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Size-Exclusion Stationary Phases
Appendix 6: Size-Exclusion Stationary Phases APPENDIX 6a
Silica-Based SEC Commercial Packings (for Aqueous SEC or GFC) Particle Size
Chemistry on Silica
Phase Name (Supplier)
Pore Size Designation
Range of Use
BioSuite
125, 250
5000e150,000
10, 12, and d 17 mm
Ultrahydrogel
120, 250, 500, 1000, 2000 Å
5000e7,000,000
6 mm
Shodex Protein KW (Showa Denko)
800 Series
1000e10,000,000
5 mm
TSK-gel SW, SWXL , SuperSW (Tosoh)
Various G2000SW, G3000SW, G3000SWXL ,UP-SW3000
Various 5000e70,000
2 (for UP), Diol 5, 8, and 10 mm
TSK-gel QC-PAK
TSK 200, TSK 300
5000e50,000
5 mm
Diol
TSK-gel BioAssist DS
125, 250, 300, 450
Various
Various: 2e15 mm
Diol
Zorbax GF Ser. (Agilent/ Crawford Scientific)
GF-250, GF-450
4000e400,000, 10,000e900,000
4 and 6 mm
Zr clad diol
Zorbax PSM (Agilent/ Thomas Scientific)
PMS 60, PMS 60S, PMS 300, PMS 1000, etc.
Various
5 mm
Silanized
LiChrosphere Si
Si60, Si100
Also normal phase 5, 10 mm
UltraSpherogel (Grace/ Beckman)
2000, 4000, etc.
d
5 mm
Bio-Sil, Bio-Select (Bio-Rad) SEC-125-5, 250-5, 400-5
1000e1,000,000
5, 10 mm
Silica
Protein-Pak (Waters)
300SW
1000e20,000
10 mm
Diol
Bio-Sep (Phenomenex)
SEC-S2000, SEC-S3000, SEC-S4000
500e20,000,000
5 mm
Hydrophilic bonded silica
SynChropak (Lab Unlimited)
GPC Peptide, GPC 100 to GPC 4000, Various ranges CATSEC 100 to 4000
5, 10 mm
Diol
543
d Diol; fully porous silica
Silica Polyether
544 APPENDIX 6b
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
Several Commercially Available Phases Used in Nonaqueous SEC (GPC)
Phase Name (Supplier)
Pore Size Designation
Range of Mw of Usea
Jordi Labs GPC DVB Fluorinated
Various from 100 to 100,000 Å
Various between 100 and 10,000,000
5 mm
PLgel (Polymer Lab. Ltd./ Agilent)
Various from 50 Å to 1,000,000 Å
Various from 100e2000 to 100,000e20,000,000
3, 5, 10, and 20 mm
PLgel multipore bed
Various from MIXED-A to MIXED-E
Various from 200e400,000 to 1000e40,000,000
5, 10, 20 mm
PolySep (Phenomenex)
Various from 1000 to 6000 and linear
Various from 100e2000 to 100,000e20,000,000
5, 10, and 20 mm
Shodex K and KF,KD, KL (Showa Denko)
Various indicated as 801, 802, 807
Various from 70,000 average to 200,000,000 average
Nominally 7 mm
Shodex K multipore bed
Various indicated as 803L, to 807L
Various from 1500 average to 200,000,000 average
Various depending on range (6, 10, and 17 mm)
TSK-Gel HXL, SuperHZ (Toyo Soda)
Various indicated as G1000 to G7000
Various from 1000 average to 400,000,000 average
Various depending on range (5e9 mm)
TSK-GEL HHR and HXL (Aldrich)
G1000H to G7000H, GMH-H, GMH-L, GMH-M
Various from 1500 to 1,000,000
5 and 13 mm
TSK-Gel HXL, SuperHZ multipore bed
GMHXL, GMHXL-HT, GMHXL-L
400,000,000 average
9, 13, and 6 mm
Styragel HR (Waters)
Various indicated as HR 0.5, HR 1 to HR 4
Various from smalle1000 to 5000e600,000
5 mm
Styragel HR multipore bed
HR 4E HR 5E
50e10,000 2000e4,000,000
5 mm
Styragel HT, Ultrastyragel (Waters)
Various indicated as HT 3 to HT 6, Ultrastyragel
Various from 500e30,000 to 200,000e10,000,000
10 mm
Styragel HMW (Waters)
HMW 7
500,000e100,000,000
20 mm
Styragel HT, Styragel HMW multipore bed
HT 6E HMW 6E
5000e10,000,000
10, 20 mm
Bio-beades, S-X beads Bio-Rad
d
400e14,000
Soft gel
Hydrocell (Biochrom Labs.)
GPC 3000, 3000HS
20,000e1,000,000
5 mm
Various
5, 10, and 20 mm
Phenogel (Phenomenex)
a
3
50 Å, 100 Å, 500, 10 Å, 104 Å, 105 Å, 106 Å, and linear
The range of Mw used is based on polystyrene as analyte.
Particle Size
545
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
APPENDIX 6c
Several Commercially Available Phases Used in Aqueous or Mixed Polar Solvents SEC (GFC)
Phase Name (Supplier)
Pore Size Designation
Range of Use (Da)
Particle Size
Chemistry
Jordi BGR
100, 500, 10 , 10 , and 105 Å
Various polymers between 102107
5 mm
PSDVBa functionalized
Jordi Hydroxylated DVB
100, 500, 103, 104 and 105 Å
Smaller molecules, not proteins
5 mm
PSDVB functionalized
Jordi polar Pac Wax
100, 500, 103, 104 and 105 Å
Various polyhydroxy 102107
5 mm
PSDVB functionalized
PL aquagel-OH (Polymer Lab. Ltd./Agilent
AOH 30, 40, 50, 60
100,000, 1,000,000, 20,000,000
5, 8 and 15 mm
Polyhydroxyl surface
PL aquagel-OH mixed bed
AOH mixed H, mixed M
20,000,000, up to 600,000 respectively
8 mm
Polymer
PolarGel
M, L
Up to 500,000
5, 10, and 20 mm
d
Shodex OHpak (Showa Denko), Protein KW
Various indicated as KB-802, to KB-806, KB-80M, SB-401, etc.
Various from 4000e20,000,000
Various
HPMMAb
TSK-GEL PW, PWXL, PWXL-CP (Toyo Soda)
Various G1000 to G6000, GM, PWXL G5000PW, GMPW, etc.
Various from 1000e8,000,000
Various
HPMMA
TSK-GEL HHR and HXL (Aldrich)
G1000H to G7000H, GMH-H, GMH-L, GMH-M
Various from 1500e1,000,000
7 mm
HPMMA
Toyopearl HW
40S, 40F, 40C, 50S, 50F, 55S, 55F, 65S, 65F, 75F (five pore sizes)
100e50,000,000
20e40 Å, 30e60 Å, 50e100 Å
HPMAc
TSK-GEL Alpha and Super AW
Alpha-3000, Alpha-5000, Super AW2500 to Super AW6000
Various up to 10,000,000
Various
HPMMA
TSK-GEL Super-Multipore PW
PW-N, PW-M, PW-H
300e50,000 to 1000e10,000,000
4, 5, and 8 mm
HPMMA
Ultrahydrogel (Waters)
Various 120 Å to 2000 Å indicated as 250, 500, etc., linear
Various from 5000e7,000,000
10 mm
HPMMA
Asahipak (Asahi Chemical/Phenomenex)
Various GS-220, GS-320, GS520, GS-620, GS-710, GFA-30, GFA-7M, GF ser.
Various from 3000e10,000,000
Various
PVAd copolymer
Asahipak (Phenomenex)
GF-310 HQ to GF710 HQ, and multimode
Various
5, 6 and 9 mm
PVA copolymer
3
4
(Continued)
546 APPENDIX 6c
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
Several Commercially Available Phases Used in Aqueous or Mixed Polar Solvents SEC (GFC)dcont’d
Phase Name (Supplier)
Pore Size Designation
Range of Use (Da)
Particle Size
Chemistry
Suprema (PSS)
30 Å, 100 Å, 300 Å, 1000 Å, Linear S, M, XL
Various from 20,000e10,000,000
Various
HPMA
MCI Gel CQP (Mitsubishi Chemical)
Various indicated as CQO06, CQP06 G, CQP10, CQP30, etc.
Various from 1000e1,000,000
10, 30 mm
HPMA
Polyhydroxyethyl aspartamide; Polyhydroxyethyl A (PolyLC Inc.)
200, 1000 Å
100e1,000,000
3 mm
d
Bio-Prep SE (Bio-Rad)
SE100/17, SE1000/17
5000e1,000,000
17 mm
Agarose
PolySep-GFC-P (Phenomenex)
1000 to 6000 and Linear
Various ranges
d
Highly hydrophilic
PSDVB ¼ Polystyrenedivinylbenzene. HPPMA ¼ Hydroxylated poly(methylmethacrylate). c HPMA ¼ Hydroxylated poly(methacrylate). d PVA ¼ Polyvinyl alcohol. a
b
APPENDIX 6d
Several More Recent SEC Columns
Column Name
Manufacturer
Phase
Particle Size
Type
AdvancedBio SEC
Agilent
Proprietary
2.7
Coreeshell
Yarra 1.8mm SEC-X150
Phenomenex
Proprietary
1.8
Porous
OHpak LB-803 and LB-806M
Shodex
Proprietary
6, 13 (LB-806M)
Polymeric
TSKGel UP-SW3000
Tosoh Bioscience
Diol
2
Porous
Silica-Based SEC Commercial Packings (for Aqueous SEC or GFC) Particle Size
Chemistry on Silica
Phase Name (Supplier)
Pore Size Designation
Range of Use
BioSuite
125, 250
5000e150,000
10, 12, and d 17 mm
Ultrahydrogel
120, 250, 500, 1000, 2000 Å
5000e7,000,000
6 mm
Shodex Protein KW (Showa Denko)
800 Series
1000e10,000,000
5 mm
TSK-gel SW, SWXL , SuperSW (Tosoh)
Various G2000SW, G3000SW, G3000SWXL ,UP-SW3000
Various 5000e70,000
2 (for UP), Diol 5, 8, and 10 mm
TSK-gel QC-PAK
TSK 200, TSK 300
5000e50,000
5 mm
Diol
TSK-gel BioAssist DS
125, 250, 300, 450
Various
Various: 2e15 mm
Diol
Zorbax GF Ser. (Agilent/ Crawford Scientific)
GF-250, GF-450
4000e400,000, 10,000e900,000
4 and 6 mm
Zr clad diol
Zorbax PSM (Agilent/ Thomas Scientific)
PMS 60, PMS 60S, PMS 300, PMS 1000, etc.
Various
5 mm
Silanized
LiChrosphere Si
Si60, Si100
Also normal phase 5, 10 mm
UltraSpherogel (Grace/ Beckman)
2000, 4000, etc.
d
5 mm
Bio-Sil, Bio-Select (Bio-Rad) SEC-125-5, 250-5, 400-5
1000e1,000,000
5, 10 mm
Silica
Protein-Pak (Waters)
300SW
1000e20,000
10 mm
Diol
Bio-Sep (Phenomenex)
SEC-S2000, SEC-S3000, SEC-S4000
500e20,000,000
5 mm
Hydrophilic bonded silica
SynChropak (Lab Unlimited)
GPC Peptide, GPC 100 to GPC 4000, Various ranges CATSEC 100 to 4000
5, 10 mm
Diol
543
d Diol; fully porous silica
Silica Polyether
544 APPENDIX 6b
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
Several Commercially Available Phases Used in Nonaqueous SEC (GPC)
Phase Name (Supplier)
Pore Size Designation
Range of Mw of Usea
Jordi Labs GPC DVB Fluorinated
Various from 100 to 100,000 Å
Various between 100 and 10,000,000
5 mm
PLgel (Polymer Lab. Ltd./ Agilent)
Various from 50 Å to 1,000,000 Å
Various from 100e2000 to 100,000e20,000,000
3, 5, 10, and 20 mm
PLgel multipore bed
Various from MIXED-A to MIXED-E
Various from 200e400,000 to 1000e40,000,000
5, 10, 20 mm
PolySep (Phenomenex)
Various from 1000 to 6000 and linear
Various from 100e2000 to 100,000e20,000,000
5, 10, and 20 mm
Shodex K and KF,KD, KL (Showa Denko)
Various indicated as 801, 802, 807
Various from 70,000 average to 200,000,000 average
Nominally 7 mm
Shodex K multipore bed
Various indicated as 803L, to 807L
Various from 1500 average to 200,000,000 average
Various depending on range (6, 10, and 17 mm)
TSK-Gel HXL, SuperHZ (Toyo Soda)
Various indicated as G1000 to G7000
Various from 1000 average to 400,000,000 average
Various depending on range (5e9 mm)
TSK-GEL HHR and HXL (Aldrich)
G1000H to G7000H, GMH-H, GMH-L, GMH-M
Various from 1500 to 1,000,000
5 and 13 mm
TSK-Gel HXL, SuperHZ multipore bed
GMHXL, GMHXL-HT, GMHXL-L
400,000,000 average
9, 13, and 6 mm
Styragel HR (Waters)
Various indicated as HR 0.5, HR 1 to HR 4
Various from smalle1000 to 5000e600,000
5 mm
Styragel HR multipore bed
HR 4E HR 5E
50e10,000 2000e4,000,000
5 mm
Styragel HT, Ultrastyragel (Waters)
Various indicated as HT 3 to HT 6, Ultrastyragel
Various from 500e30,000 to 200,000e10,000,000
10 mm
Styragel HMW (Waters)
HMW 7
500,000e100,000,000
20 mm
Styragel HT, Styragel HMW multipore bed
HT 6E HMW 6E
5000e10,000,000
10, 20 mm
Bio-beades, S-X beads Bio-Rad
d
400e14,000
Soft gel
Hydrocell (Biochrom Labs.)
GPC 3000, 3000HS
20,000e1,000,000
5 mm
Various
5, 10, and 20 mm
Phenogel (Phenomenex)
a
3
50 Å, 100 Å, 500, 10 Å, 104 Å, 105 Å, 106 Å, and linear
The range of Mw used is based on polystyrene as analyte.
Particle Size
545
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
APPENDIX 6c
Several Commercially Available Phases Used in Aqueous or Mixed Polar Solvents SEC (GFC)
Phase Name (Supplier)
Pore Size Designation
Range of Use (Da)
Particle Size
Chemistry
Jordi BGR
100, 500, 10 , 10 , and 105 Å
Various polymers between 102107
5 mm
PSDVBa functionalized
Jordi Hydroxylated DVB
100, 500, 103, 104 and 105 Å
Smaller molecules, not proteins
5 mm
PSDVB functionalized
Jordi polar Pac Wax
100, 500, 103, 104 and 105 Å
Various polyhydroxy 102107
5 mm
PSDVB functionalized
PL aquagel-OH (Polymer Lab. Ltd./Agilent
AOH 30, 40, 50, 60
100,000, 1,000,000, 20,000,000
5, 8 and 15 mm
Polyhydroxyl surface
PL aquagel-OH mixed bed
AOH mixed H, mixed M
20,000,000, up to 600,000 respectively
8 mm
Polymer
PolarGel
M, L
Up to 500,000
5, 10, and 20 mm
d
Shodex OHpak (Showa Denko), Protein KW
Various indicated as KB-802, to KB-806, KB-80M, SB-401, etc.
Various from 4000e20,000,000
Various
HPMMAb
TSK-GEL PW, PWXL, PWXL-CP (Toyo Soda)
Various G1000 to G6000, GM, PWXL G5000PW, GMPW, etc.
Various from 1000e8,000,000
Various
HPMMA
TSK-GEL HHR and HXL (Aldrich)
G1000H to G7000H, GMH-H, GMH-L, GMH-M
Various from 1500e1,000,000
7 mm
HPMMA
Toyopearl HW
40S, 40F, 40C, 50S, 50F, 55S, 55F, 65S, 65F, 75F (five pore sizes)
100e50,000,000
20e40 Å, 30e60 Å, 50e100 Å
HPMAc
TSK-GEL Alpha and Super AW
Alpha-3000, Alpha-5000, Super AW2500 to Super AW6000
Various up to 10,000,000
Various
HPMMA
TSK-GEL Super-Multipore PW
PW-N, PW-M, PW-H
300e50,000 to 1000e10,000,000
4, 5, and 8 mm
HPMMA
Ultrahydrogel (Waters)
Various 120 Å to 2000 Å indicated as 250, 500, etc., linear
Various from 5000e7,000,000
10 mm
HPMMA
Asahipak (Asahi Chemical/Phenomenex)
Various GS-220, GS-320, GS520, GS-620, GS-710, GFA-30, GFA-7M, GF ser.
Various from 3000e10,000,000
Various
PVAd copolymer
Asahipak (Phenomenex)
GF-310 HQ to GF710 HQ, and multimode
Various
5, 6 and 9 mm
PVA copolymer
3
4
(Continued)
546 APPENDIX 6c
APPENDIX 6: SIZE-EXCLUSION STATIONARY PHASES
Several Commercially Available Phases Used in Aqueous or Mixed Polar Solvents SEC (GFC)dcont’d
Phase Name (Supplier)
Pore Size Designation
Range of Use (Da)
Particle Size
Chemistry
Suprema (PSS)
30 Å, 100 Å, 300 Å, 1000 Å, Linear S, M, XL
Various from 20,000e10,000,000
Various
HPMA
MCI Gel CQP (Mitsubishi Chemical)
Various indicated as CQO06, CQP06 G, CQP10, CQP30, etc.
Various from 1000e1,000,000
10, 30 mm
HPMA
Polyhydroxyethyl aspartamide; Polyhydroxyethyl A (PolyLC Inc.)
200, 1000 Å
100e1,000,000
3 mm
d
Bio-Prep SE (Bio-Rad)
SE100/17, SE1000/17
5000e1,000,000
17 mm
Agarose
PolySep-GFC-P (Phenomenex)
1000 to 6000 and Linear
Various ranges
d
Highly hydrophilic
PSDVB ¼ Polystyrenedivinylbenzene. HPPMA ¼ Hydroxylated poly(methylmethacrylate). c HPMA ¼ Hydroxylated poly(methacrylate). d PVA ¼ Polyvinyl alcohol. a
b
APPENDIX 6d
Several More Recent SEC Columns
Column Name
Manufacturer
Phase
Particle Size
Type
AdvancedBio SEC
Agilent
Proprietary
2.7
Coreeshell
Yarra 1.8mm SEC-X150
Phenomenex
Proprietary
1.8
Porous
OHpak LB-803 and LB-806M
Shodex
Proprietary
6, 13 (LB-806M)
Polymeric
TSKGel UP-SW3000
Tosoh Bioscience
Diol
2
Porous