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Society of public analysts and other analytical results
l300K REVIEWS
296
VOL.
8’
(rg53)
A la fin de l’ouvragc SC trousc unc bibliographic assc% complt?tc qui doit dtre tr3s utile aux spdcialistcs. I,a IittGraturc dans cc domainc SC trouvant dans des hysiquea. agronomiques et cdramiques; cette revues bien divcrgentes: chimiqucs, bibliographic, soigneusement prdpnr P*c, sera Ia bienvenuc. C. J. VAX ~IIWWESI~URG (Delft)
SOCTJSTY
017
PUBf,IC
ANALYSTS AND OTWf2R AiSALYTICAL Biozo~icnC Mcllrotls Gvorrp
RESULTS
The Eighth Annual Gcncral Meeting of the J3iolo~ical Methods Grou of the Society of Public Anal sts and Other Analytical Chemrsts was held in the E hemical Society‘s Rooms, Bur rtngton Wousc, London W,r, on Thursday, December rrth, 1952, at 0.15 p.m. Dr. COLLIER, Chairman of the Group, was unavoidably delayed and his place was taken by Mr. A. L. I~ACHAI~ACI~. The following were elected Officers of the Group for the ensuing year: Clmhnan Dr. I-1. O. J. COLLIER Vice-CItairlnu?L Dr. I,. J. HAHRIS, P.R.T.C. 1.1072. .~ocvcznry & I-rcas2t?w Mr. S, A. Pr~~cr~, 33. SC., Walton Oaks Experimental Station, Vitamins Ltd., Dorking Road, Tadworth, Surrey. ‘l’hc Annual Goneral Meeting was followed at 6.30 pm. by an Ordinary Meeting of the Group, at which Dr. ‘I-f. 0. J. Cor,t~nn was in the Chair and thirty-two other members and guests were present. The following papers were presented and discussed: A Method of I&.tnti/yhg tire P~osc~ace OY .~~JISEPICL’ of S$lc$iiw “/f ‘* mtd Sple~rin l*u” ilr Scrrtr~b trsiq Gui92ca Pigs, by Raymond G~u:r:sxz and Josephine VAUGJSANMOlWAN. The variation c)f bleeding time in guinea 3igs living under controlled conditions was cstablishetl. Zn’ections were made of t1IL’ chloroform-soluble S lcnin “A” of normal serum and t ile acetone-soluble Splcnin “I3” from the serum o P patients with rl~cumatoitl arthritis, and the effect on the normat b&ding time noted. A clcscription of the method was given, followed by a discussion of the difficulties encountered and the reasons for carrying out the investigation. ‘I’& A ,bpticalion of Lavge PCntc MctJ~oris io Il~icrolriolo ?icrtL A .ssays of A~~libiotics mtrl I/ifuruin .P~ocf2tcfa, by Ii. A. LXIZS and J. I). 12. ’4'OOTI~ILL. Part I. Pvccision Assnys. By careful control of conclitions, and by dcvclo mcnt of suitable 12 x 12 designs, several unknowns can be simultaneously assayc s on a single Inrgc plate with 8n err0r of about I 0/*. This technique has been developed for assaying purified antibiotic and vitamin products when the error clue to impurities in the sample is known to bc less than I o/o. A feature of this assay method is that, bcsidcs the usual checks on validity, such as for parallelism and curvature. clircct cheeks on the accuracy of the dilutions employed arc available from the internal cviclcncc of each I z x 12 design. Part II. Rolrlinc /I zsays. To obtain assays with an error of about 5% by large plate methods suitable for routine purposes, s0mc of the cheeks on accuracy and validity in the fz x 12 designs have been eliminated, thus enabling 8 or 9 preparations to be compared on n single plate. For the routine clcsigas dealing wsth 8 preparations, borne simple arithmetic and a nomographic evaluation of the potency ratios enable the best estimates of these ratios tc, be objectively obtained. Replicate assays were csaminccl for consistency {using the error of the assay method as cietcrminrd over long periods) by means of a simple rough test,
296
VOL.
8’
(rg53)
A la fin de l’ouvragc SC trousc unc bibliographic assc% complt?tc qui doit dtre tr3s utile aux spdcialistcs. I,a IittGraturc dans cc domainc SC trouvant dans des hysiquea. agronomiques et cdramiques; cette revues bien divcrgentes: chimiqucs, bibliographic, soigneusement prdpnr P*c, sera Ia bienvenuc. C. J. VAX ~IIWWESI~URG (Delft)
SOCTJSTY
017
PUBf,IC
ANALYSTS AND OTWf2R AiSALYTICAL Biozo~icnC Mcllrotls Gvorrp
RESULTS
The Eighth Annual Gcncral Meeting of the J3iolo~ical Methods Grou of the Society of Public Anal sts and Other Analytical Chemrsts was held in the E hemical Society‘s Rooms, Bur rtngton Wousc, London W,r, on Thursday, December rrth, 1952, at 0.15 p.m. Dr. COLLIER, Chairman of the Group, was unavoidably delayed and his place was taken by Mr. A. L. I~ACHAI~ACI~. The following were elected Officers of the Group for the ensuing year: Clmhnan Dr. I-1. O. J. COLLIER Vice-CItairlnu?L Dr. I,. J. HAHRIS, P.R.T.C. 1.1072. .~ocvcznry & I-rcas2t?w Mr. S, A. Pr~~cr~, 33. SC., Walton Oaks Experimental Station, Vitamins Ltd., Dorking Road, Tadworth, Surrey. ‘l’hc Annual Goneral Meeting was followed at 6.30 pm. by an Ordinary Meeting of the Group, at which Dr. ‘I-f. 0. J. Cor,t~nn was in the Chair and thirty-two other members and guests were present. The following papers were presented and discussed: A Method of I&.tnti/yhg tire P~osc~ace OY .~~JISEPICL’ of S$lc$iiw “/f ‘* mtd Sple~rin l*u” ilr Scrrtr~b trsiq Gui92ca Pigs, by Raymond G~u:r:sxz and Josephine VAUGJSANMOlWAN. The variation c)f bleeding time in guinea 3igs living under controlled conditions was cstablishetl. Zn’ections were made of t1IL’ chloroform-soluble S lcnin “A” of normal serum and t ile acetone-soluble Splcnin “I3” from the serum o P patients with rl~cumatoitl arthritis, and the effect on the normat b&ding time noted. A clcscription of the method was given, followed by a discussion of the difficulties encountered and the reasons for carrying out the investigation. ‘I’& A ,bpticalion of Lavge PCntc MctJ~oris io Il~icrolriolo ?icrtL A .ssays of A~~libiotics mtrl I/ifuruin .P~ocf2tcfa, by Ii. A. LXIZS and J. I). 12. ’4'OOTI~ILL. Part I. Pvccision Assnys. By careful control of conclitions, and by dcvclo mcnt of suitable 12 x 12 designs, several unknowns can be simultaneously assayc s on a single Inrgc plate with 8n err0r of about I 0/*. This technique has been developed for assaying purified antibiotic and vitamin products when the error clue to impurities in the sample is known to bc less than I o/o. A feature of this assay method is that, bcsidcs the usual checks on validity, such as for parallelism and curvature. clircct cheeks on the accuracy of the dilutions employed arc available from the internal cviclcncc of each I z x 12 design. Part II. Rolrlinc /I zsays. To obtain assays with an error of about 5% by large plate methods suitable for routine purposes, s0mc of the cheeks on accuracy and validity in the fz x 12 designs have been eliminated, thus enabling 8 or 9 preparations to be compared on n single plate. For the routine clcsigas dealing wsth 8 preparations, borne simple arithmetic and a nomographic evaluation of the potency ratios enable the best estimates of these ratios tc, be objectively obtained. Replicate assays were csaminccl for consistency {using the error of the assay method as cietcrminrd over long periods) by means of a simple rough test,