- Email: [email protected]
Skin Inflammation and Oxidative Stress
S70
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Keywords: Skin inflammation oxidative stress antioxidants
Enhanced oxidative stress is related to enhanced inflammation. The behavior of endogenous antioxidants in inflammatory phenomena is not quite clear. In a trial to elucidate the relation between skin inflammation, oxidative stress and antioxidants in vivo and in vitro data will be discussed. Using keratinocytes and fibroblasts producing oxidative stress either by UV light or xanthine – xanthine oxidase or tobacco smoke, the protection of antioxidants is evaluated. In vivo, in hairless mice, oxidative stress is estimated under different conditions as acute and chronic exposure to UV light or to tobacco smoke in both normal and diabetic skin, evaluating inflammation, oxidative stress and endogenous antioxidants. It seems that the dose of administered antioxidants is much important as a light pro-oxidant activity could be beneficial for the regeneration and protection of skin. In vivo, after UV light exposure, endogenous antioxidants as glutathion at the level of stratum corneum decreased while uric acid is increased. Administration of exogenous non antioxidant substances as lactic acid could also increase uric acid. Stratum corneum is much more susceptible to oxidative stress and antioxidant alterations in relation to living epidermis and dermis.
stress. SIRT3 knockdown could be a therapeutic strategy for colon cancer, since it improves the effectiveness of cytotoxic treatments. E-mail address: [email protected] (M. Torrens-Mas) Acknowledgements
Funding was provided by ISCIII (PI14/01434), MECD (FPU07042). http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.238
P-154
Diabetic skin and UV light: protection by Pinus halepensis pine bark extract Aggeliki Dimaki 1, Maria Kyriazi 1, Ioannis Sfiniadakis 2, Georgios Papaioannou 1, Lykourgos Klamarias 1, Vasileios Roussis 1, Michail Rallis 1 1 2
National and Kapodistrian University of Athens, Greece Naval Hospital of Athens, Greece
E-mail address: [email protected] (M. Rallis) Keywords: Diabetes mellitus; skin; UVR; pinus halepensis; vitamin C
http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.237
P-153
SIRT3 knockdown increases oxidative stress and sensitivity to cytotoxic treatments in SW620 cancer cells Margalida Torrens-Mas, Reyniel Hernández-López, Jorge Sastre-Serra, Pilar Roca, Jordi Oliver Grupo Multidisciplinar de Oncología Traslacional, Universitat de les Illes Balears, Institut Universitari d´Investigació en Ciències de la Salut (IUNICS), Instituto de Investigación Sanitaria de las Islas Baleares (IdISBa), Palma, Illes Balears, Spain; Ciber Fisiopatología Obesidad y Nutrición (CB06/03) Instituto Salud Carlos III, Madrid, Spain Keywords: Sirtuin 3; colon cancer; oxidative stress; oxaliplatin
Sirtuin 3 (SIRT3) is the major deacetylase in mitochondria and is activated under oxidative stress conditions. SIRT3 regulates mitochondrial metabolism and the antioxidant response to lower reactive oxygen species (ROS) production. Thus, SIRT3 could allow cells to counteract the effect of anticancer therapies, which increase ROS levels. The aim of this study was to determine whether SIRT3 knockdown in colon cancer cells could increase oxidative stress and therefore make them more sensitive to cytotoxic treatments. A stable SIRT3 silencing in SW620 human cell line was achieved through a specific shRNA. Expression of antioxidant enzymes and mitochondrial proteins was analyzed, as well as protein levels by Western Blot and MnSOD enzymatic activity. Cells were also treated with oxaliplatin to study ROS production by Amplex Reds. SIRT3 silencing resulted in a decrease of both antioxidant enzymes and proteins related to mitochondrial function, as well as a reduction in MnSOD activity. Furthermore, SIRT3 knockdown produced a significant increase in ROS production, which was greater with oxaliplatin treatment. Overall, these results suggest that SIRT3 is a key factor regulating mitochondria under oxidative
Diabetic skin is characterized by great sensitivity when compared to normal skin. Ultraviolet radiation is directly related to the oxidative stress affecting the skin. Diabetic skin, when compared to the normal one, is greatly damaged by ultraviolet radiation resulting in significant photoaging. Despite the fact that the longterm effect of UV light on normal skin has been partially investigated, for the diabetic skin data is missing. Antioxidants, such as Pinus halepensis seem to prevent photoaging. On the skin of hairless male diabetic mice the effects of UV radiation and the possible protection of Pinus halepensis pine bark extract topical application were studied, showing the significant antioxidant role of pine. E-mail address: [email protected] (M. Kyriazi) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.239
P-155
The role of aging and senescence on pancreatic β-cell function and proliferation Richard Kehm 1,2, Oliver Kluth 1,2, Annette Schürmann 1,2, Tilman Grune 1,2,3, Annika Höhn 1,2 1
German Institute of Human Nutrition (DIfE), Germany German Center for Diabetes Research (DZD), Germany 3 German Center for Cardiovascular Research (DZHK), Germany 2
Keywords: Aging; senescence; pancreatic β-cell; type-2-diabetes
Aging and age-related diseases are associated with the impairment of tissue function and their regenerative capacity. In terms of pancreatic β-cells, it is well known that insulin resistance in peripheral tissues increases with age due to metabolic changes caused by adiposity or physical inactivity. But in the presence of functional β-cells, insulin resistance alone is insufficient to lead to type-2-diabetes. However, in combination with an insulin
D. Rossin et al. / Free Radical Biology and Medicine 108 (2017) S18–S107
Keywords: Skin inflammation oxidative stress antioxidants
Enhanced oxidative stress is related to enhanced inflammation. The behavior of endogenous antioxidants in inflammatory phenomena is not quite clear. In a trial to elucidate the relation between skin inflammation, oxidative stress and antioxidants in vivo and in vitro data will be discussed. Using keratinocytes and fibroblasts producing oxidative stress either by UV light or xanthine – xanthine oxidase or tobacco smoke, the protection of antioxidants is evaluated. In vivo, in hairless mice, oxidative stress is estimated under different conditions as acute and chronic exposure to UV light or to tobacco smoke in both normal and diabetic skin, evaluating inflammation, oxidative stress and endogenous antioxidants. It seems that the dose of administered antioxidants is much important as a light pro-oxidant activity could be beneficial for the regeneration and protection of skin. In vivo, after UV light exposure, endogenous antioxidants as glutathion at the level of stratum corneum decreased while uric acid is increased. Administration of exogenous non antioxidant substances as lactic acid could also increase uric acid. Stratum corneum is much more susceptible to oxidative stress and antioxidant alterations in relation to living epidermis and dermis.
stress. SIRT3 knockdown could be a therapeutic strategy for colon cancer, since it improves the effectiveness of cytotoxic treatments. E-mail address: [email protected] (M. Torrens-Mas) Acknowledgements
Funding was provided by ISCIII (PI14/01434), MECD (FPU07042). http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.238
P-154
Diabetic skin and UV light: protection by Pinus halepensis pine bark extract Aggeliki Dimaki 1, Maria Kyriazi 1, Ioannis Sfiniadakis 2, Georgios Papaioannou 1, Lykourgos Klamarias 1, Vasileios Roussis 1, Michail Rallis 1 1 2
National and Kapodistrian University of Athens, Greece Naval Hospital of Athens, Greece
E-mail address: [email protected] (M. Rallis) Keywords: Diabetes mellitus; skin; UVR; pinus halepensis; vitamin C
http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.237
P-153
SIRT3 knockdown increases oxidative stress and sensitivity to cytotoxic treatments in SW620 cancer cells Margalida Torrens-Mas, Reyniel Hernández-López, Jorge Sastre-Serra, Pilar Roca, Jordi Oliver Grupo Multidisciplinar de Oncología Traslacional, Universitat de les Illes Balears, Institut Universitari d´Investigació en Ciències de la Salut (IUNICS), Instituto de Investigación Sanitaria de las Islas Baleares (IdISBa), Palma, Illes Balears, Spain; Ciber Fisiopatología Obesidad y Nutrición (CB06/03) Instituto Salud Carlos III, Madrid, Spain Keywords: Sirtuin 3; colon cancer; oxidative stress; oxaliplatin
Sirtuin 3 (SIRT3) is the major deacetylase in mitochondria and is activated under oxidative stress conditions. SIRT3 regulates mitochondrial metabolism and the antioxidant response to lower reactive oxygen species (ROS) production. Thus, SIRT3 could allow cells to counteract the effect of anticancer therapies, which increase ROS levels. The aim of this study was to determine whether SIRT3 knockdown in colon cancer cells could increase oxidative stress and therefore make them more sensitive to cytotoxic treatments. A stable SIRT3 silencing in SW620 human cell line was achieved through a specific shRNA. Expression of antioxidant enzymes and mitochondrial proteins was analyzed, as well as protein levels by Western Blot and MnSOD enzymatic activity. Cells were also treated with oxaliplatin to study ROS production by Amplex Reds. SIRT3 silencing resulted in a decrease of both antioxidant enzymes and proteins related to mitochondrial function, as well as a reduction in MnSOD activity. Furthermore, SIRT3 knockdown produced a significant increase in ROS production, which was greater with oxaliplatin treatment. Overall, these results suggest that SIRT3 is a key factor regulating mitochondria under oxidative
Diabetic skin is characterized by great sensitivity when compared to normal skin. Ultraviolet radiation is directly related to the oxidative stress affecting the skin. Diabetic skin, when compared to the normal one, is greatly damaged by ultraviolet radiation resulting in significant photoaging. Despite the fact that the longterm effect of UV light on normal skin has been partially investigated, for the diabetic skin data is missing. Antioxidants, such as Pinus halepensis seem to prevent photoaging. On the skin of hairless male diabetic mice the effects of UV radiation and the possible protection of Pinus halepensis pine bark extract topical application were studied, showing the significant antioxidant role of pine. E-mail address: [email protected] (M. Kyriazi) http://dx.doi.org/10.1016/j.freeradbiomed.2017.04.239
P-155
The role of aging and senescence on pancreatic β-cell function and proliferation Richard Kehm 1,2, Oliver Kluth 1,2, Annette Schürmann 1,2, Tilman Grune 1,2,3, Annika Höhn 1,2 1
German Institute of Human Nutrition (DIfE), Germany German Center for Diabetes Research (DZD), Germany 3 German Center for Cardiovascular Research (DZHK), Germany 2
Keywords: Aging; senescence; pancreatic β-cell; type-2-diabetes
Aging and age-related diseases are associated with the impairment of tissue function and their regenerative capacity. In terms of pancreatic β-cells, it is well known that insulin resistance in peripheral tissues increases with age due to metabolic changes caused by adiposity or physical inactivity. But in the presence of functional β-cells, insulin resistance alone is insufficient to lead to type-2-diabetes. However, in combination with an insulin