland solution before boron (B) treatments with 0.05 (control), 25, 250 and 500 mg kg−1 in the form of boric acid. Plants were harvested on the 30th and 60th days after treatments. Relative water content (RWC), B concentration and activities of the antioxidant enzymes and their isoenzymatic profiles and malondialdehyte contents were measured. Highest plant growth rate was observed in 25 mg kg−1 B treated plants. B concentration in the shoots of P. distans increased significantly with increasing levels of applied B. Relative water contents of P. distans samples were increased with high dosages of B treatments. Significant increases in activities of some antioxidant enzymes (SOD, CAT and APX) were observed in shoots of P. distans given 25, 250 and 500 mg kg−1 B. B tolerance of P. distans might be closely related to increased capacity of the antioxidative system to scavenge reactive oxygen species and thus suppress lipid peroxidation under B stress. doi:10.1016/j.copbio.2011.05.481
Renewables, Biorefinery, Bioenergy, Biofuels and Bioproducts Section Q1 Bioprocess and agroprocess intensifications Galip Akay 1,2,3 1
Process Intensification and Miniaturisation Centre, School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK 2 The Institute of Stem Cell Biology and Regenerative Medicine, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK 3 Institute for Nanoscale Science and Technology, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK E-mail address: [email protected] One of the impacts of global warming and reduced fossil fuel resources is the emergence of Food, Energy and Water shortages (FEWs). Technologies in agriculture, biochemical, chemical, energy and environmental processes can be intensified to circumvent FEWs in the coming decades through the replacement of oil and petrochemical refineries by Biorefineries utilising biomass as feedstock while avoiding the impact of biomass generation on food and water sources. In this review of author’s recent research in Agro-Process Intensification (A-PI) and BioProcess Intensification (B-PI), the background phenomenon based on the behaviour of micro-organisms and cells within microenvironment is described with examples from tissue engineering and biotechnology. B-PI in various systems ranges from 5 to 200 fold compared with the existing technology. The principles of B-PI are applied to achieve A-PI. Grass was used to understand the mechanism of A-PI. Enhancement of plant yield was above 200% under water stress conditions. Pea plant was used to
S145
P56
P58
Evaluation of puroindoline b as a house keeping gene in wild wheat species
Single and combined effects of exposure concentration and duration on chromium phytoaccumulation
Erdogan Esref Hakki 1 , Songul Uygan 1 , Mehmet Hamurcu 2 , Sait Gezgin 2 , Buket Serife Bozkurt 3 , Seyit Ali Kayis 4
Fatih Duman, Fatih Dogan Koca, Ahmet Aksoy
1
Selcuk University, Konya, Turkey 2 Selcuk University, Faculty of Agriculture, Department of Soil Science and Plant Nutrition, Konya, Turkey 3 Selcuk University, Faculty of Dentistry, Konya, Turkey 4 Selcuk University, Faculty of Agriculture, Department of Animal Science, Konya, Turkey
Department of Biology, Faculty of Sciences, Erciyes University, Kayseri, Turkey E-mail address: [email protected] (A. Aksoy)
Aim was to evaluate puroindoline b as a housekeeping gene (HKG) to be used in the normalization of gene expression in the wild wheat species. Ct values of real-time PCR amplifications were data to evaluate. Samples used were obtained from the plants grown in hydrophonic system under different levels of boric acid treatments (0, 25, 250, and 500 ppm). Plant samples were taken in different time intervals of plant growth. Evaluation was made by using comparison of treatment group means via One-way ANOVA in each time period. Results show that there are no statistically significantly differences between the treatment groups within each time interval. Puroindoline b can be used in the normalization of gene expression in the wild wheat species as a HKG.
Although the metal accumulation characteristics of some aquatic plants have been intensely studied, we have insufficient information about the effects of exposure concentration and duration on Cr accumulation. In this study, Ceratophyllum demersum L. samples were exposed to 0, 1, 2, 5 and 10 mM of CrCl3 (Cr III) and K2 Cr2 O7 (Cr VI) for 1, 2, 4 and 7 days to evaluate the effects of Cr concentration and exposure duration. The accumulation of both Cr species increased with concentration and duration dependently. The highest accumulation of Cr (14.26 mg g−1 dw) was found in plants exposed to 10 mM of Cr (VI) for 7 days. There was a significant interactive effect of concentration and duration on Cr accumulation for Cr(III) exposure. Conversely, it was not seen significant concentration–duration interaction for Cr(VI) exposure. Results also showed that the effect of concentration was higher than exposure duration on the Cr accumulation for both Cr(III) and Cr(VI) applications. Finding of this study may be useful for phytoremediation of water polluted with Cr.
doi:10.1016/j.copbio.2011.05.480
doi:10.1016/j.copbio.2011.05.482
E-mail address: [email protected] (E.E. Hakki)
P57 The effects of toxic level boron treatments on the biochemical features of Puccinellia distans Mehmet Hamurcu 1 , Tijen Demiral 2 , Sait Caglar 1 , Veysi Kizmaz 2 , Erdogan Esref Hakki 1
Gezgin 1 , Hayriye
1
Faculty of Agriculture, Selcuk University, Konya, Turkey Faculty of Science and Literature, Harran University, Sanliurfa, Turkey 2
E-mail address: [email protected] (T. Demiral) Aim was to determine the antioxidant defence system response of Puccinellia distans (Jacq) Parl under boron stress P. distans seedlings were grown for 30 days in half-strength Hoagland solution before boron (B) treatments with 0.05 (control), 25, 250 and 500 mg kg−1 in the form of boric acid. Plants were harvested on the 30th and 60th days after treatments. Relative water content (RWC), B concentration and activities of the antioxidant enzymes and their isoenzymatic profiles and malondialdehyte contents were measured. Highest plant growth rate was observed in 25 mg kg−1 B treated plants. B concentration in the shoots of P. distans increased significantly with increasing levels of applied B. Relative water contents of P. distans samples were increased with high dosages of B treatments. Significant increases in activities of some antioxidant enzymes (SOD, CAT and APX) were observed in shoots of P. distans given 25, 250 and 500 mg kg−1 B. B tolerance of P. distans might be closely related to increased capacity of the antioxidative system to scavenge reactive oxygen species and thus suppress lipid peroxidation under B stress. doi:10.1016/j.copbio.2011.05.481
Renewables, Biorefinery, Bioenergy, Biofuels and Bioproducts Section Q1 Bioprocess and agroprocess intensifications Galip Akay 1,2,3 1
Process Intensification and Miniaturisation Centre, School of Chemical Engineering and Advanced Materials, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK 2 The Institute of Stem Cell Biology and Regenerative Medicine, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK 3 Institute for Nanoscale Science and Technology, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK E-mail address: [email protected] One of the impacts of global warming and reduced fossil fuel resources is the emergence of Food, Energy and Water shortages (FEWs). Technologies in agriculture, biochemical, chemical, energy and environmental processes can be intensified to circumvent FEWs in the coming decades through the replacement of oil and petrochemical refineries by Biorefineries utilising biomass as feedstock while avoiding the impact of biomass generation on food and water sources. In this review of author’s recent research in Agro-Process Intensification (A-PI) and BioProcess Intensification (B-PI), the background phenomenon based on the behaviour of micro-organisms and cells within microenvironment is described with examples from tissue engineering and biotechnology. B-PI in various systems ranges from 5 to 200 fold compared with the existing technology. The principles of B-PI are applied to achieve A-PI. Grass was used to understand the mechanism of A-PI. Enhancement of plant yield was above 200% under water stress conditions. Pea plant was used to